Fenomen identyfikacji genetycznej

2016 ◽  
Vol 14 (4 (2)) ◽  
pp. 37-49
Author(s):  
Jarosław Moszczyński
Keyword(s):  
Dna Analysis ◽  
Human Identification ◽  
Genetic Identification ◽  
Individual Identification ◽  
Scientific Development ◽  
Disaster Victims ◽  
Short Period ◽  
Golden Standard ◽  
Computer Based ◽  
High Level

DNA identification, which counts a few decades only, has revolutionized forensic science. Over a short period of time, genetic identification has become the most effective method of human identification, even when a tiny amount of biological material is available. Its dynamic development has allowed shifting efforts from group to individual identification. DNA analysis is broadly used for identification of criminal offenders, and due to computer-based DNA data bases – also allows detection of offenders internationally. The technique is fundamental in identification of disaster victims and also historically – in identification of bone remains. Furthermore, it is very useful in verification of wrongful convictions. In civil litigation, DNA analysis is irreplaceable in cases involving paternity testing. Genetic identification has quite solid scientific foundations and therefore, it has dethroned fingerprint identification, being earlier recognized as the “golden” standard of human identification. What is more, DNA analysis triggered the wave of criticism towards the majority of forensic methods of identification, which are considered to be characteristic by a high level of subjectivity, and which started the process of scientific development of these methods.

scholarly journals Genetic DNA Identification from Bone Remains in Kinship Analysis Using Automate Extraction System

2021 ◽  
Author(s):  
Raluca Dumache ◽  
Talida Cut ◽  
Camelia Muresan ◽  
Veronica Ciocan ◽  
Emanuela Stan ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Dna Analysis ◽  
Pcr Amplification ◽  
Human Identification ◽  
Kinship Analysis ◽  
Disaster Victims ◽  
Buccal Swabs ◽  
Bone Powder ◽  
Pcr Products ◽  
Bone Remains

The first ever human identification through DNA analysis was done in the year 1987. Since then, this test has been used, not only in the ruling of civil and juridical cases, but also for human identification of missing persons and mass disaster victims. In this chapter we will present the usefulness of genetic DNA testing of skeletonized remains for human identification, by using automate DNA extraction from three different human bone types: tooth, femur and petrous pyramid. For each case, we obtained saliva samples on buccal swabs from relatives. After the bones were washed and cleaned, Bead Balls Mill Mix 20 (Tehtnica Domel, Slovenia), was used to obtain the bone powder. The DNA extraction from bone samples was performed on the automate Maxwell RSC 48 Instrument (Promega, USA), using the Maxwell FSC DNA IQ Casework Kit (Promega, USA). Power Quant System (Promega, USA) was used for DNA quantification of the samples. The DNA samples were amplified on a Pro Flex PCR System (Thermo Fischer, USA), using the Global Filer PCR Amplification Kit (Applied Biosystems, USA). PCR products were run on a 3500 Genetic Analyzer (Thermo Fischer, USA). Data analysis was performed by Gene Mapper 1.4. Considering that these cases involved DNA extraction from teeth, bones and old human remains, automate system was felt to be the best option to reduce handling errors and increase the possibilities of obtaining good quality DNA.

GENETIC CERTIFICATION OF FODDER CROPS BREEDING ACHIEVEMENTS

1970 ◽  
pp. 40-46
Author(s):  
V. M. Kosolapov ◽  
N. N. Kozlov ◽  
I. А. Klimenko ◽  
V. N. Zolotarev
Keyword(s):  
Dna Analysis ◽  
Molecular Genetic ◽  
Practical Importance ◽  
Genetic Identification ◽  
Necessary Condition ◽  
Forage Crops ◽  
Fodder Crops ◽  
Long Time ◽  
Analytical Review ◽  
Prospects Of Application

The methods of genetic identification of forage crops varieties and forms have significant scientific and practical importance in breeding and seed multiplication, in protection of author’s rights. At the current moment molecular markers on the base of DNA-polymorphism have been applied widely for these aims. This analytical review examines the possibilities and the prospects of application the different DNA-analysis methods for assessment of forage crops genetic diversity and for development the molecular-genetic passports of breeding achievements. The objective estimation of varieties structure and presence impurities is a necessary condition for improving the methodical approaches in approbation of crops and for decision the problems of timely variety-seed renovation and its systematic replacement. The system of DNA markers that registered in genetic passport will enable to keep the initial genetic structure of variety and to maintain it in production process during long time without fluctuations of agronomic important characteristics and properties. This factor is especially valuable for development the primary seed multiplication.

