scholarly journals Dental pulp responses to biphasic calcium phosphate cement containing buffer solution in the liquid phase

2005 ◽  
Author(s):  
M. Yoshikawa ◽  
T. Toda
Keyword(s):  
Liquid Phase ◽  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Dental Pulp ◽  
Biphasic Calcium Phosphate ◽  
Buffer Solution

The pH-Dependent Properties of the Biphasic Calcium Phosphate for Bone Cements

2014 ◽  
Vol 21 ◽  
pp. 3-16 ◽  
Author(s):  
Nuan La Ong Srakaew ◽  
Sirirat Tubsungnoen Rattanachan
Keyword(s):  
Compressive Strength ◽  
Liquid Phase ◽  
Calcium Phosphate ◽  
Phase Analysis ◽  
Calcium Phosphate Cement ◽  
Biphasic Calcium Phosphate ◽  
Setting Time ◽  
Bone Cements ◽  
Apatite Formation ◽  
Apatite Cement

Self-setting calcium phosphate cement (CPC) has been used in bone repair and substitution due to their excellent biocompatibility, bioactive as well as simplicity of preparation and use. The inherent brittleness and slow degradation are the major disadvantages for the use of calcium phosphate cements. To improve the degradation for the traditional CPC, the apatite cement formula incorporated with β-tricalcium phosphate (β-TCP) with varying concentration were studied and the effect of the pH value of liquid phase on the properties of this new calcium phosphate cement formula was evaluated. The apatite cements containing β-TCP for 10 and 40 wt.% were mixed into the aqueous solution with different pH values and then aging in absolute humidity at 37°C for 7 days. The setting time and phase analysis of the biphasic calcium phosphate were determined as compared to the apatite cement. For proper medical application, the compressive strength, the phase analysis and the degradation of the CPC samples at pH 7.0 and 7.4 were evaluated after soaking in the simulated body fluid (SBF) at 37°C for 7 days. The results indicated that the properties of the samples such as the setting time, the compressive strength related to the phase analysis of the set cements. The high degradation of the CPC was found in the cement with increasing β-TCP addition due to the phase after setting. Apatite formation with oriented plate-like morphology was also found to be denser on the surface of the biphasic bone cements after soaking in SBF for 7 days. The obtained results indicated that the cement containing β-TCP mixed with the liquid phase at pH 7.4 could be considered as a highly biodegradable and bioactive bone cement, as compared to the traditional CPC.

scholarly journals A New Type of Biphasic Calcium Phosphate Cement as a Gentamicin Carrier for Osteomyelitis

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Wen-Yu Su ◽  
Yu-Chun Chen ◽  
Feng-Huei Lin
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Biphasic Calcium Phosphate ◽  
Setting Time ◽  
Phosphate Buffer Solution ◽  
Infrared Spectrometry ◽  
Final Phase ◽  
Buffer Solution ◽  
Alternative Treatment Option ◽  
High Doses

Osteomyelitis therapy is a long-term and inconvenient procedure for a patient. Antibiotic-loaded bone cements are both a complementary and alternative treatment option to intravenous antibiotic therapy for the treatment of osteomyelitis. In the current study, the biphasic calcium phosphate cement (CPC), calledα-TCP/HAP (α-tricalcium phosphate/hydroxyapatite) biphasic cement, was prepared as an antibiotics carrier for osteomyelitis. The developed biphasic cement with a microstructure ofα-TCP surrounding the HAP has a fast setting time which will fulfill the clinical demand. The X-ray diffraction and Fourier transform infrared spectrometry analyses showed the final phase to be HAP, the basic bone mineral, after setting for a period of time. Scanning electron microscopy revealed a porous structure with particle sizes of a few micrometers. The addition of gentamicin inα-TCP/HAP would delay the transition ofα-TCP but would not change the final-phase HAP. The gentamicin-loadedα-TCP/HAP supplies high doses of the antibiotic during the initial 24 hours when they are soaked in phosphate buffer solution (PBS). Thereafter, a slower drug release is produced, supplying minimum inhibitory concentration until the end of the experiment (30 days). Studies of growth inhibition ofStaphylococcus aureusandPseudomonas aeruginosain culture indicated that gentamicin released after 30 days fromα-TCP/HAP biphasic cement retained antibacterial activity.

scholarly journals Enhanced Osteogenesis of Dental Pulp Stem Cells In Vitro Induced by Chitosan–PEG-Incorporated Calcium Phosphate Cement

Polymers ◽  
2021 ◽  
Vol 13 (14) ◽  
pp. 2252
Author(s):  
Jae Eun Kim ◽  
Sangbae Park ◽  
Woong-Sup Lee ◽  
Jinsub Han ◽  
Jae Woon Lim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Calcium Phosphate ◽  
Zeta Potential ◽  
Mechanical Strength ◽  
Calcium Phosphate Cement ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Ion Trapping ◽  
Alizarin Red

