Transcriptome analysis of subcutaneous adipose tissues in beef cattle using 3′ digital gene expression-tag profiling1

2012 ◽  
Vol 90 (1) ◽  
pp. 171-183 ◽  
Author(s):  
W. Jin ◽  
E. N. Olson ◽  
S. S. Moore ◽  
J. A. Basarab ◽  
U. Basu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Beef Cattle ◽  
Transcriptome Analysis ◽  
Digital Gene Expression ◽  
Adipose Tissues ◽  
Subcutaneous Adipose

scholarly journals Comparison of Metabolic Network between Muscle and Intramuscular Adipose Tissues in Hanwoo Beef Cattle Using a Systems Biology Approach

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Hyun-Jeong Lee ◽  
Hye-Sun Park ◽  
Woonsu Kim ◽  
Duhak Yoon ◽  
Seongwon Seo
Keyword(s):  
Systems Biology ◽  
Beef Cattle ◽  
Metabolic Regulation ◽  
Bos Taurus ◽  
Adipose Tissues ◽  
Chemokine Signaling ◽  
Gene Network Analysis ◽  
Chemokine Signaling Pathway ◽  
Subcutaneous Adipose

The interrelationship between muscle and adipose tissues plays a major role in determining the quality of carcass traits. The objective of this study was to compare metabolic differences between muscle and intramuscular adipose (IMA) tissues in thelongissimus dorsi(LD) of Hanwoo (Bos taurus coreanae) using the RNA-seq technology and a systems biology approach. The LD sections between the 6th and 7th ribs were removed from nine (each of three cows, steers, and bulls) Hanwoo beef cattle (carcass weight of430.2±40.66 kg) immediately after slaughter. The total mRNA from muscle, IMA, and subcutaneous adipose and omental adipose tissues were isolated and sequenced. The reads that passed quality control were mapped onto the bovine reference genome (build bosTau6), and differentially expressed genes across tissues were identified. The KEGG pathway enrichment tests revealed the opposite direction of metabolic regulation between muscle and IMA. Metabolic gene network analysis clearly indicated that oxidative metabolism was upregulated in muscle and downregulated in IMA. Interestingly, pathways for regulating cell adhesion, structure, and integrity and chemokine signaling pathway were upregulated in IMA and downregulated in muscle. It is thus inferred that IMA may play an important role in the regulation of development and structure of the LD tissues and muscle/adipose communication.

scholarly journals Transcriptome Analysis of Marchantin Biosynthesis from the Liverwort Marchantia polymorpha

2017 ◽  
Vol 12 (8) ◽  
pp. 1934578X1701200
Author(s):  
Hironobu Takahashi ◽  
Yoshinori Asakawa
Keyword(s):  
Gene Expression ◽  
Transcriptome Analysis ◽  
Transcriptome Sequencing ◽  
Biological Activities ◽  
Digital Gene Expression ◽  
Marchantia Polymorpha ◽  
Rna Seq ◽  
Gene Expression Analyses

Marchantin A, the first characterized macrocyclic bis(bibenzyls) found in the liverwort Marchantia polymorpha shows interesting biological activities such as antifungal, antimicrobial, cytotoxic, antioxidant and muscle relaxing activity. Previously, Zenk et al. reported the phenylpropane/polymalonate pathway in the biosynthesis of the marchantins in M. polymorpha. To clear this pathway, transcriptome sequencing and digital gene expression analyses of M. polymorpha were carried out by using Illumina RNA-seq system.

scholarly journals Dietary glycemic index and dietary glycemic load is associated with apelin gene expression in visceral and subcutaneous adipose tissues of adults

2019 ◽  
Vol 16 (1) ◽  
Author(s):  
Emad Yuzbashian ◽  
Golaleh Asghari ◽  
Maryam Aghayan ◽  
Mehdi Hedayati ◽  
Maryam Zarkesh ◽  
...  
Keyword(s):  
Gene Expression ◽  
Insulin Resistance ◽  
Glycemic Index ◽  
Glycemic Load ◽  
Carbohydrate Intake ◽  
Model Assessment ◽  
Total Carbohydrate ◽  
Adipose Tissues ◽  
Visceral Adipose ◽  
Subcutaneous Adipose

