scholarly journals Umbilical Cord Blood and Type 1 Diabetes: A road ahead or dead end?

Diabetes Care ◽  
2009 ◽  
Vol 32 (11) ◽  
pp. 2138-2139 ◽  
Author(s):  
D. Bleich
Keyword(s):  
Type 1 Diabetes ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Dead End

scholarly journals Umbilical Cord Blood DNA Methylation in Children Who Later Develop Type 1 Diabetes

2021 ◽  
Author(s):  
Essi Laajala ◽  
Ubaid Ullah ◽  
Toni Grönroos ◽  
Omid Rasool ◽  
Viivi Halla-aho ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Type 1 Diabetes ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Reduced Representation ◽  
Prediction And Prevention ◽  
Develop Type ◽  
Methylation Patterns

Distinct DNA methylation patterns have recently been observed to precede type 1 diabetes in whole blood collected from young children. Our aim was to determine, whether perinatal DNA methylation could be associated with later progression to type 1 diabetes. Reduced representation bisulfite sequencing (RRBS) analysis was performed on umbilical cord blood samples collected within the Type 1 Diabetes Prediction and Prevention (DIPP) study. Children later diagnosed with type 1 diabetes and/or testing positive for multiple islet autoantibodies (N=43) were compared to control individuals (N=79), who remained autoantibody-negative throughout the DIPP follow-up until 15 years of age. Potential confounding factors related to the pregnancy and the mother were included in the analysis. No differences in the cord blood methylation patterns were observed between these cases and controls.

scholarly journals Autologous umbilical cord blood infusion for type 1 diabetes

2008 ◽  
Vol 36 (6) ◽  
pp. 710-715 ◽  
Author(s):  
Michael J. Haller ◽  
Hilla-Lee Viener ◽  
Clive Wasserfall ◽  
Todd Brusko ◽  
Mark A. Atkinson ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood

scholarly journals Autologous Umbilical Cord Blood Transfusion in Young Children With Type 1 Diabetes Fails to Preserve C-Peptide

Diabetes Care ◽  
2011 ◽  
Vol 34 (12) ◽  
pp. 2567-2569 ◽  
Author(s):  
M. J. Haller ◽  
C. H. Wasserfall ◽  
M. A. Hulme ◽  
M. Cintron ◽  
T. M. Brusko ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Blood Transfusion ◽  
Young Children ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
C Peptide

scholarly journals Autologous Umbilical Cord Blood Transfusion in Very Young Children With Type 1 Diabetes

Diabetes Care ◽  
2009 ◽  
Vol 32 (11) ◽  
pp. 2041-2046 ◽  
Author(s):  
M. J. Haller ◽  
C. H. Wasserfall ◽  
K. M. McGrail ◽  
M. Cintron ◽  
T. M. Brusko ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Blood Transfusion ◽  
Young Children ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Very Young Children

scholarly journals Permutation-based significance analysis reduces the type 1 error rate in bisulfite sequencing data analysis of human umbilical cord blood samples

2021 ◽  
Author(s):  
Essi Laajala ◽  
Viivi Halla-aho ◽  
Toni Grönroos ◽  
Ubaid Ullah ◽  
Mari Vähä-Mäkilä ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Error Control ◽  
Bisulfite Sequencing ◽  
Differential Methylation ◽  
Gestational Weight ◽  
Type 1 Error ◽  
Analysis Workflow

Background: The aim of this study was to detect differential methylation in umbilical cord blood that is associated with maternal and pregnancy-related variables, such as maternal age and gestational weight gain. These have been studied earlier with 450K microarrays but not with bisulfite sequencing. Methods: Reduced representation bisulfite sequencing (RRBS) analysis was performed on 200 umbilical cord blood samples. Altogether 24 clinical and technical covariates were included in a binomial mixed effects model, which was fit separately for each high-coverage CpG site, followed by spatial and multiple testing adjustment of P values. Inflation of spatially adjusted P values was discovered in a permutation analysis, which was then applied for empirical type 1 error control. Results: Empirical type 1 error control decreased the number of findings associated with each covariate to zero or a small fraction of the number that would have been discovered with standard cutoffs. In this collection of samples, some differential methylation was associated with sex, the usage of epidural anesthetic during delivery, 1 minute Apgar points, maternal age and height, gestational weight gain, maternal smoking, and maternal insulin-treated diabetes, but not with the birth weight of the newborn infant, maternal pre-pregnancy BMI, the number of earlier miscarriages, the mode of delivery, labor induction, or the cosine transformed month of birth. Conclusions: The autocorrelation-adjusted Z-test is a convenient tool for detecting differentially methylated regions, but the significance should either be determined empirically or before the spatial adjustment. With appropriate significance thresholds, the detected differentially methylated regions were reproducible across studies, technologies, and statistical models. Our RRBS data analysis workflow is available in https://github.com/EssiLaajala/RRBS_workflow. Keywords: DNA methylation, bisulfite sequencing, RRBS, umbilical cord blood, pregnancy, sex, spatial correlation, type 1 error, differential methylation, analysis workflow

scholarly journals Transduction of Umbilical Cord Blood CD34+ NOD/SCID-Repopulating Cells by Simian Foamy Virus Type 1 (SFV-1) Vector

Virology ◽  
2002 ◽  
Vol 302 (2) ◽  
pp. 229-235 ◽  
Author(s):  
James R. Zucali ◽  
Tina Ciccarone ◽  
Vicky Kelley ◽  
Jeonghae Park ◽  
Calvin M. Johnson ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Virus Type ◽  
Umbilical Cord Blood ◽  
Foamy Virus ◽  
Simian Foamy Virus ◽  
Cord Blood Cd34
Sign in / Sign up

Export Citation Format

Share Document