A tryptophan-enriched diet improves feed intake and growth performance of susceptible weanling pigs orally challenged with Escherichia coli K881

2009 ◽  
Vol 87 (1) ◽  
pp. 148-156 ◽  
Author(s):  
P. Trevisi ◽  
D. Melchior ◽  
M. Mazzoni ◽  
L. Casini ◽  
S. De Filippi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Growth Performance ◽  
Feed Intake ◽  
Weanling Pigs

scholarly journals 150 A bio-active, mineral-based feed additive improved growth performance and reduced severity of diarrhea in weanling pigs challenged with enterotoxigenic Escherichia coli

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 84-85
Author(s):  
Sue Sinn ◽  
Ran Song ◽  
Dana Beckler ◽  
Rob Musser ◽  
Kim Friesen
Keyword(s):  
Escherichia Coli ◽  
Growth Performance ◽  
Meta Analysis ◽  
Enterotoxigenic Escherichia Coli ◽  
Feed Additive ◽  
Post Inoculation ◽  
Adaptation Period ◽  
Weanling Pigs ◽  
Serum Samples ◽  
E Coli

Abstract A mineral-based feed additive, NutriQuest Protect™, was evaluated in five artificial Enterotoxigenic Escherichia coli (ETEC) challenge experiments to determine the effects on pig growth performance, fecal consistency and immune response. The five experiments were conducted following a similar procedure and utilized a total of 232 weanling pigs (19 d of age) assigned to one of three experimental treatments: non-challenged control (NC), challenged control (CC), and challenged pigs fed Protect at 4.0 g/kg (CP) with 36, 36, and 44 pens per treatment, respectively. Pharmacological ZnO or medications were not included in any diets. Pigs were allowed a 7-d adaptation period following weaning, orally inoculated with E. coli K88 or F18 on 0 d post-inoculation (dpi) and 1-dpi. Studies were concluded on 4-dpi. Pig BW and feed disappearance were measured on 0-dpi and 4-dpi. Serum samples were collected on 0 and 4-dpi to measure porcine proinflammatory cytokines. Fecal scores were measured daily over the challenge period. Data from the five experiments were compiled for meta-analysis using the MIXED procedure of SAS. The NC pigs had a greater ADG (0.09 vs. -0.01 kg/d, P = 0.002), ADFI (0.24 vs. 0.21 kg/d, P = 0.09), and final BW (6.8 vs. 6.5 kg, P < 0.05). Diarrhea frequency was significantly higher in CC pigs compared with pigs on CP and NC treatments (28.5 vs. 18.7 vs. 5.3%, P < 0.05). Results from the five experiments suggest that NutriQuest Protect™ improves growth performance and reduces inflammation and diarrhea in weaned pigs artificially challenged with E. coli K88 or F18.

scholarly journals Biological response of piglets challenged with Escherichia coli F4 (K88) when fed diets containing intestinal alkaline phosphatase

2021 ◽  
Author(s):  
Jansller Genova ◽  
Paulo Evaristo Rupolo ◽  
Antonio Diego Brandão Melo ◽  
Liliana Bury de Azevedo dos Santos ◽  
Geraldyne Nunes Wendt ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Alkaline Phosphatase ◽  
Growth Performance ◽  
Feed Intake ◽  
Average Daily Gain ◽  
Enterotoxigenic Escherichia Coli ◽  
Basic Solution ◽  
Intestinal Alkaline Phosphatase ◽  
Ph Values ◽  
Daily Gain

The aim of the study was to investigate the effect of intestinal alkaline phosphatase (IAP) added to diets on growth performance, diarrhoea incidence (DI), blood metabolites, relative organ weight, and intestinal morphometry of weaned piglets challenged with enterotoxigenic Escherichia coli F4 (K88). A total of 64 crossbred entire male piglets (25-day-old and 7.16 ± 0.28 kg body weight) were allocated into four treatments: control diet (CD<sup>–</sup>), CD<sup>–</sup> + antimicrobial growth promoter (AGP), CD<sup>–</sup> + 15 mg IAP/kg of diet and CD<sup>–</sup> + 30 mg IAP/kg of diet, with eight replications. At 15 days, all piglets were orally challenged with 6 ml of a solution containing K88 (10<sup>6</sup> colony forming units/ml). Microencapsulated IAP in acid solution showed 14.43% solubility and pH values of 1.69, 1.72, 1.51, and 1.52 at the different times measured (0.5 h, 1.0 h, 17.0 h, and 24 h); differently, IAP in basic solution had 4.10% solubility and pH values increased (5.95, 6.10, 6.32 and 6.63) according to the different times, respectively. On days 25–35, piglets that received 30 mg IAP and CD<sup>–</sup> showed a better feed conversion ratio (P = 0.075) compared to those fed 15 mg IAP. Piglets that consumed 30 mg IAP or CD<sup>–</sup> had higher (P = 0.004) average daily gain on days 35–44. On days 35–44, the piglet average daily feed intake was lower (P = 0.033) with 15 mg IAP compared to AGP. In the entire period, piglets fed 15 mg IAP showed a reduction in average daily gain (P = 0.040) and average daily feed intake (P = 0.092). Piglets on 30 mg IAP showed an improvement (P ≤ 0.05) in DI in the pre-and post-challenge periods. The relative spleen weight of the piglet increased (P = 0.043) in response to 30 mg IAP. Overall, the addition of 30 mg IAP to diets improves the growth performance, attenuates DI, and promotes an increase in spleen relative weight to maintain the healthy state of piglets.  

