scholarly journals An Evalution of Measurement of Cell Growth in Tissue Culture with Protein Quantitation

1992 ◽  
Vol 46 (2) ◽  
pp. 398-403
Author(s):  
Sen Higashi ◽  
Tomoko Ohsumi ◽  
Kayoko Kuroki
Keyword(s):  
Tissue Culture ◽  
Cell Growth ◽  
Protein Quantitation

Hydrophilic 1-(carboxymethyl)-5-fluorouracil amides: Preparation and cytostatic activity

1987 ◽  
Vol 52 (8) ◽  
pp. 2061-2069 ◽  
Author(s):  
Helmut Pischel ◽  
Antonín Holý ◽  
Jiří Veselý ◽  
Günther Wagner ◽  
Dieter Cech
Keyword(s):  
Tissue Culture ◽  
Cell Growth ◽  
Cytostatic Activity ◽  
Nitrophenyl Ester ◽  
Murine Leukemia

The following N-substituted amides IV were prepared by reaction of 1-(carboxymethyl)-5-fluorouracil p-nitrophenyl ester (II) with primary or secondary hydroxyalkylamines III: 2-hydroxypropyl (IVa), 3-hydroxypropyl (IVb), 2,3-dihydroxypropyl (IVc), 3-hydroxy-2-methyl-2-propyl (IVd), 1,3-dihydroxy-2-propyl (IVe), 1-deoxyglucitol-1-yl (IVg), 2-deoxyglucitol-2-yl (IVh), methyl-2,3-dihydroxypropyl (IVi), methyl-1-deoxyglucitol-1-yl (IVk), and n-butyl-2,3-dihydroxypropyl (IVl). None of these compounds had any cytostatic activity towards murine leukemia L-1210 cell growth in a tissue culture at 10-5 mol l-1.

Unique method of tissue culture preparation for EM

1992 ◽  
Vol 50 (1) ◽  
pp. 728-729
Author(s):  
Fen Wang ◽  
Lindsay B. Ledford ◽  
Jonathan F. Head ◽  
Robert L. Elliott
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Cell Growth ◽  
Crystal Violet ◽  
Microscopic Examination ◽  
Culture Medium ◽  
Electron Microscopic Examination ◽  
Electron Microscopic ◽  
Unique Method ◽  
Mcf 7

A simplified technique of growing monolayer cells for electron microscopic examination has been developed. Our procedure has eliminated many difficult steps and therefore is easier than those reported by others.Regular Beem capsules for routine embedding for electron microscopy were used(Fig. 1). Prior to tissue inoculation capsules were washed with 5% HCl and gas sterilized. A 0.5 ml cell suspension of MCF-7 cells (10,000/ml) was placed in the capsules and cells settled in the pyramid portion (Fig. 2). Culture medium was alpha-MEM with 10% FCS. Capsules were incubated at 37°C for 3 days. They were then fixed in 70% ethanol for 20 minutes and stained with crystal violet. The pyramid portion of the capsule was cut off and monolayer cell growth was confirmed by examination under a microscope (Fig. 3).

Inquiry into the Limits of Biological Effects of Chemical Compounds in Tissue Culture, I

1975 ◽  
Vol 30 (9-10) ◽  
pp. 643-649 ◽  
Author(s):  
Jan Diek van Mansvelt ◽  
Ferdinand Amons
Keyword(s):  
Tissue Culture ◽  
Cell Growth ◽  
Mercuric Chloride ◽  
Low Dose ◽  
Biological Effects ◽  
Chemical Compounds ◽  
Dose Effect ◽  
The Third ◽  
Effect Relation ◽  
Growth Inhibiting

Experiments were designed to investigate the low-dose side of the so-called dose-effect relation of mercuric chloride on the proliferation of a murine lymphoblastic cell strain (MB VIa). Three concentration ranges, from 0.9 × 10-5 м to 0.9 × 10-18 м, from 0.9 × 10-12 м to 0.9 × 10-25 м , and from 0.9 × 10-15 м to 0.9 x 10-21 м, in which the concentration decreased stepwise by a factor 10, were studied in 61, 74 and 58 experimental repetitions, respectively. In the first range, the concentrations 0.9 × 10-5 M and 0.9 x 10-6 м HgCl2 proved to be definitely toxic as was expected. However, also 0.9 × 10-16 м and 0.9 × 10-17 м appeared to be growth-inhibiting, the latter results being confirmed in the second and the third concentration ranges. These differences in cell growth were statistically significant.

scholarly journals Adaptation of plastic surfaces for tissue culture by glow discharge

1975 ◽  
Vol 2 (1) ◽  
pp. 46-54
Author(s):  
C F Amstein ◽  
P A Hartman
Keyword(s):  
Tissue Culture ◽  
Cell Growth ◽  
Glow Discharge ◽  
Lung Cells ◽  
Discharge Unit ◽  
Plastic Surfaces ◽  
Electrically Charged ◽  
Open Growth ◽  
Chicken Embryo Fibroblasts ◽  
Canine Kidney

Plastic petri dishes and microtitration plates were electrically charged by a glow discharge unit installed in a vacuum evaporator. Charged and uncharged plates, as well as plates commercially treated for tissue culture, were inoculated with Vero and BHK-21 cell lines; secondary cultures of monkey kidney, chicken lung, canine kidney, and embryonic bovine kidney; and primary chicken embryo fibroblasts and chicken lung cells. All cell cultures grew normally on petri plates charged with the covers open. Growth rate and cell density compared favorably with growth on the commercial tissue culture plates; cell growth was somewhat less dense, however, on plates charged with the covers closed. Charged plates could be sterilized by ultraviolet light and ethylene oxide with no adverse effects on cell growth. Cells inoculated onto plates charged up to 7 months before inoculation grew as well as on freshly charged plates.

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