scholarly journals Application of Ectomycorrhiza (Scleroderma sp.) in Mangium (Acacia Mangium Willd.) using Artisanal Small-scale Gold Mining(ASGM) Tailings

2020 ◽  
Vol 8 (1) ◽  
pp. 85
Author(s):  
Ulfa Luthfiana ◽  
Melya Riniarti ◽  
Afif Bintoro

Artisanal small-scale gold mining tailings frequently used hazardous toxic materials that could contaminate land, rivers, and lakes which harmful to the environment and health. Phytoremediation is one way to overcome the mining waste. Mangium (Acacia mangium Willd.) is one of plant that often used for mining land phytoremediation. However, it was difficult for mangium to be able to survive on land with such extreme conditions, therefore additional input is needed. Using ectomycorrhiza fungi could be an alternative. This study aimed to measure the adaptation of mangium in gold mining tailings and analyze the function of ectomycorrhiza (Scleroderma sp.) in the growth of mangium. The experimental design used a completely randomized design with six growth media as treatments with five replications. The growth media used were: (1) topsoil with mycorrhiza, (2) topsoil without mycorrhiza, (3) topsoil + tailings with mycorrhiza, (4) soil + tailings without mycorrhiza,(5) tailings with mycorrhiza, and (6) tailings without mycorrhiza. Data were analyzed statistically using analysis of variance followed with a Least Significant Difference test. The results showed that the survival rate of mangium life in tailing media with mycorrhizae was fairly high with a percentage of life reaching 60% even though the growth was less when compared to topsoil media with mycorrhiza that reached 100%. The highest growth rate was achieved by mangium in topsoil media without tailings and mycorrhiza (control). This research showed that ectomycorrhiza did not have any significant effects on mangium growth in gold tailing.Keywords: Acacia mangium, ectomycoriza, gold tailings, phytoremediation, Scleroderma sp.   

2019 ◽  
Vol 7 (3) ◽  
pp. 359
Author(s):  
Beny Kurniawan ◽  
Duryat . ◽  
Melya Riniarti ◽  
Slamet Budi Yuwono

Contamination of mercury (Hg) in illegal gold mining waste could be reduced by the plant. The study aimed to examine the adaptability of mahogany (Swietenia macrophylla) seedling in the growing media of tailings contaminated by mercury. Tailings were taken from the gold refining industry located in Bunut Village, Padang Cermin Sub-district, Lampung Province. The study was designed in a completely randomized design for 5 treatments and 4 replications used combination of topsoil : tailings in a ratio of 100% : 0%; 75% : 25%; 50% : 50%; 25% : 75%; and 0% : 100%. Data were processed by analysis of variance at the 0,05 significance level followed by the Least Significant Difference test. The results showed that mahogany seedling had the adaptability to the media with a maximum tailings concentration of 75%. The higher concentrations of tailings tended to decrease the percentage of life, increment of diameter, increment of the number of leaves, leaf area, the increment of root length, and biomass of mahogany. The higher concentrations of tailings resulted in a better plant height increment compared to the lower concentrations, however, all concentrations of tailings in the planting medium reduced the height of the mahogany seedling.Keywords: Adaptability, mahogany, mercury, tailing


