scholarly journals PHYTOCHEMICAL INVESTIGATION BY USING TENDER LEAF PART OF GREWIA TILIIFOLIA VAHL.

2017 ◽  
Vol 4 (3) ◽  
pp. 68-70
Author(s):  
Renjini Haridas ◽  
Sumathi P ◽  
Binu Thomas
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Secondary Metabolite ◽  
Qualitative And Quantitative ◽  
Solvent Extract ◽  
Phytochemical Investigation ◽  
Potential Activity ◽  
Phytochemical Constituents ◽  
Leaf Part ◽  
Tender Leaf

This study was aimed to evaluate the phytochemical potential of different extracts of tender leaf part of Grewia tiliifolia Vahl which are commonly used in Ayurveda drug preparations. Tender leaf part of G.tiliifolia subjected to analyze the phytochemical constituents by using qualitative and quantitative methods.The results of the present investigation revealed that the presence of flavonoid, phenol, tannin glycoside, resin, steroids, terpenoids and triterpenoids in different solvent extract like petroleum ether, chloroform, ethyl acetate, methanol and water. Tender leaves of the species which exhibited well marked potential activity and rich in secondary metabolite contents (flavonoids and phenols).

scholarly journals Extraction of Phytochemical Compounds of Leea guineensis (G. Don) Leaves Using Non-polar and Polar Solvents

2020 ◽  
pp. 24-31 ◽  
Author(s):  
O. L. Awotedu ◽  
U. E. Okeke ◽  
P. O. Ogunbamowo ◽  
O. S Ariwoola ◽  
T. O. Omolola
Keyword(s):  
Ethyl Acetate ◽  
Cardiac Glycosides ◽  
Polar Solvent ◽  
Chemical Components ◽  
Polar Solvents ◽  
Qualitative And Quantitative ◽  
Solvent Extract ◽  
Phytochemical Compounds ◽  
Phytochemical Constituents ◽  
Selection Of

Aims: Selection of a suitable solvent is important and utilized in the extraction of desirable chemical components in medicinal plants. Study Design: Chemical analysis of various extracts of Leea guineensis leaves using standard analytical procedures. Place and Duration of Study: Forestry Research Institute of Nigeria, between March 2019 and August 2019. Methodology: Leaves of Leea guineensis were extracted with six solvents categorized into polar (Acetone, Methanol and Aqueous) and non-polar (Ethyl acetate, Hexane and Chloroform) types using cold maceration method, the qualitative and quantitative phytochemical assay was done on the respective extracts using the standard methods. Results: Phytochemical screening of the non-polar solvent extract revealed the presence of flavonoids, alkaloids and saponins for all the solvents, while tannin was present only in ethyl acetate extract. For the polar solvent extracts, all the secondary metabolites determined were present except terpenoids and phlobatanins. In the quantitative test, alkaloid levels ranged from 1.31-38.25 mg/100 g, saponin: 2.01-14.35 mg/100 g, flavonoids: 1.10-6.25 mg/100 g, Tannin: ND-4.62 mg/100 g, terpenoids: ND-1.02 mg/100 g, cardiac glycosides: ND-0.84 mg/100 g, Anthraquinone: ND-2.58 mg/100 g and phlobatanins: ND-0.95. The results obtained for each of the phytochemicals are significantly different (p<0.05) across all the solvent extracts, while phytochemicals such as terpenoids, cardiac glycosides, anthraquinones and phlobatanins were not detected in the non-polar solvent extract of L. guineensis. Conclusion: The phytochemical constituents detected in varying quantities depend on the polarity of the substances, L. guineensis could be exploited and extracted very well using a polar solvent like methanol, acetone and aqueous.

scholarly journals PRELIMINARY PHYTOCHEMICAL STUDIES OF SELECTED MEDICINAL PLANT PACHYGONE OVATA (POER.) HOOK.F. & THOMS FROM MENISPERMACEAE FAMILY FOR BIOACTIVE CONSTITUENTS

2018 ◽  
Vol 5 (2) ◽  
pp. 50-52
Author(s):  
Renjini Haridas ◽  
Radhakrishnan G ◽  
Reshma R ◽  
Sumathi P
Keyword(s):  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Cardiac Glycosides ◽  
Ethanolic Extract ◽  
Phytochemical Analysis ◽  
Bioactive Constituents ◽  
Phytochemical Studies ◽  
Leaf Part ◽  
Important Medicinal Plant

