Modeling of intramuscular lipids in different muscles in bulls, steers, and cows1

2015 ◽  
Vol 93 (10) ◽  
pp. 5073-5082 ◽  
Author(s):  
M. Blanco ◽  
J. Agabriel ◽  
B. Picard ◽  
D. Micol ◽  
C. Jurie ◽  
...  
Keyword(s):  
Intramuscular Lipids

Restoring AS160 phosphorylation rescues skeletal muscle insulin resistance and fatty acid oxidation while not reducing intramuscular lipids

2009 ◽  
Vol 297 (5) ◽  
pp. E1056-E1066 ◽  
Author(s):  
Hakam Alkhateeb ◽  
Adrian Chabowski ◽  
Jan F. C. Glatz ◽  
Brendon Gurd ◽  
Joost J. F. P. Luiken ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Skeletal Muscle ◽  
Glucose Transport ◽  
Acid Oxidation ◽  
Glut4 Translocation ◽  
Muscle Insulin Resistance ◽  
Palmitate Oxidation ◽  
Skeletal Muscle Insulin Resistance ◽  
Ampk Phosphorylation ◽  
Intramuscular Lipids

We examined whether AICAR or leptin rapidly rescued skeletal muscle insulin resistance via increased palmitate oxidation, reductions in intramuscular lipids, and/or restoration of insulin-stimulated AS60 phosphorylation. Incubation with palmitate (2 mM, 0–18 h) induced insulin resistance in soleus muscle. From 12–18 h, palmitate was removed or AICAR or leptin was provided while 2 mM palmitate was maintained. Palmitate oxidation, intramuscular triacylglycerol, diacylglycerol, ceramide, AMPK phosphorylation, basal and insulin-stimulated glucose transport, plasmalemmal GLUT4, and Akt and AS160 phosphorylation were examined at 0, 6, 12, and 18 h. Palmitate treatment (12 h) increased intramuscular lipids (triacylglycerol +54%, diacylglycerol +11%, total ceramide +18%, C16:0 ceramide +60%) and AMPK phosphorylation (+118%), whereas it reduced fatty acid oxidation (−60%) and insulin-stimulated glucose transport (−70%), GLUT4 translocation (−50%), and AS160 phosphorylation (−40%). Palmitate removal did not rescue insulin resistance or associated parameters. The AICAR and leptin treatments did not consistently reduce intramuscular lipids, but they did rescue palmitate oxidation and insulin-stimulated glucose transport, GLUT4 translocation, and AS160 phosphorylation. Increased AMPK phosphorylation was associated with these improvements only when AICAR and leptin were present. Hence, across all experiments, AMPK phosphorylation did not correlate with any parameters. In contrast, palmitate oxidation and insulin-stimulated AS160 phosphorylation were highly correlated ( r = 0.83). We speculate that AICAR and leptin activate both of these processes concomitantly, involving activation of unknown kinases in addition to AMPK. In conclusion, despite the maintenance of high concentrations of palmitate (2 mM), as well as increased concentrations of intramuscular lipids (triacylglycerol, diacylglycerol, and ceramide), the rapid AICAR- and leptin-mediated rescue of palmitate-induced insulin resistance is attributable to the restoration of insulin-stimulated AS160 phosphorylation and GLUT4 translocation.

Study of the branched hydrocarbon fraction of intramuscular lipids from Iberian dry-cured ham

Meat Science ◽  
2005 ◽  
Vol 69 (1) ◽  
pp. 129-134 ◽  
Author(s):  
M.J. Petrón ◽  
J.F. Tejeda ◽  
E. Muriel ◽  
J. Ventanas ◽  
T. Antequera
Keyword(s):  
Hydrocarbon Fraction ◽  
Dry Cured Ham ◽  
Intramuscular Lipids

Lipid traits of muscles as related to genotype and fattening diet in Iberian pigs: total intramuscular lipids and triacylglycerols

Meat Science ◽  
2002 ◽  
Vol 60 (4) ◽  
pp. 357-363 ◽  
Author(s):  
J.F. Tejeda ◽  
G. Gandemer ◽  
T. Antequera ◽  
M. Viau ◽  
C. Garcı́a
Keyword(s):  
Intramuscular Lipids

scholarly journals Effects of high linolenic acid diet supplemented with synthetic or natural antioxidant mix on live performance, carcass traits, meat quality and fatty acid composition of Longissimus thoracis et lumborum muscle of medium-heavy pigs

