Autoclaving: a modification in the preparation of tissue culture medium 199

1972 ◽  
Vol 18 (2) ◽  
pp. 272-273 ◽  
Author(s):  
C. P. Kenny ◽  
B. B. Diena ◽  
L. Greenberg
Keyword(s):  
Tissue Culture ◽  
Rhesus Monkey ◽  
Cell Lines ◽  
Hela Cells ◽  
Culture Medium ◽  
Kidney Tissue ◽  
Monkey Kidney ◽  
Tissue Culture Medium ◽  
Tissue Cell

HeLa cells and rhesus monkey kidney tissue cell lines can be propagated and maintained in medium 199 that has been sterilized by autoclaving. The choice of autoclaved M 199 is advantageous for some experimental procedures.

scholarly journals Differences in Levels of Secreted Locus of Enterocyte Effacement Proteins between Human Disease-Associated and Bovine Escherichia coli O157

2001 ◽  
Vol 69 (8) ◽  
pp. 5107-5114 ◽  
Author(s):  
Alan McNally ◽  
Andrew J. Roe ◽  
Sally Simpson ◽  
Fiona M. Thomson-Carter ◽  
D. E. Elaine Hoey ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Tissue Culture ◽  
Virulence Factors ◽  
Hela Cells ◽  
Human Disease ◽  
Culture Medium ◽  
Tissue Culture Medium ◽  
Human Origin ◽  
E Coli ◽  
Enterocyte Effacement

ABSTRACT Ongoing extensive epidemiological studies of verotoxin-carryingEscherichia coli O157 (stx + eae + ) have shown this bacterial pathogen to be common in cattle herds in the United States and the United Kingdom. However, the incidence of disease in humans due to this pathogen is still very low. This study set out to investigate if there is a difference between strains isolated from human disease cases and those isolated from asymptomatic cattle which would account for the low disease incidence of such a ubiquitous organism. The work presented here has compared human disease strains from both sporadic and outbreak cases with a cross-section, as defined by pulsed-field gel electrophoresis, of E. coli O157 strains from cattle. Human (n = 22) and bovine (n = 31) strains were genotyped for carriage of the genes for Shiga-like toxin types 1, 2, and 2c; E. coli secreted protein genesespA, espB, and espP; the enterohemolysin gene; eae (intimin); ast(enteroaggregative E. coli stable toxin [EAST]); and genes for common E. coli adhesins. Strains were also phenotyped for hemolysin, EspP, Tir, and EspD expression as well as production of actin and cytoskeletal rearrangement associated with attaching and effacing (A/E) lesions on HeLa cells. The genotyping confirmed that there was little difference between the two groups, including carriage of stx 2 andstx 2c, which was similar in both sets.ast alleles were confirmed to all contain mutations that would prevent EAST expression. espP mutations were found only in cattle strains (5 of 30). Clear differences were observed in the expression of locus of enterocyte effacement (LEE)-encoded factors between strains and in different media. EspD, as an indicator of LEE4 (espA, -B, and -D) expression, and Tir levels in supernatants were measured. Virtually all strains from both sources could produce EspD in Luria-Bertani broth, although at very different levels. Standard trichloroacetic acid precipitation of secreted proteins from tissue culture medium produced detectable levels of EspD from the majority of strains of human origin (15 of 20) compared with only a few (4 of 20) bovine strains (P < 0.001), which is indicative of much higher levels of protein secretion from the human strains. Addition of bovine serum albumin carrier protein before precipitation and enhanced detection techniques confirmed that EspD could be detected after growth in tissue culture medium for all strains, but levels from strains of human origin were on average 90-fold higher than those from strains of bovine origin. In general, levels of secretion also correlated with ability to form A/E lesions on HeLa cells, with only the high-level protein secretors in tissue culture medium exhibiting a localized adherence phenotype. This research shows significant differences between human- and bovine-derived E. coliO157 (stx + eae + ) strains and their production of certain LEE-encoded virulence factors. These data support the recent finding of Kim et al. (J. Kim, J. Nietfeldt, and A. K. Benson, Proc. Natl. Acad. Sci. USA 96:13288–13293, 1999) proposing different E. coli O157 lineages in cattle and humans and extend the differential to the regulation of virulence factors. Potentially only a subset of E. coli O157 isolates (stx + eae + ) in cattle may be capable of causing severe disease in humans.

scholarly journals Inmunización a Tripanosoma cruzi con antígenos tratados con detergentes

2017 ◽  
Vol 18 (2) ◽  
pp. 77-89
Author(s):  
Carlos Soria ◽  
Donald Dusanic
Keyword(s):  
Trypanosoma Cruzi ◽  
Rhesus Monkey ◽  
Cell Cultures ◽  
Kidney Tissue ◽  
Monkey Kidney ◽  
Tissue Cell ◽  
Survival Times ◽  
Treated Culture

C 3H/ Anf mice were immunized with Lubrol or cholate- treated culture forms of the Tuluhuén strain of Trypanosoma cruzi and subsequently challenged with the homologous bloodstream forms. The equivalent of 1x108 – 2x108 detergent-treated culture forms were injected intraperitoneally, intramuscularly or subcutaneously. The mice were challenged intraperitoneally with 1x105 – 2x105 bloddstream forms equivalent to 100-200LD50 administered 21 or 42 days after immunization. The effects of immunization were measured by recording survival times, noting blood and tissue parasitemias, and by attempting to recover parasites from the challenged mice in Rhesus monkey kidney tissue cell cultures and in irradiated mice.

