scholarly journals Growth of tobacco seedlings and plantlets in vitro as affected by in vitro environment.

1990 ◽  
Vol 28 (2) ◽  
pp. 31-39 ◽  
Author(s):  
Toyoki KOZAI ◽  
Akiko TAKAZAWA ◽  
Ichiro WATANABE ◽  
Jiro SUGI
Keyword(s):  
In Vitro Environment ◽  
Tobacco Seedlings

Microfluidic technologies as platforms for performing quantitative cellular analyses in an in vitro environment

The Analyst ◽  
2006 ◽  
Vol 131 (11) ◽  
pp. 1197 ◽  
Author(s):  
R. Scott Martin ◽  
Paul D. Root ◽  
Dana M. Spence
Keyword(s):  
In Vitro Environment

Controlled In Vitro Environment for Stage II Micropropagation of Chrysanthemum

1992 ◽  
Vol 35 (3) ◽  
pp. 1079-1083 ◽  
Author(s):  
J. L. Cuello ◽  
P. N. Walker ◽  
C. W. Heuser
Keyword(s):  
Stage Ii ◽  
In Vitro Environment

Pseudomonas solanacearum: virulence of biochemical mutants

1974 ◽  
Vol 20 (4) ◽  
pp. 519-529 ◽  
Author(s):  
D. L. Coplin ◽  
Luis Sequeira ◽  
R. S. Hanson
Keyword(s):  
Amino Acids ◽  
Xylem Sap ◽  
Systemic Infection ◽  
Maximum Growth ◽  
Population Doubling ◽  
Pseudomonas Solanacearum ◽  
Amino Acid Requirement ◽  
Mutant Strains ◽  
Tobacco Seedlings

The virulence of tryptophan auxotrophs (Trp−) of Pseudomonas solanacearum K60 was significantly lower than that of the wild type when inoculated on tobacco (cult. "Bottom Special") and tomato (cult. "Bonny Best"). These auxotrophs persisted at the site of inoculation for several weeks, but did not cause systemic infection. Auxotrophs for methionine (Met−) and leucine (Leu−) biosynthesis, however, were virulent on tobacco and weakly virulent on tomato. In tobacco leaves infiltrated with suspensions of Trp−, Met−, and Leu− mutant strains and strain K60, the population doubling times of each isolate were 16.0, 4.3, 4.2, and 2.3 h respectively. The amino acid requirement of each auxotroph was determined in vitro and compared with levels of these amino acids in xylem sap collected from decapitated plants. Tobacco sap contained sufficient concentrations of required amino acids to support 0, 53, and 54% of maximum growth in vitro of the Trp−, Met−, and Leu− mutant strains, respectively. Tryptophan, methionine, and leucine levels in tomato sap supported 0, 3, and 13% maximum growth of the respective strains in vitro. The relative concentrations of methionine and leucine in xylem sap were sufficiently different to explain the susceptibility of tobacco and resistance of tomato to these auxotrophs. The virulence of tryptophan auxotrophs was restored by genetic transformation to prototrophy and by supplying tryptophan to inoculated tobacco seedlings.

scholarly journals Influence of capsaicin on inflammatory cytokines induced by lipopolysaccharide in myoblast cells under in vitro environment

2017 ◽  
Vol 13 (49) ◽  
pp. 26 ◽  
Author(s):  
Touseef Amna ◽  
Inho Hwang ◽  
Ke Shang ◽  
Musarat Amina ◽  
NawalM Al-Musayeib ◽  
...  
Keyword(s):  
Inflammatory Cytokines ◽  
In Vitro Environment ◽  
Myoblast Cells

The In Vitro Environment and its Control in Micropropagation

1992 ◽  
pp. 247-282 ◽  
Author(s):  
T. Kozai ◽  
K. Fujiwara ◽  
M. Hayashi ◽  
J. Aitken-Christie
Keyword(s):  
In Vitro Environment
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