scholarly journals SCREENING OF PHYTOCHEMICALS AND QUANTITATIVE ESTIMATION OF TOTAL FLAVONOIDS AND PHENOLIC COMPOUNDS OF LAGASCEA MOLLIS CAV. (ASTERACEAE)

2014 ◽  
Vol 1 (2) ◽  
pp. 94-97
Author(s):  
Saradha M ◽  
Paulsamy S ◽  
Abinaya G
Keyword(s):  
Phenolic Compounds ◽  
Methanol Extract ◽  
Quantitative Estimation ◽  
Root Extract ◽  
Total Flavonoids ◽  
Phytochemical Screening ◽  
Plant Parts ◽  
Soxhlet Apparatus ◽  
Phytochemical Constituents ◽  
Leaves And Roots

Aim of the study was to analyze the phytochemical constituents and estimation of total flavonoids and phenolic compounds of leaves and roots of the medicinal plant Lagascea mollis. Methanol extract of dried leaves and roots of L. mollis was prepared by using soxhlet apparatus. The extract prepared was tested forpreliminary qualitative phytochemical screening, followed by the quantitative estimation of total flavonoids and phenols by spectroscopy. The selected plant parts were found to contain alkaloids, flavonoids and terpenoids in both parts and saponin is present only in leaf. In quantitative estimation the reports revealedthe presence 0.017 and 0.013 mgRE/g extract and 458.91 and 704.50 mgGAE/g extract total flavonoids and phenolic compounds in leaf and root extract respectively. These major phytoconstituents present in this species may be accounted as factors for the medicinal importance of L. mollis.

Phytochemical Screening, Antimicrobial and Antioxidant Activities of Selected Mangrove Species

2020 ◽  
Vol 16 (2) ◽  
pp. 152-163 ◽  
Author(s):  
Nilesh L. Dahibhate ◽  
Utpal Roy ◽  
Kundan Kumar
Keyword(s):  
Staphylococcus Epidermidis ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Avicennia Marina ◽  
Total Flavonoids ◽  
Phytochemical Screening ◽  
Mangrove Species ◽  
Sonneratia Caseolaris ◽  
Bruguiera Gymnorhiza

Background: Mangrove plant extracts are used in folkloric medicine as aphrodisiac, sedative, antioxidant, antimicrobial and antimalarial. Screening for antimicrobial, phytochemical and antioxidant activities of fourteen mangroves plant species (Aegiceras corniculatum, Acanthus ilicifolius, Avicennia alba, Avicennia marina, Avicennia officinalis, Bruguiera cylindrica, Bruguiera gymnorhiza, Ceriops tagel, Excoecaria agallocha, Kandelia candel, Rhizophora apiculata, Rhizophora mucronata, Sonneratia alba, and Sonneratia caseolaris) in various solvents are reported in this work. Methods: The antimicrobial screening was carried out using agar well diffusion method. In this study, nine pathogenic strains were used, including three Gram-positive and six Gram-negative bacteria. Phytochemical screening, total flavonoids, total phenolic and antioxidant activity was tested by DPPH radical scavenging assay. Four phenolic compounds (gallic acid, vanillin, tannic acid and quercetin) were quantified by LC-MS/MS in selected mangrove species. Results: Antimicrobial screening showed Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis and Pseudomonas aeruginosa were most susceptible to ethyl acetate extract as compared to ethanol and methanol extract. Ethyl acetate extract of Avicennia marina and Bruguiera gymnorhiza showed strong antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis and Pseudomonas aeruginosa. Phytochemical analysis revealed the presence of saponins, phenolics, flavonoids, alkaloids, tannins, and terpenoids, which was found to be variable as per the solvent used for extraction. In addition, total phenolics and total flavonoids content with different solvents were found in the range of 11.08 to 196.76 mg GAE/g and 12.92 to 110.3 mg QE/g of extract respectively. Moreover, antioxidant capacities expressed in terms of IC50 (mg/mL) showed that methanol extract exhibited higher antioxidant capacity followed by ethanol extract. LC-MS/MS analysis showed gallic acid and tannic acid are present in higher concentration in Aegiceras corniculatum and Sonneratia caseolaris than other species. The vanillin and quercetin were found in the least concentration. Conclusion: Mangroves species are rich source of antioxidant, phenolics and antimicrobial compounds.

scholarly journals Phytochemical screening and hemolytique activity of some leaves extracts of Lantana camara L.

