scholarly journals CHARACTERIZATION OF ANTIFUNGAL ACTIVITY OF THE VARIOUS ACTIVE CONSTITUENTS OF COLEUS

2015 ◽  
Vol 2 (1) ◽  
pp. 125-127
Author(s):  
Ranjith Santhosh Kumar D.S ◽  
Lakshman Kumar B
Keyword(s):  
Methanolic Extract ◽  
Pharmaceutical Preparations ◽  
The Other ◽  
Coleus Forskohlii ◽  
Volatile Oils ◽  
Antifungal Effect ◽  
Active Constituents ◽  
Anti Fungal ◽  
Layer Chromatography

The aim of this study was to evaluate the anti-fungal effect of different solvent extracts of Coleus forskohlii which contains minor diterpenoids, deacetylforskohlin, 9-deoxyforskohlin, 1,9-deoxyforskohlin, 1,9-dideoxy-7-deacetylforskohlin, and four other diterpenoids, along with alkaloids and volatile oils. The solvent extracts included both polarized and non-polarized solvents like methanol, ethylacetate, benzene, chloroform, water, hexane, acetone and ethanol. As a result of this study, the antifungal effect was best observed with the methanolic extract against Candida albicans in comparison to the other extracts. This study also revealed that apart from forskohlin, which was confirmed by Thin Layer Chromatography, other compounds that were extracted with methanol are required to produce the antifungal effect and can be taken forward to pharmaceutical preparations.

Extraction and Characterization of Gibberellins from Hordeum vulgare L. Seedlings

1974 ◽  
Vol 1 (2) ◽  
pp. 183 ◽  
Author(s):  
KF Faull ◽  
BG Coombe ◽  
LG Paleg
Keyword(s):  
Mass Spectrometry ◽  
Dry Weight ◽  
Paper Electrophoresis ◽  
The Other ◽  
Gas Chromatography Mass Spectrometry ◽  
Gas Liquid Chromatography ◽  
Fresh Weight ◽  
Hordeum Vulgare L ◽  
Layer Chromatography

Two gibberellins, one GA1-like, the other GA3-like, were identified in the extracts of roots and tops of 8-,11- and 15-day-old barley seedlings by paper chromatography, paper electrophoresis, thin-layer chromatography, gas-liquid chromatography and bioassay procedures, followed by combined gas chromatography-mass spectrometry. The amounts of gibberellins in the seedlings ranged from 7 to 11 ng per plant. The concentrations of gibberellins in the seedlings were 32-320 ng/g dry weight and 5-28 ng/g fresh weight; concentrations in the roots were higher than those in the shoots.

CHARACTERIZATION OF ACTIVE COMPOUNDS FROM CITRUS PEELS BY-PRODUCTS USING CHROMATOGRAPHIC AND OPTIC METHODS

2017 ◽  
Vol 31 (2) ◽  
pp. 41-47
Author(s):  
Cristina Mihali ◽  
◽  
Thomas Dippong ◽  
Anca Dumuţa ◽  
◽  
...  
Keyword(s):  
Visible Region ◽  
Citrus Fruit ◽  
Volatile Oils ◽  
Complex Composition ◽  
Citrus Peels ◽  
Layer Chromatography ◽  
Derivatives Of ◽  
By Products ◽  
Extracting Agents

Citrus fruits such as orange, grapefruit, lemon and other citrus fruit are widely cultivated and processed generating large amounts of by-products. Citrus peels contain various compounds of economic importance such as volatile oils, flavonoids, polyphenols, carotenoids etc. It is essential to valorize the wastes of citrus processing. In this purpose, peels of orange, grapefruit and lemon, fresh and dried were subjected to extraction using trichloromethane and dichloromethane as extracting agents and analyzed by thin layer chromatography on silica gel plates. Visualization at 366 nm and 254 nm showed a complex composition of extracts that contain terpenes, oxygen heterocyclic substances derivatives of coumarin and carotenoids. The UV-VIS spectra of the extracts were recorded showing absorption in UV and Visible region.

scholarly journals Characterization of the Influenza Virus Inhibiting Fractions of Bergenia ligulata

2012 ◽  
Vol 12 ◽  
pp. 290-295
Author(s):  
M Rajbhandari ◽  
Th Schoepke
Keyword(s):  
Influenza Virus ◽  
Antiviral Activity ◽  
Thin Layer ◽  
Plant Extracts ◽  
Condensed Tannins ◽  
Methanolic Extract ◽  
Active Constituents ◽  
Influenza Virus A ◽  
Bio Assay

