scholarly journals Hplc analysis of phenolic acids in mountain germander (Teucrium montanum L) extracts

2004 ◽  
pp. 265-273 ◽  
Author(s):  
Vesna Tumbas ◽  
Anamarija Mandic ◽  
Gordana Cetkovic ◽  
Sonja Djilas ◽  
Jasna Canadanovic-Brunet
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Phenolic Acids ◽  
High Performance ◽  
Phenolic Content ◽  
Petroleum Ether Extract ◽  
Total Phenolic ◽  
Qualitative And Quantitative ◽  
Butanol Extract ◽  
Quantitative Content

The methanol, petroleum ether, chloroform, ethyl acetate, 1-butanol and water extracts were obtained by extraction of mountain germander (Teucrium montanum L). The total phenolic content in extracts was measured by Folin-Ciocalteu method. The 1-butanol extract had the highest phenolic content (296.00 mg/g). High performance liquid chromatography (HPLC) was employed to define qualitative and quantitative content of phenolic acids in mountain germander extracts. The largest number of phenolic acids were determined in ethyl acetate and 1-butanol extracts, while these acids were not present in petroleum ether extract. The highest content of phenolic acids (28.619 mg/g) had ethyl acetate extract and gentisic acid (14.432 mg/g) was its major component. Despite of a large number of phenolic acids in 1-butanol extract their content was only 3.740 mg/g.

scholarly journals Phytochemical screening and antimicrobial activity of stem extract of Coleus forskohlii L. collected from Al-Baha Area, Saudi Arabia

2021 ◽  
Vol 16 (2) ◽  
pp. 078-086
Author(s):  
Abdulaziz Yahya Al-Ghamdi
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Petroleum Ether Extract ◽  
Ethanol Extract ◽  
Antimicrobial Activities ◽  
Chloroform Extract ◽  
Total Phenolic ◽  
Phytochemical Screening

This study aimed to investigate the phytochemical screening, total phenolic content, antioxidant and antimicrobial activities of Coleus forskohlii L. stem extract in Al-Baha area, Saudi Arabia. Stem samples were collected from Al-Baha area and air-dried followed by extraction with ethanol, petroleum ether, chloroform, ethyl acetate, and n-butanol. The extracts were then subjected to phytochemical screening, determination of total phenolic content, antioxidant and antimicrobial activities. Results showed the presence of flavonoids, alkaloids, tannins, terpenoids, steroids, saponins, and reducing sugars. Total phenolic content was significantly (P<0.001) higher in n-butanol extract (274.33±3.29 mg GAE/gm), followed by ethyl acetate extract (182.94±1.82 mg GAE/gm), ethanol extract (79.63±2.02 mg GAE/gm) and petroleum ether extract (73.38±3.07 mg GAE/gm), while the lowest content was in chloroform extract (60.06±2.12 mg GAE/gm). The antioxidant activity was significantly (P<0.001) higher in n-butanol extract (67.68±1.55%), followed by ethyl acetate extract (43.38±1.27%), ethanol extract (36.02±1.29%), petroleum ether extract (20.71±0.59%) and chloroform extract (19.73±0.74%). The antimicrobial activity showed that all microorganisms tested were resistant at the concentration of 25 and 50 mg/ml of plant extracts, whereas the concentrations of 100, 150 and 200 mg/ml showed varying activities against gram-negative (Escherichia coli, Pseudomonas aeruginosa), gram-positive (Staphylococcus aureus, Bacillus cereus) and Candida albicans. The study concluded that the stem extracts of C. forskohlli have promising pharmacological and biological activities that could be beneficial in pharmaceutical as well as food and medicinal industries.

