In vitro gas production profiles to estimate extent and effective first-order rate of neutral detergent fiber digestion in the rumen

2008 ◽  
Vol 86 (3) ◽  
pp. 651-659 ◽  
Author(s):  
P. Huhtanen ◽  
A. Seppälä ◽  
M. Ots ◽  
S. Ahvenjärvi ◽  
M. Rinne
Keyword(s):  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Fiber Digestion ◽  
First Order ◽  
Order Rate ◽  
In Vitro Gas Production

scholarly journals Comparison Effects of Ruminal Crabtree-Negative Yeasts and Crabtree-Positive Yeasts for Improving Ensiled Rice Straw Quality and Ruminal Digestion Using In Vitro Gas Production

2020 ◽  
Vol 6 (3) ◽  
pp. 109 ◽  
Author(s):  
Chanon Suntara ◽  
Anusorn Cherdthong ◽  
Suthipong Uriyapongson ◽  
Metha Wanapat ◽  
Pin Chanjula
Keyword(s):  
Rice Straw ◽  
Dry Matter ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Control Group ◽  
In Vitro Digestibility ◽  
Pichia Kudriavzevii ◽  
Yeast Strains ◽  
In Vitro Gas Production

The objective of this study was to compare the effects of Crabtree-negative ruminal yeast and Crabtree-positive yeast in ensiled rice straw (RS) on the ensilage quality, nutritive value, and microorganism composition, including the evaluation of the ensiled RS using the in vitro gas production technique. The experiment was conducted in a 4 × 3 factorial arrangement in a randomized complete design. Factor A was yeast species with no inoculant, Crabtree-negative yeasts (Pichia kudriavzevii KKU20 and Candida tropicalis KKU20), and Crabtree-positive yeast (Saccharomyces cerevisae), whereas factor B was ensilage times (7, 14, and 21 days). The rate of growth was revealed to be lower in Crabtree-positive yeasts than the other Crabtree-negative yeast strains (p < 0.01). RS ensiled with S. cerevisiae showed decreased dry matter (DM) content by 9.0% when compared to the sample without a yeast inoculant. In addition, organic matter (OM) content was greater (p < 0.01) for P. kudriavzevii KKU20 than C. tropicalis KKU20 and without an inoculant. Neutral detergent fiber (NDF) content was significantly decreased (p < 0.01) by yeast inoculants by about 2.75% when compared to the control group. Lactic acid bacteria (LAB) and aerobic bacteria were low (p < 0.05) when yeasts were added. However, no interaction was found between yeast and ensilage times on the quality of ensiled RS (p > 0.05). The P. kudriavzevii KKU20 addition was associated with the highest value (p < 0.01) of gas produced—an insoluble fraction (b), potential extent of gas production (a + b), and cumulative gas production at 96 h—when compared with S. cerevisiae or the control group. The highest in vitro dry matter digestibility and in vitro neutral detergent fiber digestibility was observed in RS ensiled with P. kudriavzevii KKU20 for 14 days (p < 0.01, p < 0.05). The maximum total volatile fatty acids (VFAs) at 4 and 8 h of incubation and the mean value were observed in RS ensiled with P. kudriavzevii KKU20 (p < 0.01). RS ensiled with all yeast strains showed an increased propionate concentration at 8 h (p < 0.01). In conclusion, ensiling RS with isolated Crabtree-negative ruminal yeasts could benefit feed digestion and in vitro gas production more than Crabtree-positive yeast does. P. kudriavzevii KKU20, an isolated Crabtree-negative ruminal yeast used to treat RS, had the highest potential for increasing cumulative gas production and enhancing in vitro digestibility.

scholarly journals Do fibrolytic, proteolytic and amylolytic enzymes influence the in vitro fermentation characteristics of forage?

