Gelatinases and their tissue inhibitors are associated with oxidative stress: a potential set of markers connected with male infertility

2016 ◽  
Vol 28 (7) ◽  
pp. 1029 ◽  
Author(s):  
Ewa M. Kratz ◽  
Anna Kałuża ◽  
Mirosława Ferens-Sieczkowska ◽  
Beata Olejnik ◽  
Renata Fiutek ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Seminal Plasma ◽  
Artificial Insemination ◽  
Male Fertility ◽  
Semen Quality ◽  
Reproductive Potential ◽  
Advanced Oxidation Protein Products ◽  
Tissue Inhibitors ◽  
Total Antioxidant ◽  
Infertile Patients

The expression and activity of matrix metalloproteinases (MMPs) may be regulated by oxidative stress in various pathophysiological processes; therefore, the aim of the present study was to analyse the associations between the expression of the gelatinases MMP-9 and MMP-2 and their tissue inhibitors TIMP-1, TIMP-2 and levels of total antioxidant capacity (TAC) and advanced oxidation protein products (AOPP) in seminal plasma prepared for artificial insemination. Levels of MMPs and TIMPs were evaluated using ELISA, whereas TAC and AOPP in the seminal plasma of 131 childless men and 38 fertile volunteers were determined spectrophotometrically. Seminal MMP-9 expression was higher in childless men than in fertile subjects, whereas there was no significant differences in MMP-2 expression between the analysed seminal groups. TIMP-1 and TIMP-2 expression was similar in all groups. However, TAC expression was significantly higher in infertile normozoospermic and oligozoospermic men and AOPP expression was higher in astheno-, oligo- and normozoospermic infertile patients than in fertile men. High AOPP, together with an increased MMP-9 : TIMP-1 ratio alters the oxidative–antioxidative balance of the ejaculate, thereby reducing male fertility, and therefore these parameters may serve as additional diagnostic markers of semen quality and male reproductive potential.

scholarly journals Spermatozoal Fractalkine Signaling Pathway Is Upregulated in Subclinical Varicocele Patients with Normal Seminogram and Low-Level Leucospermia

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Salwa M. Abo El-khair ◽  
Mohammad A. Gaballah ◽  
Mamdouh M. Abdel-Gawad ◽  
Sherif Refaat M. Ismail ◽  
Ayman Z. Elsamanoudy
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Inflammatory Response ◽  
Signaling Pathway ◽  
Dna Fragmentation ◽  
Seminal Plasma ◽  
Semen Quality ◽  
Low Level ◽  
Total Antioxidant ◽  
Receptor Cx3cr1

Background. Fractalkine is produced in seminal plasma in small amounts and correlates with sperm motility. Purpose. To investigate the possible effect of low-level leucospermia on spermatozoa oxidative stress and sDNA fragmentation in patients with subclinical varicocele and apparently normal seminogram, and also to study the role of spermatozoal fractalkine and its receptor (CX3CR1) gene expression as a marker of spermatozoa inflammatory response. Methods. This study included 80 patients with subclinical varicocele (45 fertile and 35 infertile) and 45 age-matched fertile volunteers. In semen samples, fractalkine and CX3CR1 gene expression were investigated by qRT-PCR. Moreover, seminal plasma malondialdehyde (MDA) and total antioxidant capacity (TAC) were measured. Results. There are significant decrease in semen quality and significant increase in seminal leucocytes count in subclinical varicocele. Our results show a significant increase in MDA and TAC levels, DNA fragmentation, and expression levels of fractalkine and its receptor (CX3CR1) in subclinical varicocele groups. Conclusion. Subclinical varicocele induces seminal and spermatozoal subclinical inflammatory response in the form of low-level leucospermia and increased mRNA expression of the fractalkine signaling pathway, leading to increased spermatozoal ROS production, oxidative stress, and DNA fragmentation. These could cooperate in the pathogenesis of delayed fertility in males with subclinical varicocele.

scholarly journals Assessment of the Impact of Oxidative Stress on Frozen Seminal Plasma in Fertile and Infertile Men by Examining the Total Antioxidant Capacity

2014 ◽  
Vol 7 (1) ◽  
pp. 25-30
Author(s):  
Violeta S. Rilcheva ◽  
Nina P. Ayvazova ◽  
Danail I. Martinov ◽  
Cvetomir I. Ivanov ◽  
Emiliana I. Konova
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Seminal Plasma ◽  
Total Antioxidant Capacity ◽  
Dna Integrity ◽  
Integrity Test ◽  
Infertile Men ◽  
Total Antioxidant ◽  
The Impact ◽  
Infertile Patients