2020 ◽  
Vol 4 (1) ◽  
pp. 58
Author(s):  
Rizal Adi Saputra ◽  
Meyla Riniarti

One of the strategies in rehabilitation of gold mining tailings is used pioneer trees. Pericopsis mooniana is one of pioneer types which capable to adapt to marginal and unproductivel lands. The objective of this research were to find out the growth and physical quality of P. mooniana seedling on artisanal gold mining tailings with the addition of compost elephant dung and humic acid. Growth observation and physical quality assessment were done to P. mooniana seedlings aged 3 months and 10 days, placed on polybag (15/7.5 cm x 20 cm). The experimental design used a completely randomized design with six growth media as treatments and five replications, (1) topsoil 100%, (2) talings 100%, (3) tailings 50% + elephant dungs 50%, (4) tailings 50% + elephant dungs 50% + humic acid (2,000 ppm), (5) tailings 75% + elephant dungs 25%,(6) tailings 75% + elephant dungs 25% + (humic acid (2,000 ppm).  Data were analyzed statistically using analysis of variance followed with a Least Significant Difference test. The stages of the research were: seedling activity, growth observations and physical quality assessment seedlings. The parameter observed were percentage of life, increment height and diameter, number of leaf, leaf area, number of nodules, total dry weight, robustness, ratio of leaves and roots, and seed quality index. The result showed that P. mooniana seedlings were able to grow and adapt to artisanal gold mining tailings with a seedlings quality indexs value of more than (0.09), but the addition of ameliorant did not have any impact yet.


2019 ◽  
Vol 15 (2) ◽  
pp. 105-114
Author(s):  
GUSTI MARLINA ◽  
MARLINDA MARLINDA ◽  
HENI ROSNETI

The research objective was determine the effect of various growth media and growmore fertilizer on the acclimatization of Dendrobium orchids. study used a Completely Randomized Design (CRD) Factorial consisting of 2 factors, the first is (M) of growing media which consists of 3 levels, namely: M1 (medium fern root), M2 (wood charcoal medium), M3 (coir media coconut) and the second is (G) growmore fertilizer which consists of 4 levels, namely: G0 (without Growmore fertilizer), G1 (Growmore fertilizer 2 g / liter), G2 (fertilizer Growmore 4 g / liter), G3 (Growmore fertilizer 6 g / liter). Determine the growth of orchid plants, observations were made with the following parameters Growth Percentage (%), Plant Height (cm), Number of leaves (strands), and Number of tillers (stems). Data obtained were analyzed statistically, then continued with the Honestly Significant Difference Test (BNJ) at the level of 5%. The results showed that the single treatment of the use of growing media did not have a significant effect on the parameters of observation of growth percentage (%), plant height (cm), number of leaves (strands), and number of tillers (stems). The growth of growmore fertilizer also did not show a significant effect on the parameters of observation of growth percentage (%), plant height (cm), number of leaves (strands), and number of tillers (stems). Interaction of the use of various growth media and growmore fertilizer did not show a significant effect on the percentage of growth (%), plant height (cm), number of leaves (strands), and number of tillers (stems).


Author(s):  
Endah Prayekti ◽  
Suliati Suliati ◽  
Dwi Agustin Wulandari

Escherichia coli is the bacteria that can cause diarrhea in humans and often used as a parameter of stool environmental pollution. Culture of E. coli from the sample often requires Mac Conkey as commercial media which is able to distinguish it from other bacteria in the Enterobacteriaceae group. Commercial media such as Mac Conkey certainly has a price that is quite expensive because of its ability as a growth medium for Enterobacteriaceae. Therefore, in the study tested natural ingredients that can be used for growth media, such as coconut water. The purpose of this study was to compare the ability of Mac Conkey media and coconut water to support the growth of E. coli. This research is an experimental study with a completely randomized design. The concentration of coconut water tested was 0%, 20%, 40%, 60%, 80%, and 100%. The results showed that at the concentration of coconut water 20% to 60% the number of E. coli colonies on coconut water media was slightly below the Mac Conkey Agar media, while in coconut water a concentration of 80% showed a greater number of colonies than Mac Conkey. The Mann Whitney test showed a significant difference between the number of colonies on 80% coconut water media and Mac Conkey Agar, which was equal to 0.004 (p < 0.05). Based on these results, coconut water has the potential to be used as a growth medium for E. coli.