scholarly journals Effects of an F18 enterotoxigenic Escherichia coli challenge on growth performance, immunological status, and gastrointestinal structure of weaned pigs and the potential protective effect of direct-fed microbial blends

2020 ◽  
Vol 98 (5) ◽  
Author(s):  
Spenser L Becker ◽  
Qingyun Li ◽  
Eric R Burrough ◽  
Danielle Kenne ◽  
Orhan Sahin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Growth Performance ◽  
Feed Intake ◽  
Average Daily Gain ◽  
Immunoglobulin A ◽  
Mrna Abundance ◽  
Enterotoxigenic Escherichia Coli ◽  
Protective Effects ◽  
Intestinal Function ◽  
The Impact

Abstract The objective of this experiment was to investigate the impact of an F18 enterotoxigenic Escherichia coli (ETEC) challenge on growth performance, aspects of intestinal function, and selected immune responses of piglets, as well as to evaluate potential protective effects of direct-fed microbial (DFM) blends. Seventy-two weaned piglets (6.4 ± 0.2 kg body weight [BW]; ~21 d of age) were assigned to one of four treatments: 1) NC: Nonchallenged (n = 10), 2) positive challenged control (PC): F18 ETEC-challenged (n = 10), 3) PC + DFM1 (n = 8; three strains of Bacillus amyloliquefaciens; 7.5 × 105 colony-forming units [cfu]/g), or 4) PC + DFM2 (n=8; 2 strains of B. amyloliquefaciens and one strain of Bacillus subtilis; 1.5 × 105 cfu/g). Feed intake and BW were recorded on day 0, 7, and 17. Pigs were sham-infected either with 6 mL phosphate-buffered saline or inoculated with 6 mL F18 ETEC (~1.9 × 109 cfu/mL) on day 7 (0 d postinoculation [dpi]). All ETEC-challenged pigs were confirmed to be genetically susceptible to F18. Pigs had ad libitum access to feed and water throughout the 17-d trial. Fecal scores were visually ranked and rectal temperatures were recorded daily. To evaluate ETEC shedding, fecal swabs were collected on dpi 0, 1, 2, 3, 5, 7, and 10. Blood samples were collected on dpi 0, 1, 2, 4, 7, and 10. Ileal tissues were collected at necropsy on dpi 10. All challenged treatments had lower final BW, decreased average daily gain (ADG), and average daily feed intake (ADFI) during the 10-d postchallenge period (P &lt; 0.01). The DFM2 treatment increased E. coli shedding on dpi 2 and decreased iton dpi 7 (P &lt; 0.05) compared with the PC. Rectal temperature decreased across all challenged treatments (P &lt; 0.01). Ileal mRNA abundance of occludin (OCLN) and zonula occludens-1 (ZO-1) decreased in PC and DFM1 compared with NC (P &lt; 0.05). Pigs fed DFM2 had intermediate ileal mRNA abundance of OCLN and increased ZO-1 mRNA compared with pigs in PC (P &lt; 0.05). Interleukin 8 (IL-8) increased in the plasma of PC and DFM2 on dpi 2 compared with NC (P &lt; 0.05). Mucosal IL-8 increased in PC compared with NC (P &lt; 0.05). All challenged treatments tended to have elevated tumor necrosis factor-α (TNF-α) mRNA abundance compared with NC (P &lt; 0.10). Challenged pigs had reduced secretory immunoglobulin A and villus height compared with NC pigs (P &lt; 0.05). The impact of an ETEC challenge on intestinal function and the immune system has been revealed, information critical to developing improved treatment regimes.

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