Comparison of two-component signal transduction systems in five bacterial stains related with the adsorption of rare earth elements

2014 ◽  
Author(s):  
S.J. Yu ◽  
T. Peng ◽  
Y.P. Peng ◽  
D. Zhou
Keyword(s):  
Signal Transduction ◽  
Rare Earth ◽  
Rare Earth Elements ◽  
Two Component ◽  
Two Component Signal Transduction ◽  
Signal Transduction Systems

scholarly journals Rewiring the Specificity of Two-Component Signal Transduction Systems

Cell ◽  
2008 ◽  
Vol 133 (6) ◽  
pp. 1043-1054 ◽  
Author(s):  
Jeffrey M. Skerker ◽  
Barrett S. Perchuk ◽  
Albert Siryaporn ◽  
Emma A. Lubin ◽  
Orr Ashenberg ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Two Component ◽  
Two Component Signal Transduction ◽  
Signal Transduction Systems

Kinetic and mechanistic analyses of new classes of inhibitors of two-component signal transduction systems using a coupled assay containing HpkA–DrrA from Thermotoga maritima

Microbiology ◽  
2004 ◽  
Vol 150 (4) ◽  
pp. 885-896 ◽  
Author(s):  
J. Estelle Foster ◽  
Qin Sheng ◽  
Jonathan R. McClain ◽  
Mark Bures ◽  
Thalia I. Nicas ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Histidine Kinase ◽  
Competitive Inhibition ◽  
Thermotoga Maritima ◽  
Phosphorylation Site ◽  
Phosphoryl Group ◽  
Two Component ◽  
Two Component Signal Transduction ◽  
Coupled Assay ◽  
Signal Transduction Systems

Two-component signal transduction systems (TCSs) play fundamental roles in bacterial survival and pathogenesis and have been proposed as targets for the development of novel classes of antibiotics. A new coupled assay was developed and applied to analyse the kinetic mechanisms of three new kinds of inhibitors of TCS function. The assay exploits the biochemical properties of the cognate HpkA–DrrA histidine kinase–response regulator pair from Thermotoga maritima and allows multiple turnovers of HpkA, linear formation of phosphorylated DrrA, and Michaelis–Menten analysis of inhibitors. The assay was validated in several ways, including confirmation of competitive inhibition by adenosine 5′-β,γ-imidotriphosphate (AMP-PNP). The coupled assay, autophosphorylation and chemical cross-linking were used to determine the mechanisms by which several compounds inhibit TCS function. A cyanoacetoacetamide showed non-competitive inhibition with respect to ATP concentration in the coupled assay. The cyanoacetoacetamide also inhibited autophosphorylation of histidine kinases from other bacteria, indicating that the coupled assay could detect general inhibitors of histidine kinase function. Inhibition of HpkA autophosphorylation by this compound was probably caused by aggregation of HpkA, consistent with a previous model for other hydrophobic compounds. In contrast, ethodin was a potent inhibitor of the combined assay, did not inhibit HpkA autophosphorylation, but still led to aggregation of HpkA. These data suggest that ethodin bound to the HpkA kinase and inhibited transfer of the phosphoryl group to DrrA. A peptide corresponding to the phosphorylation site of DrrA appeared to inhibit TCS function by a mechanism similar to that of ethodin, except that autophosphorylation was inhibited at high peptide concentrations. The latter mechanism of inhibition of TCS function is unusual and its analysis demonstrates the utility of these approaches to the kinetic analyses of additional new classes of inhibitors of TCS function.

scholarly journals Two-Component Signal Transduction Systems in the Human Pathogen Streptococcus agalactiae

2020 ◽  
Vol 88 (7) ◽  
Author(s):  
Lamar Thomas ◽  
Laura Cook
Keyword(s):  
Signal Transduction ◽  
Streptococcus Agalactiae ◽  
Metabolic Regulation ◽  
Invasive Infection ◽  
Older Individuals ◽  
Content Type ◽  
Two Component ◽  
Two Component Signal Transduction ◽  
Group B ◽  
Signal Transduction Systems

ABSTRACT Streptococcus agalactiae (group B Streptococcus [GBS]) is an important cause of invasive infection in newborns, maternal women, and older individuals with underlying chronic illnesses. GBS has many mechanisms to adapt and survive in its host, and these mechanisms are often controlled via two-component signal transduction systems. In GBS, more than 20 distinct two-component systems (TCSs) have been classified to date, consisting of canonical TCSs as well as orphan and atypical sensors and regulators. These signal transducing systems are necessary for metabolic regulation, resistance to antibiotics and antimicrobials, pathogenesis, and adhesion to the mucosal surfaces to colonize the host. This minireview discusses the structures of these TCSs in GBS as well as how selected systems regulate essential cellular processes such as survival and colonization. GBS contains almost double the number of TCSs compared to the closely related Streptococcus pyogenes and Streptococcus pneumoniae, and while research on GBS TCSs has been increasing in recent years, no comprehensive reviews of these TCSs exist, making this review especially relevant.

Sign in / Sign up

Export Citation Format

Share Document