A COMPARISON OF ISOLATES OF CLAVICEPS SPP. FOR THE ABILITY TO GROW AND TO PRODUCE ERGOT ALKALOIDS ON CERTAIN NUTRIENTS

1960 ◽  
Vol 6 (3) ◽  
pp. 355-365 ◽  
Author(s):  
W. A. Taber ◽  
L. C. Vining
Keyword(s):  
Carbon Source ◽  
Carbon Sources ◽  
Ergot Alkaloids ◽  
Nitrogen Sources ◽  
Claviceps Purpurea ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Different Strains ◽  
Do So

Isolates of Claviceps purpurea and Claviceps spp. obtained from various geographical areas were compared for their ability to grow and to produce ergot alkaloids in vitro on various carbon and nitrogen sources. While some differences in utilization of carbon sources for growth were found, there was no observed correlation between utilization of carbohydrates and the capacity to produce ergot alkaloids. The amount of alkaloid produced by different strains depended upon both the carbon and nitrogen sources. In general, those cultures capable of alkaloid production were able to do so on more than one carbon source, but the carbon source allowing greatest production differed from one strain to another. Both producing and non-producing strains could utilize succinic acid as a carbon source for growth, but neither could utilize L-tryptophane as a carbon or nitrogen source for growth.

scholarly journals Influence of cultivation conditions on IAA - producing activity of endophytic bacterial strains isolated from morning-glory (IPOMOEA PES-CAPRAE (L.) R.Br.)

2021 ◽  
Vol 36 ◽  
pp. 05003
Author(s):  
Nguyen Van Zhang ◽  
Nguyen Thi Thu ◽  
Vu Thi Linh ◽  
V.V. Pylnev ◽  
M.I. Popchenko
Keyword(s):  
Carbon Source ◽  
Culture Medium ◽  
Nitrogen Sources ◽  
Morning Glory ◽  
Bacterial Strains ◽  
Cultivation Conditions ◽  
Bacillus Mycoides ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Study Results

This work presents the experimental study results of the influence of the culture medium on the ability to IAA synthesis of three endophytic strains TH10R, TH11T, and TH13T from roots of Ipomoea pes-caprae. Three investigated strains give the highest IAA concentration after 96 h of cultivation. A significant increase in IAA biosynthesis was obtained by cultivating the TH10R strain in a medium containing lactose or starch as a carbon source and NH4Cl or KNO3 as a nitrogen source. The TH11T strain produces the maximum amount of IAA, using glucose or xylose and KNO3 or NH4NO3 as carbon and nitrogen sources, respectively. Sucrose is a suitable carbon source for the TH13T strain; on a sucrose-containing medium, the TH13T strain produces the highest IAA amount. The most active strain is TH10R, identified as Bacillus mycoides and named Bacillus mycoides TH10R.

scholarly journals Lovastatin Biosynthesis by Aspergillus terreus in a Chemically Defined Medium

2001 ◽  
Vol 67 (6) ◽  
pp. 2596-2602 ◽  
Author(s):  
Hassan Hajjaj ◽  
Peter Niederberger ◽  
Philippe Duboc
Keyword(s):  
Carbon Source ◽  
Aspergillus Terreus ◽  
Glucose Repression ◽  
Nitrogen Sources ◽  
Glucose Consumption ◽  
Defined Medium ◽  
Specific Productivity ◽  
Chemically Defined Medium ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen

ABSTRACT Lovastatin is a secondary metabolite produced by Aspergillus terreus. A chemically defined medium was developed in order to investigate the influence of carbon and nitrogen sources on lovastatin biosynthesis. Among several organic and inorganic defined nitrogen sources metabolized by A. terreus, glutamate and histidine gave the highest lovastatin biosynthesis level. For cultures on glucose and glutamate, lovastatin synthesis initiated when glucose consumption levelled off. When A. terreus was grown on lactose, lovastatin production initiated in the presence of residual lactose. Experimental results showed that carbon source starvation is required in addition to relief of glucose repression, while glutamate did not repress biosynthesis. A threefold-higher specific productivity was found with the defined medium on glucose and glutamate, compared to growth on complex medium with glucose, peptonized milk, and yeast extract.