Anxiety, Depression, and Post-traumatic Stress a month after 2019 Cyclone Fani in Odisha, India

2021 ◽  
pp. 1-8
Author(s):  
Nilamadhab Kar ◽  
Narendra Nath Samantaray ◽  
Shreyan Kar ◽  
Brajaballav Kar
Keyword(s):  
Traumatic Stress ◽  
Severe Depression ◽  
Considerable Proportion ◽  
Post Traumatic Stress ◽  
Disaster Victims ◽  
Stress Symptoms ◽  
Short Period ◽  
Post Traumatic ◽  
Anxiety Depression ◽  
Severe Anxiety

Abstract Background: Early Identification of disaster victims with mental health problems may be useful, but information within a short period after a disaster is scarce in developing countries. This study examined anxiety, depression, and post-traumatic stress symptoms at 1 month following 2019 Cyclone Fani in Odisha, India. Method: Post-traumatic stress symptoms (PTSS) were assessed by the Primary care PTSD screen for DSM 5 (PC-PTSD-5), anxiety symptoms by the Generalised Anxiety Disorder (GAD-7), and depression by the Patient Health Questionnaire (PHQ-9). The survey included participants’ disaster experience e.g., evacuation, fear of death, injury, death in family, damage to house, difficulty for food, displacement, and effect on livelihood. Results: Proportion of sample (n = 80) with probable PTSD was 42.9%, with severe anxiety was 36.7%, moderately severe depression was 16.5%, and severe depression was 3.8%. Suicidal cognitions were reported to increase by 14%. Comorbidity was common; with significant (P < 0.01) correlation between PTSS and anxiety (r = 0.69), depression (r = 0.596), and between anxiety and depression (r = 0.63). Damage of house and displacement were associated significantly with PTSD; evacuation and displacement with moderate and severe depression; and displacement with severe anxiety. No specific demographic factors were significantly linked to the psychiatric morbidities. Conclusion: A considerable proportion of victims had psychiatric morbidities at 1 month. Associated risk factors included housing damages, evacuation, and displacement, suggesting the need to improve the disaster-management process.

Geographic Variation in Dominance of Spinosad Resistance in Colorado Potato Beetles (Coleoptera: Chrysomelidae)

2020 ◽  
Author(s):  
Coby Klein ◽  
Mitchell Baker ◽  
Andrei Alyokhin ◽  
David Mota-Sanchez
Keyword(s):  
New York ◽  
Long Island ◽  
New York State ◽  
The United States ◽  
Island Population ◽  
Potato Beetle ◽  
Rate Of Increase ◽  
Short Period ◽  
Leptinotarsa Decemlineata Say ◽  
High Level

Abstract Eastern New York State is frequently the site of Colorado potato beetle (Leptinotarsa decemlineata, Say) populations with the highest observed levels of insecticide resistance to a range of active ingredients. The dominance of a resistant phenotype will affect its rate of increase and the potential for management. On organic farms on Long Island, L. decemlineata evolved high levels of resistance to spinosad in a short period of time and that resistance has spread across the eastern part of the Island. Resistance has also emerged in other parts of the country as well. To clarify the level of dominance or recessiveness of spinosad resistance in different parts of the United States and how resistance differs in separate beetle populations, we sampled in 2010 beetle populations from Maine, Michigan, and Long Island. In addition, a highly resistant Long Island population was assessed in 2012. All populations were hybridized with a laboratory-susceptible strain to determine dominance. None of the populations sampled in 2010 were significantly different from additive resistance, but the Long Island population sampled in 2012 was not significantly different from fully recessive. Recessive inheritance of high-level resistance may help manage its increase.

scholarly journals Analysis of ancient DNA from a prehistoric Amerindian cemetery

1999 ◽  
Vol 354 (1379) ◽  
pp. 153-159 ◽  
Author(s):  
Anne C. Stone ◽  
Mark Stoneking
Keyword(s):  
Ancient Dna ◽  
Dna Analysis ◽  
Nuclear Dna ◽  
Genetic Research ◽  
Hypervariable Region ◽  
Dna Preservation ◽  
European Contact ◽  
Novel Method ◽  
High Level ◽  
Central Illinois

