scholarly journals Differences in in vitro hydrolysis and fermentation among and within high-fiber ingredients using a modified three-step procedure in growing pigs1,2

2017 ◽  
Vol 95 (12) ◽  
pp. 5497-5506 ◽  
Author(s):  
Z. Huang ◽  
P. E. Urriola ◽  
I. J. Salfer ◽  
M. D. Stern ◽  
G. C. Shurson
Keyword(s):  
High Fiber ◽  
In Vitro Hydrolysis ◽  
Step Procedure

scholarly journals Stimulation of Interleukin-10 Production by Acidic β-Lactoglobulin-Derived Peptides Hydrolyzed with Lactobacillus paracasei NCC2461 Peptidases

2004 ◽  
Vol 11 (2) ◽  
pp. 266-271 ◽  
Author(s):  
Guénolée Prioult ◽  
Sophie Pecquet ◽  
Ismail Fliss
Keyword(s):  
Oral Tolerance ◽  
Immunosuppressive Agents ◽  
Interleukin 10 ◽  
Lactobacillus Paracasei ◽  
In Vitro Hydrolysis ◽  
Ifn Γ ◽  
Immunomodulatory Peptides ◽  
Β Lactoglobulin

ABSTRACT We have previously demonstrated that Lactobacillus paracasei NCC2461 may help to prevent cow's milk allergy in mice by inducing oral tolerance to β-lactoglobulin (BLG). To investigate the mechanisms involved in this beneficial effect, we examined the possibility that L. paracasei induces tolerance by hydrolyzing BLG-derived peptides and liberating peptides that stimulate interleukin-10 (IL-10) production. L. paracasei peptidases have been shown to hydrolyze tryptic-chymotryptic peptides from BLG, releasing numerous small peptides with immunomodulating properties. We have now shown that acidic tryptic-chymotryptic peptides stimulate splenocyte proliferation and gamma interferon (IFN-γ) production in vitro. Hydrolysis of these peptides with L. paracasei peptidases repressed the lymphocyte stimulation, up-regulated IL-10 production, and down-regulated IFN-γ and IL-4 secretion. L. paracasei NCC2461 may therefore induce oral tolerance to BLG in vivo by degrading acidic peptides and releasing immunomodulatory peptides stimulating regulatory T cells, which function as major immunosuppressive agents by secreting IL-10.

PSXIII-12 Exploring nutritional differences of canola seeds and bio-processing co-products (meals, pellets) from different processing plants/companies in Canada and China for dairy cattle

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 461-462
Author(s):  
A Oliveira ◽  
P Yu
Keyword(s):  
Dairy Cows ◽  
Canola Meal ◽  
Human Consumption ◽  
Livestock Feed ◽  
Step Procedure ◽  
Processing Plants ◽  
Low Erucic Acid ◽  
Intestinal Digestibility

Abstract Canola was created as a low erucic acid and low glucosinolate seed, to produce high quality oil for human consumption and meal for use in livestock feed. China is an important user of Canadian canola products (seeds, oil, and meal). The extraction of the oil from the seed produces a co-product called canola meal. This meal is rich in protein and is used as a protein source in animal diets. However, differences in the characteristics of the seeds, or processing methods during oil extraction may affect the quality of this co-product. Plus, the synthesis of tissues and milk is related to the amino acids available to the animal for absorption in the small intestine. This study aimed to determine if there are significant differences in the intestinal digestibility (in vitro) of CP and DM between canola seeds and meals from different companies in Canada and to determine if there are significant differences between them in Canada and China. The three-step procedure was applied on residues from a 12-hour rumen incubation in fistulated dairy cows to estimate the intestinal digestibility of CP and DM. There were significant differences (P < 0.05) for TDDM (Total digestible dry matter) and IDP (intestinal digestibility of protein) of the meals between countries. The samples from China had higher TDDM (83.76% versus 81.53%, P = 0.018), while Canada’s had higher IDP (68.51% versus 65.28%, P = 0.016). No significant differences were observed within countries. Based on the material analyzed during this study, it is safe to affirm that there are no significant differences in the digestibility of DM and CP between Canada and China. It was concluded that the quality of the canola seeds or meals produced in both Canada and China were similar when used in dairy rations. Key words: canola seeds and bio-processing co-products (meals, pellets), nutritional differences, dairy cows

