scholarly journals Effects of Low Seminal Plasma Antioxidant Potential on Semen Quality and Male Fertility

2019 ◽  
Vol 1 (1) ◽  
pp. 4-8
Author(s):  
Ayad Palani ◽  
Nora Asdallh
Keyword(s):  
Seminal Plasma ◽  
Male Fertility ◽  
Semen Quality ◽  
Antioxidant Potential ◽  
Plasma Antioxidant

scholarly journals Semen quality, lipid peroxidation, and seminal plasma antioxidant status in horses with different intensities of physical exercise

2013 ◽  
Vol 82 (1) ◽  
pp. 31-35 ◽  
Author(s):  
Helena Härtlová ◽  
Radko Rajmon ◽  
Iva Krontorádová ◽  
Jiří Mamica ◽  
Lukáš Zita ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Seminal Plasma ◽  
Aspartate Aminotransferase ◽  
Semen Quality ◽  
Antioxidant Activities ◽  
Membrane Damage ◽  
Antioxidant Enzyme Activities ◽  
Reference Ranges ◽  
Stress Symptoms ◽  
Plasma Antioxidant

The aim of this study was to compare markers of semen quality, sperm membrane damage, and the seminal plasma antioxidant activity in warmblood stallions with and without sport workload stress. Four stallions were used for breeding only (control) and four both for breeding and competition in jumping. Semen samples were collected at 14-day intervals (from June to August) from each stallion (5 ejaculates per stallion). Immediately after sperm collection, a conventional examination of the ejaculate was processed. Catalytic activities of enzymes aspartate aminotransferase, alanin aminotransferase, glutathione peroxidase, superoxide dismutase and indicator of lipoperoxidation - F2α isoprostanes were measured in samples of seminal plasma. Contrary to basic semen quality indicators, the values of seminal plasma pH, aspartate aminotransferase and alanin aminotransferase were significantly (P < 0.05) impaired in the physically stressed stallions. Also, the level of F2α isoprostanes and the activity of superoxide dismutase were significantly (P < 0.05) increased by stress. The antioxidant activities of superoxide dismutase and glutathion peroxidase increased during the monitored period and reflected changes in F2α isoprostane concentration. We can conclude that even the conventional basic sperm indicators stay within the reference ranges of the biochemical indicators of seminal plasma such as pH or AST/ALT activity may be negatively influenced by sport workload stress. Increased concentrations of F2α isoprostanes indicate that lipoperoxidation can be a mechanism of cell membrane destabilization, which is counteracted by an increase of antioxidant enzyme activities. This is the first report of oxidative stress symptoms in normospermic equine semen in relation to stallion sport workload.

Gelatinases and their tissue inhibitors are associated with oxidative stress: a potential set of markers connected with male infertility

2016 ◽  
Vol 28 (7) ◽  
pp. 1029 ◽  
Author(s):  
Ewa M. Kratz ◽  
Anna Kałuża ◽  
Mirosława Ferens-Sieczkowska ◽  
Beata Olejnik ◽  
Renata Fiutek ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Seminal Plasma ◽  
Artificial Insemination ◽  
Male Fertility ◽  
Semen Quality ◽  
Reproductive Potential ◽  
Advanced Oxidation Protein Products ◽  
Tissue Inhibitors ◽  
Total Antioxidant ◽  
Infertile Patients

The expression and activity of matrix metalloproteinases (MMPs) may be regulated by oxidative stress in various pathophysiological processes; therefore, the aim of the present study was to analyse the associations between the expression of the gelatinases MMP-9 and MMP-2 and their tissue inhibitors TIMP-1, TIMP-2 and levels of total antioxidant capacity (TAC) and advanced oxidation protein products (AOPP) in seminal plasma prepared for artificial insemination. Levels of MMPs and TIMPs were evaluated using ELISA, whereas TAC and AOPP in the seminal plasma of 131 childless men and 38 fertile volunteers were determined spectrophotometrically. Seminal MMP-9 expression was higher in childless men than in fertile subjects, whereas there was no significant differences in MMP-2 expression between the analysed seminal groups. TIMP-1 and TIMP-2 expression was similar in all groups. However, TAC expression was significantly higher in infertile normozoospermic and oligozoospermic men and AOPP expression was higher in astheno-, oligo- and normozoospermic infertile patients than in fertile men. High AOPP, together with an increased MMP-9 : TIMP-1 ratio alters the oxidative–antioxidative balance of the ejaculate, thereby reducing male fertility, and therefore these parameters may serve as additional diagnostic markers of semen quality and male reproductive potential.

First insights on seminal extracellular vesicles in chickens of contrasted fertility

Reproduction ◽  
2021 ◽  
Vol 161 (5) ◽  
pp. 489-498 ◽  
Author(s):  
Luiz Cordeiro ◽  
Hsiu-Lien Herbie Lin ◽  
Anaïs Vitorino Carvalho ◽  
Isabelle Grasseau ◽  
Rustem Uzbekov ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Seminal Plasma ◽  
Male Fertility ◽  
Semen Quality ◽  
Sperm Viability ◽  
Protein Markers ◽  
Fertility Status ◽  
Male Subfertility ◽  
Incubation Experiments ◽  
Size Characterization