The present study deals with the phytochemical examination of Pachygone ovata (Poer.) Hook.f.& Thoms., an important medicinal plant from menispermaceae family. Leaf and Stem extracts were prepared by using different solvents systems and phytochemical screening was performed using the standard methods given by Harborne. Leaf and stem extracts were prepared from aqueous and organic solvents like petroleum ether, acetone, ethyl acetate and ethanol. Qualitative phytochemical analysis of the petroleum ether, acetone,ethyl acetate, ethanol and aqueous extracts prepared from P. ovata leaf and stem part. Leaf part revealed the presence of alkaloids, flavonoids, glycosides, cardiac glycosides, phenols and tannins. Stem part revealed the presence of alkaloids, flavonoids, glycosides, Resin, Steroids, phenols and tannins. The ethanolic extract showed higher amount of secondary metabolites than the other solvent extracts. This observation becomes important in the context of the therapeutically and drug applications of P. ovata.

scholarly journals QUALITATIVE PHYTOCHEMICAL COMPARISON BETWEEN FLAVONOIDS AND PHENOLIC ACIDS CONTENTS OF LEAVES AND FRUITS OF MELIA AZEDARACH (FAMILY: MELIACEAE) CULTIVATED IN IRAQ BY HPLC AND HPTLC

2016 ◽  
Vol 8 (10) ◽  
pp. 242 ◽  
Author(s):  
Noor S. Jaafar ◽  
Maha N. Hamad ◽  
Ibrahim S. Abbas ◽  
Iman S. Jaafar
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Phenolic Acids ◽  
Melia Azedarach ◽  
Butanol Fraction ◽  
Phytochemical Investigation ◽  
Dried Extract

<p><strong>Objective: </strong>The aim of our study was to compare between flavonoids and phenolic acids contents of leaves and fruits of <em>Melia azedarach</em> since no phytochemical investigation had done previously in Iraq.</p><p><strong>Methods: </strong>The leaves and fruits of <em>Melia azedarach </em>were extracted by soxhlet using 80% ethanol then the dried extract was suspended in water and fractionated using petroleum ether, chloroform, ethyl acetate, and n-butanol. The n-butanol fraction was hydrolyzed by acid and partitioned with ethyl acetate. The different fractions containing flavonoids and phenolic acids were analyzed by HPLC and HPTLC.</p><p><strong>Results: </strong>The HPLC results revealed the presence catechin-7-O-glycoside in fruit only, while kaempferol-7-O-glycoside is found in the leaves only. Catechin and its glycosides are more abundant in the fruits than in the leaves. The HPTLC results revealed that kaempferol and quercetin are present in all fractions of leaves and fruits as aglycones and as glycosides. Free chlorogenic was found in both leaves and fruits<strong>.</strong></p><p><strong>Conclusion: </strong>No major differences were found between the flavonoids and phenolic acids contents of the leaves and fruits of <em>Melia azedarach.</em></p>

scholarly journals ANTICONVULSANT AND SEDATIVE ACTIVITIES OF EXTRACTS OF CARISSA CARANDAS LEAVES.

2018 ◽  
Vol 8 (5) ◽  
pp. 369-373 ◽  
Author(s):  
Manisha Shinde ◽  
Ritu Gilhotra ◽  
Sanjay Chaudhari
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Sodium Pentobarbital ◽  
Standard Drug ◽  
Crude Extracts ◽  
Phytochemical Investigation ◽  
Carissa Carandas ◽  
Ethanol Extracts

The present study was carried out to find out the preliminary phytochemical properties, anticonvulsant and sedative activities of the petroleum ether, ethyl acetate and ethanol extract of Carissa carandas leaves. The standard methods were used to screen the preliminary photochemicals present  in petroleum ether, ethyl acetate and ethanol extracts of Carissa carandas leaves. The anticonvulsant efficacy of the extracts was determined using pentylenetetrazole (PTZ) in experimental animal models using diazepam as standard drug and the sedative effect was evaluated using pentobarbitone that induced sleep in mice. The efficacy of the extracts was compared against the standard drug Diazepam .The preliminary phytochemical investigation shows presence of alkaloids, glycoside, tannins,terpins . The crude extracts of ethanolic, ethyl acetate and petroleum ether at a dose 400 mg/kg has been found to significantly reduce the extensor and stupor and offer protection against convulsion induced by PTZ. The petroleum ether, ethyl acetate and ethanolic extract found to be non significant at a dose of 100 mg/kg in flexion. It is seen that the crude extracts of ethanolic, ethyl acetate and petroleum ether Of Carissa carandas leaves significantly (p < 0.05) prolonged the time of sodium pentobarbital-induced hypnosis. However, the animal study revealed that the anticonvulsant and sedative activities of Carissa carandas can be used in the treatment of epilepsy. Key words- Carissa carandas, anticonvulsant, pentobarbitone, hypnosis, epilepsy.          

scholarly journals PHYTOCHEMICAL ANALYSIS OF LEAF, STEM AND ROOT IN EUPHORBIA ROTHIANA SPRENG. (EUPHORBIACEAE), THE NILGIRIS WESTERN GHATS, TAMIL NADU

