scholarly journals Estudio de la prevalencia de Salmonella spp y Escherichia coli en huevos comerciales para consumo humano en el cantón Ibarra, para la creación de un banco de recursos microbianos de la PUCE-SI

AXIOMA ◽  
2021 ◽  
Vol 1 (25) ◽  
pp. 11-16
Author(s):  
Santiago Mafla
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Sp ◽  
Salmonella Spp ◽  
Acinetobacter Sp

Las enfermedades transmitidas por alimentos (ETA’s) son un problema importante en la salud pública y la prevalencia de las mismas permiten conocer la calidad higiénico-sanitaria de los alimentos. Se realizó un estudio descriptivo de corte transversal, en el cuál se muestrearon aleatoriamente 8 sitios de expendio, de los cuales 4 corresponden al denominado sector A (cadenas comerciales) y, los otros 4 del sector B (distribuidores mayoristas) de huevo comercial en la ciudad de Ibarra, es así que se escogieron al azar 10 huevos por sitio de expendio, dando así un total de 80 huevos estudiados. De cada huevo se sustrajo 3 muestras: clara, yema y cascarón; obteniendo un total de 240 muestras que se repartieron en dos grupos, cultivos para Salmonella spp., y Escherichia coli., obteniendo un total de 480 muestras procesadas para cultivo bacteriano aplicando los métodos de la Norma Técnica Ecuatoriana INEN 1529-15 e INEN 1529-8 respectivamente, para el control microbiológico de alimentos. Como resultado del análisis microbiológico se obtuvo un total de 18 muestras positivas, 10 para Salmonella spp., y 8 para Escherichia coli, con ello se procedió a realizar el aislamiento de las colonias en caldo nutritivo para posteriormente realizar la extracción de ADN. Terminada esta etapa se realizó la PCR para la amplificación del gen ADNr 16s, y con ello realizar la técnica RFLP para la detección de polimorfismos. En conclusión, se obtuvo una prevalencia del 4,16% y 3,33% para Salmonella spp., y Escherichia coli; siendo el sector B (distribuidores mayoristas) el de mayor prevalencia, además mediante la secuenciación se detectaron otras especies bacterianas correspondientes a: Pseudomonas sp, Acinetobacter sp, Enterobacter sp. Se identificó dos serotipos para Salmonella spp: S. Enteritidis y S. Typhimurium. El serotipo identificado de la bacteria Escherichia coli fue O157:H7.

scholarly journals 2475. Incidence of Multidrug-Resistant, Extensively Drug-Resistant and Pandrug-Resistant Gram-Negative Bacteria in Brazilian Intensive Care Units

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S857-S857
Author(s):  
Gregory Lauar Souza ◽  
Rhayssa Fernanda de Andrade Rocha ◽  
Andressa Do nascimento Silveira ◽  
Handerson Dias Duarte de Carvalho ◽  
Cristóvão D M Oliveira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Intensive Care ◽  
Multidrug Resistant ◽  
Resistance Rate ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Pseudomonas Sp ◽  
Gram Negative ◽  
Extensively Drug Resistant ◽  
Acinetobacter Sp

Abstract Background The Centers for Disease Control and Prevention (CDC) proposed standard definitions for acquired resistance in bacterias. Resistant bacteria were categorized as multidrug-resistant (MDR), extensively drug-resistant (XDR) and pandrug-resistant (PDR). This study describes the incidence of Gram-negative MDR, XDR and PDR in 12 private and adult intensive care units (ICU’s) from Belo Horizonte, Minas Gerais, the sixth most populated city in Brazil, with approximately 3 million inhabitants. Methods Data were collected between January/2013 to December/2017 from 12 ICU’s. The hospitals used prospective healthcare-associated infections (HAI) surveillance protocols, in accordance to the CDC. Antimicrobial resistance from six Gram-negatives, causing nosocomial infections, were evaluated: Acinetobacter sp., Klebsiella sp., Proteus sp., Enterobacter sp., Escherichia coli, and Pseudomonas sp.. We computed the three categories of drug-resistance (MDR+XDR+PDR) to define benchmarks for the resistance rate of each Gram-negative evaluated. Benchmarks were defined as the superior limits of 95% confidence interval for the resistance rate. Results After a 5 year surveillance, 6,242 HAI strains were tested: no pandrug-resistant bacteria (PDR) was found. Acinetobacter sp. was the most resistant Gram-negative: 206 strains from 1,858 were XDR (11%), and 1,638 were MDR (88%). Pseudomonas sp.: 41/1,159 = 3.53% XDR; 180/1,159 = 15.53% MDR. Klebsiella sp.: 2/1,566 = 0,1% XDR; 813/1,566 = 52% MDR. Proteus sp.: 0/507 = 0% XDR; 163/507 = 32% MDR. Enterobacter sp.: 0/471 = 0% XDR; 148/471 = 31% MDR. Escherichia coli: 0/681 = 0% XDR; 157/681 = 23% MDR. Benchmarks for the global resistance rate of each Gram-negative (MDR+XDR+PDR): Acinetobacter sp. = 92%; Klebsiella sp. = 62%; Proteus sp. = 40%; Enterobacter sp. = 48%; Escherichia coli = 33%; Pseudomonas sp. = 30%. Conclusion This study has calculated the incidence of Gram-negative MDR, XDR and PDR, and found a higher incidence of MDR Acinetobacter sp., with an 88% multiresistance rate. Henceforth, developing countries healthcare institutions must be aware of an increased risk of infection by Acinetobacter sp.. Benchmarks have been defined, and can be used as indicators for healthcare assessment. Disclosures All authors: No reported disclosures.