Total phenolics and individual phenolic acids vary with light environment in Lindera benzoin

Botany ◽  
2010 ◽  
Vol 88 (11) ◽  
pp. 1007-1010 ◽  
Author(s):  
Christine M. Ingersoll ◽  
Richard A. Niesenbaum ◽  
Caitlin E. Weigle ◽  
Julia H. Lehman
Keyword(s):  
Phenolic Acids ◽  
High Performance ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Dihydroxybenzoic Acid ◽  
Lindera Benzoin ◽  
Array Detection ◽  
Sun And Shade ◽  
Ferulic Acids ◽  
Shade Plants

The understory shrub Lindera benzoin L. experiences lower rates of herbivory in sun environments than in shade environments. The production of secondary metabolites (e.g., phenolic compounds with known plant defense properties) is one likely contributor to these observed differences in herbivory. This work determined the total phenolic content as well as the concentrations of several individual phenolic acids in L. benzoin leaves found in sun and shade habitats. Total phenolic concentrations were determined to be higher in leaves from sun plants than in those from shade plants (47.5 ± 2.4 vs. 28.6 ± 1.3 gallic acid equivalents, respectively). High-performance liquid chromatography with diode array detection was used to separate and quantify several individual phenolic acids, and specific compounds were identified based on their retention times and ultraviolet spectra. The concentrations of vanillic, chlorogenic, p-coumaric, and ferulic acids were shown to be statistically higher in leaves from sun plants than in those from shade plants (P < 0.05), whereas 2,5-dihydroxybenzoic acid and caffeic acid were not significantly different in L. benzoin leaves from sun versus shade habitats.

scholarly journals Some phytochemical analyses of different extracts of the cogon grass Imperata cylindrica from Mizoram, India

2018 ◽  
Vol 18 (4) ◽  
pp. 120-124
Author(s):  
P.B. Lalthanpuii ◽  
Zarzokimi ◽  
K. Lalchhandama
Keyword(s):  
Petroleum Ether ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Petroleum Ether Extract ◽  
Chloroform Extract ◽  
Imperata Cylindrica ◽  
Total Phenolic ◽  
Ether Extract ◽  
Flavonoid Content ◽  
Chloroform Methanol

Cogon grass, Imperata cylindrica (L.) Räuschel, was studied for its flavonoid and phenol contents. A series of extracts of the underground (rhizome-root) parts was prepared by hot extraction using solvents of different polarities such as chloroform, methanol, and petroleum ether. The total flavonoid content of the plant extracts was determined based on the reaction with aluminum chloride, sodium nitrite, and sodium hydroxide. Quercetin was used as a standard reference. Chloroform extract showed highest content of flavonoids with 30.88 mg QE/g dry wt., followed by petroleum ether extract which was 22.05 mg mg QE/g dry wt., and methanol extract has the least value with 7.35 mg QE/g dry wt. The total phenolic content of the plant was estimated based on the reaction of Folin-Ciocalteu reagent using gallic acid as the standard reference. Again, the chloroform extract had the highest content with 7.54 mg GAE/g dry wt., while methanol extract contained 5.03 mg GAE/g dry wt., and petroleum ether extract contained 3.63 mg mg GAE/g dry wt. Our study shows that I. cylindrica is a good source of antioxidants.

Polyphenol Composition and Antioxidant Activity of Andrographis paniculata L. Nees

2017 ◽  
Vol 13 (4) ◽  
pp. 33-46 ◽  
Author(s):  
Praveen N
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
High Performance ◽  
Reducing Power ◽  
High Performance Liquid Chromatographic ◽  
Andrographis Paniculata ◽  
Total Phenolic ◽  
Butanol Extract ◽  
The Individual

The aim of the present study was to establish the polyphenolic profile and antioxidant activity of the leaves of Andrographis paniculata (kalmegh) that could be potentially used for the benefit of mankind. An ultra high performance liquid chromatographic method was used to identify and quantify the individual phenolic compounds of the kalmegh leaves. A total of 20 polyphenolic compounds were identified and quantified in kalmegh leaves, including hydroxybenzoic acids, hydroxycinnamic acids, flavonols and other group of phenolic compounds. The UPLC analysis of the phenolic compounds revealed that salicylic acid was the dominant phenolic compound present in the leaves extract followed by gallic acid, chlorogenic acid, veratric acid and protocatechuic acid. The kalmegh leaves were extracted with 4 different solvents (ethyl acetate, methanol, butanol and water) and screened for total phenolic content (TPC) and antioxidant activity. The antioxidant activity of kalmegh was assessed by evaluating the 1, 1-diphenyl-2-picrylhydrazyl (DPPH), reducing power and phosphomolybdenum assay. Ethyl acetate and butanol extract exhibited the highest phenolic

scholarly journals GCTOF-MS and HPLC Identification of Phenolic Compounds with Different Fractional Extracts of Lepironia articulata -

