2289-PUB: Comparison of GalT-KO Porcine Islet Xenograft Survival between Microcapsule against Hypoxia and Microcapsule against Fibrosis in Diabetic Mice Model

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 2289-PUB
Author(s):  
EUN YOUNG LEE ◽  
YEOREE YANG ◽  
YUNJUNG CHO ◽  
SEUNG-HWAN LEE ◽  
JAE-HYOUNG CHO ◽  
...  
Keyword(s):  
Diabetic Mice ◽  
Mice Model ◽  
Porcine Islet

A mitochondria-targeted near-infrared fluorescent probe for imaging viscosity in living cells and a diabetic mice model

2021 ◽  
Author(s):  
Bochao Chen ◽  
Shumei Mao ◽  
Yanyan Sun ◽  
Liyuan Sun ◽  
Ning Ding ◽  
...  
Keyword(s):  
Mouse Model ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Diabetic Mouse ◽  
Living Cells ◽  
Pancreatic Tissue ◽  
Diabetic Mice ◽  
Mice Model ◽  
Viscosity Changes

A mitochondria-targeted near-infrared fluorescent probe NIR-V with 700 nm emission was designed to monitor cell viscosity changes, which was applied to detect the intracellular viscosity and imagine pancreatic tissue in diabetic mouse model.

scholarly journals Flos Puerariae Extract Ameliorates Cognitive Impairment in Streptozotocin-Induced Diabetic Mice

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Zhong-he Liu ◽  
Hong-guang Chen ◽  
Pan-feng Wu ◽  
Qing Yao ◽  
Hong-ke Cheng ◽  
...  
Keyword(s):  
Body Weight ◽  
Cerebral Cortex ◽  
Cognitive Impairment ◽  
Cognitive Deficits ◽  
Ache Activity ◽  
Memory Deficits ◽  
Test Body ◽  
Morris Water Maze Test ◽  
Diabetic Mice ◽  
Mice Model

Objective. The effects of Flos Puerariae extract (FPE) on cognitive impairment associated with diabetes were assessed in C57BL/6J mice.Methods. Experimental diabetic mice model was induced by one injection of 50 mg/kg streptozotocin (STZ) for 5 days consecutively. FPE was orally administrated at the dosages of 50, 100, or 200 mg/kg/day, respectively. The learning and memory ability was assessed by Morris water maze test. Body weight, blood glucose, free fatty acid (FFA) and total cholesterol (TCH) in serum, malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and acetylcholinesterase (AChE) activities in cerebral cortex and hippocampus were also measured.Results. Oral administration of FPE significantly improved cognitive deficits in STZ-induced diabetic mice. FPE treatment also maintained body weight and ameliorated hyperglycemia and dyslipidemia in diabetic mice. Additionally, decreased MDA level, enhanced CAT, and GSH-Px activities in cerebral cortex or hippocampus, as well as alleviated AChE activity in cerebral cortex, were found in diabetic mice supplemented with FPE.Conclusion. This study suggests that FPE ameliorates memory deficits in experimental diabetic mice, at least partly through the normalization of metabolic abnormalities, ameliorated oxidative stress, and AChE activity in brain.

P0983INHIBITION OF ATF3 BY RAF INHIBITOR GW5074 ON DIABETIC NEPHROPATHY

2020 ◽  
Vol 35 (Supplement_3) ◽  
Author(s):  
Jee Young Han ◽  
Jin Joo Cha ◽  
Young Sun Kang ◽  
Jung Yeon Ghee ◽  
Ji Ae Yoo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Transcription Factor ◽  
Diabetic Nephropathy ◽  
High Glucose ◽  
Kinase Inhibitor ◽  
Protective Role ◽  
Activating Transcription Factor 3 ◽  
Diabetic Mice ◽  
Mice Model ◽  
Gene Expressions