2017 ◽  
Vol 4 (3) ◽  
pp. 17-18
Author(s):  
Anusuya Devi R ◽  
Veena B ◽  
Thenmozhi K
Keyword(s):  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Western Ghats ◽  
Tamil Nadu ◽  
Phytochemical Analysis ◽  
Phytochemical Constituents ◽  
Ethanol Extracts ◽  
The Nilgiris ◽  
Important Medicinal Plant

Euphorbia rothiana Spreng. is an important medicinal plant. It used in hypertensive agent in traditional medicine.The present study deals with the analysis of Phytochemical constituents by qualitative analysis of leaves, stem and root were done using Petroleum ether, chloroform, ethyl acetate and ethanol extracts. Alkaloids, flavonoids, phenols, terpenoids, triterpinoids, steroids, cardio glycosides and carbohydrates were analysed. Alkaloids, flavonoids, and phenols were highly present various extracts of leaves stem and root. Cardio glycosides triterpinoids and carbohydrates were minimum present in the various extracts.

scholarly journals PHYTOCHEMICAL INVESTIGATION OF LEAVES AND SEEDS OF CORCHORUS OLITORIUS L. CULTIVATED IN IRAQ

2018 ◽  
Vol 11 (11) ◽  
pp. 408 ◽  
Author(s):  
Hayder T Hasan ◽  
Enas J Kadhim
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Phenolic Acid ◽  
High Performance ◽  
Corchorus Olitorius ◽  
Octadecanoic Acid ◽  
Plant Materials ◽  
Petroleum Ether Fraction ◽  
Phytochemical Investigation ◽  
Ether Fraction

Objective: The aim of this study was to investigate chemical constituents of leaves and seeds of Corchorus olitorius since no phytochemical investigation had been done previously in Iraq.Methods: Leaves and seeds of C. olitorius were defatted by maceration in hexane for 24 h. The defatted plant materials were extracted using Soxhlet apparatus, the aqueous methanol 85% as a solvent extraction for 24 h, and fractionated by petroleum ether, chloroform, ethyl acetate, and n-butanol. The ethyl acetate, n-butanol, and n-butanol after hydrolysis fractions for each part (leaves and seeds) were analyzed by high-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) for its phenolic acid and flavonoid contents. The petroleum ether fraction from the leaves was analyzed using Gas chromatography/mass spectrometer (GC/MS). Flavonoids and phenolic acid derivative were isolated from the ethyl acetate of leaf fraction and n-butanol after hydrolysis fraction of the seeds and identified by mass spectrometry, infrared, HPLC, and HPTLC.Results: The different chromatographic and spectroscopic results revealed the presence of luteolin, quercetin, astragalin, isoquercetin, catechins, and 3,5-dicaffeoylquinic acid (3,5-DCQA) in both leaves and seeds of C. olitorius and also 17-octadecynoic acid, 9-octadecanoic acid (oleic acid), hexadecenoic acid (palmitic acid), 9,12-octadecanoic acid (linoleic acid), octadecadien-1-ol (β-sitosterol), and α-tocopherol (Vitamin E) in the petroleum ether fraction of C. olitorius leaves.Conclusion: The results of the current study proved the presence of 3,5-DCQA, astragalin, and isoquercetin in the ethyl acetate fraction of C. olitorius leaves and catechin in the n-butanol after hydrolysis fraction of C. olitorius seeds. 

scholarly journals Hplc analysis of phenolic acids in mountain germander (Teucrium montanum L) extracts

2004 ◽  
pp. 265-273 ◽  
Author(s):  
Vesna Tumbas ◽  
Anamarija Mandic ◽  
Gordana Cetkovic ◽  
Sonja Djilas ◽  
Jasna Canadanovic-Brunet
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Phenolic Acids ◽  
High Performance ◽  
Phenolic Content ◽  
Petroleum Ether Extract ◽  
Total Phenolic ◽  
Qualitative And Quantitative ◽  
Butanol Extract ◽  
Quantitative Content

The methanol, petroleum ether, chloroform, ethyl acetate, 1-butanol and water extracts were obtained by extraction of mountain germander (Teucrium montanum L). The total phenolic content in extracts was measured by Folin-Ciocalteu method. The 1-butanol extract had the highest phenolic content (296.00 mg/g). High performance liquid chromatography (HPLC) was employed to define qualitative and quantitative content of phenolic acids in mountain germander extracts. The largest number of phenolic acids were determined in ethyl acetate and 1-butanol extracts, while these acids were not present in petroleum ether extract. The highest content of phenolic acids (28.619 mg/g) had ethyl acetate extract and gentisic acid (14.432 mg/g) was its major component. Despite of a large number of phenolic acids in 1-butanol extract their content was only 3.740 mg/g.

