scholarly journals Comparison of Metabolic Network between Muscle and Intramuscular Adipose Tissues in Hanwoo Beef Cattle Using a Systems Biology Approach

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Hyun-Jeong Lee ◽  
Hye-Sun Park ◽  
Woonsu Kim ◽  
Duhak Yoon ◽  
Seongwon Seo
Keyword(s):  
Systems Biology ◽  
Beef Cattle ◽  
Metabolic Regulation ◽  
Bos Taurus ◽  
Adipose Tissues ◽  
Chemokine Signaling ◽  
Gene Network Analysis ◽  
Chemokine Signaling Pathway ◽  
Subcutaneous Adipose

The interrelationship between muscle and adipose tissues plays a major role in determining the quality of carcass traits. The objective of this study was to compare metabolic differences between muscle and intramuscular adipose (IMA) tissues in thelongissimus dorsi(LD) of Hanwoo (Bos taurus coreanae) using the RNA-seq technology and a systems biology approach. The LD sections between the 6th and 7th ribs were removed from nine (each of three cows, steers, and bulls) Hanwoo beef cattle (carcass weight of430.2±40.66 kg) immediately after slaughter. The total mRNA from muscle, IMA, and subcutaneous adipose and omental adipose tissues were isolated and sequenced. The reads that passed quality control were mapped onto the bovine reference genome (build bosTau6), and differentially expressed genes across tissues were identified. The KEGG pathway enrichment tests revealed the opposite direction of metabolic regulation between muscle and IMA. Metabolic gene network analysis clearly indicated that oxidative metabolism was upregulated in muscle and downregulated in IMA. Interestingly, pathways for regulating cell adhesion, structure, and integrity and chemokine signaling pathway were upregulated in IMA and downregulated in muscle. It is thus inferred that IMA may play an important role in the regulation of development and structure of the LD tissues and muscle/adipose communication.

Comparative evaluation of the meat quality of beef cattle breeds of Indian, African and European origins

1999 ◽  
Vol 69 (1) ◽  
pp. 135-142 ◽  
Author(s):  
C. Gazzola ◽  
C. J. O’Neill ◽  
J. E. Frisch
Keyword(s):  
Connective Tissue ◽  
Beef Cattle ◽  
Meat Quality ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Cooking Loss ◽  
Peak Force ◽  
Beef Herd ◽  
Beef Cattle Breeds

AbstractThe aim of this study was to rank diverse beef cattle genotypes for meat quality characteristics and to determine whether that ranking changed depending on the environment in which the animals were finished. Breed groups, ranging from 100% Bos indicus to 100% Bos taurus content, were derived from Indian zebu (Brahman), African zebu (Boran), British breeds (Hereford-Shorthorn), continental breeds (Charolais and Simmental) and Sanga (Tuli and Belmont Red). Heavy steers (>600 kg) were raised in a tropical environment and finished either on pasture or in a feedlot.For striploins (longissimus) from feedlot finished steers, cooking loss was greatest for zebu (Z) steaks, least for British (B) steaks (P < 0·001), and intermediate for the other breeds. For striploins from pasture finished steers and eye rounds (semitendinosus) from both pasture finished and feedlot finished steers, there were no breed differences in cooking loss.For both feedlot finished and pasture finished steers, striploin steaks from B steers were most tender, and Sanga (S) and zebu × continental cross (ZC) steaks were more tender than Z steaks. Warner-Bratzler initial yields suggested that the lower toughness of these breeds was due to lower myofibrillar toughness. Peak force minus initial yield suggested a smaller, opposite effect of increasing connective tissue toughness associated with increasing Bos taurus content. This breed effect on connective tissue toughness was more prominent in the eye round samples. In the feedlot finished steers, Z eye round was as tender as B eye round. In pasture finished steers, Z eye round had a peak force 1·3 kg lower than B in contrast to the results for striploin where Z was 1·2 kg higher than B. Sanga eye round followed the same trend as B relative to Z but to a lesser extent. From the feedlot, S eye round was more tender than Z eye round and from pasture, S eye round was the same as Z eye round. There was no evidence of heterosis f or any meat quality attribute.These results are best explained in terms of a model incorporating differential effects of breed on the myofibrillar and connective tissue components of toughness. The implications of this study are that the tenderness of grilling and roasting cuts of meat from the predominantly Brahman-based beef herd of northern Australia can be improved through crossbreeding with any of the taurine breeds studied.