AbstractThe aim of the study was to explore the impact of oxidative stress on frozen seminal plasma in fertile and infertile men by examining the total antioxidant capacity. Patients: Infertile patients from male infertility clinic with various diagnoses and fertile men. Design: Seminal plasma from proven fertile men [n=50] and infertile patients [n=50] were examined for total antioxidant capacity (TAC) level, semen parameters such as morphology, motility and concentration, and DNA integrity test. Interventions: Seminal plasma TAC measurement by luminometric assay using the TAC assay kit, semen analysis parameters, DNA integrity test. Fertile men showed higher TAC values (median and SD): 1201µM (SD±548), as compared with the infertile patients: 831μM (SD±343). The result from sperm morphology of fertile patients showed a mean percentage of 4.8 % (SD±1.68) whereas the percentage in the infertile group was 2.68% (SD ±1.68). The same group of samples, analyzed for DNA damage showed a mean of DFI 10.38% (SD±5.17%) in fertile men and a mean of DFI 17.22% (SD±7.22%) in infertile men. Total antioxidant capacity of the seminal plasma as measured by the luminоmetric assay is a reliable and simple test for diagnosing and management of male infertility.

scholarly journals Antioxidants and Polyphenols: Concentrations and Relation to Male Infertility and Treatment Success

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Tali Silberstein ◽  
Iris Har-Vardi ◽  
Avi Harlev ◽  
Michael Friger ◽  
Batel Hamou ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Male Fertility ◽  
Sperm Quality ◽  
Treatment Success ◽  
Sperm Concentration ◽  
Infertility Treatment ◽  
Fertility Treatment ◽  
Fertilization Rates ◽  
Total Antioxidant ◽  
Antioxidant Concentration

Oxidative stress is induced by reactive oxygen substances (ROS) that are known to affect male fertility. The aims of this study were to prospectively investigate and characterize total antioxidant and specifically polyphenols concentrations and their relations to sperm quality and fertility treatment success. During their infertility treatment, sixty-seven males were prospectively recruited to this study. After separation of the sperm from the semen sample, the semen fluid samples antioxidants and polyphenols concentrations were determined. Antioxidant concentration was significantly associated with sperm concentration and total motile count. Antioxidants concentration in the group of male with sperm concentration ≥ 15 × 106was significantly higher than in the group of male with antioxidants concentration < 15 × 106(830.3 ± 350 μM and 268.3 ± 220 μM, resp.,p<0.001). Polyphenols concentration did not differ between the groups of sperm concentration above and below 15 × 106(178.7 ± 121 μM and 161.7 ± 61 μM, resp.,p-NS). No difference was found between fertilization rates and antioxidants or polyphenols concentrations. This is the first study that reports on polyphenols concentration within semen fluid. More studies are needed in order to investigate polyphenols role in male fertility.

scholarly journals Age-related differences of semen quality, seminal plasma, and spermatozoa antioxidative and oxidative stress variables in bulls during cold and warm periods of the year

animal ◽  
2018 ◽  
Vol 12 (3) ◽  
pp. 559-568 ◽  
Author(s):  
S. Vince ◽  
I. Žura Žaja ◽  
M. Samardžija ◽  
I. Majić Balić ◽  
M. Vilić ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Seminal Plasma ◽  
Semen Quality ◽  
Age Related ◽  
And Oxidative Stress

scholarly journals Impact of oxidative stress on semen parameters in normozoospermic infertile men: a case–control study

2020 ◽  
Vol 26 (1) ◽  
Author(s):  
Ayad Palani ◽  
Ahmed Alahmar
Keyword(s):  
Oxidative Stress ◽  
Uric Acid ◽  
Male Infertility ◽  
Seminal Plasma ◽  
Case Control Study ◽  
Case Control ◽  
Semen Parameters ◽  
Infertile Men ◽  
Total Antioxidant ◽  
Control Study

Abstract Background Oxidative stress has been implicated in male infertility through decrease in sperm quality. However, men with normal semen parameters (normozoospermia) may be unable to fertilize their partners even when they have normal sperm function. Thus, they would be considered infertile. The purpose of this study was to investigate the role of oxidative stress in the pathogenesis of unexplained male infertility. Methods In this case–control study, infertile men with normozoospermia (n = 46) and fertile control group (n = 21) underwent seminal fluid analyses according to WHO 2010 criteria. Serum and seminal plasma levels of total antioxidant capacity (TAC), glutathione, malondialdehyde, uric acid and albumin were also measured using colorimetric methods. Results The level of total antioxidant capacity in both serum and seminal plasma was significantly lower in normozoospermic infertile men in comparison with fertile group (p < 0.0001). However, no significant differences were observed in serum and seminal plasma levels of glutathione, uric acid, albumin and malondialdehyde between infertile and fertile groups. Conclusion Low TAC level induces oxidative stress and consequently causes sperm dysfunction and male infertility. Estimation of TAC can be a useful tool in the diagnosis of male infertility. Antioxidant supplementation should be considered in the treatment of oxidative stress-induced male infertility.

scholarly journals Elevated advanced oxidation protein products levels in patients with liver cirrhosis.

