scholarly journals DETERMINATION OF THE RELATIONSHIP AMONG RAW COW MILK COMPOSITION AND SOMATIC CELL COUNT OBTAİN FROM MILK COLLECTION CENTRES

2018 ◽  
Vol 2 (2) ◽  
pp. 23-30
Author(s):  
Ayşe Gülin SEZER ◽  
Galin Y. IVANOV ◽  
Ertugrul BİLGÜCÜ
Keyword(s):  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Milk Composition ◽  
Cow Milk ◽  
The Relationship

scholarly journals Differential Somatic Cell Count as a Marker for Changes of Milk Composition in Cows with Very Low Somatic Cell Count

Animals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 604 ◽  
Author(s):  
Alfonso Zecconi ◽  
Francesca Dell’Orco ◽  
Diego Vairani ◽  
Nicoletta Rizzi ◽  
Micaela Cipolla ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Cell Count ◽  
Milk Fat ◽  
Somatic Cell Count ◽  
Milk Composition ◽  
Cow Milk ◽  
Udder Health ◽  
Lactating Cows ◽  
Accuracy Of Measurements ◽  
The Relationship

The recent availability of a high-throughput milk analyzer performing a partial differential somatic cell count (DSCC) opened new opportunities in investigations on bovine udder health. This analyzer has a potential limitation on the accuracy of measurements when the somatic cell count (SCC) is below 50,000 cells/mL, values characterizing a good proportion of lactating cows in many herds. We obtained data for cows below this threshold, assessed the repeatability of these measurements and investigated the relationship between DSCC and udder health, milk composition and yield. Overall, 3022 cow milk test records performed on a Fossomatic™ 7/DC (Foss A/S, Hillerød, Denmark) were considered; 901 of them had an SCC ≤ 50,000 cells/mL. These latter samples were analyzed by qPCR to identify the presence of bacteria. Overall, 20.75% of the samples (187) were positive. However, the health status did not have any significant association with DSCC. The analysis of the association of DSCC on milk fat, protein and casein showed a significant decrease in their proportions as the DSCC increased, whereas it was not observed for milk yield and lactose. Therefore, DSCC in very low SCC cows may be suggested as a marker to identify early changes in milk composition.

scholarly journals Somatic cell count obtained by Ekomilk Scan® and correlations with other methods of analysis

2018 ◽  
Vol 48 (6) ◽  
Author(s):  
Ana Carolina Siqueira Gonçalves ◽  
Luiz Carlos Roma Júnior ◽  
Rafaela Torrezan Privatti ◽  
Márcia Saladini Vieira Salles ◽  
Claudia Cristina Paro de Paz ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Milk Composition ◽  
Cow Milk ◽  
Direct Microscopy ◽  
Reference Flow ◽  
Northeastern State ◽  
Repeatability And Reproducibility

ABSTRACT: The study aimed to evaluate the equipment Ekomilk Scan® as an alternative to somatic cell count (SCC) in milk. For this individual cow milk samples of various ages and different stages of lactation in northeastern state of São Paulo region were collected. The analyzes performed were divided into variables related to the equipment: repeatability and reproducibility, and variables that could influencing the results as: use of preservatives, temperature, time between collection and analysis, breed and milk composition, besides analysis to relate the Ekomilk Scan® with the standard method-direct microscopy and reference-flow cytometry. As the result, for samples analysis, it shouldn’t be added preservative and these should be conducted on the same day of collection; however, temperature sample did not significantly influence results. Furthermore, Ekomilk Scan® did not show good correlation of results with the method of direct microscopy; however, it was necessary to generate equations for a positive correlation between flow cytometry and Ekomilk Scan®. Therefore, it is concluded that the equipment tested is not accurate but it can be an alternative for SCC monitoring in productive units since it uses calibration equations of results.

Evaluation of somatic cell count from cow milk composition to response of treatment with microwave

2014 ◽  
Vol 185 ◽  
pp. S81
Author(s):  
Iuliana Cretescu ◽  
Mircea Dan Petrescu ◽  
Ioan Pet ◽  
Mihaela Ostan ◽  
Elena Pet ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Milk Composition ◽  
Cow Milk

scholarly journals Somatic cell count and chemical composition of milk in naturally BLV-infected cows with different phenotypes of blood leukocyte acid phosphatase

2006 ◽  
Vol 49 (1) ◽  
pp. 17-28
Author(s):  
B. Bojarojć-Nosowicz ◽  
E. Kaczmarczyk
Keyword(s):  
Acid Phosphatase ◽  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
First Trimester ◽  
Milk Composition ◽  
Blood Leukocytes ◽  
Cell Counts ◽  
Blood Leukocyte ◽  
The Relationship