2018 ◽  
Vol 39 (3) ◽  
pp. 1143
Author(s):  
Lucien Bissi da Freiria ◽  
Joanis Tilemahos Zervoudakis ◽  
Nelcino Franciso de Paula ◽  
Luciano Da Silva Cabral ◽  
Luis Orlindo Tedeschi ◽  
...  
Keyword(s):  
Lag Time ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Liquid Volume ◽  
Brachiaria Brizantha ◽  
In Vitro Fermentation ◽  
Time Points ◽  
In Vitro Gas Production ◽  
Fermentation Profile

The effects of increasing doses of three exogenous enzymes preparations with fibrolytic activity (FIB - 0, 0.6, 1.2, 1.8, and 2.4 mg mL-1liquid volume incubated), amylolytic activity (AMZ - 0, 0.05, 0.10, 0.15, and 0.20 mg mL-1liquid volume incubated), and proteolytic activity (PRO - 0, 0.05, 0.10, 0.15, and 0.20 mg mL-1 liquid volume incubated ) on gas production (GP), kinetic parameters, and fermentation profile of Brachiaria brizantha cv. Marandu were evaluated using the in vitro gas production technique. Ruminal liquid was obtained from two rumen-cannulated Santa Inês sheep maintained on pasture. Accumulated gas production was measured during 96 hours of incubation, measured at 18 different time points. The determined parameters were pH, asymptotic gas production (mL g-1), rate of gas production (h-1), lag time (h), organic matter digestibility (OMD, g g-1 DM), metabolizable energy (ME, MJ kg-1 DM), and neutral detergent fiber digestibility (NDFD, mg g-1 DM). Increasing the FIB dose linearly increased (P < 0.05) the asymptotic gas production. However, the rate of gas production and the lag time showed linear decreases (P < 0.05). Addition of FIB also linearly increased (P < 0.05) the GP at all incubation times, as well as the OMD, NDFD and ME. Addition of AMZ linearly increased (P < 0.05) the asymptotic gas production, but GP linear increased (P < 0.05) only at the 6-hour and 12-hour time points. The rate of gas production and the lag time decreased linearly (P < 0.05) in response to increasing AMZ addition. Inclusion of PRO did not affect (P > 0.05) asymptotic gas production, but there was quadratic effect (P < 0.05) on the rate of gas production, the lag time, and the GP at the 6-hour and 12-hour time points. The OMD, NDFD and ME were not affected by PRO addition. Thus, fibrolytic, amylolytic and proteolytic enzymes are effective in reducing the lag time and increasing the in vitro gas production from Brachiaria Brizantha cv. Marandu forage, and fibrolytic enzymes improve the in vitro fermentation profile.

scholarly journals Can ruminal inoculum from slaughtered cattle replace inoculum from cannulated cattle for feed evaluation research?

2018 ◽  
Vol 39 (5) ◽  
pp. 2133
Author(s):  
Henry Daniel Ruiz Alba ◽  
Ronaldo Lopes Oliveira ◽  
Silvana Teixeira de Carvalho ◽  
Luís Carlos Vinhas Ítavo ◽  
Ossival Lolato Ribeiro ◽  
...  
Keyword(s):  
Evaluation Research ◽  
Invasive Procedure ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Dry Matter Digestibility ◽  
In Vitro Gas Production ◽  
Nitrogen Concentrations ◽  
Different Levels

The objective was to test the hypothesis that ruminal inoculum obtained from slaughtered cattle can replace inoculum from cannulated cattle in trials evaluating animal feedstuffs through in vitro gas production and digestibility. Five adult Holstein × Zebu steers with ruminal cannula were used to collect and compare rumen liquid from in vivo and slaughtered animals. In vitro dry matter digestibility (IVDMD), in vitro neutral detergent fiber digestibility (IVNDFD) and ammoniacal nitrogen concentrations did not differ between inoculum sources (slaughtered × cannulated) for all byproducts and levels tested. Total in vitro gas production in the ruminal inoculum of cannulated animals was greater (P < 0.001) than slaughtered cattle for different levels of licuri cake. However, the greatest total concentrations of in vitro gases for slaughtered animals were observed when evaluating different levels of crude glycerin (P < 0.001). No differences were observed for diets containing castor bean meal (P >0.05). Thus, the ruminal inoculum obtained from the ruminal contents of slaughtered cattle can replace the use of fistulated animals and is a viable alternative to digestibility analysis. This approach is ethically more correct because it alleviates the suffering of animals by avoiding an invasive procedure.

