Hydrophilic 1-(carboxymethyl)-5-fluorouracil amides: Preparation and cytostatic activity

1987 ◽  
Vol 52 (8) ◽  
pp. 2061-2069 ◽  
Author(s):  
Helmut Pischel ◽  
Antonín Holý ◽  
Jiří Veselý ◽  
Günther Wagner ◽  
Dieter Cech
Keyword(s):  
Tissue Culture ◽  
Cell Growth ◽  
Cytostatic Activity ◽  
Nitrophenyl Ester ◽  
Murine Leukemia

The following N-substituted amides IV were prepared by reaction of 1-(carboxymethyl)-5-fluorouracil p-nitrophenyl ester (II) with primary or secondary hydroxyalkylamines III: 2-hydroxypropyl (IVa), 3-hydroxypropyl (IVb), 2,3-dihydroxypropyl (IVc), 3-hydroxy-2-methyl-2-propyl (IVd), 1,3-dihydroxy-2-propyl (IVe), 1-deoxyglucitol-1-yl (IVg), 2-deoxyglucitol-2-yl (IVh), methyl-2,3-dihydroxypropyl (IVi), methyl-1-deoxyglucitol-1-yl (IVk), and n-butyl-2,3-dihydroxypropyl (IVl). None of these compounds had any cytostatic activity towards murine leukemia L-1210 cell growth in a tissue culture at 10-5 mol l-1.

Recent Studies on a Murine Leukemia Virus Grown in Tissue Culture

1967 ◽  
Vol 25 ◽  
pp. 106-107
Author(s):  
L. Z. de Tkaczevski ◽  
E. de Harven ◽  
C. Friend
Keyword(s):  
Tissue Culture ◽  
Solid Tumors ◽  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Supernatant Fluid ◽  
Virus Particles ◽  
Friend Leukemia ◽  
Type C ◽  
Leukemia Viruses ◽  
Murine Leukemia

Despite extensive studies, the correlation between the morphology and pathogenicity of murine leukemia viruses (MLV) has not yet been clarified. The virus particles found in the plasma of leukemic mice belong to 2 distinct groups, 1 or 2% of them being enveloped A particles and the vast majority being of type C. It is generally believed that these 2 types of particles represent different phases in the development of the same virus. Particles of type A have been thought to be an earlier form of type C particles. One of the tissue culture lines established from Friend leukemia solid tumors has provided the material for the present study. The supernatant fluid of the line designated C-1A contains an almost pure population of A particles as illustrated in Figure 1. The ratio is, therefore, the reverse of what is unvariably observed in the plasma of leukemic mice where C particles predominate.

scholarly journals An Evalution of Measurement of Cell Growth in Tissue Culture with Protein Quantitation

1992 ◽  
Vol 46 (2) ◽  
pp. 398-403
Author(s):  
Sen Higashi ◽  
Tomoko Ohsumi ◽  
Kayoko Kuroki
Keyword(s):  
Tissue Culture ◽  
Cell Growth ◽  
Protein Quantitation

Unique method of tissue culture preparation for EM

1992 ◽  
Vol 50 (1) ◽  
pp. 728-729
Author(s):  
Fen Wang ◽  
Lindsay B. Ledford ◽  
Jonathan F. Head ◽  
Robert L. Elliott
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Cell Growth ◽  
Crystal Violet ◽  
Microscopic Examination ◽  
Culture Medium ◽  
Electron Microscopic Examination ◽  
Electron Microscopic ◽  
Unique Method ◽  
Mcf 7

A simplified technique of growing monolayer cells for electron microscopic examination has been developed. Our procedure has eliminated many difficult steps and therefore is easier than those reported by others.Regular Beem capsules for routine embedding for electron microscopy were used(Fig. 1). Prior to tissue inoculation capsules were washed with 5% HCl and gas sterilized. A 0.5 ml cell suspension of MCF-7 cells (10,000/ml) was placed in the capsules and cells settled in the pyramid portion (Fig. 2). Culture medium was alpha-MEM with 10% FCS. Capsules were incubated at 37°C for 3 days. They were then fixed in 70% ethanol for 20 minutes and stained with crystal violet. The pyramid portion of the capsule was cut off and monolayer cell growth was confirmed by examination under a microscope (Fig. 3).

Adaptation to Tissue Culture of the Murine Leukemia ASL1, a High Producer of TL (Thymus Leukemia) Antigen1

2008 ◽  
Vol 6 (4) ◽  
pp. 335-341 ◽  
Author(s):  
Jackie L. Williams ◽  
Thomas H. Stanton ◽  
R. Michael Wolcott
Keyword(s):  
Tissue Culture ◽  
Murine Leukemia

scholarly journals THE USE OF MILLIPORE FILTERS IN ULTRASTRUCTURAL STUDIES OF CELL CULTURES AND VIRUSES

1968 ◽  
Vol 36 (1) ◽  
pp. 231-243 ◽  
Author(s):  
Robert M. McCombs ◽  
Matilda Benyesh-Melnick ◽  
J. Pierre Brunschwig
Keyword(s):  
Tissue Culture ◽  
Plasma Membranes ◽  
Fibroblast Cells ◽  
Virus Particles ◽  
Ultrastructural Studies ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Satellite Viruses ◽  
Murine Leukemia ◽  
Millipore Filters

A technique is described for embedding tissue culture cells that have been adsorbed or grown on Millipore filters. The acetone used during the embedding process rendered the filters transparent so that specific areas or cells could be chosen with the aid of the light microscope. Lymphoblastoid cells processed on the filters possessed well-defined plasma membranes and microvilli which were rarely present in cells from parallel cultures that were prepared by pelleting in the centrifuge. Fibroblast cells grown on filters retained their elongated appearance, in contrast to the rounded cells in pelleted preparations. Millipore filters were also used as a means of embedding virus pellets for sectioning. Preparations containing as few as 4 x 108 virus particles were suitable for study by the filter technique. Crude tissue-culture harvests of vaccinia virus and purified preparations of Rauscher murine leukemia and adeno-satellite viruses were successfully examined.

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