Visible lesion threshold modeling of skin laser exposure at 1070-nm

2019 ◽  
Author(s):  
Michael P. DeLisi ◽  
Nicholas J. Gamez ◽  
ElHarith M. Ahmed ◽  
Chad A. Oian ◽  
Benjamin A. Rockwell ◽  
...  
Keyword(s):  
Laser Exposure ◽  
Visible Lesion ◽  
Threshold Modeling

Minimum visible lesion (MVL) thresholds in cynomolgus (Macaca fasicularis) retina resulting from laser exposure at wavelengths of 413-nm, 532-nm and 647-nm

2011 ◽  
Author(s):  
Jeffrey W. Oliver ◽  
Benjamin A. Rockwell ◽  
Semih S. Kumru ◽  
Clifton D. Clark ◽  
Harvey M. Hodnett ◽  
...  
Keyword(s):  
Laser Exposure ◽  
Visible Lesion ◽  
532 Nm

Chronic Laser Exposure Effects on Rabbit Retina

1972 ◽  
Vol 30 ◽  
pp. 54-55
Author(s):  
Burton B. Silver ◽  
Theodore Lawwill
Keyword(s):  
Laser Irradiation ◽  
Laser Light ◽  
Broad Band ◽  
Argon Laser ◽  
Atropine Sulfate ◽  
Ultrastructural Changes ◽  
Laser Exposure ◽  
Rabbit Retina ◽  
Histological Changes ◽  
Threshold Doses

Dutch-belted 1 to 2.5 kg anesthetized rabbits were exposed to either xenon or argon laser light administered in a broad band, designed to cover large areas of the retina. For laser exposure, the pupil was dilated with atropine sulfate 1% and pheny lephrine 10%. All of the laser generated power was within a band centered at 5145.0 Anstroms. Established threshold for 4 hour exposures to laser irradiation are in the order of 25-35 microwatts/cm2. Animals examined for ultrastructural changes received 4 hour threshold doses. These animals exhibited ERG, opthalmascopic, and histological changes consistent with threshold damage.One month following exposure the rabbits were killed with pentobarbitol. The eyes were immediately enucleated and dissected while bathed in 3% phosphate buffered gluteraldehyde.

Fading of the diaminobenzidine reaction product in the confocal scanning laser microscope

1989 ◽  
Vol 47 ◽  
pp. 146-147
Author(s):  
JS Deitch ◽  
KL Smith ◽  
JW Swann ◽  
JN Turner
Keyword(s):  
Pyramidal Neurons ◽  
Light And Electron Microscopy ◽  
Scanning Laser ◽  
Laser Exposure ◽  
Confocal Scanning Laser Microscope ◽  
Fluorescent Markers ◽  
Before And After ◽  
Staining Protocol ◽  
Confocal Scanning ◽  
Confocal Scanning Laser

Neurons labeled with horseradish peroxidase and reacted with diaminobenzidine (DAB) can be imaged using a confocal scanning laser microscope (CSLM) in the reflection mode. In contrast to fluorescent markers, the DAB reaction product is thought to be stable and can be observed by both light and electron microscopy. We have investigated the sensitivity of the DAB reaction product to laser irradiation, and present the spectrophotometric properties of DAB before and after exposure in the CSLM.Pyramidal neurons in slices of rat hippocampus were injected with biocytin (a biotin-lysine complex), fixed overnight in 4% paraformaldehyde, and vibratome sectioned at 75 μm. Biocytin was detected with avidin-HRP (1:200) in 0.5% Triton X-100, incubated in DAB (0.5 mg/ml) with or without 0.04% nickel ammonium sulfate (Ni), dehydrated, and imaged in a Bio Rad MRC-500 CSLM with an argon ion laser (488 and 514 nm). Spectrophotometric measurements of the soma were made on a Zeiss microspectrophotometer, as a function of laser exposure (100-1000 scans) and staining protocol.

scholarly journals The Utility of Rapid On-Site Evaluation during Bronchoscopic Biopsy: A 2-Year Respiratory Endoscopy Central Experience

2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Hansheng Wang ◽  
Na Wei ◽  
Yijun Tang ◽  
Yunyun Wang ◽  
Guoshi Luo ◽  
...  
Keyword(s):  
Lung Cancer ◽  
International Association ◽  
Final Diagnosis ◽  
Needle Aspiration ◽  
European Respiratory Society ◽  
Macroscopic Appearance ◽  
Visible Lesion ◽  
Bronchoscopic Biopsy ◽  
Site Evaluation

