scholarly journals Effects of Carbon and Nitrogen Sources on Activity of Cell Envelope Proteinase Produced by Lactobacillus plantarum LP69

2019 ◽  
Vol 23 (1) ◽  
pp. 11-18
Author(s):  
Fangfang Cheng ◽  
He Chen ◽  
Ni Lei ◽  
Meng Zhang ◽  
Hongchang Wan
Keyword(s):  
Lactobacillus Plantarum ◽  
Specific Activity ◽  
Cell Envelope ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Soluble Starch ◽  
Carbon And Nitrogen Sources ◽  
Individual Factor ◽  
Carbon And Nitrogen ◽  
Mrs Broth

Abstract In present study, the effects of carbon sources (glucose, lactose, sucrose, galactose, maltose and soluble starch) and nitrogen sources (casein peptone, whey protein, soy peptone, yeast, tryptone, beef extract and peptone) on activity of cell envelope proteinases (CEP), specific activity, protein content, OD600 value and pH in MRS broth fermented by Lactobacillus plantarum LP69 were investigated by individual factor experiment. The results indicated that carbon and nitrogen sources have significant influence on the activity of CEP and specific activitys of L. plantarum LP69, glucose, maltose, casein peptone and peptone are superior to other selected carbon and nitrogen sources. The optimum concentrations of glucose, maltose, casein peptone and peptone for L. plantarum LP69 are 2%, 2%, 1% and 1%; the activity of CEP are 19.52U/mL, 21.13U/mL, 13.49U/mL and 20.61U/mL, respectively.

Anoxybacillus sp. AH1, an α-amylase-producing thermophilic bacterium isolated from Dargeçit hot spring

Biologia ◽  
2015 ◽  
Vol 70 (7) ◽  
Author(s):  
Ömer Acer ◽  
Hemşe Pırınççiğlu ◽  
Fatma Matpan Bekler ◽  
Reyhan Gül-Güven
Keyword(s):  
Hot Springs ◽  
Hot Spring ◽  
Thermophilic Bacteria ◽  
Potato Starch ◽  
Nitrogen Sources ◽  
Soluble Starch ◽  
Thermophilic Bacterium ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Optimum Growth Temperature

AbstractThe present study was conducted to isolate α-amylase-producing thermophilic bacteria from Darge¸cit hot springs in Turkey. The morphological, biochemical and physiological characterisation, as well as genetic analysis by 16S rRNA sequences indicated that the isolated strain AH1 was a member of Anoxybacillus genus. The strain was aerobe, Gram-positive and spore-forming rod, exhibiting optimum growth temperature and pH of 60ºC and 7.0-7.5, respectively. Optimization of growth medium and enzyme assay conditions for extracellular α-amylase production by the novel thermophilic Anoxybacillus sp. AH1 were carried out in many different media containing a variety of carbon and nitrogen sources. Among various carbon and nitrogen sources, peptone (2054.1 U/mL) at 1% and maltose (1862.9 U/mL) at 0.5% increased α-amylase activity, compared to controls. Moreover, a high enzyme production was observed with potato starch at 0.5% and 1% (2668.4 U/mL and 3627 U/mL, respectively), as well as with 1% soluble starch (2051.9 U/mL). The enzyme activity was found to be rather high in the presence of CaCl

scholarly journals Effects of carbon and nitrogen sources on production of proteases by Bacillus subtilis IC-5

2018 ◽  
Vol 44 (2) ◽  
pp. 285-292
Author(s):  
Sereen Gul ◽  
Mujeeb Ur Rahman ◽  
Mohammad Ajmal ◽  
Abdul Kabir Khan Achakzai ◽  
Asim Iqbal
Keyword(s):  
Bacillus Subtilis ◽  
Sodium Nitrate ◽  
Inorganic Nitrogen ◽  
Carbon Sources ◽  
Basal Medium ◽  
Nitrogen Sources ◽  
Neutral Protease ◽  
Carbon And Nitrogen Sources ◽  
Inorganic Nitrogen Source ◽  
Carbon And Nitrogen

The effects of various carbon and nitrogen sources were evaluated on production of proteases by Bacillus subtilis IC-5. Both type and concentration of carbon and nitrogen sources influenced the production of proteases. Among the carbon sources glucose was found to be the most effective. It gave maximum production at 2% w/v concentration i.e., 1875 and 950 U/ml, alkaline and neutral protease, respectively. The response of Bacillus subtilis IC-5 towards synthesis and excretion of enzymes varied with the type of nitrogen sources. The addition of organic nitrogen sources to basal medium repressed the synthesis of proteases while the addition of inorganic nitrogen source such as sodium nitrate was found to be the best stimulating for alkaline and neutral protease synthesis. Sodium nitrate enhanced the production up to 62.40 and 10.52% of alkaline and neutral protease, respectively against w.r.t. control.

