scholarly journals Histological Comparison of Autogenous and Allogenic Demineralized Dentin Matrix Loaded with Recombinant Human Bone Morphogenetic Protein-2 for Alveolar Bone Repair: A Preliminary Report

2017 ◽  
Vol 26 (4) ◽  
pp. 417-424 ◽  
Author(s):  
In-Woong Um ◽  
Sang-Ho Jun ◽  
Pil-Young Yun ◽  
Young-Kyun Kim
Keyword(s):  
Preliminary Report ◽  
Bone Repair ◽  
Alveolar Bone ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein ◽  
Human Bone Morphogenetic Protein ◽  
Dentin Matrix ◽  
Demineralized Dentin Matrix ◽  
Recombinant Human Bone ◽  
Demineralized Dentin

scholarly journals Volumetric, Radiographic, and Histologic Analyses of Demineralized Dentin Matrix Combined with Recombinant Human Bone Morphogenetic Protein-2 for Ridge Preservation: A Prospective Randomized Controlled Trial in Comparison with Xenograft

2018 ◽  
Vol 8 (8) ◽  
pp. 1288 ◽  
Author(s):  
Gyu-Un Jung ◽  
Tae-Hyun Jeon ◽  
Mong-Hun Kang ◽  
In-Woong Um ◽  
In-Seok Song ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Bone Morphogenetic Protein 2 ◽  
Ridge Preservation ◽  
Tissue Volume ◽  
Morphogenetic Protein ◽  
Human Bone Morphogenetic Protein ◽  
Dentin Matrix ◽  
Demineralized Dentin Matrix ◽  
Recombinant Human Bone ◽  
Demineralized Dentin

The aim of this study was to evaluate the clinical, volumetric, radiographic, and histologic aspects of autogenous demineralized dentin matrix (DDM) combined with recombinant human bone morphogenetic protein-2 (rhBMP-2) used for ridge preservation, compared to those of deproteinized bovine bone with collagen (DBBC). Following atraumatic extraction, the socket was filled with DBBC, DDM, or rhBMP-2/DDM. Scanned images of dental casts and cone beam computed tomographs (CBCT) were superimposed for the calculation of soft and hard tissue volume alteration. Preoperative and postoperative measurements of the height and width of the alveolar ridge were compared using CBCT images. After 4 months, bone specimens were harvested for histomorphometric assessment. Loss of hard and soft tissue volume occurred at 4 months after extraction and ridge preservation in all groups. No volumetric differences were detected among the three groups before and 4 months after ridge preservation. The reduction in the horizontal width at 5 mm was higher in the DBBC compared to the DDM. Histologically, approximately 40% newly formed bone was founded in rhBMP-2/DDM group. The autogenous dentin matrix used to fill the socket was as beneficial for ridge preservation as conventional xenografts. The combination of rhBMP-2 with dentin matrix also demonstrated appreciable volumetric stability and higher new bone formation compared to DDM alone and DBBC.

scholarly journals Bone regeneration of demineralized dentin matrix with platelet-rich fibrin and recombinant human bone morphogenetic protein-2 on the bone defects in rabbit calvaria

2021 ◽  
Vol 43 (1) ◽  
Author(s):  
Beom-Jin Kim ◽  
Seok-Kon Kim ◽  
Jae-Hoon Lee
Keyword(s):  
Bone Formation ◽  
Bone Defects ◽  
Bone Morphogenetic Protein 2 ◽  
New Bone Formation ◽  
Morphogenetic Protein ◽  
Human Bone Morphogenetic Protein ◽  
Dentin Matrix ◽  
Demineralized Dentin Matrix ◽  
Recombinant Human Bone ◽  
Demineralized Dentin

