scholarly journals In vivo manipulation of foreign gene expression by steroid administration in the oviduct of laying hens

1999 ◽  
Vol 163 (2) ◽  
pp. 173-179 ◽  
Author(s):  
HM Park ◽  
T Muramatsu
Keyword(s):  
Gene Expression ◽  
Laying Hens ◽  
Foreign Gene ◽  
Foreign Gene Expression ◽  
Response Elements ◽  
Steroid Administration ◽  
Steroid Response ◽  
In Vivo Induction

The experiments described herein were conducted to examine whether or not steroid administration allows in vivo induction of foreign gene expression in the oviduct of laying hens. The chloramphenicol acetyltransferase reporter gene driven by several viral and cellular promoters with or without steroid response elements was transfected by in vivo electroporation. The results indicated that in vivo, as observed in vitro, steroid administration induced transcriptional activities of the promoters with steroid response elements but it did not do so without steroid response elements. Our data implicate, therefore, that in vivo induction of foreign gene expression is possible in the oviduct of laying hens, and that the present in vivo gene transfer approach would serve as a useful tool to elucidate the mechanism of tissue-specific and steroid-induced transcription of chicken egg white genes.

scholarly journals Foreign Gene Expression in the Gonads of Chimaeric Chicken Embryos by Transfer of Primordial Germ Cells Transfected in vitro by Lipofection for 24 Hours

2000 ◽  
Vol 71 (3) ◽  
pp. 308-311
Author(s):  
Mitsuru NAITO ◽  
Yuko MATSUBARA ◽  
Takashi HARUMI ◽  
Takahiro TAGAMI ◽  
Michiharu SAKURAI ◽  
...  
Keyword(s):  
Gene Expression ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Foreign Gene ◽  
Foreign Gene Expression ◽  
Chicken Embryos

Foreign gene expression in an organotypic culture of cortical anlage after in vivo electroporation

Neuroreport ◽  
1999 ◽  
Vol 10 (11) ◽  
pp. 2319-2323 ◽  
Author(s):  
Nobuhiko Miyasaka ◽  
Yasuyoshi Arimatsu ◽  
Keiko Takiguchi-Hayashi
Keyword(s):  
Gene Expression ◽  
Organotypic Culture ◽  
Foreign Gene ◽  
In Vivo Electroporation ◽  
Foreign Gene Expression

An integrase facilitates long-lasting foreign gene expression In Vivo in mouse spermatogenic cells

2001 ◽  
Vol 91 (4) ◽  
pp. 363-367 ◽  
Author(s):  
Satoru Ryoki ◽  
Hyi-Man Park ◽  
Yasushige Ohmori ◽  
Akiko Shoji-Tanaka ◽  
Tatsuo Muramatsu
Keyword(s):  
Gene Expression ◽  
Foreign Gene ◽  
Spermatogenic Cells ◽  
Foreign Gene Expression

scholarly journals Comparative analysis of dioxin response elements in human, mouse and rat genomic sequences

2004 ◽  
Vol 32 (15) ◽  
pp. 4512-4523 ◽  
Author(s):  
Y. V. Sun ◽  
D. R. Boverhof ◽  
L. D. Burgoon ◽  
M. R. Fielden ◽  
T. R. Zacharewski
Keyword(s):  
Gene Expression ◽  
Transcriptional Start Site ◽  
Genomic Sequences ◽  
Mouse Tissue ◽  
Start Site ◽  
Orthologous Genes ◽  
Multiple Sequence ◽  
Response Elements

Abstract Comparative approaches were used to identify human, mouse and rat dioxin response elements (DREs) in genomic sequences unambiguously assigned to a nucleotide RefSeq accession number. A total of 13 bona fide DREs, all including the substitution intolerant core sequence (GCGTG) and adjacent variable sequences, were used to establish a position weight matrix and a matrix similarity (MS) score threshold to rank identified DREs. DREs with MS scores above the threshold were disproportionately distributed in close proximity to the transcription start site in all three species. Gene expression assays in hepatic mouse tissue confirmed the responsiveness of 192 genes possessing a putative DRE. Previously identified functional DREs in well-characterized AhR-regulated genes including Cyp1a1 and Cyp1b1 were corroborated. Putative DREs were identified in 48 out of 2437 human–mouse–rat orthologous genes between −1500 and the transcriptional start site, of which 19 of these genes possessed positionally conserved DREs as determined by multiple sequence alignment. Seven of these nineteen genes exhibited 2,3,7,8-tetrachlorodibenzo- p -dioxin-mediated regulation, although there were significant discrepancies between in vivo and in vitro results. Interestingly, of the mouse–rat orthologous genes with a DRE between −1500 and +1500, only 37% had an equivalent human ortholog. These results suggest that AhR-mediated gene expression may not be well conserved across species, which could have significant implications in human risk assessment.