Study of the specific activity of protein hydrolysates regarding test strains used to control of nutrient media

2017 ◽  
pp. 41-43
Author(s):  
Yu.S. Kovtun ◽  
A.A. Kurilova ◽  
L.S. Katunina
Keyword(s):  
Vibrio Cholerae ◽  
Streptococcus Pyogenes ◽  
Fish Meal ◽  
Specific Activity ◽  
Nitrogen Sources ◽  
Bovine Blood ◽  
Nutrient Media ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Agar Media

An evaluation of the specific activity towards the test strains of Streptococcus pyogenes Dick I, Vibrio cholerae non O1 P-9741 agar media containing, pancreatic hydrolysates of gelatin, soy, soy concentrate, maize gluten, fish meal, common kilka, bovine blood as carbon and nitrogen sources was made. The variations in the number, size and frequency of dissociation among the colonies of these test strains were discovered, which allow to develop a differentiated approach to the possible usage of studied hydrolysates.

scholarly journals Characterization of Diazotrophic Rhizobacteria under Various Conditions

2013 ◽  
Vol 1 (3) ◽  
pp. 110-117
Author(s):  
Umesh Prasad Shrivastava
Keyword(s):  
Nitrogenase Activity ◽  
Nifh Gene ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Accession Number ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Reduction Assay ◽  
Assay Methods

Nitrogenase activity was analysed after supplementation of various carbon and nitrogen sources in the growth medium by Acetylene Reduction Assay methods in selected 9 isolates from 74 diazotrophic isolates.  Enhancement in nitrogenase activity was recorded many fold by the addition of different organic carbon sources in which maltose and pyruvate showed better result than others. In case of supplementation of nitrogen sources, reduction of nitrogenase activity was observed.  Nitrogenase activity increased from 22.7 to 72.7% in various strains when they are tested in anaerobic condition, Amplification of fragment of 390 bp showed that nitrogenase activity due to presence of nifH gene.  Sequences were submitted to NCBI GeneBank and the accession number of nifH sequence of ECI-10A (FJ032023), AF-4B (FJ032024), AF-4C (FJ032022) and BN-2A (FJ032021) has been obtained. Phylogenetic analysis based on showed that these 4 isolates belong to the member of γ-proteobacteria, but show appreciable genetic diversity.DOI: http://dx.doi.org/10.3126/ijasbt.v1i3.8607 Int J Appl Sci Biotechnol, Vol. 1(3) 2013 : 110-117

scholarly journals Yarrowia lipolyticaand Its Multiple Applications in the Biotechnological Industry

2014 ◽  
Vol 2014 ◽  
pp. 1-14 ◽  
Author(s):  
F. A. G. Gonçalves ◽  
G. Colen ◽  
J. A. Takahashi
Keyword(s):  
Carbon Sources ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen Sources ◽  
Biotechnological Potential ◽  
Carbon And Nitrogen ◽  
Intracellular Lipids ◽  
Metabolites Production ◽  
Growing Conditions ◽  
Fermentation Parameters

Yarrowia lipolyticais a nonpathogenic dimorphic aerobic yeast that stands out due to its ability to grow in hydrophobic environments. This property allowed this yeast to develop an ability to metabolize triglycerides and fatty acids as carbon sources. This feature enables using this species in the bioremediation of environments contaminated with oil spill. In addition,Y. lipolyticahas been calling the interest of researchers due to its huge biotechnological potential, associated with the production of several types of metabolites, such as bio-surfactants,γ-decalactone, citric acid, and intracellular lipids and lipase. The production of a metabolite rather than another is influenced by the growing conditions to whichY. lipolyticais subjected. The choice of carbon and nitrogen sources to be used, as well as their concentrations in the growth medium, and the careful determination of fermentation parameters, pH, temperature, and agitation (oxygenation), are essential for efficient metabolites production. This review discusses the biotechnological potential ofY. lipolyticaand the best growing conditions for production of some metabolites of biotechnological interest.

scholarly journals Response of Cold-Tolerant Aspergillus spp. to Solubilization of Fe and Al Phosphate in Presence of Different Nutritional Sources

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
K. Rinu ◽  
Mukesh Kumar Malviya ◽  
Priyanka Sati ◽  
S. C. Tiwari ◽  
Anita Pandey
Keyword(s):  
Phosphate Solubilization ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Iron Phosphate ◽  
Fungal Species ◽  
Growth Promoters ◽  
Aluminium Phosphate ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Phosphate Solubilizing