scholarly journals Effects of carbon and nitrogen sources on production of proteases by Bacillus subtilis IC-5

2018 ◽  
Vol 44 (2) ◽  
pp. 285-292
Author(s):  
Sereen Gul ◽  
Mujeeb Ur Rahman ◽  
Mohammad Ajmal ◽  
Abdul Kabir Khan Achakzai ◽  
Asim Iqbal
Keyword(s):  
Bacillus Subtilis ◽  
Sodium Nitrate ◽  
Inorganic Nitrogen ◽  
Carbon Sources ◽  
Basal Medium ◽  
Nitrogen Sources ◽  
Neutral Protease ◽  
Carbon And Nitrogen Sources ◽  
Inorganic Nitrogen Source ◽  
Carbon And Nitrogen

The effects of various carbon and nitrogen sources were evaluated on production of proteases by Bacillus subtilis IC-5. Both type and concentration of carbon and nitrogen sources influenced the production of proteases. Among the carbon sources glucose was found to be the most effective. It gave maximum production at 2% w/v concentration i.e., 1875 and 950 U/ml, alkaline and neutral protease, respectively. The response of Bacillus subtilis IC-5 towards synthesis and excretion of enzymes varied with the type of nitrogen sources. The addition of organic nitrogen sources to basal medium repressed the synthesis of proteases while the addition of inorganic nitrogen source such as sodium nitrate was found to be the best stimulating for alkaline and neutral protease synthesis. Sodium nitrate enhanced the production up to 62.40 and 10.52% of alkaline and neutral protease, respectively against w.r.t. control.

Effect of carbon and nitrogen sources, pH and temperature on in vitro culture of several isolates of Amanita caesarea (Scop.:Fr.) Pers.

Mycorrhiza ◽  
2005 ◽  
Vol 16 (2) ◽  
pp. 133-136 ◽  
Author(s):  
A. Daza ◽  
J. L. Manjón ◽  
M. Camacho ◽  
L. Romero de la Osa ◽  
A. Aguilar ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen

scholarly journals Characterization of Diazotrophic Rhizobacteria under Various Conditions

2013 ◽  
Vol 1 (3) ◽  
pp. 110-117
Author(s):  
Umesh Prasad Shrivastava
Keyword(s):  
Nitrogenase Activity ◽  
Nifh Gene ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Accession Number ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Reduction Assay ◽  
Assay Methods

Nitrogenase activity was analysed after supplementation of various carbon and nitrogen sources in the growth medium by Acetylene Reduction Assay methods in selected 9 isolates from 74 diazotrophic isolates.  Enhancement in nitrogenase activity was recorded many fold by the addition of different organic carbon sources in which maltose and pyruvate showed better result than others. In case of supplementation of nitrogen sources, reduction of nitrogenase activity was observed.  Nitrogenase activity increased from 22.7 to 72.7% in various strains when they are tested in anaerobic condition, Amplification of fragment of 390 bp showed that nitrogenase activity due to presence of nifH gene.  Sequences were submitted to NCBI GeneBank and the accession number of nifH sequence of ECI-10A (FJ032023), AF-4B (FJ032024), AF-4C (FJ032022) and BN-2A (FJ032021) has been obtained. Phylogenetic analysis based on showed that these 4 isolates belong to the member of γ-proteobacteria, but show appreciable genetic diversity.DOI: http://dx.doi.org/10.3126/ijasbt.v1i3.8607 Int J Appl Sci Biotechnol, Vol. 1(3) 2013 : 110-117

scholarly journals Production of Phytase by three thermophilic fungi

2021 ◽  
Vol 23 (1) ◽  
Author(s):  
Ranjith Kumar R ◽  
Rajendar Reddy ◽  
Girisham S ◽  
Reddy S.M.
Keyword(s):  
Carbon Source ◽  
Nitrogen Sources ◽  
The Other ◽  
Thermomyces Lanuginosus ◽  
Thermophilic Fungi ◽  
Carbon And Nitrogen Sources ◽  
Phytase Production ◽  
Carbon And Nitrogen ◽  
Cultural Conditions ◽  
Other Hand

