Evaluation of biocompatibility and efficiency of plasmid DNA delivery by gene-activated hydrogels in vitro

2019 ◽  
Vol XIV (2) ◽  
Author(s):  
I.Y. Bozo ◽  
A.A. Titova ◽  
M.N. Zhuravleva ◽  
A.I. Bilyalov ◽  
M.O. Mavlikeev ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Dna Delivery

scholarly journals Arginine-based cationic liposomes for efficient in vitro plasmid DNA delivery with low cytotoxicity

2013 ◽  
pp. 1361 ◽  
Author(s):  
Shinji Takeoka ◽  
Sarker ◽  
Aoshima ◽  
Hokama ◽  
Inoue ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Dna Delivery ◽  
Cationic Liposomes ◽  
Low Cytotoxicity

scholarly journals Investigation of Plasmid DNA Delivery and Cell Viability Dynamics for Optimal Cell Electrotransfection In Vitro

2020 ◽  
Vol 10 (17) ◽  
pp. 6070
Author(s):  
Sonam Chopra ◽  
Paulius Ruzgys ◽  
Martynas Maciulevičius ◽  
Milda Jakutavičiūtė ◽  
Saulius Šatkauskas
Keyword(s):  
Cell Membrane ◽  
Cell Viability ◽  
High Voltage ◽  
Plasmid Dna ◽  
Transfection Efficiency ◽  
Dna Delivery ◽  
Output Parameter ◽  
Gene Electrotransfer ◽  
Electric Pulses

Electroporation is an effective method for delivering plasmid DNA molecules into cells. The efficiency of gene electrotransfer depends on several factors. To achieve high transfection efficiency while maintaining cell viability is a tedious task in electroporation. Here, we present a combined study in which the dynamics of both evaluation types of transfection efficiency and the cell viability were evaluated in dependence of plasmid concentration as well as at the different number of high voltage (HV) electric pulses. The results of this study reveal a quantitative sigmoidal (R2 > 0.95) dependence of the transfection efficiency and cell viability on the distance between the cell membrane and the nearest plasmid. We propose this distance value as a new, more accurate output parameter that could be used in further optimization studies as a predictor and a measure of electrotransfection efficiency.

In vitro study of plasmid DNA delivery systems for mucosal immunization against TBE

2002 ◽  
Vol 291 ◽  
pp. 172
Author(s):  
E.P. Goncharova ◽  
L.E. Bulychev ◽  
A.A. Sizov ◽  
V.D. Poryvaev ◽  
L.I. Karpenko ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
In Vitro Study ◽  
Dna Delivery ◽  
Delivery Systems ◽  
Vitro Study ◽  
Mucosal Immunization

Multifunctional self-assembled cationic peptide nanostructures efficiently carry plasmid DNA in vitro and exhibit antimicrobial activity with minimal toxicity

2014 ◽  
Vol 2 (30) ◽  
pp. 4848-4861 ◽  
Author(s):  
Santosh Yadav ◽  
Manohar Mahato ◽  
Rajiv Pathak ◽  
Diksha Jha ◽  
Bipul Kumar ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Plasmid Dna ◽  
Cationic Peptide ◽  
Minimal Toxicity ◽  
Amphiphilic Peptide ◽  
Self Assembled ◽  
Multifunctional Properties

An amphiphilic peptide–aminoglycoside (Pep–Neo) conjugate has been synthesized, self-assembled into nanostructures and evaluated for its multifunctional properties.

Efficient Cellular Entry of (r-x-r)-Type Carbamate–Plasmid DNA Complexes and Its Implication for Noninvasive Topical DNA Delivery to Skin

2016 ◽  
Vol 13 (6) ◽  
pp. 1779-1790 ◽  
Author(s):  
Manika Vij ◽  
Poornemaa Natarajan ◽  
Amit K. Yadav ◽  
Kiran M. Patil ◽  
Tanuja Pandey ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Dna Delivery ◽  
Dna Complexes ◽  
Cellular Entry
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