scholarly journals Immunohistochemical Study of the Goat Ductus deferens

2018 ◽  
Vol 62 (1) ◽  
pp. 11-17
Author(s):  
E. Marettová ◽  
M. Maretta
Keyword(s):  
Smooth Muscle ◽  
Positive Reaction ◽  
Immunohistochemical Study ◽  
Smooth Muscle Actin ◽  
Elastic Fibers ◽  
Cytokeratin 18 ◽  
Ductus Deferens ◽  
Blood Capillaries ◽  
Tunica Adventitia ◽  
Tunica Muscularis

Abstract Ductus deferens plays an important role in sperm transport and participates in the preservation of structure, maturation, and viability of sperm. In this study, we have immunohistochemically examined the ductus deferens in the goat. For immunohistochemical study the following monoclonal antibodies were used: cytokeratin 18, α-smooth muscle actin (α-SMA), vimentin and elastin. Morphologically, three distinct layers were identified in the goat ductus deferens — tunica mucosa, tunica muscularis and tunica adventitia. The epithelium of the mucosa was intensely stained with cytokeratin 18 (CK 18). The fibroblasts in the lamina propria and blood capillaries in the muscle layer showed positive reaction for vimentin. A positive reaction for α-SMA was observed in the smooth muscle cells of the tunica muscularis in the internal, middle and outer sublayers. An intense positive reaction for α-SMA was observed in the wall of the blood vessels. Elastic fibers in the form of a loose meshwork were present in all three layers. The high density of elastic fibers were found in the tunica adventitia.

scholarly journals Expression Of Cytokeratin 18 In The Peripheral Nerves

2016 ◽  
Vol 60 (2) ◽  
pp. 5-10
Author(s):  
E. Marettová
Keyword(s):  
Skeletal Muscle ◽  
Smooth Muscle ◽  
Epithelial Cells ◽  
Peripheral Nerves ◽  
Cytokeratin 18 ◽  
Ductus Deferens ◽  
Nerve Sheath ◽  
Myelinated Nerves ◽  
Perineurial Cells ◽  
Perineurial Sheath

Abstract The perineurium constitutes the basis for the regulation of endoneurial fluid homeostasis. In the work presented here, cytokeratin 18, as an immunohistochemical marker for epithelial cells, was used to identify the perineurium in the peripheral nerves of two species. Two organs, rich in peripheral nerves, were used; the tongue of the bull and the ductus deferens of the male goat. Special attention was paid to one of the the nerve sheath cells - the perineurial cells of myelinated nerves in the skeletal muscle of the tongue and in the smooth muscle in the wall of the ductus deferens. A positive reaction to cytokeratin 18 was found in the perineurial cells of the perineurial sheath in the nerves of various sizes. No difference in the reactivity was observed between the peripheral nerves of the tongue and that of the ductus deferens.

scholarly journals Non-metastatic ileal leiomyosarcoma in an aged pouter pigeon (Columba livia)

2018 ◽  
Vol 68 (4) ◽  
pp. 675
Author(s):  
CD GORNATTI CHURRIA ◽  
P LOUKOPOULOS
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Actin ◽  
Columba Livia ◽  
Avian Species ◽  
Intestinal Smooth Muscle ◽  
Muscle Actin ◽  
First Report ◽  
Spindle Cells ◽  
Tunica Muscularis ◽  
Antiparasitic Treatment

Intestinal smooth muscle neoplasms are rarely reported in avian species. An aged male pouter pigeon (Columba livia) had anorexia, somnolence, progressive emaciation and yellowish diarrhea of four days’ duration and died despite antibiotic and antiparasitic treatment. At necropsy, the ileum bore a whitish, nodular, 0.8 cm in diameter mass severely narrowing the lumen. Histopathologically, the ileal mass was a well-demarcated, expansile neoplasm severely expanding the tunica muscularis and invading the mucosa. The neoplasm was composed of densely packed spindle cells with elongated nuclei arranged in broad interlacing bundles, and showing mild anisocytosis and anisokaryosis. Immunohistochemically, the neoplasm was positive for desmin and smooth muscle actin, and negative for cytokeratin, and c-kit. The gross, histopathologic and immunohistochemical findings provided the diagnosis of ileal leiomyosarcoma. This is the first report of an ileal leiomyosarcoma in Columbiformes and one of few intestinal smooth muscle neoplasms described in birds.

scholarly journals Occurrence of an Abdominal Leiomyosarcoma in the Cyprinus carpio: Optical and Immunohistochemical Study

2021 ◽  
pp. 10-19
Author(s):  
Luis Alberto Romano ◽  
Virgínia Fonseca Pedrosa
Keyword(s):  
Cell Proliferation ◽  
Smooth Muscle ◽  
Immunohistochemical Study ◽  
Histopathological Examination ◽  
Smooth Muscle Actin ◽  
Swim Bladder ◽  
Muscle Actin ◽  
Immunohistochemical Examination ◽  
Vortex Pattern ◽  
Spindle Cells

A capsulated nodular neoplasm measuring 9 cm in diameter, located between the swim bladder and the kidney sample of Cyprinuscarpio, was in laboratory. The neoplasm had a solid consistency in one sector and very adherent to the capsule. Histopathological examination of the neoplasm revealed a monotonous proliferation of spindle cells, and pleomorphic, sometimes in a vortex pattern and interspersed with collagen fibers. Cells had numerous well-oriented myofibrils giving them a deep red. The cytoplasm is eosinophilic and the nuclei are hyperchromatic located in the center with blunt or "cigar-shaped" ends. In the immunohistochemical examination, the tumor cells were positive for desmin, smooth muscle actin and K-47, the latter antibody showing significant cell proliferation. Due to histopathological and immunohistochemical findings, diagnosis was made with leiomyosarcoma.

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