2009 ◽  
Vol 56 (4) ◽  
Author(s):  
Jolanta Zuwała-Jagiełło ◽  
Monika Pazgan-Simon ◽  
Krzysztof Simon ◽  
Maria Warwas
Keyword(s):  
Oxidative Stress ◽  
Liver Cirrhosis ◽  
Advanced Oxidation ◽  
Total Antioxidant Status ◽  
Meld Score ◽  
Stress Index ◽  
Serum Concentrations ◽  
Advanced Oxidation Protein Products ◽  
Decompensated Liver Cirrhosis ◽  
Total Antioxidant

Serum concentrations of advanced oxidation protein products (AOPPs) and glycation end products (AGEs) were assessed with respect to functional compromise of liver, as determined by the Child-Pugh and MELD scores. Patients with decompensated liver cirrhosis (Child-Pugh B and C) exhibited significantly higher serum concentrations of AOPPs than both patients with compensated liver cirrhosis (Child-Pugh A) and controls. The levels of plasma AGEs in all liver cirrhotic patients were higher when compared with those with the controls and this difference was statistically significant. Plasma total antioxidant status of the patients was significantly lower than that of controls. Significant positive correlations between AOPPs level and the MELD score and between the oxidative stress index and the MELD score were found in all patients with liver cirrhosis. Altered AOPPs levels in decompensated patients may influence the potency of oxidative stress and the progression of liver disease.

scholarly journals 269IN VITRO FERTILITY OF BOAR SPERMATOZOA PRESERVED AT 10^°C FOR 22 DAYS

2004 ◽  
Vol 16 (2) ◽  
pp. 255
Author(s):  
H. Funahashi
Keyword(s):  
Oxidative Stress ◽  
Functional Status ◽  
Pregnancy Rate ◽  
Seminal Plasma ◽  
Artificial Insemination ◽  
Fluorescence Assay ◽  
Live Cells ◽  
Sperm Viability ◽  
Boar Spermatozoa

Fertility of boar spermatozoa as determined following artificial insemination seems to be maintained during liquid preservation at 10–15°C for several days, although prolonged liquid preservations reduce the pregnancy rate rapidly. However, it is not clear if spermatozoa can penetrate into oocytes in an IVF system even after a prolonged liquid preservation. Oxidative stress could also be one of the possible detrimental factors in liquid preservation of spermatozoa. In the present study, fertility of liquid-preserved spermatozoa was examined using an IVM-IVF system. Whether cysteine can improve the fertility was also determined. Spermatozoa (from four Berkshires) was resuspended at 1×108 cells mL−1 in Modena solution containing 15% (v/v) boar seminal plasma and 0 or 5mM cysteine after washing 3 times. Sperm suspensions (1mL) were then preserved at 10°C for 22 days following a program for cooling down (to 15°C for 4h, keeping at 15°C for 12h and then to 10°C for 6h). At Days 1, 8, 15 and 22 after the start of preservation, spermatozoa (5×105 cells mL−1) were co-cultured with IVM oocytes in an IVM/IVF system (Funahashi et al., 1997 Biol Reprod 57, 49–53). Viability and functional status of spermatozoa were also examined at Days 8 and 15 of preservation by using LIVE/DEAD sperm viability kit and CTC fluorescence assay. Data (mean±SEM) from 4–6 replicates were analyzed by ANOVA and Fisher’s protected LSD test. When spermatozoa that had been preserved without cysteine (Cys−) were used, penetration rates were not different (P&gt;0.05) from those with cysteine (Cys+) at Day 8 of preservation (91.4±3.4% in Cys− and 99.3±0.7% in Cys+), but lower (P&lt;0.02) at Days 15 and 22 (72.6±13.6% and 33.8±8.4% in Cys−; 94.8±2.1% and 71.1±10.8% in Cys+, respectively). Both viability and proportion of uncapacitated live cells were higher (P&lt;0.05) in Cys+ than Cys− at Days 8 and 15. These results demonstrate that boar spermatozoa can penetrate into oocytes in vitro even after a liquid preservation at 10°C for 22 days and that cysteine can improve the viability and penetrability in vitro of spermatozoa during liquid preservation. Supported by the Ito Foundation.

scholarly journals Effect of dietary supplementation with rocket salad (Eruca sativa) seeds powder on certain seminal plasma traits of Hy – line laying breeder roosters subjected to oxidative stress induced by hydrogen peroxide

