scholarly journals Human Amniotic Membrane Extracts have Anti-Inflammatory Effects on Damaged Human Corneal Epithelial Cells In Vitro

2016 ◽  
Vol 25 (3) ◽  
pp. 282-287 ◽  
Author(s):  
Han-Na Lee ◽  
Raymundo Bernardo ◽  
Gi-Yeon Han ◽  
Gyu-Young Kim ◽  
Ji-Soo Kim ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Amniotic Membrane ◽  
Human Amniotic Membrane ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Human Corneal Epithelial Cells ◽  
Anti Inflammatory

scholarly journals Anandamide Concentration-Dependently Modulates Toll-Like Receptor 3 Agonism or UVB-Induced Inflammatory Response of Human Corneal Epithelial Cells

2021 ◽  
Vol 22 (15) ◽  
pp. 7776
Author(s):  
Ágnes Angyal ◽  
Zsófia Pénzes ◽  
Shahrzad Alimohammadi ◽  
Dorottya Horváth ◽  
Lili Takács ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Inflammatory Response ◽  
Toll Like Receptor ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Uvb Irradiation ◽  
Human Corneal Epithelial Cells ◽  
Anti Inflammatory

Photodamage-induced and viral keratitis could benefit from treatment with novel nonsteroid anti-inflammatory agents. Therefore, we determined whether human corneal epithelial cells (HCECs) express members of the endocannabinoid system (ECS), and examined how the endocannabinoid anandamide (AEA, N-arachidonoyl ethanolamine) influences the Toll-like receptor 3 (TLR3) agonism- or UVB irradiation-induced inflammatory response of these cells. Other than confirming the presence of cannabinoid receptors, we show that endocannabinoid synthesizing and catabolizing enzymes are also expressed in HCECs in vitro, as well as in the epithelial layer of the human cornea in situ, proving that they are one possible source of endocannabinoids. p(I:C) and UVB irradiation was effective in promoting the transcription and secretion of inflammatory cytokines. Surprisingly, when applied alone in 100 nM and 10 μM, AEA also resulted in increased pro-inflammatory cytokine production. Importantly, AEA further increased levels of these cytokines in the UVB model, whereas its lower concentration partially prevented the transcriptional effect of p(I:C), while not decreasing the p(I:C)-induced cytokine release. HCECs express the enzymatic machinery required to produce endocannabinoids both in vitro and in situ. Moreover, our data show that, despite earlier reports about the anti-inflammatory potential of AEA in murine cornea, its effects on the immune phenotype of human corneal epithelium may be more complex and context dependent.

scholarly journals Anti-inflammatory effects of hinokitiol on human corneal epithelial cells: an in vitro study

Eye ◽  
2015 ◽  
Vol 29 (7) ◽  
pp. 964-971 ◽  
Author(s):  
J Ye ◽  
Y-F Xu ◽  
L-X Lou ◽  
K Jin ◽  
Q Miao ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
In Vitro Study ◽  
Vitro Study ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Human Corneal Epithelial Cells ◽  
Anti Inflammatory

scholarly journals Biocompatibility of Nanocellulose-Reinforced PVA Hydrogel with Human Corneal Epithelial Cells for Ophthalmic Applications

2019 ◽  
Vol 10 (3) ◽  
pp. 35 ◽  
Author(s):  
Tummala ◽  
Lopes ◽  
Mihranyan ◽  
Ferraz
Keyword(s):  
Epithelial Cells ◽  
Cell Viability ◽  
Protein Adsorption ◽  
Direct Contact ◽  
Corneal Epithelial Cell ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Human Corneal Epithelial Cells ◽  
Hydrogel Surface

Transparent composite hydrogel in the form of a contact lens made from poly(vinyl alcohol) (PVA) and cellulose nanocrystals (CNCs) was subjected to in vitro biocompatibility evaluation with human corneal epithelial cells (HCE-2 cells). The cell response to direct contact with the hydrogels was investigated by placing the samples on top of confluent cell layers and evaluating cell viability, morphology, and cell layer integrity subsequent to 24 h culture and removal of the hydrogels. To further characterize the lens–cell interactions, HCE-2 cells were seeded on the hydrogels, with and without simulated tear fluid (STF) pre-conditioning, and cell viability and morphology were evaluated. Furthermore, protein adsorption on the hydrogel surface was investigated by incubating the materials with STF, followed by protein elution and quantification. The hydrogel material was found to have affinity towards protein adsorption, most probably due to the interactions between the positively charged lysozyme and the negatively charged CNCs embedded in the PVA matrix. The direct contact experiment demonstrated that the physical presence of the lenses did not affect corneal epithelial cell monolayers in terms of integrity nor cell metabolic activity. Moreover, it was found that viable corneal cells adhered to the hydrogel, showing the typical morphology of epithelial cells and that such response was not influenced by the STF pre-conditioning of the hydrogel surface. The results of the study confirm that PVA-CNC hydrogel is a promising ophthalmic biomaterial, motivating future in vitro and in vivo biocompatibility studies.

Sign in / Sign up

Export Citation Format

Share Document