scholarly journals Globodera rostochiensis (Woll.) Behrens (Tylenchida, Heteroderidae), the only potato cyst nematode species found in Finland

1992 ◽  
Vol 1 (5) ◽  
pp. 519-525
Author(s):  
Jari Heikkilä ◽  
Kari Tiilikkala
Keyword(s):  
Isoelectric Focusing ◽  
Nematode Species ◽  
Cyst Nematode ◽  
Soil Samples ◽  
Potato Cyst Nematode ◽  
Potato Seed ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Production Area ◽  
Dimensional Electrophoresis

About 10000 soil samples, 519 thereof infected with potato cyst nematode (PCN), were studied during 1984-1988. Cysts from infected samples were tested by isoelectric focusing to identify PCN species. All the infected samples were also tested with H1-resistant (Satuma) and susceptible (Bintje) potato cultivars to separate resistance breaking populations. Cysts from the roots of Satuma were tested by two-dimensional electrophoresis. The potato seed production area in Finland was found to be free of PCN of any kind. In other parts of Finland all tested samples revealed G. rostochiensis banding pattern, but no G. pallida was found. Except for the most common pathotype Ro1-Ro4, we only found Ro2.

Two-dimensional electrophoresis of proteins with a different approach to isoelectric focusing

The Analyst ◽  
1994 ◽  
Vol 119 (6) ◽  
pp. 1341-1344 ◽  
Author(s):  
S. M. Saqlan Naqvi ◽  
V. Cengiz Özalp ◽  
H. Avni Öktem ◽  
Meral Yücel
Keyword(s):  
Isoelectric Focusing ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis ◽  
Electrophoresis Of Proteins

scholarly journals Molecular and morphological characterization of Globodera rostochiensis (Wollenweber, 1923) Skarbilovich, 1959 from Egypt

2021 ◽  
Vol 13 (4) ◽  
pp. 11083
Author(s):  
Sanaa A. HAROON ◽  
Zafar HANDOO ◽  
Mihail KANTOR ◽  
Andrea SKANTAR ◽  
Maria HULT
Keyword(s):  
Solanum Tuberosum ◽  
Nematode Species ◽  
Morphological Characterization ◽  
Globodera Rostochiensis ◽  
Cyst Nematode ◽  
Soil Samples ◽  
Potato Cyst Nematode ◽  
Cultivated Land ◽  
Zigzag Pattern

The golden potato cyst nematode, Globodera rostochiensis (Wollenweber, 1923) Skarbilovich (1959) is a damaging soilborne quarantine pest of Solanum tuberosum (potato) and other solanaceous crops worldwide. In spring of 2021 a survey was conducted in area of Abo El Matamer, Bahera governorates in Egypt. Soil samples were taken in zigzag pattern throughout 65 acres of potato cultivated land and processed in Nematology lab, Fayoum University, Egypt. In June 2021, two hundred soil samples were collected from nearby areas to evaluate the distribution of this potato cyst nematode in other cultivated land located in area of first infection but fortunately the golden potato cyst nematode was not detected from neighboring locations. The nematode species was identified by both morphological and molecular means as Globodera rostochiensis. To our knowledge this is the first molecular and morphological characterization of G. rostochiensis from Egypt.

Characterisation of potato cyst nematode populations from Portugal

Nematology ◽  
2004 ◽  
Vol 6 (1) ◽  
pp. 55-58 ◽  
Author(s):  
Maria José Da Cunha ◽  
Isabel Luci Da Conceição ◽  
Isabel Maria De O. Abrantes ◽  
Ken Evans ◽  
M. Susana De A. Santos
Keyword(s):  
Isoelectric Focusing ◽  
Specific Protein ◽  
Globodera Rostochiensis ◽  
Cyst Nematode ◽  
Soil Samples ◽  
Potato Cyst Nematode ◽  
Direct Sampling ◽  
Species Specific ◽  
The University

Abstract Populations of potato cyst nematode (PCN) were assembled from all of the potato growing regions of Portugal, either by direct sampling of fields or from cyst samples sent to the University of Coimbra for identification. When 30 or more cysts were available, isoelectric focusing of species-specific protein bands was used to identify which PCN species were present. PCN was present in 35% of a set of 226 soil samples. PCN was also identified in a larger set of 423 populations. Of the 423 populations, 83% were pure Globodera rostochiensis, 8% were pure G. pallida and 9% were mixtures of the two. PCN was found in all of the main potato growing areas but G. pallida was not detected in Coimbra, Setúbal or Viana do Castelo districts.

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