scholarly journals PHYTOCHEMICAL INVESTIGATION AND QUANTITATIVE ESTIMATION OF FLAVONOID AND PHENOLIC CONTENTS OF THE ROOT, STEM AND LEAVES OF TEPHROSIA PURPUREA LINN

2018 ◽  
Vol 8 (5-s) ◽  
pp. 283-287 ◽  
Author(s):  
R Nigam ◽  
R Arnold
Keyword(s):  
Quantitative Estimation ◽  
Ethanolic Extract ◽  
Phytochemical Analysis ◽  
Herbal Drugs ◽  
Qualitative And Quantitative ◽  
Phenolic Contents ◽  
Tephrosia Purpurea ◽  
Phytochemical Investigation ◽  
Aqueous Ethanolic Extract ◽  
Phytochemical Constituents

Herbal drugs are traditionally used in various parts of the world to cure different diseases. The purpose of present study is to characterize phytoconstituents in the various part of Tephrosia purpurea. The root, stem and leaves of Tephrosia purpurea were washed, air dried and then powdered. The aqueous and ethanolic extracts of various part of Tephrosia purpurea were used for the phytochemical investigation to find out the qualitative and quantitative phytochemical constituents in the plant. The result of the phytochemical analysis of Tephrosia purpurea showed presence or absence in addition to quantitative (mg/100mg) contents of flavonoid and Phenol in the plant.Present study will help to identify the different parts of the plant from which higher quantities of the phytochemical can be derived and for the development of new herbal drugs from Tephrosia purpurea. Keywords: Phytoconstituents, Phytochemical, Tephrosia purpurea, Aqueous ethanolic extract, Flavonoid, Phenol.

Pharmacognostical, Phytochemical and Fluorescence analysis of the plant Bougainvillea spectabilis (Willd.)

2021 ◽  
pp. 3733-3738
Author(s):  
Prabhat Kumar Das ◽  
Jai Singh Vaghela ◽  
Narendra Badore
Keyword(s):  
Heavy Metal ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Fluorescence Analysis ◽  
Water Soluble ◽  
Leaf Extracts ◽  
Bioactive Natural Products ◽  
Leaf Powder ◽  
Bougainvillea Spectabilis ◽  
Phytochemical Constituents

Objective: The aim of the study is to investigate the Phytochemical, Pharmacognostical, Fluorescence analysis and heavy metal testing of the plant Bougainvillea spectabilis (WILLD.). Methods: The plant powder was extracted with different solvents such as petroleum ether, chloroform, ethyl acetate, ethanol, and water. The different extracts were tested qualitatively for the identification of various phytochemical constituents. The plant powder was subjected to fluorescence analysis in daylight and in ultraviolet-light (254 nm and 365 nm) and heavy metal testing. Results: Water soluble extractive value was found to be higher than ethanol, Ethyl acetate, chloroform and petroleum ether. The total ash values were found to be higher followed by water soluble ash and acid insoluble ash. From the phytochemical screening result showed the presence of various phytochemical constituents such as alkaloid, glycoside, Carbohydrate, Flavonoids, Saponins, Terpenoids, Taninns and phytosterols. Fluorescence analysis of leaf powder of Bougainvillea spectabilis showed characteristic coloration with various chemicals. The presence of heavy metals like cobalt, mercury, nickel, silver and zinc were found negative. Conclusion: Thus the bioactive natural products in leaf extracts of Bougainvillea spectabilis can be used in the development of new pharmaceuticals that enhances therapeutic use.

Phytochemical Constituents of Annona reticulata and their Cytotoxic Activity

2020 ◽  
Vol 17 (3) ◽  
pp. 206-210
Author(s):  
Ty Viet Pham ◽  
Thang Quoc Le ◽  
Anh Tuan Le ◽  
Hung Quoc Vo ◽  
Duc Viet Ho
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Structural Determination ◽  
Phytochemical Investigation ◽  
Ic50 Values ◽  
Phytochemical Constituents ◽  
First Time ◽  
Annona Reticulata

A phytochemical investigation of the leaves of Annona reticulata led to the isolation and structural determination of β-sitosterol (1), ent-pimara-8(14),15-dien-19-oic acid (2), ent-pimara- 8(14),15-dien-19-ol (3), quercetin (4), quercetin 3-O-α-L-arabinopyranoside (5), and a mixture of quercetin 3-O-β-D-galactopyranoside (6a) and quercetin 3-O-β-D-glucopyranoside (6b). Of these, compounds 2 and 3 were isolated from the genus Annona for the first time. Compound 3 showed strong cytotoxicity against SK-LU-1 and SW626 cell lines with IC50 values of 17.64 ± 1.07 and 19.79 ± 1.41 μg mL-1, respectively.

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