scholarly journals The potential of tropical grass as a feed in ruminant by using an in vitro gas production

2018 ◽  
Vol 197 ◽  
pp. 06005
Author(s):  
Nevyani Asikin ◽  
Anuraga Jayanegara ◽  
Muhamad Ridla ◽  
Anjas Asmara Samsudin
Keyword(s):  
Animal Feed ◽  
Block Design ◽  
In Vitro Study ◽  
Proximate Analysis ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
In Vitro Gas Production ◽  
Significant Difference ◽  
Tropical Grass

Pennisetum purpoides is one of the most widely cultivated tropical forages and it is often used as animal feed in Indonesia. However, grass feeding could not fulfill the need of nutrients if served as single feed because it contained of high crude fiber and low protein. In terms of nutritional adequacy, animal feed should contain complete nutritional value in order to achieve an optimum productivity. This experiment aimed to elucidate the potential of tropical grass as a feed in ruminant by using an in vitro gas production technicque. The grasses were determined for proximate analysis, Van Soest fiber fractions (neutral detergent fiber, acid detergent fiber and acid detergent lignin), and in vitro rumen fermentation parameters. This study was conducted from January until April 2018 at Nutrition Laboratory, Department of Animal Science, Faculty of Agriculture, University Putra Malaysia, Serdang Selangor Darul Ehsan, Malaysia. This study used a randomized block design with 3 replications. There were three treatments tested in this study, i.e. (1) Pennisetum purpoides (T1), (2) Setaria splendida (T2), (3) Setaria anceps (T3). Parameters measured in the in vitro study is total gas production. Data were tested using analysis of variance (ANOVA) and continued with Duncan test if there was a significant difference among treatments. The data showed that gas production is not significantly different (P>0,05).

scholarly journals Addition of Lactic Acid Bacteria Can Promote the Quality and Feeding Value of Broussonetia papyrifera (Paper Mulberry) Silage

Fermentation ◽  
2022 ◽  
Vol 8 (1) ◽  
pp. 25
Author(s):  
Wen-Tao Sun ◽  
Yuan Huang ◽  
Chang-Rong Wu ◽  
Chao Peng ◽  
Yu-Long Zheng ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Southwest China ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Control Group ◽  
Broussonetia Papyrifera ◽  
In Vitro Gas Production ◽  
Feeding Value

In this study, the influence of two lactic acid bacteria (LAB) strains [Lactobacillus rhamnosus BDy (LR-BDy) and Lactobacillus buchneri TSy (LB-TSy)] selected from Southwest China on the fermentation characteristics and in vitro gas production of Broussonetia papyrifera (paper mulberry) silage were experimentally explored. The experimental groups were a control group (C), an LB-TSy treatment (LB), an LR-BDy treatment (LR), and an LR-BDy + LB-TSy hybrid group (LR × LB). After the LAB were added, the pH value of paper mulberry silage significantly declined (p < 0.05), and the crude protein content was effectively preserved (p < 0.05). However, no significant changes were found in the levels of neutral detergent fiber, acid detergent fiber, and crude ash (p > 0.05). The lactic acid content in paper mulberry silage was evidently increased (p < 0.05). The in vitro gas production in the LR at 36, 48, and 72 h were markedly higher than that in the other treatments (p < 0.05). Owing to the addition of LAB, the microbial diversity in paper mulberry silage was reduced, while the relative bacterial abundance of Lactobacillus was enhanced. Hence, the addition of LAB selected from the warm and humid region in Southwest China can improve the quality of paper mulberry silage and elevate its feeding value in this region.

scholarly journals 19. Improving the Prediction of Methane Production Determined by in Vitro Gas Production Technique for Ruminants

2016 ◽  
Vol 16 (2) ◽  
pp. 565-584
Author(s):  
Chung-Nan Chen ◽  
Tzu-Tai Lee ◽  
Bi Yu
Keyword(s):  
Chemical Composition ◽  
Starch Content ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Chemical Compositions ◽  
Production Technique ◽  
In Vitro Gas Production ◽  
True Digestibility ◽  
Protein Rich

