Two-dimensional electrophoresis of proteins with a different approach to isoelectric focusing

The Analyst ◽  
1994 ◽  
Vol 119 (6) ◽  
pp. 1341-1344 ◽  
Author(s):  
S. M. Saqlan Naqvi ◽  
V. Cengiz Özalp ◽  
H. Avni Öktem ◽  
Meral Yücel
Keyword(s):  
Isoelectric Focusing ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis ◽  
Electrophoresis Of Proteins

Analytical electrofocusing and two-dimensional electrophoresis of proteins extracted from the mycelia of aggressive and nonaggressive strains of Ophiostoma ulmi

1986 ◽  
Vol 64 (9) ◽  
pp. 2073-2081 ◽  
Author(s):  
Robert S. Jeng
Keyword(s):  
Dutch Elm Disease ◽  
Two Dimensional ◽  
Polyacrylamide Gels ◽  
Ophiostoma Ulmi ◽  
Two Dimensional Electrophoresis ◽  
Coomassie Blue ◽  
Isoelectric Points ◽  
Additional Protein ◽  
Dimensional Electrophoresis ◽  
Electrophoresis Of Proteins

Soluble mycelial proteins from Ophiostoma ulmi (Buism.) Nannf., the causal agent of Dutch elm disease, were separated by analytical electrofocusing and two-dimensional electrophoresis in polyacrylamide gels. Results showed the aggressive and nonaggressive strains of this pathogen each had about 60 Coomassie blue stained bands having isoelectric points from 3 to 7. Both strains of this fungus had their own characteristic electrofocusing patterns. Nonaggressive isolate S116, for example, lacked two protein bands, one near the anode and one near the cathode, but it had five additional protein bands distributed from pH 4 to 6. Two-dimensional electrophoresis of total soluble proteins depicted that there were 36 proteins found to be specific for the nonaggressive isolate S116 and 12 proteins for the aggressive isolate RR2.

scholarly journals Globodera rostochiensis (Woll.) Behrens (Tylenchida, Heteroderidae), the only potato cyst nematode species found in Finland

1992 ◽  
Vol 1 (5) ◽  
pp. 519-525
Author(s):  
Jari Heikkilä ◽  
Kari Tiilikkala
Keyword(s):  
Isoelectric Focusing ◽  
Nematode Species ◽  
Cyst Nematode ◽  
Soil Samples ◽  
Potato Cyst Nematode ◽  
Potato Seed ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Production Area ◽  
Dimensional Electrophoresis

About 10000 soil samples, 519 thereof infected with potato cyst nematode (PCN), were studied during 1984-1988. Cysts from infected samples were tested by isoelectric focusing to identify PCN species. All the infected samples were also tested with H1-resistant (Satuma) and susceptible (Bintje) potato cultivars to separate resistance breaking populations. Cysts from the roots of Satuma were tested by two-dimensional electrophoresis. The potato seed production area in Finland was found to be free of PCN of any kind. In other parts of Finland all tested samples revealed G. rostochiensis banding pattern, but no G. pallida was found. Except for the most common pathotype Ro1-Ro4, we only found Ro2.

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