scholarly journals Foreign Gene Expression in the Gonads of Chimaeric Chicken Embryos by Transfer of Primordial Germ Cells Transfected in vitro by Lipofection for 24 Hours

2000 ◽  
Vol 71 (3) ◽  
pp. 308-311
Author(s):  
Mitsuru NAITO ◽  
Yuko MATSUBARA ◽  
Takashi HARUMI ◽  
Takahiro TAGAMI ◽  
Michiharu SAKURAI ◽  
...  
Keyword(s):  
Gene Expression ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Foreign Gene ◽  
Foreign Gene Expression ◽  
Chicken Embryos

scholarly journals Intense Expression of GFP Gene in Gonads of Chicken Embryos by Transfecting Circulating Primordial Germ Cells in vitro and in vivo

2007 ◽  
Vol 44 (4) ◽  
pp. 416-425 ◽  
Author(s):  
Mitsuru Naito ◽  
Takeo Minematsu ◽  
Takashi Harumi ◽  
Takashi Kuwana
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Chicken Embryos ◽  
Gfp Gene

scholarly journals In vivo manipulation of foreign gene expression by steroid administration in the oviduct of laying hens

1999 ◽  
Vol 163 (2) ◽  
pp. 173-179 ◽  
Author(s):  
HM Park ◽  
T Muramatsu
Keyword(s):  
Gene Expression ◽  
Laying Hens ◽  
Foreign Gene ◽  
Foreign Gene Expression ◽  
Response Elements ◽  
Steroid Administration ◽  
Steroid Response ◽  
In Vivo Induction

The experiments described herein were conducted to examine whether or not steroid administration allows in vivo induction of foreign gene expression in the oviduct of laying hens. The chloramphenicol acetyltransferase reporter gene driven by several viral and cellular promoters with or without steroid response elements was transfected by in vivo electroporation. The results indicated that in vivo, as observed in vitro, steroid administration induced transcriptional activities of the promoters with steroid response elements but it did not do so without steroid response elements. Our data implicate, therefore, that in vivo induction of foreign gene expression is possible in the oviduct of laying hens, and that the present in vivo gene transfer approach would serve as a useful tool to elucidate the mechanism of tissue-specific and steroid-induced transcription of chicken egg white genes.

scholarly journals Formative pluripotent stem cells show features of epiblast cells poised for gastrulation

Cell Research ◽  
2021 ◽  
Author(s):  
Xiaoxiao Wang ◽  
Yunlong Xiang ◽  
Yang Yu ◽  
Ran Wang ◽  
Yu Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Germ Cells ◽  
Pluripotent Stem Cells ◽  
Primordial Germ Cells ◽  
Embryonic Stem ◽  
Large Set ◽  
Mouse Escs ◽  
Epiblast Stem Cells ◽  
Early Gastrula

AbstractThe pluripotency of mammalian early and late epiblast could be recapitulated by naïve embryonic stem cells (ESCs) and primed epiblast stem cells (EpiSCs), respectively. However, these two states of pluripotency may not be sufficient to reflect the full complexity and developmental potency of the epiblast during mammalian early development. Here we report the establishment of self-renewing formative pluripotent stem cells (fPSCs) which manifest features of epiblast cells poised for gastrulation. fPSCs can be established from different mouse ESCs, pre-/early-gastrula epiblasts and induced PSCs. Similar to pre-/early-gastrula epiblasts, fPSCs show the transcriptomic features of formative pluripotency, which are distinct from naïve ESCs and primed EpiSCs. fPSCs show the unique epigenetic states of E6.5 epiblast, including the super-bivalency of a large set of developmental genes. Just like epiblast cells immediately before gastrulation, fPSCs can efficiently differentiate into three germ layers and primordial germ cells (PGCs) in vitro. Thus, fPSCs highlight the feasibility of using PSCs to explore the development of mammalian epiblast.

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