The use of bone graft materials is required for the treatment of bone defects damaged beyond the critical defect; therefore, injectable calcium phosphate cement (CPC) is actively used after surgery. The application of various polymers to improve injectability, mechanical strength, and biological function of injection-type CPC is encouraged. We previously developed a chitosan–PEG conjugate (CS/PEG) by a sulfur (VI) fluoride exchange reaction, and the resulting chitosan derivative showed high solubility at a neutral pH. We have demonstrated the CPC incorporated with a poly (ethylene glycol) (PEG)-grafted chitosan (CS/PEG) and developed CS/PEG CPC. The characterization of CS/PEG CPC was conducted using Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD). The initial properties of CS/PEG CPCs, such as the pH, porosity, mechanical strength, zeta potential, and in vitro biocompatibility using the WST-1 assay, were also investigated. Moreover, osteocompatibility of CS/PEG CPCs was carried out via Alizarin Red S staining, immunocytochemistry, and Western blot analysis. CS/PEG CPC has enhanced mechanical strength compared to CPC, and the cohesion test also demonstrated in vivo stability. Furthermore, we determined whether CS/PEG CPC is a suitable candidate for promoting the osteogenic ability of Dental Pulp Stem Cells (DPSC). The elution of CS/PEG CPC entraps more calcium ion than CPC, as confirmed through the zeta potential test. Accordingly, the ion trapping effect of CS/PEG is considered to have played a role in promoting osteogenic differentiation of DPSCs. The results strongly suggested that CS/PEG could be used as suitable additives for improving osteogenic induction of bone substitute materials.

Genotoxicity assessment of biphasic calcium phosphate of modified porosity on human dental pulp cells using Ames and Comet assays

2018 ◽  
Vol 47 ◽  
pp. 207-212 ◽  
Author(s):  
Nur Fathin Alia Che Wahab ◽  
Thirumulu Ponnuraj Kannan ◽  
Zuliani Mahmood ◽  
Ismail Ab. Rahman ◽  
Hanafi Ismail
Keyword(s):  
Calcium Phosphate ◽  
Dental Pulp ◽  
Biphasic Calcium Phosphate ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Human Dental Pulp ◽  
Pulp Cells

Effect of Protein Addition on the Setting Behaviour of a Calcium Phosphate Cement

2006 ◽  
Vol 309-311 ◽  
pp. 841-844
Author(s):  
S. Chauhan ◽  
M.P. Hofmann ◽  
R.M. Shelton
Keyword(s):  
Compressive Strength ◽  
Liquid Phase ◽  
Calcium Phosphate ◽  
Mechanical Strength ◽  
Calcium Phosphate Cement ◽  
Tricalcium Phosphate ◽  
Bovine Serum ◽  
Setting Time ◽  
Monocalcium Phosphate ◽  
Foetal Bovine Serum

This study investigated the influence of the addition of various proteins to the liquid phase (albumin, fibrinogen and foetal bovine serum (FBS)) on the mechanical strength and setting time of a brushite forming calcium phosphate cement. Additions of 1wt% protein to the liquid phase led to a deterioration in compressive strength of the set cement by up to 50%. The setting time was not affected by adding albumin and FBS but was increased by 50% with admixtures containing fibrinogen. The conversion of the reactants, β-tricalcium phosphate and monocalcium phosphate, to brushite was found to be unaffected by addition of up to 10wt% proteins.

scholarly journals Novel Calcium Phosphate Cement with Metformin-Loaded Chitosan for Odontogenic Differentiation of Human Dental Pulp Cells

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Wei Qin ◽  
Jia-Yao Chen ◽  
Jia Guo ◽  
Tao Ma ◽  
Michael D. Weir ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Odontogenic Differentiation ◽  
Human Dental Pulp ◽  
Pulp Cells ◽  
Chitosan Composite ◽  
Dentin Regeneration

Metformin is an old and widely accepted first-line drug for treating type 2 diabetes. Our previous studies demonstrate that metformin can stimulate the osteo/odontogenic differentiation of human-induced pluripotent stem cell-derived mesenchymal stem cells and human dental pulp cells (DPCs). Due to the rapid dilution of metformin from the defect area, the aim of this study was to develop a drug delivery system with controlled release of metformin to promote cell viability and odontogenic differentiation of DPCs favoring dentin regeneration. Calcium phosphate cement (CPC) containing chitosan and metformin as a scaffold was synthesized. DPCs were seeded onto the scaffold, and the viability and proliferation were evaluated at several time points. For osteogenic differentiation analysis, alkaline phosphatase (ALP) activity was tested, cells were stained with Alizarin Red, and the expression of odontogenic markers was evaluated by real-time polymerase chain reaction. DPCs remained viable and attached well to the CPC-chitosan composite scaffold. Moreover, the addition of metformin to the CPC-chitosan composite did not adversely affect cell proliferation, compared to that of CPC control. Our data further revealed that the novel CPC-chitosan-metformin composite enhanced the odontogenic differentiation of DPCs, as evidenced by higher ALP activity, elevated expression of odontoblastic markers, and strong mineral deposition. These results suggest that the new CPC-chitosan-metformin composite is a highly promising scaffold with the potential for tissue engineering applications including dentin regeneration.

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