Standardized ileal digestible amino acids and digestible energy contents in high-protein distiller's dried grains with solubles fed to growing pigs1

2017 ◽  
Vol 95 (8) ◽  
pp. 3591-3597 ◽  
Author(s):  
Y. Rho ◽  
C. Zhu ◽  
E. Kiarie ◽  
C. F. M de Lange
Keyword(s):  
Amino Acids ◽  
High Protein ◽  
Digestible Energy ◽  
Digestible Amino Acids

Dietary protein paradox: decrease of amino acid availability induced by high-protein diets

1993 ◽  
Vol 264 (6) ◽  
pp. G1057-G1065 ◽  
Author(s):  
C. Moundras ◽  
C. Remesy ◽  
C. Demigne
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Dietary Protein ◽  
High Protein ◽  
Glutamine Metabolism ◽  
Metabolic Adaptation ◽  
Casein Diet ◽  
Serine Dehydratase ◽  
High Protein Diets ◽  
Protein Diets

The aim of the present study was to evaluate the effect of changes in dietary protein level on overall availability of amino acids for tissues. For this purpose, rats were adapted to diets containing various concentrations of casein (7.5, 15, 30, and 60%) and were sampled either during the postprandial or postabsorptive period. In rats fed the protein-deficient diet, glucogenic amino acids (except threonine) tended to accumulate in plasma, liver, and muscles. In rats fed high-protein diets, the hepatic balance of glucogenic amino acids was markedly enhanced and their liver concentrations were consistently depressed. This response was the result of a marked induction of amino acid catabolism (a 45-fold increase of liver threonine-serine dehydratase activity was observed with the 60% casein diet). The muscle concentrations of threonine, serine, and glycine underwent changes parallel to plasma and liver concentrations, and a significant reduction of glutamine was observed. During the postabsorptive period, adaptation to high-protein diets resulted in a sustained catabolism of most glucogenic amino acids, which accentuated the drop in their concentrations (especially threonine) in all the compartments studied. The time course of metabolic adaptation from a 60 to a 15% casein diet has also been investigated. Adaptation of alanine and glutamine metabolism was rapid, whereas that of threonine, serine, and glycine was delayed and required 7-11 days. This was paralleled by a relatively slow decay of liver threonine-serine dehydratase (T-SDH) activity in contrast to the rapid adaptation of pyruvate kinase activity after refeeding a high-carbohydrate diet.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Apparent ileal digestibility of amino acids and metabolisable energy value in grains for broilers

2008 ◽  
Vol 14 (4) ◽  
pp. 325 ◽  
Author(s):  
S. PERTTILÄ ◽  
J. VALAJA ◽  
T. JALAVA
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Broiler Chickens ◽  
Crude Protein ◽  
Dry Matter ◽  
Nitrogen Excretion ◽  
Ileal Digestibility ◽  
Metabolisable Energy ◽  
Feed Protein ◽  
Digestible Amino Acids

Using ileal digestible amino acids in feed optimising will intensify feed protein utilizing and decrease nitrogen excretion to the environment. The study determined the apparent ileal digestibility (AID) coefficients of amino acids in barley, wheat, oats, triticale, maize, and dehulled oats in the diets of 180 Ross broiler chickens (aged 24–35 days). The birds were fed semi-purified diets that contained grain as the sole protein source and chromium-mordanted straw as an indigestible marker. The AID coefficients of the nutrients were assessed using the slaughter technique, and the apparent metabolisable energy (AME) was determined using total excreta collection. The ileal digestibility of the dry matter and organic matter were the highest in maize. The AME of maize was higher than that of other cereals. The ileal digestibility of crude protein was higher in wheat than that in barley, oats and dehulled oats. The AME of wheat was similar to that of barley and oats but lower than that of triticale and dehulled oats. The amino acid AID was highest in wheat (0.86) and triticale (0.85) and lowest in oats (0.79) and barley 0.77). The average amino acid AID was 0.81 in dehulled oats. The threonine AID was the same in all tested ingredients. The lysine, methionine, and cystine AID coefficients were 0.81, 0.79, and 0.71 respectively for barley; 0.86, 0.84, and 0.38 respectively for oats; 0.87, 0.86, and 0.53 respectively for dehulled oats; 0.84, 0.90, and 0.66 respectively for maize; 0.89, 0.88, and 0.77 respectively for triticale; and 0.87, 0.85, and 0.71 respectively for wheat. Results indicated that AME –values of domestic grains (barley, oats and wheat) are in the same level. Especially, low AME value of wheat needs further investigation.;

scholarly journals Isolation and Characterization of Red Bean and Green Bean Protein using the Extraction Method and Isoelectric pH

2020 ◽  
Vol 2 (2) ◽  
pp. 77-85
Author(s):  
Slamet Hadi Kusumah ◽  
Robi Andoyo ◽  
Tita Rialita
Keyword(s):  
Amino Acids ◽  
Protein Content ◽  
Electrophoretic Mobility ◽  
Essential Amino Acids ◽  
High Protein ◽  
Green Bean ◽  
Isolation And Characterization ◽  
Isoelectric Ph ◽  
Red Bean ◽  
Bean Protein