Three species of Aspergillus, namely, A. niger, A. glaucus and A. sydowii, isolated from soil samples collected from the Indian Himalayan Region (IHR), have been investigated for solubilization of aluminium phosphate and iron phosphate in the presence of different carbon and nitrogen sources. Preference of each fungal species varied for nitrogen and carbon sources, in terms of phosphate-solubilization. Among three species, Aspergillus niger gave the best results; it solubilized 32% and 8% of the supplemented aluminium phosphate and iron phosphate, respectively. The results indicated that the effect of carbon and nitrogen sources can influence the phosphate solubilizing efficiency of all the three Aspergillus spp. tested. All the three species were found to be plant-growth promoters in bioassays conducted under greenhouse conditions. The Al and Fe phosphate solubilization efficiency, investigated in the present study, is at the lower end of their previously reported tricalcium phosphate solubilization efficiency. The cultures are likely to have better field applications in agrobiotechnology, due to their potential towards solubilization of Al and Fe phosphates, which are known to have lower solubility through microbial activity.

A COMPARISON OF ISOLATES OF CLAVICEPS SPP. FOR THE ABILITY TO GROW AND TO PRODUCE ERGOT ALKALOIDS ON CERTAIN NUTRIENTS

1960 ◽  
Vol 6 (3) ◽  
pp. 355-365 ◽  
Author(s):  
W. A. Taber ◽  
L. C. Vining
Keyword(s):  
Carbon Source ◽  
Carbon Sources ◽  
Ergot Alkaloids ◽  
Nitrogen Sources ◽  
Claviceps Purpurea ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Different Strains ◽  
Do So

Isolates of Claviceps purpurea and Claviceps spp. obtained from various geographical areas were compared for their ability to grow and to produce ergot alkaloids in vitro on various carbon and nitrogen sources. While some differences in utilization of carbon sources for growth were found, there was no observed correlation between utilization of carbohydrates and the capacity to produce ergot alkaloids. The amount of alkaloid produced by different strains depended upon both the carbon and nitrogen sources. In general, those cultures capable of alkaloid production were able to do so on more than one carbon source, but the carbon source allowing greatest production differed from one strain to another. Both producing and non-producing strains could utilize succinic acid as a carbon source for growth, but neither could utilize L-tryptophane as a carbon or nitrogen source for growth.

scholarly journals Production, purification and characterization of thermostable alpha amylase from Bacillus subtilis Y25 isolated from decaying yam (Dioscorea rotundata) tuber

2020 ◽  
Vol 12 (1) ◽  
pp. 154-171
Author(s):  
Oluwatoyin M. ALADEJANA ◽  
Olaoluwa OYEDEJI ◽  
Olumide OMOBOYE ◽  
Mufutau BAKARE
Keyword(s):  
Bacillus Subtilis ◽  
Metal Ion ◽  
Specific Activity ◽  
Nitrogen Sources ◽  
16S Rrna Gene Sequencing ◽  
Exchange Chromatography ◽  
Rrna Gene ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Amylase Production

Amylases have wide biotechnological potentials for applications in various industries. An α-amylase-producing bacterium was isolated from deteriorating yam tubers. Molecular characterization using the 16S rRNA gene sequencing was used to confirm the identity of the bacterium as Bacillus subtilis Y25. The effect of some cultural and nutritional factors such as pH, temperature, carbon and nitrogen sources on α-amylase production from the bacterium was determined. Maximum α-amylase production was observed using starch and peptone as carbon and nitrogen sources, respectively, with an initial medium pH of 8.0 and incubation at 45 °C for 36 h. The enzyme was purified by ion exchange chromatography on CM Sepharose CL-6B. The kinetic parameters Km and Vmax of the enzyme, as well as the effect of pH, temperature, metal ions and ethylenediaminetetra acetic acid (EDTA) on the activity of the purified enzyme were studied. The specific activity of the partially purified enzyme was determined to be 15.21 Units/mg protein with a purification fold of 3.80. The molecular weight of the purified enzyme was estimated to be 58.0 kDa. The Vmax and Km values obtained with soluble starch for Bacillus subtilis Y25 α-amylase were 314.10 ± 23.30 Units/mg protein and 53.98 ± 12.03 mg/ml, respectively. The enzyme exhibited optimum activity at a temperature of 60 °C and pH 8.0. The metal ion Ca2+ had no effect on the enzyme at 20 mM concentration, whereas Na+ and Mg2+, as well as EDTA inhibited the enzyme at the same concentration. The characteristics of the α-amylase from Bacillus subtilis Y25 revealed it to be a thermostable and an alkaline metalloenzyme with potential for applications in the detergent and saccharification industries.

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