Evaluating the fate of Escherichia coli O157:H7 and Salmonella spp. on cucumbers

2021 ◽  
pp. 103830
Author(s):  
Arícia Possas ◽  
Guiomar Denisse Posada-Izquierdo ◽  
Gonzalo Zurera ◽  
Fernando Pérez-Rodríguez
Keyword(s):  
Escherichia Coli ◽  
Escherichia Coli O157 ◽  
Salmonella Spp

scholarly journals Caracterizações microbiológica e físico-química de pólens armazenados por abelhas sem ferrão

2018 ◽  
Vol 21 (0) ◽  
Author(s):  
Marivalda Figueredo Santa Bárbara ◽  
Cerilene Santiago Machado ◽  
Geni da Silva Sodré ◽  
Fabiane de Lima Silva ◽  
Carlos Alfredo Lopes de Carvalho
Keyword(s):  
Escherichia Coli ◽  
Salmonella Spp ◽  
Melipona Subnitida

Resumo O estudo foi realizado com o objetivo de verificar tanto a condição microbiológica quanto as diferenças físico-químicas existentes em pólens armazenados por diferentes espécies de abelhas sem ferrão no Nordeste do Brasil. Os pólens armazenados foram coletados nas colônias mantidas em meliponários das seguintes espécies: Melipona subnitida, M.scutellaris, M. mandacaia, Scaptotrigona sp. e Frieseomellita varia. Com relação às análises de qualidade microbiológica, as mesmas foram realizadas em micro-organismos indicadores de qualidade comercial (mesófilos, bolores e leveduras), qualidade sanitária (Staphylococcus coagulase positiva, coliformes fecais e Escherichia coli), e de segurança (esporos de Clostridium sulfito redutores e Salmonella spp.). Para os parâmetros físico-químicos, os mesmos foram avaliados de acordo com a exigência do Regulamento Técnico (RT) de qualidade para pólen apícola, e também para compostos fenólicos. Todas as amostras apresentaram condição microbiológica de acordo com as recomendações aceitáveis para o consumo humano. Os resultados confirmaram as diferenças na composição físico-química do pólen armazenado das cinco espécies; no entanto, houve formação de três grupos de pólens armazenados com base nos parâmetros físico-químicos. As amostras da espécie M. scutellaris apresentaram conteúdo de compostos fenólicos cinco vezes mais elevado aos outros méis das espécies avaliadas. Logo, este estudo permitiu conhecer a composição dos pólens armazenados por abelhas sem ferrão, por conseguinte reforçando a necessidade de reformular o Regulamento Técnico (RT) existente no Brasil para pólen apícola, de forma a abranger os pólens armazenados das espécies nativas.

scholarly journals Prevalence and antimicrobial resistance of Salmonella spp. and Escherichia coli isolated from pigs at slaughterhouses in Korea

2011 ◽  
Vol 5 (7) ◽  
pp. 823-830 ◽  
Author(s):  
Bi Kim Hyo ◽  
Baek Hyun ◽  
Lee SooJin ◽  
Jang YangHo ◽  
Jung SukChan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Salmonella Spp

scholarly journals Assessment of Hazard Analysis Critical Control Point (HACCP) of Fast Food (Momo) from Restaurants of Kathmandu City

1970 ◽  
Vol 9 ◽  
pp. 49-56
Author(s):  
Poonam Thapa ◽  
Anjana Singh ◽  
Tika Bahadur Karki
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Fast Food ◽  
Proper Time ◽  
Hazard Analysis ◽  
Control Point ◽  
Salmonella Spp ◽  
Coliform Count ◽  
Critical Control Point ◽  
Mold Count

Hazard analysis critical control point (HACCP) module was prepared for one of the most popular fast food momo (chicken momo and buff momo). For this, hazard analysis was conducted in eight different restaurants of Katmandu city by observing all the steps of preparation, monitoring time-temperature throughout the preparation process and collecting samples of different stages of these food. The samples were assessed for total aerobic mesophilic count (TAMC), total coliform count, total Staphylococcus aureus count, total yeast and mold count, detection of Salmonella spp. and Escherichia coli. During preparation of chicken momo, the highest TAMC, yeast and mold count, coliform and S. aureus count were found to be 2.8 × 106cfu/g, 2.1 × 103cfu/g, 1.92 × 105cfu/g and 3.4 × 103cfu/g respectively. While preparation of buff momo, the highest TAMC, yeast and mold count, coliform count and S. aureus count were found to be 2.82 × 106cfu/g, 1.9 × 103cfu/g, 2.1 × 105cfu/g and 2.8 × 103cfu/g respectively. These values and near to these values too were obtained from the samples of pickles, spices, raw momo, mixture of minced meat with spices and raw meat. The organisms originally present in the raw materials were subsequently transmitted to all the preparatory stages but was not observed after steaming and hence the final steamed product of both kinds of momo were free from microorganisms. Thus from the above findings, it was concluded that steaming was the main critical control point (CCP), which if done for proper time and temperature, can eliminate all the contaminating organisms. Key words: coliform count, critical control point, hazard analysis, Staphylococcus aureus, Escherichia coli, Salmonella spp DOI: 10.3126/njst.v9i0.3164 Nepal Journal of Science and Technology 9 (2008) 49-56

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