The Norris Farms No. 36 cemetery in central Illinois has been the subject of considerable archaeological and genetic research. Both mitochondrial DNA (mtDNA) and nuclear DNA have been examined in this 700–year–old population. DNA preservation at the site was good, with about 70% of the samples producing mtDNA results and approximately 15% yielding nuclear DNA data. All four of the major Amerindian mtDNA haplogroups were found, in addition to a fifth haplogroup. Sequences of the first hypervariable region of the mtDNA control region revealed a high level of diversity in the Norris Farms population and confirmed that the fifth haplogroup associates with Mongolian sequences and hence is probably authentic. Other than a possible reduction in the number of rare mtDNA lineages in many populations, it does not appear as if European contact significantly altered patterns of Amerindian mtDNA variation, despite the large decrease in population size that occurred. For nuclear DNA analysis, a novel method for DNA–based sex identification that uses nucleotide differences between the X and Y copies of the amelogenin gene was developed and applied successfully in approximately 20 individuals. Despite the well–known problems of poor DNA preservation and the ever–present possibility of contamination with modern DNA, genetic analysis of the Norris Farms No. 36 population demonstrates that ancient DNA can be a fruitful source of new insights into prehistoric populations.

The Legal Form of Financial Institutions as a Way to Protect the Rights of Financial Market Participants

2021 ◽  
Vol 10 (1-2) ◽  
pp. 29-46
Author(s):  
Valentina I. Borisova ◽  
Igor V. Borisov ◽  
Farkhad S. Karagussov
Keyword(s):  
Financial Institutions ◽  
Financial Services ◽  
Scientific Development ◽  
Legal Form ◽  
Market Participants ◽  
Legal Structure ◽  
Legal Forms ◽  
High Level ◽  
Legal Mechanism ◽  
Legal Entities

Abstract Financial institutions are the centre of economic and legal interests of participants of the financial services market, which is itself characterised by a high level of conflict of interests of its participants. The purpose of the article is the scientific development of the legal structure of organisational and legal forms of financial institutions, in the market of financial services, as a legal mechanism for reconciling the economic and legal interests of the main participants of this market. The features of basic and modified legal forms of legal entities are elaborated in this article. It is determined that financial institutions are established and operate in ‘modified’ legal forms. Such forms emerge due to the supplementation of the structure of the main elements of the basic legal forms of legal entities. This refers to additional functional legal means that reflect special requirements for the relevant types of legal entities, depending on the economic and legal interests of their founders/participants.

Comparison of Prescription and Medical Records in Reflecting Patient Antihypertensive Drug Therapy

1994 ◽  
Vol 28 (1) ◽  
pp. 99-104 ◽  
Author(s):  
Dale B. Christensen ◽  
Barbara Williams ◽  
Harold I. Goldberg ◽  
Diane P. Martin ◽  
Ruth Engelberg ◽  
...  
Keyword(s):  
Medical Record ◽  
Medical Records ◽  
Health Maintenance Organizations ◽  
Antihypertensive Drug Therapy ◽  
Antihypertensive Medications ◽  
Pharmacy Records ◽  
Health Maintenance ◽  
Computer Based ◽  
High Level ◽  
Level Of Agreement

OBJECTIVE: To determine the completeness of prescription records, and the extent to which they agreed with medical record drug entries for antihypertensive medications. SETTING: Three clinics affiliated with two staff model health maintenance organizations (HMOs). PARTICIPANTS: Randomly selected HMO enrollees (n=982) with diagnosed hypertension. METHODS: Computer-based prescription records for antihypertensive medications were reviewed at each location using an algorithm to convert the directions-for-use codes into an amount to be consumed per day (prescribed daily dosage). The medical record was analyzed similarly for the presence of drug notations and directions for use. RESULTS: There was a high level of agreement between the medical record and prescription file with respect to identifying the drug prescribed by drug name. Between 5 and 14 percent of medical record drug entries did not have corresponding prescription records, probably reflecting patient decisions not to have prescriptions filled at HMO-affiliated pharmacies or at all. Further, 5–8 percent of dispensed prescription records did not have corresponding medical record drug entry notations, probably reflecting incomplete recording of drug information on the medical record. The percentage of agreement of medical records on dosage ranged from 68 to 70 percent across two sites. Approximately 14 percent of drug records at one location and 21 percent of records at the other had nonmatching dosage information, probably reflecting dosage changes noted on the medical record but not reflected on pharmacy records. CONCLUSIONS: In the sites studied, dispensed prescription records reasonably reflect chart drug entries for drug name, but not necessarily dosage.