Abstract Background This study was to evaluate the bone formation ability of demineralized dentin matrix (DDM) combined with platelet-rich fibrinogen (PRF) and DDM combined with recombinant human bone morphogenetic protein-2 (rhBMP-2) to improve the osteoinductive ability of DDM. Methods After four bone defects with a diameter of 8mm were created in the calvarium of each rabbit, DDM was grafted into the first defect (experimental groups 1), a combination of DDM and PRF was grafted into the second defect (experimental groups 2), and DDM with absorbed rhBMP-2 was grafted into the third defect (experimental groups 3). The fourth defect was used as the control group. Twelve healthy male rabbits (New Zealand, white rabbits) weighing around 3.0–4.0 kg were used. Among 12 rabbits, 3 rabbits were sacrificed immediately after surgery and at 2, 4, and 8 weeks after surgery, respectively. Histopathologic analysis and histomorphometric analysis were conducted to evaluate bone formation in each group. Results The PRF/DDM group did not show a significantly higher degree of new bone formation in calvarial bone defects than the DDM group at 2, 4, and 8 weeks postoperatively in histopathological findings and histomorphometric results. On the other side, the rhBMP-2/DDM group showed higher degrees of new bone formation and calcification, and the lamellae of bone matrix, which are observed in mature bone tissue, were more distinctly visible in the rhBMP-2/DDM group. Moreover, the rhBMP-2/DDM group showed a significantly higher amount of new bone formation, compared to the DDM group at 4 and 8 weeks postoperatively (P<0.05) in histomorphometric results. Conclusion The DDM has great potential as a carrier for the maintenance and sustained release of rhBMP-2, which has been recently receiving wide attention as a type of signaling molecules to promote bone formation.

Tooth Eruption into the Newly Generated Bone Induced by Recombinant Human Bone Morphogenetic Protein-2

2002 ◽  
Vol 39 (4) ◽  
pp. 449-456 ◽  
Author(s):  
Atsushi Kitamura ◽  
Nobuyoshi Motohashi ◽  
Tatsuo Kawamoto ◽  
Yoshiyuki Baba ◽  
Shoichi Suzuki ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Alveolar Bone ◽  
Treated Group ◽  
Tooth Eruption ◽  
Human Bone ◽  
Bone Morphogenetic Protein 2 ◽  
Mineral Density ◽  
Morphogenetic Protein ◽  
Human Bone Morphogenetic Protein ◽  
Recombinant Human Bone

Objective The purpose of this study was to observe tooth eruption pattern into the newly generated bone induced by recombinant human bone morphogenetic protein-2 (rhBMP-2). Method Three 12-week-old beagle dogs were used. Following wound healing of the extracted maxillary third deciduous incisor at the age of 13 weeks, a resection of maxillary alveolar bone (8 mm by 8 mm by 8 mm) was carried out, accompanied by extraction of the maxillary second incisor tooth germ. The rhBMP-2 (5 μg/100 μL or 10 μg/100 μL) was implanted in the resected area. A group that was not implanted after resection and a nonresected group were used as controls. Serial changes of the eruption pattern of the maxillary third incisor in the implanted area were observed radiographically. Oxytetracycline and calcein were employed as bone markers. Nine weeks after the implantation, the animals were sacrificed. The samples were observed histologically and analyzed using peripheral quantitative computerized tomography to acquire the bone mineral density (BMD). Results In the rhBMP-2 (5 μg/100 μL)-treated group, histological findings and the BMD of the newly generated bone were almost same as in the non-resected group. The maxillary third incisor erupted through the implanted area. However, in the rhBMP-2 (10 μg/100 μL)-treated group, more osteoinductive activity and significantly higher BMD were observed in the newly generated bone. The maxillary third incisor erupted mesially so as to avoid this implanted area. Conclusion Results showed that bone remodeling and tooth eruption through the newly generated bone appear to be dose dependent. Accordingly, proper dosage of rhBMP-2 should be determined for successful clinical application.

Effects of the combination of low-level laser irradiation and recombinant human bone morphogenetic protein-2 in bone repair

2011 ◽  
Vol 27 (5) ◽  
pp. 971-977 ◽  
Author(s):  
Anderson Paim Rosa ◽  
Luiz Gustavo de Sousa ◽  
Simone Cecilio Hallak Regalo ◽  
João Paulo Mardegan Issa ◽  
Ana Paula Amorim Barbosa ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Laser Irradiation ◽  
Bone Repair ◽  
Human Bone ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein ◽  
Level Laser ◽  
Human Bone Morphogenetic Protein ◽  
Low Level Laser Irradiation ◽  
Recombinant Human Bone
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