scholarly journals Serological and biochemical characteristics of virulence plasmid of Yersinia enterocolitica isolates from chicken in the Islamic Republic of Iran

2000 ◽  
Vol 6 (2-3) ◽  
pp. 409-415
Author(s):  
M. Motallebi
Keyword(s):  
Human Serum ◽  
Yersinia Enterocolitica ◽  
Experimental Infection ◽  
Virulence Plasmid ◽  
Plasmid Curing ◽  
Islamic Republic ◽  
Biochemical Characteristics ◽  
Calcium Dependency ◽  
Islamic Republic Of Iran ◽  
Pathogenic Yersinia

Pathogenic Yersinia enterocolitica harbour plasmid that is essential for virulence. We studied the characteristics of virulence plasmid using serological, biochemical and bioassay tests in Y. enterocolitica isolates of chicken using plasmid curing. Plasmid-cured isogenic derivatives [2029c and 2150c] were obtained from two isolates of Y. enterocolitica [RTCC 2029 and RTCC 2150]. The results demonstrated that plasmid-bearing isolates [2029 and 2150] were human-serum-resistant when grown at 37°C, but were sensitive when grown at 25°C, whereas plasmid-cured isolates [2029c and 2150c] were sensitive when grown at both temperatures. Also autoagglutination, calcium-dependency tests and experimental infection in mice demonstrated that these phenotypes were associated with the virulence plasmid

Prevalence of Pathogenic Yersinia enterocolitica Strains on Liver Surfaces of Pigs and Their Antimicrobial Susceptibility

2010 ◽  
Vol 73 (9) ◽  
pp. 1680-1683 ◽  
Author(s):  
A. von ALTROCK ◽  
U. ROESLER ◽  
R. MERLE ◽  
K.-H. WALDMANN
Keyword(s):  
Yersinia Enterocolitica ◽  
Antimicrobial Susceptibility ◽  
Virulence Gene ◽  
Resistance Testing ◽  
Dilution Method ◽  
Virulence Plasmid ◽  
Lower Saxony ◽  
Resistant Strains ◽  
Enterocolitica Infection ◽  
Pathogenic Yersinia

A study to determine the occurrence of pathogenic Yersinia enterocolitica on surfaces of slaughtered pig livers and the antimicrobial resistant pattern of the isolates was carried out in a slaughterhouse in Lower Saxony, Germany. During the slaughtering process, 1,500 surfaces of pig livers from 50 fattening herds were swabbed in order to isolate and characterize Y. enterocolitica isolates by serotyping, detecting the virulence plasmid coding the yopT gene, and resistance testing. Of the livers tested, 4.7% were positive for Y. enterocolitica O:3, which was the only identified serotype. The virulence gene yopT was found in 90.0% of these isolates. Antimicrobial susceptibility was tested by the broth dilution method, and the MICs were determined for 13 antimicrobials. All isolates were resistant to ampicillin and sulfamethoxazole but were susceptible to ciprofloxacin, nalidixic acid, gentamicin, ceftiofur, tetracycline, kanamycin, cefotaxime, and chlorphenicol. Up to now, resistance to florfenicol has always been described in combination with resistance to chloramphenicol. In the present study, 15.3% of the isolates were resistant to florfenicol, while no chloramphenicol-resistant strains could be identified. Multiresistance to three or more antimicrobials was detected in 22 strains (27.3%). Nevertheless, third-generation cephalosporines or fluoroquinolones, which were recommended for extraintestinal Y. enterocolitica infection in humans, were not affected.

Differences in Heat Resistance among Pathogenic Yersinia enterocolitica Depended on Growth Temperature and Serotype

2005 ◽  
Vol 68 (5) ◽  
pp. 1081-1082 ◽  
Author(s):  
H. HAYASHIDANI ◽  
Y. HARA-KUDO ◽  
S. KINOSHITA ◽  
K. SAEKI ◽  
A. T. OKATANI ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Heat Resistance ◽  
Outer Membrane ◽  
Yersinia Enterocolitica ◽  
Outer Membrane Protein ◽  
Growth Temperature ◽  
Virulence Plasmid ◽  
Temperature Dependent ◽  
Virulence Plasmids ◽  
Pathogenic Yersinia

To gain a better understanding about the effect of growth temperature on heat resistance of Yersinia enterocolitica, we determined decimal reduction times at 60°C (D60-values) for O:3; O:5,27; O:8; and O:9 strains harboring virulence plasmid coding for Yersinia outer membrane protein and experimentally virulence plasmid–deleted strains after they were grown to stationary phase at 7, 25, or 37°C. Bacteria were inoculated into Trypticase soy broth and were incubated at several temperatures. D60-values of O:3; O:5,27; and O:8 strains were larger when they were grown at 37°C than at 7 or 25°C, despite the presence or absence of virulence plasmids. However, similar D60-values were observed in O:9 strains, despite growth at 7, 25, or 37°C. The results indicate two types of Y. enterocolitica strains, growth temperature–dependent and –independent, and a Yersinia outer membrane protein that is not directly involved in growth temperature–dependent heat resistance.

Shiga toxin-producing bacteria as emerging enteric pathogens associated with outbreaks of foodborne illness in the Islamic Republic of Iran

2020 ◽  
Vol 28 (8) ◽  
pp. 976-981
Author(s):  
Elahe Tajeddin ◽  
Leila Ganji ◽  
Zahra Hasani ◽  
Fahimeh Sadat Ghoalm Mostafaei ◽  
Masoumeh Azimirad
Keyword(s):  
Shiga Toxin ◽  
Foodborne Illness ◽  
Enteric Pathogens ◽  
Islamic Republic ◽  
Islamic Republic Of Iran
Sign in / Sign up

Export Citation Format

Share Document