Phenotypic and immunologic factors affecting plasmid-mediated in vivo gene transfer to rat diaphragm

1996 ◽  
Vol 270 (6) ◽  
pp. L1023-L1030 ◽  
Author(s):  
B. J. Petrof ◽  
G. Acsadi ◽  
J. Bourdon ◽  
N. Matusiewicz ◽  
L. Yang
Keyword(s):  
Gene Expression ◽  
Gene Transfer ◽  
Reporter Gene ◽  
Transfection Efficiency ◽  
Foreign Gene ◽  
Rat Diaphragm ◽  
Foreign Gene Expression ◽  
Hindlimb Muscles ◽  
In Vivo Gene Transfer

Little is known about the molecular mechanisms governing adaptive responses of the diaphragm in the setting of lung disease. By permitting the study of regulatory elements and the effects of overexpressing genes of interest, direct in vivo gene transfer to the diaphragm could be used as a tool to address such questions. Therefore, we evaluated parameters affecting transfection efficiency and duration of foreign gene expression in the diaphragm after plasmid-mediated gene transfer. Reporter gene constructs were injected into adult rat diaphragm and hindlimb muscles. Transfection efficiency at 8-10 days postinjection was decreased in large caliber ( > 1,000 microns2) and type II myosin heavy chain (MHC)-expressing fibers. There were also strong trends toward augmented transfection efficiency in type I MHC- and embryonic MHC-expressing fibers. All diaphragms demonstrated evidence of muscle injury and inflammatory cell infiltrates at this early time point. By 30 days postinjection, however, neither inflammation nor reporter gene expression was detectable in diaphragm or hindlimb muscles of immunocompetent animals. By contrast, immunosuppressed rats (given cyclosporine; 15 mg.kg-1. day-1) showed high levels of foreign gene expression at 30 days postinjection, which remained stable up to 60 days. Therefore, exploitation of plasmid-mediated in vivo gene transfer as a tool for studying regulated gene expression in the diaphragm may be facilitated by the use of immunodeficient animal models.

Ultrasound/Microbubble Enhances Foreign Gene Expression in ECV304 Cells and Murine Myocardium

2004 ◽  
Vol 36 (12) ◽  
pp. 824-831 ◽  
Author(s):  
Dongping Guo ◽  
Xiaoyu Li ◽  
Ping Sun ◽  
Zhiguang Wang ◽  
Xiuying Chen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Therapy ◽  
Plasmid Dna ◽  
Transfection Efficiency ◽  
Ultrasound Irradiation ◽  
Ultrasound Contrast Agents ◽  
Foreign Gene ◽  
Exogenous Gene

Abstract Although viral vectors are efficient systems to transfer foreign genes into cells or target tissues, safety issues remain in relation to human gene therapy. Microbubbles currently used as ultrasound contrast agents have been applied in transfection of genes. This study was designed to test the transfection efficiency and the expression of exogenous gene mediated by ultrasound irradiation enhanced air filled albumin microbubbles in ECV304 cell line in vitro and the heart of the mouse in vivo. Air filled microbubbles (2.0–4.0 μm in diameter) were created by sonicating the mixture of human albumin, glucose, mannitol and special additive that was designed for stabilization. Plasmid DNA loading the reporter genes was gently mixed with microbubbles. The mixture of plasmid DNA and microbubbles was administrated to cultured ECV304 cells and BALB/c mice (tail vein injection) under different ultrasound/microbubble conditions, and then the transfection and expression efficiency were examined. The results both in vivo and in vitro demonstrated that microbubble with ultrasound irradiation could significantly elevate the exogenous gene expression as compared with microbubble or ultrasound only. Overall, the present study showed that the ultrasound-target microbubble destruction method enhanced the exogenous gene expression in vivo and in vitro, and provided a gene therapy way not only efficient but also easy to be manipulated and carried out in clinical.

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