Transcriptome analysis of subcutaneous adipose tissues in beef cattle using 3′ digital gene expression-tag profiling1

2012 ◽  
Vol 90 (1) ◽  
pp. 171-183 ◽  
Author(s):  
W. Jin ◽  
E. N. Olson ◽  
S. S. Moore ◽  
J. A. Basarab ◽  
U. Basu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Beef Cattle ◽  
Transcriptome Analysis ◽  
Digital Gene Expression ◽  
Adipose Tissues ◽  
Subcutaneous Adipose

scholarly journals The clinical significance of glutathione peroxidase 2 in glioblastoma multiforme

2021 ◽  
Vol 12 (1) ◽  
pp. 032-039
Author(s):  
Bangming Guo ◽  
Wenjuan Liao ◽  
Shusheng Wang
Keyword(s):  
Glioblastoma Multiforme ◽  
Glutathione Peroxidase ◽  
Mrna Expression ◽  
Signaling Pathway ◽  
Clinical Significance ◽  
Cancer Cell ◽  
Statistical Significance ◽  
Adult Brain ◽  
Chemokine Signaling ◽  
Chemokine Signaling Pathway

Abstract Background Glioblastoma multiforme (GBM) is the leading cause of death among adult brain cancer patients. Glutathione peroxidase 2 (GPX2), as a factor in oxidative stress, plays an important role in carcinogenesis. However, its role in GBM has not been well established. The study aimed to investigate the clinical significance of GPX2 with GBM prognosis. Methods Data of GBM and healthy individuals were retrospectively collected from oncomine, cancer cell line encyclopedia (CCLE), gene expression profiling interactive analysis (GEPIA), UALCAN, and Human Protein Atlas. GPX2 mRNA expression was first assessed across various cancer types in oncomine and cancer cell lines from CCLE. The mRNA expression of GPX2 was compared between normal and GBM tissues using GEPIA (normal = 207; GBM = 163) and UALCAN (normal = 5; GBM = 156). The GPX2 methylation was analyzed using data from UALCAN (normal = 2; GBM = 140). The prognostic value of GPX2 in GBM was explored in GEPIA and UALCAN using Kaplan–Meier method. STRING database was used to construct protein–protein interaction (PPI) network and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. Statistical significance was set as <0.05. Results The current study revealed no significant differences in GPX2 expression between normal and GBM from GEPIA data (P > 0.05) and UALCAN (P = 0.257). Patients with higher GPX2 intended to have a poorer prognosis (P = 0.0089). The KEGG pathways found that chemokine-signaling pathway were the more preferred. Conclusions The findings demonstrated that GPX2 might be a potential diagnosis and prognostic indicator for GBM. Chemokine-signaling pathway may be involved in GPX2 function.

scholarly journals Characterisation of intramuscular, intermuscular and subcutaneous adipose tissues in yearling bulls of different genetic groups

Meat Science ◽  
2007 ◽  
Vol 76 (4) ◽  
pp. 682-691 ◽  
Author(s):  
N. Aldai ◽  
A.I. Nájera ◽  
M.E.R. Dugan ◽  
R. Celaya ◽  
K. Osoro
Keyword(s):  
Adipose Tissues ◽  
Genetic Groups ◽  
Subcutaneous Adipose

Effect of feeding barley based diets on animal performance, carcass characteristics and meat quality of crossbred beef cattle with and without Wagyu genetics

1997 ◽  
Vol 77 (4) ◽  
pp. 655-662 ◽  
Author(s):  
P. S. Mir ◽  
D. R. C. Bailey ◽  
Z. Mir ◽  
S. D. M. Jones ◽  
T. Entz ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Meat Quality ◽  
Shear Force ◽  
Average Daily Gain ◽  
Carcass Characteristics ◽  
Barley Grain ◽  
Crossbred Cattle ◽  
Target Weight ◽  
Meat Samples

Growth performance, carcass characteristics and meat quality of European and British crossbred (EBC; no Wagyu genetics; 28 heifers and 30 steers) cattle were compared with crossbred cattle with 75% Wagyu genetics (WC; seven heifers and 14 steers) to determine the influence of Wagyu genetics on marbling grade of beef cattle fed barley-based diets in a factorial design experiment. Weaned calves (250 d average age) were fed, one of two diets (diet 1, 35% barley grain; diet 2, 40% hay cubes on DM basis, with barley silage, protein and vitamin/mineral premix) for 84 d and then fed diet 1 until they weighed 394 to 432 kg. All cattle were finished on an 80% (DM basis) rolled barley diet and slaughtered. Carcasses were graded and samples procured for meat quality and Warner–Bratzler shear force determination. Number of days on backgrounding diets to arrive at target weight (380 kg) was greater (P < 0.05) for the WC cattle, owing to relatively lower ADG, but days on the finishing diet were fewer for these cattle, compared with EBC cattle. Warm carcass yield (dressing percent) was greater (P < 0.05), but backfat depth was lower (P < 0.05) for WC cattle relative to that of EBC cattle, yet proportion of lean meat yield was similar. Eighty three percent of WC cattle carcasses had Canada AAA (small or more) marbling grade compared with 13% for EBC cattle carcasses. Mean shear force of meat samples from EBC and WC cattle was 4.2 and 3.8 kg, respectively. Results indicated that the extent of carcass marbling can be increased by incorporating Wagyu genetics but age at slaughter of WC cattle was 19 d greater than that of EBC cattle and carcass size was reduced. Key words: Average daily gain, carcass characteristics, European and British crossbred, feed to gain ratio, meat quality, Wagyu crossbred cattle

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