Abstract Twelve feedstuffs (cereals, fibrous byproducts, protein-rich byproducts and forages) were determined for methane (CH4) production by the in vitro gas production technique (IVGPT) and were correlated with their chemical compositions to predict enteric CH4 originating from these feedstuffs in ruminants. Corn, soybean hull, soybean meal and corn silage generated the highest CH4 production from their respective categories. The average CH4 production of fibrous byproducts (44.6 ml/g DM incubated) was significantly higher than that of cereals (40.3 ml/g DM incubated), forages (33.3 ml/g DM incubated) and protein-rich byproducts (31.0 ml/g DM incubated) after the 48-h incubation (P≤0.05). The highest average total volatile fatty acid (VFA) concentration was determined in cereals (53.6 mM). The acetate to propionate ratio was significantly lower in cereals when compared with other categories of feedstuff (P≤0.05). The correlation analysis showed that in vitro true digestibility (IVTD) positively correlated with the CH4 production in all four categories of feedstuffs (P≤0.05). The neutral detergent fiber (NDF) and acid detergent fiber (ADF) content positively correlated with CH4 production in every category of feedstuffs except cereals. The starch content negatively correlated with CH4 production for fibrous and protein-rich byproducts (P≤0.05), but it positively correlated with CH4 production for forages (P≤0.05). The CH4 production was predicted more accurately by the equations proposed for each category (R2=0.944, 0.876, 0.942 and 0.915 for cereals, fibrous and protein-rich byproducts and forages, respectively) than for the unclassified feedstuffs (R2=0.715). In conclusion, the contribution of individual chemical composition to CH4 production differed depending on the category of feedstuffs. The precision of CH4 prediction could be substantially improved by classifying feedstuffs into categories according to their chemical composition, and selecting the appropriate predictors for each category. Information about the CH4 output of these feedstuffs will be useful in formulating low CH4-producing diets for ruminants.

scholarly journals Farklı Maltlık Arpa Çeşitlerinin Besin Değerlerinin In Vitro Gaz Üretim Tekniği Kullanılarak Tespiti

2017 ◽  
Vol 5 (10) ◽  
pp. 1216 ◽  
Author(s):  
Behlül Sevim ◽  
Tugay Ayaşan ◽  
İsmail Ülger ◽  
Şerife Ergül ◽  
Sait Aykanat ◽  
...  
Keyword(s):  
Crude Protein ◽  
Nutritive Value ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Net Energy ◽  
A Value ◽  
In Vitro Gas Production ◽  
Metabolisable Energy ◽  
Malt Barley

This study was conducted to determine the nutritive value of malt barley varieties using in vitro gas production technique and in vitro methane production. Atılır, Durusu and Fırat were used as three malt barley varieties. As a result of this study, between the varieties in terms of dry matter (DM), crude ash (CA), crude protein (CP), crude fat (CF), neutral detergent fiber (NDF) and hemicellulose (HEM) were found significantly important but differences between the varieties in terms of ADF were not found significant. The gas production rate of malt barley varieties ranged from 64.00 to 72.50 ml/200 mg DM. The metabolisable energy (ME) and net energy lactation (NEL) contents of malt barley varieties ranged from 11.75 to 12.86 MJ/kg DM and 7.16 to 7.98 MJ/kg DM respectively. The highest methane (CH4) content was obtained from the variety of Fırat with a value of 13.34 ml/200 mg DM, whereas the lowest value was obtained from the Atılır variety with a value of 12.16 ml/200 mg DM.

scholarly journals Effect of using different levels of cassava meal in a concentrate cassava peel diet on chemical composition, in vitro gas production, and rumen fermentation

2021 ◽  
Vol 888 (1) ◽  
pp. 012053
Author(s):  
A D Saputra ◽  
Kusmartono ◽  
Mashudi ◽  
P H Ndaru
Keyword(s):  
Organic Matter ◽  
Chemical Composition ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Cassava Leaves ◽  
In Vitro Gas Production ◽  
Cassava Peel ◽  
Different Levels