Abstract Background Apelin, as an adipokine, plays an important role in the pathogenesis of insulin resistance and type 2 diabetes. This study aimed to determine whether the quality and quantity of dietary carbohydrates were associated with apelin gene expression in subcutaneous and visceral adipose tissues. Methods In this cross-sectional study, 102 adults who underwent minor abdominal surgery were selected. Approximately 100 mg of subcutaneous and visceral adipose tissues were collected during the surgery to measure apelin gene expression. Anthropometric measurment, blood samples, and dietary intakes were collected before surgery. The dietary carbohydrate intake, glycemic index (GI), and glycemic load (GL) were determined. Results The average apelin concentration was 269.6 ± 98.5(pg/mL), and 16.3% of participants were insulin resistant. There was a correlation between insulin (p-value = 0.043), Homeostatic Model Assessment for Insulin Resistance (HOMA-IR)(p-value = 0.045) and apelin gene expression in visceral adipose tissue. There was a positive association of apelin gene expression with dietary GI and GL after adjustment for age, sex, and waist circumference in visceral and subcutaneous adipose tissues(p < 0.05). Apelin gene expression in visceral(p = 0.002) and subcutaneous(p = 0.003) adipose tissues was directly associated with foods with a higher GI. There was no association between total carbohydrate intake and apelin gene expression in both visceral and subcutaneous adipose tissues. Conclusions Dietary GI and GL, not total carbohydrate intake, were positively associated with apelin gene expression in both visceral and subcutaneous adipose tissues. Future studies are warranted to illustrate the chronic and acute effect of carbohydrate quality on apelin homeostasis.

Association of dietary intake of fruit and green vegetables with PTEN and P53 mRNA gene expression in visceral and subcutaneous adipose tissues of obese and non-obese adults

Gene ◽  
2020 ◽  
Vol 733 ◽  
pp. 144353
Author(s):  
Golnoosh Kadkhoda ◽  
Maryam Zarkesh ◽  
Atoosa Saidpour ◽  
Masoumeh Hajizadeh Oghaz ◽  
Mehdi Hedayati ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dietary Intake ◽  
Obese Adults ◽  
Adipose Tissues ◽  
Mrna Gene ◽  
Subcutaneous Adipose ◽  
Mrna Gene Expression ◽  
P53 Mrna

scholarly journals The relation of omentin gene expression and glucose homeostasis of visceral and subcutaneous adipose tissues in non-diabetic adults

2021 ◽  
Author(s):  
Afsoon Daneshafrooz ◽  
Emad Yuzbashian ◽  
Maryam Zarkesh ◽  
Golaleh Asghari ◽  
Parvin Mirmiran ◽  
...  
Keyword(s):  
Gene Expression ◽  
Glucose Homeostasis ◽  
Adipose Tissues ◽  
Subcutaneous Adipose

scholarly journals PSXI-27 Association of an increased marbling score by dietary glycerol supplementation with lipid metabolism gene expression in Korean cattle steers

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 383-384
Author(s):  
Seon Pil Yoo ◽  
Dilla Fassah ◽  
Myunggi Baik ◽  
sang Weon Na ◽  
Inhyuk Jeong ◽  
...  
Keyword(s):  
Gene Expression ◽  
Body Weight ◽  
Lipid Metabolism ◽  
Fatty Acid Synthase ◽  
Mrna Levels ◽  
Lipid Uptake ◽  
Marbling Score ◽  
Adipose Tissues ◽  
Korean Cattle ◽  
Subcutaneous Adipose

Abstract This study investigated effects of dietary glycerol supplementation on liver, muscle, and adipose gene expression related with gluconeogenesis and lipid metabolism and association of gene expression levels with marbling score in Korean cattle steers. Fourteen Korean cattle steers (average age 28.4 months; average body weight 733 kg) were equally assigned to two groups (0 and 5% glycerol supplementation). Glycerol was provided with glycerol (63%)-adsorbed ground wheat bran (37%, DM) by top dressing during roughage feeding. A concentrate (1.2% of body weight) and 1.0 kg of ryegrass were individually fed twice daily. After four months of study, steers were slaughtered, and marbling score was evaluated. Longissimus thoracis (LT) and subcutaneous adipose tissue at the 13th thoracic vertebra area and liver were collected and analyzed for mRNA levels by quantitative real-time PCR. Statistical significance was analyzed by analysis of variance. Correlations were analyzed using Pearson’s correlation analysis. Glycerol supplementation increased (P = 0.01) marbling score. In the LT, glycerol supplementation tended to increase (0.05 &lt; P ≤ 0.10) lipid uptake CD36 and lipoprotein lipase (LPL) mRNA levels. In subcutaneous adipose tissues, glycerol supplementation increased (P ≤ 0.05) LPL, adipogenic sterol regulatory element binding protein 1 (SREBP1), and lipogenic acetyl CoA carboxylase (ACC) mRNA levels and tended to increase (0.05 &lt; P &lt; 0.10) CD36, adipogenic peroxisome proliferator-activated gamma (PPARG), and lipogenic fatty acid synthase (FASN) expression. It did not affect (P &gt; 0.05) mRNA levels of hepatic gluconeogenesis genes. Marbling score showed significant positive correlations (0.57 &lt; r &lt; 0.68; P &lt; 0.05) with mRNA levels of several genes including LPL, PPARG, SREBP1, and ACC in adipose tissues, but not with any genes examined in the LT. Our study demonstrates that lipid uptake, adipogenesis and lipogenesis may mainly contribute to the increased marbling score by glycerol supplementation.

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