249 Increasing Structural Fiber Improves Growth Performance of Nursery Pigs

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 93-93
Author(s):  
Sara Ebarb ◽  
Sabrina May ◽  
Mark D Newcomb
Keyword(s):  
Growth Performance ◽  
Mixed Model ◽  
Linear Mixed Model ◽  
Two Phase ◽  
Nursery Pigs ◽  
High Fiber ◽  
Rice Hulls ◽  
Fiber Treatment ◽  
Wheat Middlings

Abstract Fiber ingredients in swine diets have various components that affect the intestinal tract distinctively. The objective of this study was to determine the effect of structural fiber sources on growth performance of nursery pigs. A total of 1,760 weanling pigs (initial BW = 6.12 ± 0.3 kg, 16 reps/trt, 22 pigs/pen) were used in a study with 5 dietary treatments: 1) Complex nursery diet with no additional fiber (CON); 2) CON + 2% rice hulls; 3) CON + 4% rice hulls; 4) CON + 6% wheat middlings; 5) CON + 12% wheat middlings. Fiber ingredients were added at the expense of corn and some processed soybean meal to maintain similar dietary protein levels. Using an in vitro fermentation estimation of fermentation for rice hulls and wheat middlings, diets were constructed to add similar levels of structural (non-fermentable NDF) fiber between low and high additions of rice hulls and wheat middlings. Pens across two barns were randomly allotted independent of one another and within a location block to one of the five treatments on d 0 of a two-phase study (d 0–11 and d 11–20.5 post-weaning). Data were analyzed by general linear model in R. Mortality and removal data were analyzed as a generalized linear mixed model with a binomial distribution. Contrasts tested the effect of additional fiber (CON vs treatments 2–5), effect of medium vs high fiber (treatment 2/4 vs 3/5), effect of source of fiber (treatment 2/3 vs 4/5), and the interaction of level and source of fiber. Overall (Table 1), additional fiber resulted in increased ADFI (P < 0.05) and tended to increase ADG (P < 0.10). Probability of mortality and removal was reduced (P < 0.05) when additional fiber was included. In summary, increasing the level of structural fiber improved performance and livability of nursery pigs.

Preimplantation development of gynogenetic diploid mouse embryos

Development ◽  
1982 ◽  
Vol 69 (1) ◽  
pp. 215-222
Author(s):  
Ewa Borsuk
Keyword(s):  
Cytochalasin B ◽  
Polar Body ◽  
Mouse Embryos ◽  
Fertilization In Vitro ◽  
Body Formation ◽  
Male Pronucleus ◽  
Step Procedure ◽  
Gynogenetic Diploid ◽  
Polar Body Formation

Diploid gynogenetic mouse embryos were produced in a three-step procedure: fertilization in vitro, suppression of the 2nd polar body formation by Cytochalasin B, and microsurgical removal of the male pronucleus. The operated eggs were transplanted to the oviduct of recipient females for 72 or 96 h. The overall recovery rate was 73%, but compacted morulae and blastocysts constituted only 28·6% of transplanted eggs. After 72 h blastocysts were rare (3·5%) but 24 h later their incidence increased to 21·2%. In eggs homozygous for T6 chromosome it was possible to prove karyologically that the male pronucleus was effectively removed and that the diploid genome was of purely maternal origin.

Effect of in vitro hydrolysis on coconut milk digestibility

2019 ◽  
Vol 59 (5) ◽  
pp. 380-384
Author(s):  
Y. Benaissa ◽  
S. Addou ◽  
W. Dib ◽  
S. Benhatchi ◽  
A. Mehidi ◽  
...  
Keyword(s):  
Coconut Milk ◽  
In Vitro Hydrolysis
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