In the course of screening of plant extracts for anti-influenza viral activity, 50% aqueous methanolic extract exhibited antiviral activity against influenza virus A. Bio-assay guided fractionation of the extract led to the indication of the presence of condensed tannins as the active constituents. This was confirmed by chemical tests as well as by thin layer chromatographic comparison with known tannin containing plant extracts. The active fractions were also analysed for the presence of carbohydrates by gas chromatography.DOI: http://dx.doi.org/10.3126/njst.v12i0.6515 Nepal Journal of Science and Technology 12 (2011) 290-295

scholarly journals Bio-Guided Fractionation of Retama raetam (Forssk.) Webb & Berthel Polar Extracts

Molecules ◽  
2021 ◽  
Vol 26 (19) ◽  
pp. 5800
Author(s):  
Mariem Saada ◽  
Hanen Wasli ◽  
Inès Jallali ◽  
Rim Kboubi ◽  
Karl Girard-Lalancette ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Antibacterial Properties ◽  
Chemical Characterization ◽  
Polar Compounds ◽  
Bioactive Molecules ◽  
Prenylated Flavonoids ◽  
Retama Raetam ◽  
Polar Extracts ◽  
Layer Chromatography

The fractionation of the methanolic extract (MeOH-E) of Retama raetam (Forssk.) Webb & Berthel and further analysis by thin layer chromatography resulted in four fractions (F1, F2, F3 and F4) that, in parallel with the MeOH-E, were screened for antioxidant, cytotoxic, antidiabetic and antibacterial properties. In addition, chemical characterization of their bioactive molecules was performed using LC-DAD-ESI/MSn. The results indicated that F3 was the most promising regarding antioxidant and cytotoxicity abilities, possibly due to its richness in flavonoids class, particularly isoflavones. In turn, F1 was characterized by the presence of the most polar compounds from MeOH-E (organic acids and piscidic acid) and showed promising abilities to inhibit α-amylase, while F4, which contained prenylated flavonoids and furanoflavonoids, was the most active against the tested bacteria. The gathered results emphasize the distinct biological potentials of purified fractions of Retama raetam.

scholarly journals STUDIES ON BIOSURFACTANT PRODUCED USING Exiguobacterium profundum

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Nur Asni Setiani ◽  
Welly Octaviyani ◽  
Syarif Hamdani ◽  
Irma Mardiah
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Pharmaceutical Preparations ◽  
Initial Screening ◽  
Gram Negative ◽  
Emulsification Index ◽  
Exiguobacterium Profundum ◽  
Layer Chromatography

Background: The manufacture of pharmaceutical preparations generally adds surfactants. Microbial biosurfactants can be an alternative because biodegradable and have antibacterial properties.   Objective: This study aimed to examine the biosurfactant activity of Exiguobacterium profundum.Methods: Hemolysis and spreading oil tests were performed as an initial screening. Biosurfactant production was carried out by growing bacteria on oil-enriched media with shaker system for 7 days. Biosurfactant activity can be seen from the emulsification index, while the characterization of biosurfactant were used thin layer chromatography and antibacterial qualitative testing.Results: Exiguobacterium profundum could spread the oil layer and form micelles. The emulsification index on days 0, 1, 3, 5, and 7 showed percentage in sequence 44.83%, 48.28%, 48.28%, 40%, and 43.75%. The result of TLC showed lipopeptide group which is marked with red stain with ninhydrin appearance. Antibacterial testing using Escherichia coli showed the formation of clear zones around the disk paper.Conclusion: The biosurfactant produced by Exigoubacterium profundum can be classified into lipopeptide group which has antibacterial activity against gram-negative.

A very rapid in situ Kikuchi technique to determine relative crystal misorientation

1988 ◽  
Vol 46 ◽  
pp. 830-831
Author(s):  
J. I. Bennetch
Keyword(s):  
Electron Beam ◽  
Analytical Techniques ◽  
Superplastic Forming ◽  
The Other ◽  
Kikuchi Pattern ◽  
Kikuchi Line ◽  
Line Analysis

In a recent study of the superplastic forming (SPF) behavior of certain Al-Li-X alloys, the relative misorientation between adjacent (sub)grains proved to be an important parameter. It is well established that the most accurate way to determine misorientation across boundaries is by Kikuchi line analysis. However, the SPF study required the characterization of a large number of (sub)grains in each sample to be statistically meaningful, a very time-consuming task even for comparatively rapid Kikuchi analytical techniques.In order to circumvent this problem, an alternate, even more rapid in-situ Kikuchi technique was devised, eliminating the need for the developing of negatives and any subsequent measurements on photographic plates. All that is required is a double tilt low backlash goniometer capable of tilting ± 45° in one axis and ± 30° in the other axis. The procedure is as follows. While viewing the microscope screen, one merely tilts the specimen until a standard recognizable reference Kikuchi pattern is centered, making sure, at the same time, that the focused electron beam remains on the (sub)grain in question.