2021 ◽  
Vol 8 (3) ◽  
pp. 1-9
Author(s):  
Fahima Talhi ◽  
Noureddine Gherraf ◽  
Amar Zellagui ◽  
Awatif Boumaza ◽  
Amira Meghlaoui
Keyword(s):  
Phenolic Compounds ◽  
Hemolytic Activity ◽  
Methanolic Extract ◽  
Petroleum Ether Extract ◽  
Lantana Camara ◽  
Ether Extract ◽  
Phytochemical Screening ◽  
Long Time ◽  
Therapeutic Properties ◽  
Phytochemical Constituents

Abstract Medicinal plants have several therapeutic properties; they have been used for a long time to treat different diseases. Lantana camara L. has been widely used by man for healing these diseases. In this study, four leaves extracts of L. camara were subjected to preliminary phytochemical screening to determine the presence and/or the absence of phytochemical constituents; In addition, they were tested for hemolytic activity on human erythrocytes. This activity is performed using the UV-Vis spectrophotometer method at 520 nm and at five different concentrations (125 µg/ml, 250 µg/ml, 500 µg/ml, 750 µg/ml, and 1000 µg/ml). The phytochemical screening showed the presence of various phytochemical groups such as phenolic compounds, saponins, sterols, tannins, flavonoids, reducing compounds and the absence of alkaloids in the four extracts. These same extracts showed average hemolytic activity sequentially: chloroformic extract, petroleum ether extract, aqueous extract and then methanolic extract. This activity is dependent on the concentration of the extract.

scholarly journals Phytochemical Screening and Antibacterial Activity of Stem Bark, Leaf and Root Extract of Sclerocarya birrea (A. Rich.) Hochst

2017 ◽  
Vol 5 (4) ◽  
pp. 127-131
Author(s):  
Lawaly Maman Manzo ◽  
Halima Diallo Bako ◽  
Moussa Idrissa
Keyword(s):  
Antibacterial Activity ◽  
Gastrointestinal Diseases ◽  
Stem Bark ◽  
Root Extract ◽  
Root Bark ◽  
Phytochemical Screening ◽  
Zone Of Inhibition ◽  
Traditional Use ◽  
Plant Parts ◽  
Sclerocarya Birrea

Background: Sclerocarya birrea is widely used in Nigerien communities for medicinal purposes to treat several gastrointestinal diseases including diarrhea. To lend credibility to its traditional use against diarrhea, laboratory studies were conducted. Objective: The aim of this study is to investigate the antibacterial activity and the phytochemical constituents of the crude extracts of root, bark and leaf of S. birrea. Materials and Methods: The collected different plant parts were air dried, powdered and separately extracted with ethanol and methanol. The alkaloid, flavonoid, saponin and tannin contents in all the plant parts were estimated using standard methods. The total and serially diluted fractions of the extracts were tested for antibacterial activity against selected enteropathogens by agar well diffusion and deep-well microdilution method. Results: Phytochemical screening revealed the presence of flavonoid, saponin and tannin in all the plant extracts. The extracts from the different parts showed varied antibacterial activity against the test bacteria. The bark extracts showed superior activity against Escherichia coli (zone of inhibition = 17 mm) and Salmonella typhi (zone of inhibition = 20 mm) at 200 mg/mL. Conclusion: The presence of important phytochemical groups and the antibacterial potential of alcoholic extracts of S. birrea could permit to justify its traditional usage against diarrhea.

Phytochemical Study and Antioxidant Activities on Extracts of the Leaves and Roots of Costus afer Ker Gawl. (Zingiberaceae)

2020 ◽  
Vol 36 (05) ◽  
pp. 804-811
Author(s):  
Coulibaly Wacothon Karime ◽  
Adiko N’dri Marcelline ◽  
Benie Anoubilé ◽  
Konan Yao Mokey Jean-Baptiste ◽  
Bea Gouanda Thibaut ◽  
...  
Keyword(s):  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Phytochemical Screening ◽  
Phytochemical Study ◽  
Average Value ◽  
Dpph Method ◽  
Phytochemical Constituents ◽  
Leaves And Roots ◽  
Layer Chromatography ◽  
Costus Afer

The extracts of leaves and roots of Costus afer plant have been fully investigated in an attempt to determine their phytochemical constituents and antioxidant activities. Phytochemical screening carried out using thin layer chromatography (TLC) revealed the presence of several secondary metabolites in all the selected extracts of the plant. In addition, our results showed that the leaves of C. afer are the richest in polyphenols with an average value of 3416.25 μg EAG/g MS. The total flavonoid assay revealed a highest content in the leaves (8.02 %). Furthermore, studies of the antioxidant activities using 2,2-diphenyl-1-picrylhydrazyl (DPPH•) method showed a significant effect compared to the reference vitamin C. Ethyl acetate extract of the leaves show a higher percentage of inhibition (83 %), followed the roots (69 %).