Production of phytase by three thermophilic fungi, Thermomyces lanuginosus, Talaromyces luteus and Rhizomucor pusillus under different cultural conditions was assessed. Temperature of 45°C, pH-6.0 were optimum for phytase production by the all three fungi under investigation . Carbon and nitrogen sources for production of phytases by the three thermophilic fungi varied with the fungus. When T. lanuginosus opted for D-glucose followed by D-fructose, T. luteus preferred D-glucose, D-mannose and mannitol for production a phytase. On the other hand, R. pusillus produced maximum phytase during its growth on mannitol and maltose as carbon source. L- asparagine, L- arginine and L-asparatic acid were preferred nitrogen sources for production of phytase by T. lanuginosus. On the other hand T. luteus, opted for L- asparagine, L-glutamic acid and L- glycine for the activity of phytase. R. pusillus produced maximum phytase in medium containing L-argine, L-asparagine and L- asparatic acid.

scholarly journals Xylariales): Physiological characterization and structural features of its association with wheat.

2018 ◽  
Vol 53 (2) ◽  
pp. 169-182
Author(s):  
Carolina Rothen ◽  
Victoria Miranda ◽  
Sebastián Fracchia ◽  
Alicia Godeas ◽  
Alejandra Rodríguez
Keyword(s):  
Root Hairs ◽  
Nitrogen Sources ◽  
Structural Features ◽  
Fungal Colonization ◽  
Carbon And Nitrogen Sources ◽  
Wheat Roots ◽  
Transmission Microscopy ◽  
Carbon And Nitrogen ◽  
Microdochium Bolleyi

: Plant roots can be colonized by asymptomatic fungal strains belonging to several taxa, among them, the group defined as Dark Septate Endophytes (DSE). Microdochium bolleyi commonly colonizes wheat roots and other crops. It is considered a weak pathogen or even a non-pathogenic fungal species, which has also been considered as a potential biocontrol agent against aggressive soil-borne pathogens in cereal crops. We isolated a strain of M. bolleyi from wheat roots sampled in a crop field in Argentina, and characterized its abilities to grow in different carbon and nitrogen sources, to produce indole and to solubilize phosphorus; also several enzymatic activities were evaluated. In addition, resynthesis was performed under controlled conditions in order to characterize root fungal colonization under both, optical and transmission microscopy. The strain 22-1 colonized wheat root parenchymal tissue, forming chlamysdospores inside parenchymal cells and root hairs, and poorly grew in carbon and nitrogen sources. This fungus also synthesized indoles in in vitro culture, but it cannot solubilize phosphorus. Only amylase activity was detected out of seven enzymatic activity measured. Microdochium bolleyi (strain 22-1) colonized the roots, it formed typical DSE fungal structures and behaved like a “true endophyte”; however further studies are necessary to elucidate its role in the association with wheat.

scholarly journals Yarrowia lipolyticaand Its Multiple Applications in the Biotechnological Industry

2014 ◽  
Vol 2014 ◽  
pp. 1-14 ◽  
Author(s):  
F. A. G. Gonçalves ◽  
G. Colen ◽  
J. A. Takahashi
Keyword(s):  
Carbon Sources ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen Sources ◽  
Biotechnological Potential ◽  
Carbon And Nitrogen ◽  
Intracellular Lipids ◽  
Metabolites Production ◽  
Growing Conditions ◽  
Fermentation Parameters

Yarrowia lipolyticais a nonpathogenic dimorphic aerobic yeast that stands out due to its ability to grow in hydrophobic environments. This property allowed this yeast to develop an ability to metabolize triglycerides and fatty acids as carbon sources. This feature enables using this species in the bioremediation of environments contaminated with oil spill. In addition,Y. lipolyticahas been calling the interest of researchers due to its huge biotechnological potential, associated with the production of several types of metabolites, such as bio-surfactants,γ-decalactone, citric acid, and intracellular lipids and lipase. The production of a metabolite rather than another is influenced by the growing conditions to whichY. lipolyticais subjected. The choice of carbon and nitrogen sources to be used, as well as their concentrations in the growth medium, and the careful determination of fermentation parameters, pH, temperature, and agitation (oxygenation), are essential for efficient metabolites production. This review discusses the biotechnological potential ofY. lipolyticaand the best growing conditions for production of some metabolites of biotechnological interest.

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