255 Using environmental swabbing to quantify the effectiveness of chemical disinfectant to reduce the quantity of porcine epidemic diarrhea virus RNA on feed manufacturing surfaces

2017 ◽  
Vol 95 (suppl_2) ◽  
pp. 123-123
Author(s):  
M. B. Muckey ◽  
R. A. Cochrane ◽  
J. C. Woodworth ◽  
S. S. Dritz ◽  
C. R. Stark ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Diarrhea Virus ◽  
Feed Manufacturing ◽  
Virus Rna ◽  
Porcine Epidemic Diarrhea ◽  
Chemical Disinfectant

scholarly journals Using environmental sampling to evaluate the effectiveness of decontamination methods to reduce detection of porcine epidemic diarrhea virus RNA on feed manufacturing surfaces

2021 ◽  
Author(s):  
Mary B Muckey ◽  
Cassandra K Jones ◽  
Jason C Woodworth ◽  
Chad B Paulk ◽  
Steve S Dritz ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Sodium Hypochlorite ◽  
Porcine Epidemic Diarrhea Virus ◽  
Diarrhea Virus ◽  
Feed Manufacturing ◽  
Ct Value ◽  
Reverse Transcription Pcr ◽  
Virus Rna ◽  
Biological Hazard ◽  
Porcine Epidemic Diarrhea

Abstract Porcine epidemic diarrhea virus (PEDV) is a possible biological hazard in feed mills. If the virus enters a feed mill, it becomes widely distributed and is difficult to decontaminate from both feed contact and non-feed contact surfaces. The objective of this study was to evaluate a variety of liquid and dry decontamination treatments that could be used to reduce the amount of PEDV found on feed manufacturing surfaces. This experiment was designed as a 5 × 10 factorial with 5 different feed manufacturing surfaces and 10 decontamination treatments with 3 replicates of each combination. Surfaces included stainless steel, solid polyethylene, woven polypropylene tote bag, rubber, and sealed concrete coupons. One mL (1×10 5 TCID50/mL) of stock PEDV was applied to each surface and allowed to dry completely for 60 min. Next, for decontamination requiring surface application, the application was performed and allowed 15 min contact time. The quantity of PEDV RNA was determined using quantitative reverse transcription PCR. A decontamination treatment × surface interaction was observed (P < 0.0001), indicating the efficacy of treatment is dependent upon the surface in which it is applied. Within the cement surfaces, the sodium hypochlorite resulted in the greatest (P < 0.05) cycle threshold (Ct) value, followed by formaldehyde which had a greater (P < 0.05) Ct value compared to remaining treatments. Within polyethylene, rubber, and stainless steel surfaces, the formaldehyde treated surfaces had the greatest Ct values (P < 0.05), followed by the sodium hypochlorite treatment, with other treatments all having lower Ct values (P < 0.05). For the woven polyethylene surfaces, the formaldehyde and sodium hypochlorite treatments had greater Ct values compared to all other treatments (P < 0.05). Additional research is necessary to identify the role of decontamination treatment on PEDV infectivity and develop methods for decontamination of feed manufacturing facilities.

scholarly journals 163 Utilizing feed sequencing to decrease the risk of porcine epidemic diarrhea virus (PEDV) cross-contamination during feed manufacturing

2016 ◽  
Vol 94 (suppl_2) ◽  
pp. 76-76 ◽  
Author(s):  
L. L. Schumacher ◽  
R. A. Cochrane ◽  
J. C. Woodworth ◽  
A. R. Huss ◽  
C. R. Stark ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Cross Contamination ◽  
Diarrhea Virus ◽  
Feed Manufacturing ◽  
Porcine Epidemic Diarrhea

scholarly journals Elimination of Porcine Epidemic Diarrhea Virus in an Animal Feed Manufacturing Facility

PLoS ONE ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. e0169612 ◽  
Author(s):  
Anne R. Huss ◽  
Loni L. Schumacher ◽  
Roger A. Cochrane ◽  
Elizabeth Poulsen ◽  
Jianfa Bai ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Animal Feed ◽  
Manufacturing Facility ◽  
Diarrhea Virus ◽  
Feed Manufacturing ◽  
Porcine Epidemic Diarrhea

scholarly journals Porcine Epidemic Diarrhea Virus RNA Present in Commercial Spray-Dried Porcine Plasma Is Not Infectious to Naïve Pigs

PLoS ONE ◽  
2014 ◽  
Vol 9 (8) ◽  
pp. e104766 ◽  
Author(s):  
Tanja Opriessnig ◽  
Chao-Ting Xiao ◽  
Priscilla F. Gerber ◽  
Jianqiang Zhang ◽  
Patrick G. Halbur
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Diarrhea Virus ◽  
Virus Rna ◽  
Porcine Epidemic Diarrhea ◽  
Spray Dried

scholarly journals Identifying outbreaks of Porcine Epidemic Diarrhea virus through animal movements and spatial neighborhoods

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Gustavo Machado ◽  
Carles Vilalta ◽  
Mariana Recamonde-Mendoza ◽  
Cesar Corzo ◽  
Montserrat Torremorell ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Animal Movements ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea
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