Multifunctional self-assembled cationic peptide nanostructures efficiently carry plasmid DNA in vitro and exhibit antimicrobial activity with minimal toxicity

2014 ◽  
Vol 2 (30) ◽  
pp. 4848-4861 ◽  
Author(s):  
Santosh Yadav ◽  
Manohar Mahato ◽  
Rajiv Pathak ◽  
Diksha Jha ◽  
Bipul Kumar ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Plasmid Dna ◽  
Cationic Peptide ◽  
Minimal Toxicity ◽  
Amphiphilic Peptide ◽  
Self Assembled ◽  
Multifunctional Properties

An amphiphilic peptide–aminoglycoside (Pep–Neo) conjugate has been synthesized, self-assembled into nanostructures and evaluated for its multifunctional properties.

Self-assembled Cationic Peptide Nanoparticles Capable of Inducing Efficient Gene Expression In Vitro

2008 ◽  
Vol 18 (6) ◽  
pp. 943-951 ◽  
Author(s):  
Nikken Wiradharma ◽  
Majad Khan ◽  
Yen Wah Tong ◽  
Shu Wang ◽  
Yi-Yan Yang
Keyword(s):  
Gene Expression ◽  
Cationic Peptide ◽  
Efficient Gene ◽  
Peptide Nanoparticles ◽  
Self Assembled

scholarly journals In Vitro Antimicrobial Activity of MSI-78, a Magainin Analog

1998 ◽  
Vol 42 (5) ◽  
pp. 1213-1216 ◽  
Author(s):  
Peter C. Fuchs ◽  
Arthur L. Barry ◽  
Steven D. Brown
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Bactericidal Activity ◽  
Broad Spectrum ◽  
Vitro Activity ◽  
Topical Agent ◽  
In Vitro Activity ◽  
Cationic Peptide ◽  
In Vitro Antimicrobial Activity

ABSTRACT MSI-78 is a cationic peptide with broad-spectrum antimicrobial activity and is being developed as a topical agent. We compared the in vitro activity of MSI-78 with those of ofloxacin and other antibiotics against fresh clinical isolates. Based on MIC distribution statistics, strains for which the MSI-78 MIC was ≤64 μg/ml were assumed to be susceptible for purposes of this report. Of 411 aerobic isolates tested, 91% were susceptible to MSI-78, compared to 91% for ofloxacin and 92% for ciprofloxacin. Only enterococci consistently required ≥64 μg of MSI-78/ml for inhibition. MSI-78 demonstrated bactericidal activity equivalent to that of ofloxacin. Of 61 anaerobes, 97% were susceptible to MSI-78. Of 10 isolates of Candida albicans, 3 were inhibited by MSI-78 at 24 h. Further studies of this compound appear to be warranted.

Investigation of the antimicrobial activity of a short cationic peptide against promastigote and amastigote forms of Leishmania major (MHRO/IR/75/ER): An in vitro study

2019 ◽  
Vol 196 ◽  
pp. 48-54 ◽  
Author(s):  
Sara khalili ◽  
Elahe Ebrahimzade ◽  
Mehdi Mohebali ◽  
Parviz Shayan ◽  
Samira Mohammadi-Yeganeh ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Leishmania Major ◽  
In Vitro Study ◽  
Vitro Study ◽  
Cationic Peptide

scholarly journals De Novo Design of a pH-triggered Self-assembled β-hairpin Nano Peptide for the Dual Biological Function of Antibacterial and Entrapment

2021 ◽  
Author(s):  
Qiuke Li ◽  
Jinze Li ◽  
Weikang Yu ◽  
Zhihua Wang ◽  
Jiawei Li ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
De Novo ◽  
Ph Regulation ◽  
Membrane Integrity ◽  
Intestinal Flora ◽  
Building Blocks ◽  
Self Assembled ◽  
High Concentrations

Abstract BackgroundAcid-tolerant enteric pathogens could evade small intestinal acid barriers, colonizing and infecting the intestinal tract. Whereas broad-spectrum antibiotics are not the best therapeutic strategy because of the disruption of intestinal flora caused by its indiscriminate antimicrobial activity against beneficial and harmful bacteria. So that is what inspired us to combine pH regulation with nanotechnology to develop a pH-triggered site-targeted antimicrobial peptide with entrapping function. ResultsAccording to the features of amino-acid building blocks and the diagonal cation–π interaction principle, a self-assembled peptide (SAP) was designed, and the results showed that changes in pH conditions could trigger the transformation of the microstructure of the nanopeptide, which has great antimicrobial activity against Escherichia coli, Salmonella typhimurium, Listeria monocytogenes, and Bacillus cereus under acidic conditions by disrupting bacterial membrane integrity, and great biocompatibility in vivo and in vitro and high tolerance. Moreover, SAP at high concentrations showed the entrapment property, which plays an important role in phagocytic clearance in the infection forces.ConclusionsOur study revealed the antibacterial activity of a short β-hairpin forming self-assembled peptide and establishes an innovative design strategy for peptide-based nanomaterials and a new treatment strategy for gastrointestinal bacterial infection.

scholarly journals De novo design of a pH-triggered self-assembled β-hairpin nanopeptide with the dual biological functions for antibacterial and entrapment