2021 ◽  
Vol 9 (1) ◽  
pp. 3219-3225
Author(s):  
Jorge Santiago Garate Quispe ◽  
Rosa Ponce de Leon ◽  
Marx Herrera Machaca ◽  
Edgar Julian Laime ◽  
Carlos Nieto Ramos

In the Peruvian Amazon, large area of primary forest have been deforested by Artisanal and small-scale gold mining (ASGM). Vetiveria zizanioides (Poaceae) is considered an excellent plant for the ecological restoration of degraded lands. The present study aimed to analyze the growth and survivorship of V. zizanioides in degraded soils by gold-mining in the Peruvian Amazon (Madre de Dios). The experiment was conducted under greenhouse conditions, and it followed a randomized complete block design with four treatments (substrates). The substrates were sand (mining), sand+pebbles (mining), forest soil, and amended soil. We evaluated the survivorship, shoot, tiller, and biomass production of V. zizanioides for two months. Univariate analysis of variance was used to detect differences among treatments. Fifteen days after experiment establishment, V. zizanioides survivorship was significantly higher in ASGM substrate 2 (sand+pebbles) than in other substrates, following the order of ASGM substrate 1 (sand) > forest soil > amended soil. However, at the end of the experiment, we did not find a significant difference on survivorship in subsequent assessments, and the total biomass per plant was lower in mining substrates than in non-mining substrates. The total biomass was significantly higher in amended soil than in other substrates, with yields between 1.7 and 3.6 times more biomass in amended substrate than in mine substrates. The soil substrate type significantly affected the survivorship, number of shoots, and biomass of V. zizanioides. The research results can provide a reference for remediation of degraded soils by gold-mining in the Peruvian Amazon.


2020 ◽  
Vol 16 (1) ◽  
pp. 49-62
Author(s):  
محي الدين جمعة عبد الله

The effect of adding different sugar concentrations of five treatments (T1, T2, T3, T4, T5) on rose growth CV Sarah in vitro was carried out in the Sudan University Laboratories. Data were collected for plant height, number of branches, number of leaves, and fresh weight. Statistical analysis was conducted using a completely randomized design, to find the least significant difference to compare between treatment means for MS (Murashige and Skoog) medium. Data analysis showed that the application of sugar at concentrations of 15, 30, and 45gms gave the highest means for the different growth parameters and fresh weight at a significant level, whereas the addition of sugar at 60gms concentration and sugar-free gave no significant increases. This showed clearly that addition of sugar was essential for plant growth, greater concentrations gaviz 60gm negative results, Addition of sugar at the above-mentioned concentration from 15mg,45gm to parameters studied the growth media in vitro gave a positive increase in rose growth.


2020 ◽  
Vol 21 (6) ◽  
pp. 127-136
Author(s):  
Siska Nurfitriani ◽  
Endang Arisoesilaningsih ◽  
Yulia Nuraini ◽  
Eko Handayanto

2018 ◽  
Vol 6 (2) ◽  
pp. 729
Author(s):  
Berta Putri ◽  
Siti Hudaidah ◽  
Widi Indra Kesuma

The purpose of this research was to observe the effect of fermented palm kernel cake as a growth media for Tubifex sp. This research was conducted at the Aquaculture Laboratory, University of Lampung for 50 days. Completely Randomized Design (CDR) with 5 treatments and 3 replicates design is used for this experiment. The treatments are 100% field mud, 25% palm kernel cake :75% field mud, 50% palm kernel cake : 50% field mud, 75% palm kernel cake : 25% field mud, and 100% palm kernel cake. The data were analyzed with analysis of variance (ANOVA) continued with Least Significant Difference (LSD) test. The main parameters measured were Tubifex sp population and biomass as well as water quality parameters: pH, temperature, dissolved oxygen, and ammonia. The results showed that fermented palm kernel cake significantly (p<0,05) increase silk worm biomass and population. The best result of this research was found in 50% fermented palm kernel cake and 50% field mud media producing 111.008 ind/m2 and 750,72 gr/m2­­­­ of Tubifex sp. Water quality during Tubifex sp were measured at 0,26-0,91 mg/l ammonia, pH 6,14-7,11, temperature 25-28°C, and dissolved oxygen 2,9-3,9 mg/l.


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