Abstract. Effectiveness of the methods applied to control mastitis is low. Therefore, indices are sought which could improve cow immunity to udder pathogens. The aim of the study was to determine the relationship between the polymorphism of blood leukocyte acid phosphatase (AcP), BLV infection, the somatic cell counts and the milk composition in the first trimester of lactation. Studies were performed on a population of 65 Black-and-White cows, aged 3–6 years, from a leukemia-dominated herd. Enzootic bovine leukemia was diagnosed with ELISA and PCR tests. The following analyses were performed: the contents of total protein, lactose, dry matter, somatic cell count in milk as well as microbiological analyses. The obtained results indicate the occurrence of an association between a natural BLV-infection and mammary gland secretion disturbances in cows, whereas the relationship with the acid phosphatase polymorphism is not explicit. The obtained results encourage the continuation of studies into the role of blood leukocytes AcP in the pathogenesis of mastitis.

scholarly journals Somatic cell count in the diagnosis of subclinical mastitis in sheep of different breeds

2018 ◽  
Vol 39 (4) ◽  
pp. 1555
Author(s):  
Luiz Francisco Zafalon ◽  
Raul Costa Mascarenhas Santana ◽  
Sérgio Novita Esteves ◽  
Guilherme Aparecido Fim Júnior
Keyword(s):  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Subclinical Mastitis ◽  
Control Measures ◽  
Coagulase Negative Staphylococci ◽  
Stage Of Lactation ◽  
Santa Inês ◽  
Etiological Agents

The aims of this study were to determine the occurrence of subclinical mastitis in sheep of different breeds and the values for somatic cell count (SCC) in milk for the diagnosis of the disease at lactation and weaning, a fundamental prerequisite for identifying animals in need of control measures. Milk samples were obtained from 1,457 mammary halves of Santa Inês, Texel, Ile de France, and Dorper sheep at two different periods, during the second week of lactation and at weaning. After teats antisepsis, the samples were collected, and identification of the infectious etiology of mastitis and determination of SCC were performed. Microorganisms were identified in 117/762 (15.3%) mammary halves in the second week of lactation and in 86/694 (12.4%) at weaning. Coagulase-negative staphylococci (CoNS) were the etiological agents with the highest incidence alone and in association with other microorganisms, with percentages of 58.1% and 60.6%, respectively. The Santa Inês presented a higher incidence of subclinical mastitis when compared to the other breeds. The cut-off values of SCC for subclinical mastitis were determined at both sampling periods and varied according to stage of lactation, as well breed. These results illustrate the lack of a universal value that can be used for the diagnosis of mastitis and suggests the need for permanent follow-up in herds in order to control the disease.

scholarly journals Prediction of total quarter milk somatic cell counts based on foremilk sampling

2009 ◽  
Vol 76 (3) ◽  
pp. 326-330 ◽  
Author(s):  
Olga Wellnitz ◽  
Marcus G Doherr ◽  
Marta Woloszyn ◽  
Rupert M Bruckmaier
Keyword(s):  
Dairy Cows ◽  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Practical Reasons ◽  
Udder Health ◽  
Somatic Cell Counts ◽  
Cell Numbers ◽  
Cell Counts

Determination of somatic cell count (SCC) is used worldwide in dairy practice to describe the hygienic status of the milk and the udder health of cows. When SCC is tested on a quarter level to detect single quarters with high SCC levels of cows for practical reasons, mostly foremilk samples after prestimulation (i.e. cleaning of the udder) are used. However, SCC is usually different in different milk fractions. Therefore, the goal of this study was the investigation of the use of foremilk samples for the estimation of total quarter SCC. A total of 378 milkings in 19 dairy cows were performed with a special milking device to drain quarter milk separately. Foremilk samples were taken after udder stimulation and before cluster attachment. SCC was measured in foremilk samples and in total quarter milk. Total quarter milk SCC could not be predicted precisely from foremilk SCC measurements. At relatively high foremilk SCC levels (>300×103 cells/ml) foremilk SCC were higher than total quarter milk. At around (50–300)×103 cells/ml foremilk and total quarter SCC did not differ considerably. Most interestingly, if foremilk SCC was lower than 50×103 cells/ml the total quarter SCC was higher than foremilk SCC. In addition, individual cows showed dramatic variations in foremilk SCC that were not very well related to total quarter milk SCC. In conclusion, foremilk samples are useful to detect high quarter milk SCC to recognize possibly infected quarters, only if precise cell counts are not required. However, foremilk samples can be deceptive if very low cell numbers are to be detected.

scholarly journals Effect of storage and separation of milk at udder quarter level on milk composition, proteolysis, and coagulation properties in relation to somatic cell count

2011 ◽  
Vol 94 (11) ◽  
pp. 5341-5349 ◽  
Author(s):  
L. Forsbäck ◽  
H. Lindmark-Månsson ◽  
K. Svennersten-Sjaunja ◽  
L. Bach Larsen ◽  
A. Andrén
Keyword(s):  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Milk Composition

scholarly journals Fluorescence microscopy methods for the determination of somatic cell count in raw cow’s milk

2016 ◽  
Vol 61 (No. 11) ◽  
pp. 612-622 ◽  
Author(s):  
P. Zajac ◽  
S. Zubricka ◽  
J. Capla ◽  
L. Zelenakova
Keyword(s):  
Fluorescence Microscopy ◽  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Cow’S Milk ◽  
Cow's Milk ◽  
Raw Cow’S Milk
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