scholarly journals Studies on biological treatment of mechanical pulps. (II). Effect of depression of color reversion in TMP by treatment of crude enzyme solution from Pleurotus ostreatus.

1990 ◽  
Vol 44 (4) ◽  
pp. 487-495
Author(s):  
Sanro Tachibana ◽  
Tomoaki Koyanagi ◽  
Masashi Sumimoto
Keyword(s):  
Pleurotus Ostreatus ◽  
Biological Treatment ◽  
Crude Enzyme ◽  
Color Reversion ◽  
Enzyme Solution ◽  
Crude Enzyme Solution ◽  
Mechanical Pulps

scholarly journals Preparation and immobilization Glucoamylase and Pectinase by CLEA method

2014 ◽  
Vol 17 (2) ◽  
pp. 45-51
Author(s):  
Giang Thi Linh Tran ◽  
Oanh Ngoc Huynh
Keyword(s):  
Aspergillus Niger ◽  
Enzyme Preparation ◽  
Immobilized Enzyme ◽  
Solution Culture ◽  
Crude Enzyme ◽  
Cross Linking ◽  
Solid Culture ◽  
Enzyme Solution ◽  
Crude Enzyme Solution ◽  
Semi Solid

CLEA method (cross-linking enzyme aggregates) combines enzyme preparation and immobilization from solution culture into the same step. In this study, we applied CLEA method to immobilize two enzymes, glucoamylase and pectinase, from crude enzyme solution obtained from semi-solid culture of Aspergillus niger. The results showed that: In the same immobilized conditions (glucoamylase: 7% glutaraldehyde, 5°C, 2 hours; pectinase: 10% glutaraldehyde, 5°C, 2 hours), the immobilized enzyme from crude enzyme solution, has the abilities to be reused and activation stability under the influences of pH and temperature higher than immobilized commercial enzyme respectively.

scholarly journals Studies on Biological Treatment of Mechanical Pulps VI. Effect of carbonyl groups in mechanical pulps on depression of color reversion of mechanical pulps.

1993 ◽  
Vol 47 (2) ◽  
pp. 273-284 ◽  
Author(s):  
Sanro Tachibana ◽  
Yukio Adachi ◽  
Katsumi Ohkubo ◽  
Kazutaka Itoh ◽  
Masashi Sumimoto
Keyword(s):  
Biological Treatment ◽  
Carbonyl Groups ◽  
Color Reversion ◽  
Mechanical Pulps

scholarly journals Studies on biological treatment of mechanical pulps. (I). Screening of wood-rot fungi depressing color reversion of mechanical pulps.

1989 ◽  
Vol 43 (12) ◽  
pp. 1298-1305
Author(s):  
Sanro Tachibana ◽  
Yoshitsugu Honda ◽  
Rie Tanaka ◽  
Masashi Sumimoto
Keyword(s):  
Biological Treatment ◽  
Color Reversion ◽  
Mechanical Pulps ◽  
Wood Rot Fungi

scholarly journals Safety Evaluation and Whole Genome Sequencing of Aspergillus japonicas PJ01 Reveal Its Potential to Degrade Citrus Segments in Juice Processing

Foods ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1736
Author(s):  
Yujiao Qian ◽  
Zhipeng Gao ◽  
Jieyi Wang ◽  
Chen Wang ◽  
Gaoyang Li ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Gene Annotation ◽  
Gc Content ◽  
Crude Enzyme ◽  
Control Group ◽  
Whole Genome ◽  
Enzyme Solution ◽  
Juice Processing ◽  
Crude Enzyme Solution

Aspergillus japonicas PJ01 (A. japonicas PJ01) is a strain isolated from the rotten branches. In previ-ous studies, it was shown that it can produce complex enzymes to degrade polysaccharide com-ponents. In this study, we evaluated the safety of its crude enzyme solution. Acute oral toxicity, subchronic toxicity, micronucleus and sperm malformation tests all validated the high biologi-cal safety for the crude enzymes. Secondly, we carried out the citrus segment degradation ex-periment of crude enzyme solution. Compared with the control group, the crude enzyme solu-tion of A. japonicas PJ01 can completely degrade the segments in 50 min, which provides the basis for enzymatic peeling during juice processing. The whole genome sequencing showed that the genome of A. japonicus PJ01 has a GC content of 51.37% with a size of 36204647 bp, and encoded 10070 genes. GO, COG, KEGG and CAZy databases were used in gene annotation analyses. Pathway enrichment showed many genes related to carbohydrate metabolism, rich in genes re-lated to pectinase, xylanase and carboxylcellulase. Therefore, the complex enzyme produced by A. japonicus PJ01 can be used in gizzard juice processing to achieve efficient enzymatic decapsu-lation.

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