ON TETHELIN AS A TISSUE CULTURE MEDIUM

1930 ◽  
Vol 1 (9) ◽  
pp. 289-290
Author(s):  
K. C. Richardson ◽  
E. S. Horning
Keyword(s):  
Tissue Culture ◽  
Culture Medium ◽  
Tissue Culture Medium

scholarly journals In Vitro Production of Erythropoietin by Kidneys Perfused With a Serum-free Solution

Blood ◽  
1974 ◽  
Vol 44 (1) ◽  
pp. 77-85 ◽  
Author(s):  
Allan J. Erslev
Keyword(s):  
Tissue Culture ◽  
Atmospheric Pressure ◽  
Culture Medium ◽  
Kidney Tissue ◽  
In Vitro Production ◽  
In Vitro Perfusion ◽  
Serum Free ◽  
Enzymatic Activation ◽  
Vitro Production

Abstract Normal rabbits exposed to 0.4 atmospheric pressure for 3 hr will generate about 40-60 U of erythropoietin during a subsequent 3-hr period. If the kidneys were removed from 3-hr hypoxic animals, washed carefully, and perfused for 3 hr by recirculation with a serum-tissue culture mixture, each kidney generated about 14 U of erythropoietin in vitro. Perfusion of normal kidneys did not result in the production of erythropoietin, and only small amounts were generated if the perfusate contained Puromycin. Three-hour hypoxic kidneys perfused for 3 hr with a serum-free tissue culture medium were found to generate about 8 U of erythropoietin per kidney and similar kidneys perfused with saline about 1 U. These results indicate that erythropoietin is synthesized by kidney tissue and not produced by enzymatic activation of a plasma substrate.

scholarly journals THE ANTIGENIC POTENCY OF EPIDEMIC INFLUENZA VIRUS FOLLOWING INACTIVATION BY ULTRAVIOLET RADIATION

1940 ◽  
Vol 72 (6) ◽  
pp. 729-745 ◽  
Author(s):  
Jonas E. Salk ◽  
G. I. Lavin ◽  
Thomas Francis
Keyword(s):  
Tissue Culture ◽  
Influenza Virus ◽  
Ultraviolet Radiation ◽  
Culture Medium ◽  
Tissue Culture Medium ◽  
Intraperitoneal Route ◽  
Active Virus ◽  
High Concentrations

A study of the antigenic potency of influenza virus inactivated by ultraviolet radiation has been made. Virus so inactivated is still capable of functioning as an immunizing agent when given to mice by the intraperitoneal route. In high concentrations inactivated virus appears to be nearly as effective as active virus but when quantitative comparisons of the immunity induced by different dilutions are made, it is seen that a hundredfold loss in immunizing capacity occurs during inactivation. Virus in suspensions prepared from the lungs of infected mice is inactivated more rapidly than virus in tissue culture medium. A standard for the comparison of vaccines of epidemic influenza virus is proposed.

scholarly journals Enterovirus 70 Receptor Utilization Is Controlled by Capsid Residues That Also Regulate Host Range and Cytopathogenicity

2007 ◽  
Vol 81 (16) ◽  
pp. 8648-8655 ◽  
Author(s):  
Melissa Stewart Kim ◽  
Vincent R. Racaniello
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Rhesus Monkey ◽  
Host Range ◽  
Hela Cells ◽  
Lytic Replication ◽  
Monkey Kidney ◽  
Kidney Cells ◽  
Cytopathic Effects ◽  
Axis Of Symmetry

ABSTRACT Enterovirus type 70, an etiologic agent of acute hemorrhagic conjunctivitis, may bind different cellular receptors depending on cell type. To understand how EV70-receptor interaction is controlled, we studied two variants of the virus with distinct receptor utilization. EV70-Rmk, derived by passage in rhesus monkey kidney cells, replicates poorly in HeLa cells and does not cause cytopathic effects. Decay accelerating factor (DAF) is not a cell receptor for EV70-Rmk. Passage of EV70-Rmk in HeLa cells lead to isolation of EV70-Dne, which does not replicate in rhesus monkey kidney cells but grows to high titers in HeLa cells and causes cytopathic effects. DAF is sufficient for cell entry of EV70-Dne. EV70-Rmk replicates in human eye and brain-derived cell lines, whereas the Dne strain replicates only in HeLa cells and in conjunctiva-derived 15C4 cells. The two EV70 strains differ by five amino acid changes in the viral capsid. Single substitution of four of the five EV70-Rmk amino acids with the residue from EV70-Dne leads to lytic replication in HeLa cells. Conversely, substitution of any of the five EV70-Dne amino acids with the EV70-Rmk amino acid does not alter replication in HeLa cells. Three of these capsid amino acids are predicted to be located in the canyon encircling the fivefold axis of symmetry, one amino acid is found at the fivefold axis of symmetry, and one is located the interior of the capsid. The five EV70 residues define a region of the capsid that controls viral host range, DAF utilization, and cytopathogenicity.

Formation of poliovirus in monkey kidney tissue culture cells

Virology ◽  
1962 ◽  
Vol 16 (3) ◽  
pp. 325-333 ◽  
Author(s):  
Heather Donald Mayor ◽  
Liane E. Jordan
Keyword(s):  
Tissue Culture ◽  
Kidney Tissue ◽  
Monkey Kidney ◽  
Culture Cells ◽  
Tissue Culture Cells
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