scholarly journals HPLC Analysis and Antimicrobial Screening of Methanol Extract/Fractions of the Root of Millettia aboensis (Hook.f.) Baker against Streptococcus mutans

2020 ◽  
pp. 1-11
Author(s):  
Adeniran J. Ikuesan ◽  
Eze E. Ajaegbu ◽  
Ukachukwu C. Ezeh ◽  
Adaobi J. Dieke ◽  
Adaora L. Onuora ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Methanol Extract ◽  
Extraction Methods ◽  
Antimicrobial Activities ◽  
Root Extract ◽  
Plant Materials ◽  
Aqueous Fraction ◽  
Plant Parts ◽  
Percentage Yield ◽  
Phytochemical Compounds

Millettia aboensis (Hook.f.) Baker belongs to the Leguminosae family, known locally as nduezi in Igbo, erurumesi in Edo, and Òdúdū in Efik. The plant parts have proven to be active against some diseases. The plant was studied for its antimicrobial activity and the phytochemicals present. The plant materials were macerated and fractionated using different extraction methods. The compounds present in the fractions of the stem were detected with the aid of HPLC-DAD. The efficacy of the crude methanol and fractions from the root part was evaluated against Streptococcus mutans. The results showed that the aqueous fraction extract had the highest percentage yield (51.11%), followed by the ethyl acetate (24.10%), butanol (6.63%), and hexane (5.56%), while the methanol extract had a yield of 2.48%. Nine phytochemical compounds were detected from the fractions of the root extract: bis-oxazolidinone derivative – 1, circumdatin F – 2, enniatin B – 3, septicine – 4, orobol 8-C-glucoside – 5, rocaglamide – 6, genistein 6-C-glucoside – 7, 3-phenyl chromen-4-one – 8, and corynesidone D – 9. The methanol extract/fractions of M. aboensis showed no antimicrobial activities on clinical isolates at different concentrations (6.25-50 mg/ml). Based on the poor activity against Strep. mutans, but with promising phytoconstituents present, the extract/fractions need further investigation as regards its utilization against other pathogens.

scholarly journals Extraction of Phytochemical Constituents from the root of Eclipta Alba through Gas chromatography-mass spectrometry procedure

2019 ◽  
Vol 10 (2) ◽  
pp. 1238-1242
Author(s):  
Satheesh Naik K ◽  
Gurushanthaiah M ◽  
Kavimani M ◽  
Mahesh GM
Keyword(s):  
Gas Chromatography ◽  
Methanol Extract ◽  
Folk Medicine ◽  
Gas Chromatography Mass Spectrometry ◽  
Root Extract ◽  
Dodecanoic Acid ◽  
Eclipta Alba ◽  
Wide Range ◽  
Phytochemical Constituents ◽  
Gas Chromatography Mass Spectroscopy

The higher herbal values of Eclipta Alba have a long history in the treatment of various ailments in traditional and folk medicine in many tropical and sub-tropical countries. Medicinal plants play a significant role in human culture and civilization. Eclipta Alba plant roots were powdered. By using simple soxhlation method, methanol extract was prepared. The powdered extract of Eclipta alba and methanol extract was concentrated and analyzed using Gas Chromatography-Mass Spectroscopy to identify the biochemical components of Eclipta Alba root extract in the present study. A wide range of active ingredients such as 2- Thiophene carbaldehyde, 5-[5-(thien-2-yl) thien-2-yl]- 0.08%, Dodecanoic acid - 0.19, 9-Octadecenamide, (Z)- (CAS) OLEOAMIDE - 0.29, Loliolide – 0.37 and– 44.86. This study supports, methanolic extract of eclipta alba has potential antimicrobial, antioxidant and hepatoprotective activities.

scholarly journals Phytochemical Screening of Medicinal Plants and Study of the Effect of Phytoconstituents in Seed Germination

2020 ◽  
Vol 35 (2) ◽  
pp. 1-11
Author(s):  
Tinky Sharma ◽  
Binjita Pandey ◽  
Bishnu Kumar Shrestha ◽  
Gayatri Maiya Koju ◽  
Rojeena Thusa ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Seed Germination ◽  
Medicinal Plants ◽  
Pisum Sativum ◽  
Cytotoxic Effect ◽  
Methanol Extract ◽  
Living Cells ◽  
Phytochemical Screening ◽  
Volatile Oils ◽  
Plant Parts