Stunting children need food intake with higher protein and essential amino acids such as lysine and leucine than normal children. Red Beans (Phaseolus Vulgaris L.) and Green Beans (Phaseolus Radiatus L.) have protein content, respectively 22.07 ± 0.13% and 19.99 ± 0.07%, and are rich in essential amino acids lysine and leucine needed by a stunting child. The Development of food products with high protein concentrations (high protein food) such as isolates/protein concentrates is an important subject that must be done. This study aims to determine the technique of isolating red bean and green bean protein through extraction and deposition methods at isoelectric pH. The research method used is an experimental method with descriptive data analysis. Identification of isoelectric pH of red bean and green bean protein is done through electrophoretic mobility (cm2/Vs) testing on samples that have been conditioned at 3 different depositional pHs namely pH 3.00, 4.00, and 5.00. The results showed the isoelectric pH value of red bean and green bean protein, namely pH 4.56 and pH 4.81 respectively, where the pH had electrophoretic mobility values equal to zero. The process of isolating red bean and green bean protein produces protein powder with a protein content of 79.22 ± 0.06% and 80.69 ± 0.06%, respectively. Based on the weight yield, red bean and green bean protein concentrate powder had a yield of 14.88% and 16.75%, respectively.

scholarly journals Response of Broiler Chicks to Low-Protein-L-Valine Supplemented Diets Formulated Based on Digestible Amino Acids

2017 ◽  
Vol 8 (2) ◽  
pp. 13-19
Author(s):  
Amira Refaie ◽  
A. Abdallah ◽  
Abeer Khosht ◽  
Hemat Abdel Magied ◽  
Heba Habib ◽  
...  
Keyword(s):  
Amino Acids ◽  
Broiler Chicks ◽  
Protein L ◽  
Low Protein ◽  
Digestible Amino Acids

The effect of wheat cultivar, growing environment, and enzyme supplementation on digestibility of amino acids by broilers

1998 ◽  
Vol 78 (3) ◽  
pp. 335-342 ◽  
Author(s):  
M. R. Bedford ◽  
T. A. Scott ◽  
F. G. Silversides ◽  
H. L. Classen ◽  
M. L. Swift ◽  
...  
Keyword(s):  
Amino Acids ◽  
Broiler Chicken ◽  
Wheat Cultivar ◽  
High Quality Protein ◽  
Xylanase Enzyme ◽  
Growing Environment ◽  
Digestible Protein ◽  
Enzyme Supplementation ◽  
Digestible Amino Acids ◽  
Broiler Feed

Fifty-four samples of wheat, representing duplicate samples of nine varieties grown in three environments, were included in chick bioassays using rapidly growing broilers, complete diets, and enzyme supplementation to remove the deleterious effects of non-starch polysaccharides. An insoluble ash marker was included in the diets, which allowed calculation of apparent digestibilities of CP and AA in the grain from analysis of the ileal contents obtained after sacrifice of the chicks at 17 d of age. The wheat cultivars contained from 13.5 to 16.8% CP and differed in the percentage of specific AA, with Pro, Glu and Phe making up proportionately larger parts of high CP samples than low CP samples. Without enzyme supplementation, the digestibility of CP varied from 83 to 88% and that of specific AA from 76 to 94%, determined in part by the class and variety of wheat. Xylanase enzyme supplementation improved the digestibility of protein by an average of 4%, and reduced the differences in digestibility between wheat samples. These results suggest that variation between wheat samples should be taken into consideration when formulating broiler feed, especially when formulation is for specific AA. Enzyme supplementation may provide a partial alternative to using high-quality protein sources or supplementing with synthetic AA. Key words: Broiler chicken, digestible amino acids, digestible protein, wheat, enzyme

High Protein Intake during Continuous Hemodiafiltration: Impact on Amino Acids and Nitrogen Balance

2002 ◽  
Vol 25 (4) ◽  
pp. 261-268 ◽  
Author(s):  
R. Bellomo ◽  
H. K. Tan ◽  
S. Bhonagiri ◽  
I. Gopal ◽  
J. Seacombe ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Nitrogen Balance ◽  
Protein Intake ◽  
Protein Supplementation ◽  
High Protein ◽  
Total Serum ◽  
Amino Acid Supplementation ◽  
Continuous Hemodiafiltration ◽  
High Protein Intake

Aims To study the effect of combined continuous veno-venous hemodiafiltration (CVVHDF) and high (2.5 g/kg/day) parenteral amino acid supplementation on nitrogen balance, amino acid losses and azotemic control in a cohort of patients with severe acute renal failure (ARF). Methods We administered 2.5 grams/kg/day of amino acids intravenously to seven critically ill patients with ARF. We obtained paired blood and ultrafiltrate (UF) samples (n=20) and calculated amino acid clearances and losses, nitrogen balance, protein catabolic rate and total nitrogen losses. Results The median total serum amino acid concentration was high at 5.2 mmol/L with particularly high concentrations of ornithine, lysine, and phenylalanine, but a low level of histidine. The median overall amino acid clearance was 18.6 ml/min (range: 12 to 29 ml/min). UF losses as percentage of administered dose were high for tyrosine (53.6 %) but low for methionine (3.0 %) and arginine (2.3 %). A positive nitrogen balance was achieved in 7 (35%) of the 20 study days with an overall median nitrogen balance of -1.8 g/day. Urea levels were maintained at a median of 26.6 mmol/L. Conclusions High protein intake increases the serum concentrations of most amino acids. Such protein supplementation, when coupled with CVVHDF, achieves a slightly negative overall nitrogen balance in extremely catabolic patients while still allowing adequate azotemic control.

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