2012 ◽  
Vol 36 (0A) ◽  
pp. 62-69
Author(s):  
Hazim J. Al – Daraji
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Seminal Plasma ◽  
Control Diet ◽  
Dietary Supplementation ◽  
Control Group ◽  
Total Antioxidant Activity ◽  
Total Antioxidant

This study was conducted to evaluate the effect of adding different levels of rocket salad seeds powder to the diet on seminal plasma traits of roosters subjected to oxidative stress induced by hydrogen peroxide. A total of 60 Hy – line laying breeder roosters 57 weeks old were used in this study. Roosters were randomly distributed into 5 treatments with 3 replicates each. Each replicate constituted of 4 roosters (12 roosters for each treatment). Experimental treatments were as following: T1: Males fed control diet and normal water, T2: Males fed diet supplemented with 3 gm rocket salad powder / kg of diet + 0.25 ml hydrogen peroxide (0.5%) / litter of water, T3: Males fed diet supplemented with 3 gm rocket salad powder / kg of diet + 0.5 ml hydrogen peroxide (0.5%) / litter of water, T4: Males fed diet supplemented with 3 gm rocket salad powder / kg of diet + 1 ml hydrogen peroxide (0.5%) / litter of water, and T5: Males fed control diet and drink tap water supplemented with 1 ml hydrogen peroxide (0.5%) / litter of water. Males were treated with hydrogen peroxide (6%) and rocket salad for 12 weeks starting from 59 week of male ages. Results revealed that treated the roosters with hydrogen peroxide without adding rocket salad powder to the diet of these roosters (T5) resulted in highly significant (p< 0.01) decrease as regards concentrations of phospholipids, cholesterol, glutathione, the activity of superoxide desmutase and catalase, and total antioxidant activity in seminal plasma and highly significant (p< 0.01) increase concerning concentrations of tyrosine and malondialdehyde as compared with control group (T1) and rocket salad powder treatments (T2, T3, T4) after 12 weeks of experiment. However, supplementing diet of roosters with rocket salad powder (T2, T3, T4) resulted in highly significant (p< 0.01) increase with relation to concentrations of phospholipids, cholesterol, glutathione, the activity of superoxide desmutase and catalase, and total antioxidant activity in seminal plasma and highly significant (p< 0.01) decrease respecting concentrations of tyrosine and malondialdehyde as compared with (T5) In conclusion adding rocket salad powder to the diet of roosters had important role in limiting the negative effect of oxidative stress induced by hydrogen peroxide on seminal plasma quality of roosters. Therefore, dietary supplementation with rocket salad powder could be used as one of important tools for improving semen quality of roosters.

Time Dependent Impact of Reactive Oxidants on Seminal Attributes of Murrah Bull during Cryopreservation and Storage

2021 ◽  
Author(s):  
V.R. Upadhyay ◽  
A.K. Roy ◽  
Sujata Pandita ◽  
Raju Kr. Dewry ◽  
Hanuman P. Yadav ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Semen Quality ◽  
Initial Period ◽  
Total Antioxidant ◽  
The Mean ◽  
Ionic Imbalance ◽  
Reactive Oxidants ◽  
And Storage ◽  
Artificial Vagina ◽  
Neat Semen

Background: Cryopreservation is an invaluable technique yet it is also known to be detrimental to sperm function and fertility due to cryo-injury and concomitant generation of reactive oxidants. During laboratory manipulation for the cryopreservation and freeze-thaw process, spermatozoa undergo osmotic stress, ionic imbalance, metabolic decoupling, membrane phase transition, destabilization of the cytoskeleton and antioxidant depletion which communally hampers the semen quality.Methods: With the aim of determining implications of cryopreservation and storage, semen samples were collected by artificial vagina technique from 12 Murrah bulls and subsequently examined at 0 hour (before cryopreservation) and at 24 hour, 1 month and 2 month of storage for various seminal attributes. Simultaneously seminal plasma was separated and preserved at -20oC till the analysis of biochemical indicators of semen quality viz., nitric oxide (NO), total antioxidant quantity (TAC) and lipid peroxidation status (TBARS). Result: A sharp reduction (p less than 0.01) in the semen quality was observed only at 24 h after cryopreservation except for viability. Significant reduction (p less than 0.05) in viable counts was observed up to 1 month interval. The capacitated sperm percentage was greater (p less than 0.01) in the cryopreserved semen as compared to fresh ejaculate. The mean ± SE levels of NO (μmol/L), TAC and TBARS (Units/ml) was 2.31±0.27, 0.73±0.04 and 1.11±0.16 respectively in seminal plasma of neat semen stored at -20oC, while the values in the extended seminal plasma after cryopreservation was 2.37±0.31, 0.44±0.03 and 0.65±0.03 respectively. So it can be inferred that most of the damage encountered by spermatozoa is during the initial period of freezing, but the damage associated by various stressors cannot be ignored.

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