Abstract This study was designed to evaluate effects of using different levels of cassava meal in a concentrate cassava peel diet on chemical composition, in vitro gas production (IVGP) and rumen fermentation. The treatments applied were: A=cassava peel (20%)+ cassava meal (70%)+cassava leaves (5%)+moringa leaves (5%); B=cassava peel (20%)+ cassava meal (60%)+cassava leaves (10%)+moringa leaves (10%); C=cassava peel (20%)+ cassava meal (50%)+cassava leaves (15%)+moringa leaves (15%) cassava meal; D=cassava peel (20%)+ cassava meal (40%)+cassava leaves (20%)+moringa leaves (20%); E=cassava peel (20%)+ cassava meal (30%)+cassava leaves (25%)+moringa leaves (25%) with 3 replications arranged in a Randomized Block Design (RBD). The result showed that the increasing levels of cassava meal in the ration significantly increased organic matter (OM) and nitrogen-free extract (NFE) content (P<0.05), but reduced crude protein (CP), ether extract (EE), crude fiber (CF), neutral detergent fiber (NDF), and acid detergent fiber (ADF) contents. Similarly, a significant increased (P<0.01) were found in values of cumulative in vitro gas production, dry matter and organic matter digestibility, but decreased NH3 concentration (P<0.05) due to the increased of cassava meal. It is concluded that increasing levels of cassava meal in concentrate has led to higher OM content of the ration and being available for rumen fermentation.

scholarly journals Agronomic Characteristics, Chemical Composition and In vitro Gas Production of Sugarcane Cultivars (Saccharum spp.) for Feeding Ruminants

2019 ◽  
pp. 1-8
Author(s):  
Rafael Henrique Pereira Dos Reis ◽  
Joadil Gonçalves de Abreu ◽  
Roberto Giolo de Almeida ◽  
Luciano Da Silva Cabral ◽  
Carlos Eduardo Avelino Cabral ◽  
...  
Keyword(s):  
Organic Matter ◽  
Dry Matter ◽  
Latency Period ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
In Vitro Digestibility ◽  
Net Energy ◽  
Saccharum Spp ◽  
In Vitro Gas Production

The aim of this work was to evaluate the agronomic characteristics, chemical composition and in vitro gas production of sugarcane (Saccharum spp.) cultivars used in ruminant feed. The experimental design was a randomized block containing seven treatments and three replications totaling 21 plots. The cultivars RB835436, RB925211, RB925345, RB937570, RB945961, RB955970 e SP91-1049 was evaluated for dry matter, dry matter yield, stalk percentage, stalk tons per hectare, plant height, stalk diameter, number of tillers per linear meter, mass per stem, soluble solids content (BRIX), NDF/BRIX ratio and tons of sucrose/ha, total digestible nutrients, digestible energy, metabolizable energy, net energy and in vitro digestibility of organic matter, crude protein, neutral detergent fiber (NDF) and acid detergent, of digestion and In vitro gas production of non-fibrous carbohydrates, latency time, digestion rate and gas production of fibrous carbohydrates, and in vitro digestibility of organic matter. There was a significant difference between the cultivars regarding the neutral detergent fiber content, in vitro digestibility of organic matter, total digestible nutrients, digestible energy, net energy, degradation rates of fibrous and non-fibrous carbohydrates and latency period. There was a negative correlation between stem percentage and NDF/BRIX and positive correlation between in vitro digestibility of organic matter and total digestible nutrients. The results were submitted to analysis of variance and mean test by Scott-Knott and Pearson's correlation analysis. The statistical program used was SAEG 2000. The RB835486 variety was superior to the other cultivars, as it presented 93.28% of stem, 57.5% of in vitro digestibility of organic matter, NDF / BRIX ratio of 2.68, 43.78% NDF, latency period 2.86h and fibrous carbohydrate degradation rate of 2.26% per hour. Therefore, this cultivar was better indicated for animal feeding between May and July in the State of Mato Grosso, Brazil.

Kinetics of fiber digestion from in vitro gas production

1994 ◽  
Vol 72 (11) ◽  
pp. 2980-2991 ◽  
Author(s):  
P. Schofield ◽  
R. E. Pitt ◽  
A. N. Pell
Keyword(s):  
Gas Production ◽  
Fiber Digestion ◽  
In Vitro Gas Production ◽  
Kinetics Of
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