Reentry Capsule Dynamic Instability with Different Aft-body Shape in Transonic Flow

This paper presents a study of transonic wings whose planform shape is curved. Using fluid structure interaction analyses, the dynamic instability conditions were investigated by including the effects of the transonic flow field around oscillating wings. To compare the dynamic aeroelastic characteristics of the curved wing configuration, numerical analyses were carried out on a conventional swept wing and on a curved planform wing. The results confirm that, for a curved planform wing, the dynamic instability condition occurs at higher flight speed if compared to a traditional swept wing with similar profiles, aspect ratio, angle of sweep at root, similar structural layout, and similar mass. A curved wing lifting system could thus improve the performances of future aircrafts.

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Analysis of the Mechanism of Microtubule Dynamic Instability Using a UV Microbeam to Sever Elongating Microtubules

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100102699 ◽

1988 ◽

Vol 46 ◽

pp. 122-123

Author(s):

R.A Walker ◽

S. Inoue ◽

E.D. Salmon

Keyword(s):

Dynamic Instability ◽

Microtubule Dynamic ◽

Gtp Hydrolysis ◽

Abrupt Transition ◽

Microtubule Associated Proteins ◽

Asymmetrical Structure ◽

Associated Proteins

Microtubules polymerized in vitro from tubulin purified free of microtubule-associated proteins exhibit dynamic instability (1,2,3). Free microtubule ends exist in persistent phases of elongation or rapid shortening with infrequent, but, abrupt transitions between these phases. The abrupt transition from elongation to rapid shortening is termed catastrophe and the abrupt transition from rapid shortening to elongation is termed rescue. A microtubule is an asymmetrical structure. The plus end grows faster than the minus end. The frequency of catastrophe of the plus end is somewhat greater than the minus end, while the frequency of rescue of the plus end in much lower than for the minus end (4).The mechanism of catastrophe is controversial, but for both the plus and minus microtubule ends, catastrophe is thought to be dependent on GTP hydrolysis. Microtubule elongation occurs by the association of tubulin-GTP subunits to the growing end. Sometime after incorporation into an elongating microtubule end, the GTP is hydrolyzed to GDP, yielding a core of tubulin-GDP capped by tubulin-GTP (“GTP-cap”).

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Time-Resolved Cryo-Electron Microscopy of Oscillating Microtubules

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100181269 ◽

1990 ◽

Vol 48 (1) ◽

pp. 502-503

Author(s):

Eva-Maria Mandelkow ◽

Ron Milligan

Keyword(s):

Electron Microscopy ◽

Dynamic Instability ◽

Entire Solution ◽

Reaction Scheme ◽

Time Resolved ◽

X Ray ◽

X Ray Scattering ◽

A Chain ◽

Ray Scattering

Microtubules form part of the cytoskeleton of eukaryotic cells. They are hollow libers of about 25 nm diameter made up of 13 protofilaments, each of which consists of a chain of heterodimers of α-and β-tubulin. Microtubules can be assembled in vitro at 37°C in the presence of GTP which is hydrolyzed during the reaction, and they are disassembled at 4°C. In contrast to most other polymers microtubules show the behavior of “dynamic instability”, i.e. they can switch between phases of growth and phases of shrinkage, even at an overall steady state [1]. In certain conditions an entire solution can be synchronized, leading to autonomous oscillations in the degree of assembly which can be observed by X-ray scattering (Fig. 1), light scattering, or electron microscopy [2-5]. In addition such solutions are capable of generating spontaneous spatial patterns [6].In an earlier study we have analyzed the structure of microtubules and their cold-induced disassembly by cryo-EM [7]. One result was that disassembly takes place by loss of protofilament fragments (tubulin oligomers) which fray apart at the microtubule ends. We also looked at microtubule oscillations by time-resolved X-ray scattering and proposed a reaction scheme [4] which involves a cyclic interconversion of tubulin, microtubules, and oligomers (Fig. 2). The present study was undertaken to answer two questions: (a) What is the nature of the oscillations as seen by time-resolved cryo-EM? (b) Do microtubules disassemble by fraying protofilament fragments during oscillations at 37°C?

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