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Qiuke Li ◽  
Jinze Li ◽  
Weikang Yu ◽  
Zhihua Wang ◽  
Jiawei Li ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Bacterial Infections ◽  
De Novo ◽  
Ph Regulation ◽  
Membrane Integrity ◽  
Intestinal Flora ◽  
Building Blocks ◽  
Self Assembled

Abstract Background Acid-tolerant enteric pathogens can evade small intestinal acid barriers, colonize and infect the intestinal tract. However, broad-spectrum antibiotics are not the best therapeutic strategy because of the disruption of intestinal flora caused by its indiscriminate antimicrobial activity against beneficial and harmful bacteria. So that is what inspired us to combine pH regulation with nanotechnology to develop a pH-triggered site-targeted antimicrobial peptide with entrapping function. Results A pH-triggered dual biological functional self-assembled peptide (SAP) was designed according to the features of amino-acid building blocks and the diagonal cation–π interaction principle. The results of characterization experiments showed that changes in pH conditions could trigger microstructural transformation of the nanopeptide from nanospheres to nanofibers. The subsequent antibacterial and toxicity experiments determined that SAP had great antimicrobial activity against Escherichia coli, Salmonella typhimurium, Listeria monocytogenes, and Bacillus cereus above 15.6 μg/mL under acidic conditions by disrupting bacterial membrane integrity, excellent biocompatibility in vitro even at 250 μg/mL and high tolerance in physical environment. Moreover, at peptide concentrations greater than 62.5 μg/mL, SAP showed the entrapment property, which played an important role in phagocytic clearance in infection forces. Meanwhile, the in vivo results revealed that SAP possessed excellent therapeutic effect and good biosafety. Conclusions Our study revealed the antibacterial activity of a short β-hairpin forming self-assembled peptide, and established an innovative design strategy for peptide-based nanomaterials and a new treatment strategy for gastrointestinal bacterial infections. Graphic Abstract

scholarly journals A Hybrid Cationic Peptide Composed of Human β-Defensin-1 and Humanized θ-Defensin Sequences Exhibits Salt-Resistant Antimicrobial Activity

2014 ◽  
Vol 59 (1) ◽  
pp. 217-225 ◽  
Author(s):  
Sudar Olli ◽  
Ramakrishnan Nagaraj ◽  
Swapna R. Motukupally
Keyword(s):  
Antimicrobial Activity ◽  
Disulfide Bridge ◽  
Linear Molecule ◽  
Cationic Peptide ◽  
Hybrid Peptide ◽  
Eye Infections ◽  
Gram Positive Bacteria ◽  
New Class ◽  
Circular Molecule

ABSTRACTWe have designed a hybrid peptide by combining sequences of human β-defensin-1 (HBD-1) and θ-defensin, in an attempt to generate a molecule that combines the diversity in structure and biological activity of two different peptides to yield a promising therapeutic candidate. HBD-1 was chosen as it is a natural defensin of humans that is constitutively expressed, but its antibacterial activity is considerably impaired by elevated ionic strength. θ-Defensins are expressed in human bone marrow as a pseudogene and are homologous to rhesus monkey circular minidefensins. Retrocyclins are synthetic human θ-defensins. The cyclic nature of the θ-defensin peptides makes them salt resistant, nonhemolytic, and virtually noncytotoxicin vitro. However, a nonhuman circular molecule developed for clinical use would be less viable than a linear molecule. In this study, we have fused the C-terminal region of HBD-1 to the nonapeptide sequence of a synthetic retrocyclin. Cyclization was achieved by joining the terminal ends of the hybrid peptide by a disulfide bridge. The hybrid peptide with or without the disulfide bridge exhibited enhanced antimicrobial activity against both Gram-negative and Gram-positive bacteria as well as against fungi, including clinical bacterial isolates from eye infections. The peptide retained activity in the presence of NaCl and serum and was nonhemolyticin vitro. Thus, the hybrid peptide generated holds potential as a new class of antibiotics.

scholarly journals In Vitro Activities of Pexiganan and 10 Comparator Antimicrobials against 502 Anaerobic Isolates Recovered from Skin and Skin Structure Infections

2017 ◽  
Vol 61 (12) ◽  
Author(s):  
Ellie J. C. Goldstein ◽  
Diane M. Citron ◽  
Kerin L. Tyrrell ◽  
Eliza S. Leoncio
Keyword(s):  
Antimicrobial Activity ◽  
Clostridium Perfringens ◽  
Adjunctive Therapy ◽  
Propionibacterium Acnes ◽  
Bacteroides Fragilis ◽  
Cationic Peptide ◽  
Gram Positive ◽  
Content Type ◽  
Skin Structure

ABSTRACTPexiganan, a cationic peptide, exhibited a broad range of anti-anaerobic antimicrobial activity. The MIC90s of studied isolates were as follows:Bacteroides fragilis, 16 μg/ml; otherB. fragilisgroup spp., 4 μg/ml;PrevotellaandFusobacteriumspp., 32 μg/ml;Porphyromonasspp., 64 μg/ml;Propionibacterium acnes, 4 μg/ml;Eggerthella lentaandPeptostreptococcus anaerobius, 32 μg/ml; other Gram-positive rods and cocci, 4 μg/ml;Clostridium perfringens, 128 μg/ml; and other clostridia, 256 μg/ml. Pexiganan cream shows potential as adjunctive therapy for skin and skin structure infections (SSSIs) involving anaerobes.

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