2020 ◽  
Vol 10 (1) ◽  
pp. 1-6
Author(s):  
Rashidi Othman ◽  
Razanah Ramya ◽  
Norazian Mohd. Hassan   ◽  
Suhair Kamoona
Keyword(s):  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Phenolic Content ◽  
Intrinsic Value ◽  
Total Phenolic ◽  
Hydroxybenzoic Acid ◽  
Active Elements ◽  
The Family ◽  
Microplate Reader

Cyperaceae species have an intrinsic value as a source of active elements with biological activity from the family of monocotyledonous known as sedges. Sedges grow in all types of soils associated with wetlands or poor soils. The aim of this present study is to evaluate the content of phenolic compounds by qualitative and quantitive analysis on Lepironia articulata. Dried leaves of L. articulata were successfully extracted by using water extraction then separated with different solvent polarities; petroleum ether, ethyl acetate and butanol fractions before being analysed using GCTOF-MS, microplate reader and HPLC. The result from the GCTOF-MS analysis of fractional extracts showed that 48 compounds were found in petroleum ether, ethyl acetate and butanol extracts. From those extracts, only six phenolic compounds were identified in ethyl acetate and butanol extracts which were 2-Methoxy-4-vinylphenol, Phenol, 2,4-bis(1,1-dimethylethyl)-, 4-Hydroxybenzaldehyde, Catechol, Phenol, 2-methoxy- and Vanillin. The total phenolic content was found to be 984.63 ± 5.96 ?g GAE/g DW in L. articulata. Quantitative analysis of individual phenolic acid by HPLC showed the predominant amount of Vanillic acid (0.48 ± 0.00 ?g/g DW) in ethyl acetate while 4-Hydroxybenzoic acid and Caffeic acid, both of which were 0.12 ± 0.00 ?g/g DW in butanol extracts. In the present study, the plant extracts demonstrated the highest phenolic compound detected in ethyl acetate and butanol.

scholarly journals in vitro Antioxidant Activity, Total Phenolic Content and Antimicrobial Activity of Coleus forskohlii Grown in Al-Baha, Saudi Arabia

2020 ◽  
Vol 32 (8) ◽  
pp. 2033-2037 ◽  
Author(s):  
Abdelfatah A. Fadlelmula ◽  
Abdulaziz Y. Al-Ghamdi ◽  
Mohamed O.M. Abdalla
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Antitumor Agents ◽  
Petroleum Ether Extract ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Coleus Forskohlii ◽  
Ic50 Value

In this study, the active ingredients from Coleus forskohlii were detected and evaluated their antioxidant and antimicrobial activities. Coleus forskohlii leaves were collected, air dried, powdered and extracted first with aqueous ethanol and secondly with petroleum ether, chloroform, ethyl acetate and n-butanol successively. The chemical analysis of the extracts showed the existence of (flavonoids, alkaloids, tannins, terpenoids, steroids, saponins and sugars). Furthermore, the extracts were subjected to analysis of total phenolic contents. Where the n-butanol exhibited highest TPC value (384.23±15.86 mg GAE/g), followed by ethyl acetate (199.73±29.35 mg GAE/g) then petroleum ether (96.97±61.29 mg GAE/g), ethanol (90.80±17.51 mg GAE/g), and eventually chloroform extract exhibited the lowest (TPC) value. In addition to that the antioxidant status of the different extracts of Coleus forskohlii was detected. Butanolic extract showed the highest radical scavenging (96.33±1.53 %, lowest IC50 value 0.03) followed by ethyl acetate extract (46.67±3.50 %, IC50 value 0.07). Eventually the antimicrobial activity tests were carried out for all the plant extracts. Where n-butanol showed the maximum inhibition (14±0.70 mm). All the pathogens except C. albicans exhibited moderate response towards petroleum ether extract. C. albicans was sensitive towards ethanol extract only (14.00±1.41), whereas the other Gram-positive and Gram-negative bacteria exhibited high impedance to the ethanol, chloroform, and ethyl acetate extracts. This study scientifically props the usage of whole plant as a medicine for several surface bacterial and fungal strike in folk medicine, in addition to this the plant may serve as a exporter for additional development of indigenous antioxidant and antitumor agents.