Male subfertility causes are very varied and sometimes related to post-gonadic maturation disruption, involving seminal plasma constituents. Among them, extracellular vesicles are involved in key exchanges with sperm in mammals. However, in birds, the existence of seminal extracellular vesicles is still debated. The aim of the present work was first to clarify the putative presence of extracellular vesicles in the seminal plasma of chickens, secondly to characterize their size and protein markers in animals showing different fertility, and finally to make preliminary evaluations of their interactions with sperm. We successfully isolated extracellular vesicles from seminal plasma of males showing the highest differences in semen quality and fertility by using ultracentrifugation protocol (pool of 3 ejaculates/rooster, n =3/condition). Size characterization performed by electron microscopy revealed a high proportion of small extracellular vesicles (probably exosomes) in chicken seminal plasma. Smaller extracellular vesicles appeared more abundant in fertile than in subfertile roosters, with a mean diameter of 65.12 and 77.18 nm, respectively. Different protein markers of extracellular vesicles were found by western blotting (n = 6/condition). Among them, HSP90A was significantly more abundant in fertile than in subfertile males. In co-incubation experiments (n = 3/condition), extracellular vesicles enriched seminal fractions of fertile males showed a higher capacity to be incorporated into fertile than into subfertile sperm. Sperm viability and motility were impacted by the presence of extracellular vesicles from fertile males. In conclusion, we successfully demonstrated the presence of extracellular vesicles in chicken seminal plasma, with differential size, protein markers and putative incorporation capacity according to male fertility status.

scholarly journals Urinary Bisphenol A, F and S Levels and Semen Quality in Young Adult Danish Men

2021 ◽  
Vol 18 (4) ◽  
pp. 1742
Author(s):  
Thea Emily Benson ◽  
Anne Gaml-Sørensen ◽  
Andreas Ernst ◽  
Nis Brix ◽  
Karin Sørig Hougaard ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Male Fertility ◽  
Parental Education ◽  
Negative Binomial ◽  
Semen Quality ◽  
Sperm Count ◽  
Smoking Status ◽  
Sperm Concentration ◽  
First Trimester ◽  
Negative Binomial Regression Model

Bisphenol A (BPA) is considered an endocrine disruptor and has been associated with deleterious effects on spermatogenesis and male fertility. Bisphenol F (BPF) and S (BPS) are structurally similar to BPA, but knowledge of their effects on male fertility remains limited. In this cross–sectional study, we investigated the associations between exposure to BPA, BPF, and BPS and semen quality in 556 men 18–20 years of age from the Fetal Programming of Semen Quality (FEPOS) cohort. A urine sample was collected from each participant for determination of BPA, BPF, and BPS concentrations while a semen sample was collected to determine ejaculate volume, sperm concentration, total sperm count, sperm motility, and sperm morphology. Associations between urinary bisphenol levels (continuous and quartile–divided) and semen characteristics were estimated using a negative binomial regression model adjusting for urine creatinine concentration, alcohol intake, smoking status, body mass index (BMI), fever, sexual abstinence time, maternal pre–pregnancy BMI, and first trimester smoking, and highest parental education during first trimester. We found no associations between urinary bisphenol of semen quality in a sample of young men from the general Danish population.

scholarly journals Macro- and microelements in serum and seminal plasma as biomarkers for bull sperm cryotolerance

2021 ◽  
Vol 63 (1) ◽  
Author(s):  
Maja Zakošek Pipan ◽  
Petra Zrimšek ◽  
Breda Jakovac Strajn ◽  
Katarina Pavšič Vrtač ◽  
Tanja Knific ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Semen Quality ◽  
Sperm Quality ◽  
Membrane Integrity ◽  
Quality Characteristics ◽  
Freezing And Thawing ◽  
Additional Tool ◽  
Bull Semen ◽  
Bull Sperm ◽  
Fresh Semen

ABSTRACT Background Wide variation in fertility rates is observed when using frozen bull semen, even when the bulls have met quality standards for semen production. Therefore, a simple and reliable test to assess the freezing potential of bull semen based on the analysis of fresh semen or blood would be of great value. Attention is now turning to assessment of seminal plasma components such as proteins and elements. In the present study, the concentrations of macro- and microelements in fresh bull semen plasma and in serum and their correlation with quality characteristics of fresh semen and with semen quality after freezing and thawing were determined. Ejaculates were collected from 30 mature bulls, and semen volume, concentration, sperm motility, morphology, tail membrane integrity, plasma membrane permeability and DNA fragmentation were determined on the day of collection and after freezing and thawing. The concentrations of macroelements (Na, Mg, K and Ca) and microelements (Cu, Fe, Zn and Se) were determined in the seminal plasma and serum. The semen samples were classified into satisfactory and unsatisfactory groups according to the fresh semen quality. Results Zinc and Se levels measured in serum were associated with almost all fresh and frozen-thawed semen quality characteristics, while Fe levels were associated only with acrosomal defects in fresh semen. Zinc and Fe levels in fresh seminal plasma were associated with various quality characteristics of fresh and frozen-thawed semen, while Se level in fresh seminal plasma was not associated with any of the semen quality characteristics. Conclusions Microelements were shown to be useful as biomarkers involved in the analysis of bull sperm quality and could be used as an additional tool to predict bull semen quality after freezing and thawing. Our results confirm that the analysis of Zn and Se levels in serum and Zn, Cu and Fe levels in fresh seminal plasma can provide information to discriminate between bull semen samples with spermatozoa with high or low cryotolerance.

Neonicotinoid insecticide metabolites in the seminal plasma: Associations with semen quality

2021 ◽  
pp. 151407
Author(s):  
Aizhen Wang ◽  
Yanjian Wan ◽  
Lixiao Zhou ◽  
Wei Xia ◽  
Yinsheng Guo ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Semen Quality ◽  
Neonicotinoid Insecticide

Seasonal variations in hormones and enzymes of seminal plasma and its relationship with semen quality in crossbred cattle bulls

2018 ◽  
Vol 51 (4) ◽  
pp. 633-643
Author(s):  
Nitika S. Jalmeria ◽  
Sunil Panth ◽  
Sujata Pandita ◽  
A.K. Roy ◽  
Manju Ashutosh ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Seasonal Variations ◽  
Semen Quality ◽  
Crossbred Cattle
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