scholarly journals Identifying missing people: the contribution of forensic dentistry and DNA

2017 ◽  
Vol 46 (6) ◽  
pp. 313-318
Author(s):  
Luciene Menrique CORRADI ◽  
Denise Vieira TRAVASSOS ◽  
Sylvia Cury COSTE ◽  
Rosa Núbia Vieira de MOURA ◽  
Efigênia Ferreira e FERREIRA
Keyword(s):  
Forensic Anthropology ◽  
Dna Analysis ◽  
Secondary Data ◽  
Human Identification ◽  
Cross Sectional Study ◽  
Medical Institute ◽  
Forensic Dentistry ◽  
Sectional Study ◽  
Cross Sectional ◽  
Dental Examination

Abstract Introduction Human identification is considered one of the major steps concerning missing people. The Forensic Anthropology Sector of Legal Medical Institutes identifies corpses. Forensic dentistry and DNA tests stand out among the existing standard tests. Objective This article aimed to evaluate human identification effectiveness through forensic dental examination performed in the forensic anthropology sector in a Forensic Medical Institute, comparing them with DNA analyses. Methodology This is a cross-sectional study using secondary data available in the department´s database, from 2008 to 2014, concerning identification procedures using forensic dentistry and DNA techniques. Result The analysis of the examinations eligible to this study (241) showed that DNA analysis was the method used for identification in 79.3% of the cases and forensic dental examinations were used in 20.7% of the cases. As for the type of biological material used during these examinations, unidentified corpses corresponded to 131 cases (53.9%), skeleton structures corresponded to 109 cases (44.9%) and there were 3 cases of body segments (1.2%). When analyzing the time spent to complete the tests, dental examinations were faster than DNA tests. The time spent for forensic dental examination does not depend on the type of dental documentation evaluated. Conclusion The analysis of the results in this study showed that human identification through forensic dentistry is effective, rapid and less costly, contributing to greater agility in solving issues related to locating missing people.

scholarly journals Analysis and forecasts of Salmonella spp. antibiotic resistance in Dnipropetrovsk region (Ukraine)

2019 ◽  
pp. 16-19
Author(s):  
H. A. Martynenko
Keyword(s):  
Antibiotic Resistance ◽  
Age Groups ◽  
Quantitative Information ◽  
Graphical Form ◽  
Salmonella Spp ◽  
Gram Negative ◽  
Trend Line ◽  
E Coli ◽  
Short Period ◽  
High Level

The paper presents data of the research aimed at studying the species composition of major pathogens circulating in the region and the resistance to antibacterial drugs in pathogens of salmonella, one of the most common zoonoses. Within the period 2014–2018 the qualitative composition of microflora from biological and pathological materials from different groups of animals was studied in Dnipropetrovsk region. Own research results and the results of the regional veterinary statistical reporting were analyzed. Within the short period of five years, 237 cultures of Gram-negative bacteria were detected and studied. The dominant agent in the studied region was E. coli (56.7%) from the Enterobacteriaceae family. The second most frequent agent in the pathology was Salmonella spp. (10.5%). In total, 29 cultures of salmonella infection were isolated from six species of animals with a predominance of bird isolates. Thus, for different age groups of poultry the most common were S. Gallinarum-Pullorum (56%) and S. Enteritidis (32%). An antibiotic resistance increase in pathogenic salmonella was observed for β-lactam antibiotics (cefazolin, ceftriaxone), aminoglycosides (gentamicin, streptomycin, kanamycin), as well as for tetracycline and polymyxin. Taking into consideration the high level of resistance against norfloxacin in the region’s dominant pathogens of the Enterobacteriaceae family, we performed a forecast in MS Excel graphically and added a trend line. In the course of work it was proved that the Dnipropetrovsk region is a geographic zone with a stable high (86 ± 3.7%) allocation from different groups of animals of Gram-negative microorganisms. It was found that local dominant pathogens are representatives of the Enterobacteriaceae family (E. coli, Salmonella spp.). This data can be used as surrogate resistance markers. The epizootological patterns of animal salmonellosis are determined. It is shown the possibility of forecasting the distribution of antibiotic resistant strains in MS Excel in graphical form by adding a trend line, using quantitative information on the sensitivity of bacteria. Prospects for further research are the prevention and control of the emergence of resistance to antibiotics in veterinary medicine and agriculture in the region and in the country

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