scholarly journals Qualitative and Quantitative Phenolic Compounds Analysis of Dicranopteris linearis Different Fractional Polarities Leaves Extract -

2020 ◽  
Vol 10 (1) ◽  
pp. 7-12
Author(s):  
Rashidi Othman ◽  
Razanah Ramya ◽  
Norazian Mohd. Hassan   ◽  
Suhair Kamoona
Keyword(s):  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Caffeic Acid ◽  
Petroleum Ether Extract ◽  
Total Phenolic ◽  
Traditional Values ◽  
Medicinal Value ◽  
Open Ground ◽  
Dicranopteris Linearis

Dicranopteris linearis occupies in an open ground that contains poor soils and often colonizing disturbed space that partly shaded area. It has been known for various traditional values including medicinal, edible food, soil erosion protection, pen and furniture. Even though the plants possess both economic and medicinal value, they still form the neglected group of a plant. The present study was carried out to characterize the phenolic compounds in D. linearis leaves extract in different fractional polarities qualitative and quantitatively. Dried leaves of D. linearis were successfully extracted by using water extraction before separated by petroleum ether, ethyl acetate and butanol fractions. All the fractional extracts have been analysed by using GCTOF-MS and HPLC. The result from GCTOF-MS analysis of fractional extracts showed 38 compounds found in petroleum ether, ethyl acetate and butanol extracts. However, only four phenolic compounds were identified through HPLC analysis in ethyl acetate and butanol extracts which were 2-Methoxy-4-vinylphenol, Vanillin, 4-Hydroxybenzaldehyde and 4-Hydroxybenzohydrazide. The results revealed that D. linearis contained 699.83 ± 6.26 ?g GAE /g DW of total phenolic acid whereas individual phenolic acids were predominantly caffeic acid (0.44 ± 0.01 ?g/g DW) and ferulic acid (0.22 ± 0.00 ?g/g DW) in ethyl acetate and caffeic acid (0.10 ± 0.00 ?g/g DW) and 2-Coumaric acid (0.44 ± 0.00 ?g/g DW) in butanol extracts. In the present study, the plant extracts demonstrated the highest phenolic compound detected in ethyl acetate and butanol compared to petroleum ether extract.

scholarly journals Determination of some phenolic acids in Algerian propolis

2021 ◽  
Vol 32 (2) ◽  
pp. 120-124
Author(s):  
Abdelkrim Rebiai ◽  
Bachir Ben Seghir ◽  
Hadia Hemmami ◽  
Soumeia Zeghoud ◽  
Mohamed Lakhder Belfar ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Phenolic Acids ◽  
High Performance ◽  
Quantitative Method ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Coumaric Acid ◽  
Plant Exudates ◽  
North And South

Abstract Propolis is a resinous material collected by bees from various plant exudates, rich in well-known phenolic compounds, such as phenolic acids, that are important to health. Extracts of propolis are very complex matrices that are hard to test. The purpose of this study was to characterize some of the propolis phenolics that were collected from five different districts in Algeria. The High-Performance Liquid Chromatography (HPLC), a modern quantitative method, has been adopted to identify the phenolic acids. Moreover, total phenolic content of four different phenolic acids were identified, with the most abundant being chlorogenic acid, followed by caffeic acid, gallic acid, and p-coumaric acid, the obtained ratios from phenolic acids being in the range of 52.193 to 148.151 μg/g, 0.043 to 7.128 mg/g, 0.328 to 0.440 mg/g and 0.328 to 0.440 mg/g, respectively. Overall, our analysis indicates that all the samples of propolis tested are healthy sources of phenolic acids and the significant differences in the concentrations of the acids were observed for propolis samples from north and south of Algeria. It is probably the effect of different conditions of the collection of the resin and secrets by bees.