2021 ◽  
Vol 33 (2) ◽  
pp. 117-128
Author(s):  
Anna Maria Belmonte ◽  
Paolo Macchioni ◽  
Giovanna Minelli ◽  
Corina Scutaru ◽  
Luisa Antonella Volpelli ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Linolenic Acid ◽  
Meat Quality ◽  
Acid Composition ◽  
Carcass Traits ◽  
Natural Antioxidants ◽  
Grape Skin ◽  
Acid Diet ◽  
Intramuscular Lipids

We studied the effect of a high linolenic acid diet supplementation with synthetic (vitamin E + selenium) or veg-etal mix rich in natural antioxidants (grape skin + oregano) on live performances, carcass and meat quality, fatty acid composition and oxidative stability of intramuscular lipids of Longissimus thoracis et lumborum muscle in medium-heavy pigs. Neither carcass traits nor chemical proximate composition of meat was affected by dietary treatments. Linseed dietary inclusion reduced the n-6:n-3 polyunsaturated fatty acids ratio and increased long-chain n-3 precursor, fundamental for human health. Our results offer new opportunities to use products more acceptable by consumers and are more eco-friendly.

scholarly journals Single step extraction and derivatization of intramuscular lipids for fatty acid Ultra Fast GC analysis: application on pig thigh

2017 ◽  
Vol 54 (3) ◽  
pp. 601-610 ◽  
Author(s):  
Eleonora Laura De Paola ◽  
Giuseppe Montevecchi ◽  
Francesca Masino ◽  
Andrea Antonelli ◽  
Domenico Pietro Lo Fiego
Keyword(s):  
Fatty Acid ◽  
Single Step ◽  
Gc Analysis ◽  
Fast Gc ◽  
Analysis Application ◽  
Intramuscular Lipids

Muscle contraction increases palmitate esterification and oxidation and triacylglycerol oxidation

1998 ◽  
Vol 275 (5) ◽  
pp. E888-E896 ◽  
Author(s):  
D. J. Dyck ◽  
A. Bonen
Keyword(s):  
Lipid Oxidation ◽  
Total Energy ◽  
Muscle Contraction ◽  
Absolute Increase ◽  
Palmitate Oxidation ◽  
Oxidation Rates ◽  
Intramuscular Lipid ◽  
Muscle Energy ◽  
Intramuscular Lipids ◽  
Total Muscle

We examined the oxidation and esterification of palmitate and the hydrolysis and oxidation of intramuscular lipids in isolated soleus muscles at rest and during tetanic contractions (2–40 tetani/min). Muscles were pulsed with [14C]palmitate to prelabel all intramuscular lipid pools. Muscles remained at rest or were then stimulated to contract at 2, 8, 20, or 40 tetani/min (30 min) in the presence of [3H]palmitate. Palmitate oxidation was increased 412% at 2 tetani/min ( P < 0.05) and 880% at 8 tetani/min ( P < 0.05). During contraction there was an absolute increase in esterification of palmitate to triacylglycerol in proportion with the increasing rate of palmitate oxidation. Intramuscular lipid oxidation provided ∼77% of the total muscle energy compared with ∼3% provided by exogenous palmitate under all conditions, with carbohydrate sources (glycogen and glucose) providing ∼20% of the total energy. Thus, during muscle contraction, the oxidation rates of both exogenous and intramuscular lipids are increased in proportion to each other, while concomitantly palmitate is esterified in proportion to its oxidation.

Effect of trans-fatty acid intake on insulin sensitivity and intramuscular lipids—a randomized trial in overweight postmenopausal women

Metabolism ◽  
2011 ◽  
Vol 60 (7) ◽  
pp. 906-913 ◽  
Author(s):  
Nathalie T. Bendsen ◽  
Steen B. Haugaard ◽  
Thomas M. Larsen ◽  
Elizaveta Chabanova ◽  
Steen Stender ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Insulin Sensitivity ◽  
Randomized Trial ◽  
Postmenopausal Women ◽  
Trans Fatty Acid ◽  
Fatty Acid Intake ◽  
Acid Intake ◽  
Trans Fatty Acid Intake ◽  
Intramuscular Lipids
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