Background. Rapid on-site evaluation (ROSE) is commonly used to evaluate the adequacy of biopsy materials in fine-needle aspiration; however, the diagnostic performance of ROSE during fiber optic bronchoscopy (FOB) biopsy under direct vision is rarely reported. Here, we evaluated the role of ROSE during FOB biopsy of visible lesion in trachea or bronchi. Methods. The role of ROSE was prospectively evaluated in consecutive bronchoscopy specimens obtained between January 2016 and January 2018. The agreement and accuracy between ROSE and final histopathological interpretation were assessed. The frequency and possible reasons for discrepancy between ROSE and definitive histopathology results were identified. Histological and cytological classification was performed according to the International Association for the Study of Lung Cancer, the American Thoracic Society, and the European Respiratory Society (IASLC/ATS/ERS) criteria of lung ADCs classification. Results. The study enrolled 651 patients, of which 33 were excluded because of insufficient cells. Final diagnosis of malignancy was achieved in 462 cases (74.8%), whereas 156 cases (25.2%) were nonmalignant. ROSE and pathology were well correlated for the diagnosis of squamous cell carcinoma (SCC) (Kappa = 0.718, p<0.05), adenocarcinoma (AdC) (Kappa = 0.662; p<0.05) and small cell lung cancer (SCLC) (Kappa = 0.955; p<0.05). In 24 cases diagnosed as malignant by ROSE and nonmalignant by pathology, the lesion tissues were surgically excised and re-analyzed, and the 24 cases were finally confirmed as malignant by pathology. Conclusions. ROSE technique allows bronchoscopists to obtain viable and adequate material for the diagnosis of histopathology, and provides them with an onsite preliminary diagnosis especially in cases with inconclusive macroscopic appearance. ROSE and pathology should be used in combination to increase the accuracy of diagnosis.

Photoluminescence of Point Defects in Silicon Dioxide by Femtosecond Laser Exposure

2021 ◽  
Author(s):  
Vincenzo De Michele ◽  
Emmanuel Marin ◽  
Aziz Boukenter ◽  
Marco Cannas ◽  
Sylvain Girard ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Silicon Dioxide ◽  
Point Defects ◽  
Laser Exposure

Multimodale Bronchoskopie in der Rundherddiagnostik: Gewinnbringende Kombinationen ausloten

2021 ◽  
pp. 1-3
Author(s):  
Axel Tobias Kempa
Keyword(s):  
Diagnostic Yield ◽  
Target Lesion ◽  
Endobronchial Ultrasound ◽  
External Diameter ◽  
Pulmonary Lesions ◽  
Diagnostic Bronchoscopy ◽  
Peripheral Lung ◽  
Visible Lesion ◽  
Median Diameter ◽  
Pulmonary Tumors

There are cases of peripheral lung nodules that are difficult to approach despite using ancillary diagnostic devices during multimodal bronchoscopy. The use of ultrathin bronchoscopes has shown superiority over standard thin bronchoscopes. We retrospectively evaluated whether substitution of the thin-bronchoscope by the ultrathin device during multimodal bronchoscopy improves lesion ultrasound visualization and diagnostic yield in patients with difficult-to-approach pulmonary lesions. The study comprised 44 out of 338 patients that underwent multimodal bronchoscopy at Matsusaka Municipal Hospital. The thin-bronchoscope with an external diameter of 4 mm was substituted by the ultrathin-bronchoscope with an external diameter of 3 mm when the radial endobronchial ultrasound showed that the probe position was not within the target lesion. The median diameter of the pulmonary tumors was 17.5 mm (range: 6.0–5.2.0 mm). The endobronchial ultrasound showed the probe’s position adjacent to the lesion in 12 cases and no visible lesion in 32 cases using a thin-bronchoscope. However, the endobronchial ultrasound views changed from adjacent to the lesion to within the lesion in nine cases, from no visible lesion to within the lesion in 17 cases, and from no visible lesion to adjacent to the lesion in nine cases after bronchoscope substitution. After substitution, the diagnostic yield was 80.8% in cases with the radial probe within the target lesion, 72.7% in cases with the probe adjacent to the target lesion, and 0% in cases with no visible lesion. The overall diagnostic yield was 65.9% after bronchoscope substitution. The substitution of the thin bronchoscope by the ultrathin device on a need basis improves the position of the radial endobronchial ultrasound probe and diagnostic yield of pulmonary lesions during multimodal diagnostic bronchoscopy.

scholarly journals Formation of Brightly Luminescent MoS2 Nanoislands from Multilayer Flakes via Plasma Treatment and Laser Exposure

ACS Omega ◽  
2020 ◽  
Vol 5 (32) ◽  
pp. 20543-20547
Author(s):  
Bo Wang ◽  
Sisi Yang ◽  
Yu Wang ◽  
Younghee Kim ◽  
Han Htoon ◽  
...  
Keyword(s):  
Plasma Treatment ◽  
Laser Exposure

TP53 and ATM mRNA expression in skin and skeletal muscle after low-level laser exposure

2017 ◽  
Vol 19 (4) ◽  
pp. 227-231 ◽  
Author(s):  
Luciana Guedes de Almeida ◽  
Luiz Philippe da Silva Sergio ◽  
Flavia de Paoli ◽  
Andre Luiz Mencalha ◽  
Adenilson de Souza da Fonseca
Keyword(s):  
Skeletal Muscle ◽  
Mrna Expression ◽  
Laser Exposure ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser
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