scholarly journals Comparative Analysis of in Vitro Antioxidant and Cytotoxic Activity of Unripe and Ripe Fruits of Solanum sisymbriifolium

2020 ◽  
Vol 8 (6) ◽  
Author(s):  
Farhana Momen ◽  
Rashu Barua ◽  
Md. Golam Kabir
Keyword(s):  
Petroleum Ether ◽  
Phenolic Content ◽  
Petroleum Ether Extract ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Ether Extract ◽  
Unripe Fruit ◽  
Lc50 Value ◽  
Solanum Sisymbriifolium ◽  
Unripe Fruits

Solanum sisymbriifolium Lam. (Solanaceae), commonly referred to as Kantikari (Bengali), sticky nightshade, or litchi tomato. Despite, the traditional use of this plant for several ailments, very little is known about the phytochemical and pharmacological content of the edible ripe berries of this medicinal plant. Ethanol and petroleum ether extract of the ripe and unripe fruits of Solanum sisymbriifolium were examined by using standard analytical methods to determine and compare their bioactive chemical constituents, total phenolic content, antioxidant, and cytotoxic potential. Quantitative phytochemical screening showed that the presence of medicinally active secondary metabolites like alkaloids, flavonoids, and saponins were detected in both ripe and unripe fruits. Antioxidant activity and total phenolic content were found to be more pronounced in ripe fruit extract, whereas unripe fruit extracts showed moderate cytotoxic activity. DPPH free radical scavenging method demonstrated that IC50 value of ethanol and petroleum ether extract of ripe fruits were 194.40μg/ml and 436μg/ml, and for unripe fruit the values were 689.82μg/ml and 1411.23μg/ml. In brine shrimp lethality bioassay, the LC50 value of unripe fruits was 230.6559μg/ml for ethanol extract and 314.9227μg/ml for petroleum ether extract. The LC50 value of the ethanol extract of ripe fruits was 456.1021μg/ml and for petroleum ether extract was 1,017.6848μg/ml. The results of this study showed that the litchi tomato not only has the potential to be a rich source of an edible compound due to the presence of profound phytochemical constituents but also can be used as a source of pharmacological references.

scholarly journals QUANTITATIVE PHYTOCHEMICAL SCREENING, THIN-LAYER CHROMATOGRAPHY ANALYSIS, HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY FINGERPRINTING, AND ANTIOXIDANT ACTIVITY OF LEAVES OF DIOSPYROS MONTANA (ROXB.)

2019 ◽  
pp. 325-331
Author(s):  
ABHIJEET V PURI
Keyword(s):  
Antioxidant Activity ◽  
Thin Layer ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Butylated Hydroxytoluene ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Layer Chromatography

Objective: The objective of this study was to investigate important phytochemical constituents and antioxidant potential of Diospyros montana Roxb. leaves belonging to the family Ebenaceae. Methods: Leaves were exhaustively extracted with ethanol and fractionated into petroleum ether, chloroform, and ethyl acetate extracts. The various fractions were further analyzed for phytochemical composition and concentration-dependent antioxidant activity using conventional methods and high-performance thin-layer chromatography (HPTLC) fingerprinting. Since leaves contained phenolic compounds, extracts were evaluated for total phenolic content, flavonoids contents, and in-vitro antioxidant activity. Antioxidant potential was assessed using parameters such as superoxide radical scavenging, nitric oxide inhibition, and β-carotene/linoleic acid antioxidant activity. Results: Primitive phytochemical investigation highlighted the presence of steroids, saponins, flavonoids, alkaloids, and tannins which were confirmed by TLC and HPTLC fingerprinting. The antioxidant activity of leaf extracts decreased in the following order ethyl acetate > ethanolic > chloroform > petroleum ether and it was comparable with standards such as ascorbic acid and butylated hydroxytoluene. Conclusion: The present study concludes that the ethanolic extract and fractions of D. montana (Roxb.) leaves have prominent antioxidant activity comparable to standards. Therefore, D. montana (Roxb.) leaves may be used as a probable source of natural antioxidants in the pharmaceutical industry.

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