 Phytochemical screening of ten different locally available plant parts was done in methanol extract. Tannin, quinine, terpenoid, flavonoid, steroid, alkaloid, cardiac glycoside, glycoside, volatile oils, etc were the phytoconstituents found in plants. The study of the effect of phytoconstituents in the germination of Pisum sativum seeds revealed that the phytoconstituents present in plant extracts showed the cytotoxic effect in living cells i.e. in germinating Pisum sativum seeds. The phytoconstituents present in the plant extract showed an effect on cell proliferation and growth. Hence these plants could be used to develop drugs against cancer cells and also may be effective against microbes and bacteria

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND GC-MS ANALYSIS OF METHANOLIC LEAF EXTRACT OF DRYPETES SEPIARIA (WIGHT & ARN.) PAX. & HOFFIM. FROM SILAMBUR SACRED GROVE, TAMILNADU

2017 ◽  
Vol 4 (2) ◽  
pp. 157-161
Author(s):  
Saravanakumar K
Keyword(s):  
Extraction Method ◽  
Methanol Extract ◽  
Soxhlet Extraction ◽  
Sacred Grove ◽  
Phytochemical Screening ◽  
Plant Materials ◽  
Ms Analysis ◽  
Successive Extraction ◽  
Phytochemical Compounds ◽  
Phytochemical Constituents

Drypetes sepiaria (Wight & Arn.) Pax. & Hoffim a medium sized tree member of Euphorbiaceae was investigated to determine the phytochemical constituents present in various extracts of the leaves through GC-MS analysis. Powdered leaf plant materials were subjected to successive extraction with organic solventssuch as methanol by Soxhlet extraction method. In the present study, GC-MS analysis revealed that a total of 23 different compounds identified by using methanol extract and all the identified compounds were medicinally valuable for the treatment of various human ailments. In addition, all the phytochemical compounds were needed for further investigations on toxicological aspects for the development of new lead of therapeutic interest.

scholarly journals PHYTOCHEMICAL SCREENING OF SELECTED SPECIES FROM CONVOLVULACEAE

2017 ◽  
Vol 9 (6) ◽  
pp. 94 ◽  
Author(s):  
Maria Emilia Mascarenhas ◽  
Cibani Ramesh Mandrekar ◽  
Pratiksha Bharat Marathe ◽  
Luena Joey Morais
Keyword(s):  
Phenolic Compounds ◽  
Methanol Extract ◽  
Phytochemical Screening ◽  
Preliminary Screening ◽  
Stems And Leaves

Objective: To screen phytochemicals from stems and leaves of 23 selected taxa of Convolvulaceae.Methods: Stem and leaves of the selected 23 taxa were collected and shade dried. Methanol extract was used for preliminary screening of phytochemicals such as alkaloids, carbohydrates, glycosides, saponins, proteins, phytosterols, tepenoids, fixed oils, phenolic compounds, flavonoids and tannins.Results: Phytochemicals were present in all selected taxa of Convolvulaceae. Leaves showed most of the phytochemicals than stems. The important phytochemicals in leaves were flavonoids, carbohydrates, alkaloids, saponins, tannins and phenolic compounds while those in stems were carbohydrates, saponins and phenolic compounds. Proteins and fixed oils were absent in the taxa studied.Conclusion: Phytochemical screening in the present study, revealed that maximum phytochemicals were present in leaves than in stems.

scholarly journals Central Nervous System Activity Studies of Baptisia tinctoria (L.) R. Vent. Roots

2017 ◽  
Vol 6 ◽  
pp. 1-7
Author(s):  
Sujata ◽  
Deepak Kumar ◽  
Suresh Kumar
Keyword(s):  
Central Nervous System ◽  
Methanol Extract ◽  
Ethyl Acetate Fraction ◽  
Acute Administration ◽  
Phytochemical Screening ◽  
Standard Drug ◽  
Nervous System Activity ◽  
Central Nervous System Activity ◽  
Soxhlet Apparatus ◽  
Analgesic Activities

The present investigations were undertaken with a view to evaluate Baptisia tinctoria roots (Wild Indigo; family – Fabaceae) systematically for neuropharmacological activities. The methanol extract (ME) of plant was prepared by extracting properly identified plant in a Soxhlet apparatus with methanol, after defatting with n-hexane. Acute toxicity studies revealed that ME is safe for acute administration. Preliminary phytochemical screening of ME showed presence of alkaloids, steroids, flavonoids, triterpenoids, coumarins and tannins as major classes of phytoconstituents. The ethyl acetate fraction (EAF) was prepared by fractionating crude ME using standardized procedure, which showed presence of flavonoids, alkaloids and triterpenoids. ME (400 mg/kg) and EAF (106 mg/kg) produced significant antistress activity in similar manner as exhibited by the standard drug. The ME and EAF exhibited mild antianxiety activity, and were found to be devoid of anticonvulsant, sedative and analgesic activities.

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