Rapid Isolation of High Molecular Weight Urokinase from Native Human Urine

1982 ◽  
Vol 47 (03) ◽  
pp. 197-202 ◽  
Author(s):  
Kurt Huber ◽  
Johannes Kirchheimer ◽  
Bernd R Binder
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Human Urine ◽  
Gel Filtration ◽  
Chain Structure ◽  
The Other ◽  
Single Chain ◽  
Apparent Homogeneity ◽  
Sequential Adsorption

SummaryUrokinase (UK) could be purified to apparent homogeneity starting from crude urine by sequential adsorption and elution of the enzyme to gelatine-Sepharose and agmatine-Sepharose followed by gel filtration on Sephadex G-150. The purified product exhibited characteristics of the high molecular weight urokinase (HMW-UK) but did contain two distinct entities, one of which exhibited a two chain structure as reported for the HMW-UK while the other one exhibited an apparent single chain structure. The purification described is rapid and simple and results in an enzyme with probably no major alterations. Yields are high enough to obtain purified enzymes for characterization of UK from individual donors.

Pharmacognostic studies of Morinda brevipes S. Y. Hu

2019 ◽  
Vol 48 (3) ◽  
pp. 401-407
Author(s):  
Rong Li ◽  
Xiaojing Lin ◽  
Genqiu Tang ◽  
Junni Li ◽  
Dong Wang ◽  
...  
Keyword(s):  
Calcium Oxalate ◽  
Transverse Section ◽  
Volatile Oils ◽  
Needle Crystal ◽  
Cork Cell ◽  
Registration Number ◽  
Phytochemical Studies ◽  
First Time ◽  
Layer Chromatography ◽  
Inequality Type

DNA barcoding of ITS and psbA-trnH regions, histochemistry as well as thin layer chromatography (TLC) of Morinda brevipes S.Y. Hu were analyzed. Transverse section of root revealed the presence of cortex, xylem, cork cell, stone cells, and calcium oxalate sandy crystal. The lower epidermis cells showed many stoma in paracytic or inequality type. Spiral vessel and tiny calcium oxalate needle crystal usually appeared in the powder. TLC showed the presence of emodin in M. brevipes. Phytochemical studies revealed the existence of carbohydrates, saponins, tannins, flavones, anthraquinones, alkaloids and volatile oils. The ITS and psbAtrnH sequences were found for the first time which were submitted to NCBI to obtain the GenBank registration number. This study might play an important role in the identification, and utilization of M. brevipes for various purposes.

Extraction, isolation and characterization of Bauhinia variegata flower

2020 ◽  
pp. 9-12
Author(s):  
Akanksha Gupta ◽  
Abhishek K Tripathi ◽  
Pushpraj S Gupta
Keyword(s):  
Biological Activities ◽  
Molecular Formula ◽  
Native Plant ◽  
Compound A ◽  
Active Constituents ◽  
Chromatographic Techniques ◽  
Isolation And Characterization ◽  
Soxhlet Apparatus ◽  
Bauhinia Variegata

Background: Bauhinia variegata Linn. is a native plant of Asia and China. B. variegata is found in tropical regions of the world. It belongs to family Leguminosae. It is used for diarrhea, hemorrhoids, constipation, piles, edema, leprosy, wounds, tumors, etc.  Objective: The objective of the present study was to perform extraction of B. variegata flower and isolation of active constituents from the extract. Materials and Methods: The ethanolic extraction of B. variegata flower was performed using the Soxhlet apparatus. The isolation of active constituents from the extract was performed using chromatographic techniques. In column chromatographic studies, n-hexane- [dichloromethane (DCM)] (2:8) was used as an eluting system and further purified through thin layer chromatography (TLC). Compound A and B were isolated through chromatographic techniques, then the molecular formula and characterization of these compounds were carried out with mass and infrared (IR) spectral analysis. Results and Discussion: The percentage yield of B. variegata ethanolic extract (BVE) was found to be 20.8% w/w. The different fractions were F1 having 12.5 grams with n-hexane, F2 (17.1 grams) with CH2Cl2, F3 (21.2 grams) with EtOAc, and F4 (13.4 grams) with EtOH. Compound A and B were isolated from the solvent fractions of n-hexane-DCM (2:8) and EtOAc-DCM (1:9), respectively. The compound A was characterized as 3-hydroxy-6-methoxy-2-phenyl-4H-chromen-4-one. The compound B was characterized as 3-hydroxy-6-methyl-2-phenyl-4H-chromen-4-one. Conclusion: Thus, B. variegata flowers possess active components that need to identify their biological activities.

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