Osteogenic stimulation of human dental pulp stem cells with self‐setting biphasic calcium phosphate cement

2020 ◽  
Vol 108 (4) ◽  
pp. 1669-1678
Author(s):  
Yingzhi Gu ◽  
Rui Zhuang ◽  
Xianju Xie ◽  
Yuxing Bai
Keyword(s):  
Stem Cells ◽  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Dental Pulp ◽  
Biphasic Calcium Phosphate ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp ◽  
Stimulation Of

Porous clinoptilolite—nano biphasic calcium phosphate scaffolds loaded with human dental pulp stem cells for load bearing orthopedic applications

2019 ◽  
Vol 14 (5) ◽  
pp. 055010 ◽  
Author(s):  
Ammar Z Alshemary ◽  
Ahmet Engin Pazarçeviren ◽  
Dilek Keskin ◽  
Aysen Tezcaner ◽  
Rafaqat Hussain ◽  
...  
Keyword(s):  
Stem Cells ◽  
Calcium Phosphate ◽  
Dental Pulp ◽  
Biphasic Calcium Phosphate ◽  
Dental Pulp Stem Cells ◽  
Load Bearing ◽  
Human Dental Pulp ◽  
Orthopedic Applications

scholarly journals Enhanced Osteogenesis of Dental Pulp Stem Cells In Vitro Induced by Chitosan–PEG-Incorporated Calcium Phosphate Cement

Polymers ◽  
2021 ◽  
Vol 13 (14) ◽  
pp. 2252
Author(s):  
Jae Eun Kim ◽  
Sangbae Park ◽  
Woong-Sup Lee ◽  
Jinsub Han ◽  
Jae Woon Lim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Calcium Phosphate ◽  
Zeta Potential ◽  
Mechanical Strength ◽  
Calcium Phosphate Cement ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Ion Trapping ◽  
Alizarin Red

The use of bone graft materials is required for the treatment of bone defects damaged beyond the critical defect; therefore, injectable calcium phosphate cement (CPC) is actively used after surgery. The application of various polymers to improve injectability, mechanical strength, and biological function of injection-type CPC is encouraged. We previously developed a chitosan–PEG conjugate (CS/PEG) by a sulfur (VI) fluoride exchange reaction, and the resulting chitosan derivative showed high solubility at a neutral pH. We have demonstrated the CPC incorporated with a poly (ethylene glycol) (PEG)-grafted chitosan (CS/PEG) and developed CS/PEG CPC. The characterization of CS/PEG CPC was conducted using Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD). The initial properties of CS/PEG CPCs, such as the pH, porosity, mechanical strength, zeta potential, and in vitro biocompatibility using the WST-1 assay, were also investigated. Moreover, osteocompatibility of CS/PEG CPCs was carried out via Alizarin Red S staining, immunocytochemistry, and Western blot analysis. CS/PEG CPC has enhanced mechanical strength compared to CPC, and the cohesion test also demonstrated in vivo stability. Furthermore, we determined whether CS/PEG CPC is a suitable candidate for promoting the osteogenic ability of Dental Pulp Stem Cells (DPSC). The elution of CS/PEG CPC entraps more calcium ion than CPC, as confirmed through the zeta potential test. Accordingly, the ion trapping effect of CS/PEG is considered to have played a role in promoting osteogenic differentiation of DPSCs. The results strongly suggested that CS/PEG could be used as suitable additives for improving osteogenic induction of bone substitute materials.

The effect of composition of calcium phosphate composite scaffolds on the formation of tooth tissue from human dental pulp stem cells

Biomaterials ◽  
2011 ◽  
Vol 32 (29) ◽  
pp. 7053-7059 ◽  
Author(s):  
Liqin Zheng ◽  
Fei Yang ◽  
Hong Shen ◽  
Xuefeng Hu ◽  
Chihiro Mochizuki ◽  
...  
Keyword(s):  
Stem Cells ◽  
Calcium Phosphate ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Composite Scaffolds ◽  
Human Dental Pulp ◽  
Tooth Tissue

scholarly journals Nanotopological-tailored calcium phosphate cements for the odontogenic stimulation of human dental pulp stem cells through integrin signaling

RSC Advances ◽  
2015 ◽  
Vol 5 (78) ◽  
pp. 63363-63371 ◽  
Author(s):  
So-Youn Lee ◽  
Hyung-Mun Yun ◽  
Roman A. Perez ◽  
Sara Gallinetti ◽  
Maria-Pau Ginebra ◽  
...  
Keyword(s):  
Stem Cells ◽  
Calcium Phosphate ◽  
Dental Pulp ◽  
Integrin Signaling ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Calcium Phosphate Cements ◽  
Human Dental Pulp ◽  
Pulp Cells ◽  
Stimulation Of

Nanotopological-tailored calcium phosphate cements stimulate odontogenesis of human dental pulp cells through integrin-mediated signaling pathways.

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