Abstract Background and Aims Activating Transcription Factor 3 (ATF3) is a stress-adaptive transcription factor, which has been suggested to be involved in maintaining glucose homeostasis. ATF3 respond rapidly to various stimuli like high glucose, fatty acids and oxidative stress, and is observed to either protective or detrimental effects in diabetic condition. Therefore to elucidate the exact role in diabetic nephropathy of ATF3, we investigated the role of ATF3 by inhibition with Raf-inhibitor GW5047 on diabetic mice model. Method ATF3 level was examined in the mouse podocytes and NRK cells with either overexpression or downregulation with ATF3. 8 week db/m and db/db mice as the model of diabetic mice were examined for the expression of ATF3 and were treated with GW5074, a Raf1 kinase inhibitor targeting the ATF3 intraperitoneally with a dose of 0.5mg/kg for 12 weeks. Results In cultured mouse podocytes and NRK cells, high glucose and angiotensin II markedly increased ATF3 expression. Gene Expressions of NOX4, MCP-1 and NF-kB were augmented by ATF3, and were attenuated by ATF3 siRNA. In db/db mice, plasma ATF3 level was not different from control db/m, however the urinary ATF3 excretion was significantly higher. Treatment of GW5074 decreased urinary ATF3 excretion. After 12 week treatment, serum creatinine level was significantly lower in the treatment db/db group, with less systemic oxidative stress. There were no significant differences in body weight, whereas the food intake was decreased in GW5047 group. Overall lipid profile or HOMA-IR, HbA1c level was not different from each group. Serum adiponectin were otherwise increased in GW5074 group. Urinary excretion of albumin at 2 month of treatment decreased with urinary nephrin excretion. Trend of increased gene expression of JNK, p-38, smad2, ERK which was downregulated by GW5074 was noted. Conclusion These findings suggest that in diabetic condition, the activation of ATF3 is associated pathogenesis of diabetic nephropathy and targeting ATF3 may have a protective role in the disease progression.

scholarly journals Co-transplantation of Human Fetal Mesenchymal and Hematopoietic Stem Cells in Type 1 Diabetic Mice Model

2019 ◽  
Vol 10 ◽  
Author(s):  
Babak Arjmand ◽  
Parisa Goodarzi ◽  
Hamid Reza Aghayan ◽  
Moloud Payab ◽  
Fakher Rahim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Diabetic Mice ◽  
Mice Model ◽  
Hematopoietic Stem

scholarly journals ACCELERATED WOUND HEALING ABILITY OF SACRAN HYDROGEL FILM BY KERATINOCYTE GROWTH FACTOR IN ALLOXAN-INDUCED DIABETIC MICE

2018 ◽  
Vol 10 (2) ◽  
pp. 57 ◽  
Author(s):  
Nasrul Wathoni ◽  
Aliya Nur Hasanah ◽  
Ahmed Fouad Abdelwahab Mohammed ◽  
Elasari Dwi Pratiwi ◽  
Ripa’atul Mahmudah
Keyword(s):  
Wound Healing ◽  
Tensile Strength ◽  
Growth Factor ◽  
Keratinocyte Growth Factor ◽  
Research Work ◽  
Physicochemical Characterization ◽  
Swelling Ratio ◽  
Diabetic Mice ◽  
Mice Model ◽  
Hydrogel Film

Objective: The main objective of the research work was to fabricate sacran hydrogel film containing keratinocyte growth factor (Sacran/KGF-HGF), and to evaluate their wound healing ability in alloxan-induced diabetic mice model.Methods: The physicochemical characterization of Sacran/KGF-HGF were investigated by thickness, tensile strength, swelling ratio, x-ray diffractometer (XRD), scanning electron microscope (SEM), and biodegradability. The wound healing ability was investigated by creating two full-thickness excisional wounds inalloxan-induced diabetic mice.Results: The thickness, tensile strength, and swelling ratio results showed that KGF in the Sacran/KGF-HGF improved not only the thickness of sacran hydrogel film (Sacran-HGF), but also the tensile strength and swelling ability of Sacran-HGF. The XRD and SEM results confirmed that the Sacran/KGF-HGF were amorphous and similar morphology to Sacran-HGF, respectively. The biodegradability results revealed that the Sacran/KGF-HGF degraded for about 41.29% in trichloroacetic acid (TCA) and 22.92% in TrypLE™ (recombinant enzyme) solutions. In addition, KGF improved the degradability of Sacran/KGF-HGF in both solutions. Interestingly, the Sacran/KGF-HGF, which was applied on wound site, considerably improved the wound healing ability of Sacran-HGF at 6, 9 and 12 d in alloxan-induced diabetic mice model, compared to control (non-treated).Conclusion: These results suggest that KGF has the potential to promote the chronic wound healing ability of Sacran-HGF.

Allotrap prolongs survival of porcine islet xenografts in diabetic mice

1997 ◽  
Vol 29 (4) ◽  
pp. 2168-2169 ◽  
Author(s):  
E.C. Squiers ◽  
M. Hodell ◽  
R. Beulow
Keyword(s):  
Diabetic Mice ◽  
Porcine Islet

scholarly journals Antidiabetic properties of Solanum villosum and Solanum nigrum var sarrachoides in a streptozotocin-induced diabetic mice model

2019 ◽  
Vol 8 (11) ◽  
pp. 2396
Author(s):  
Samuel Nderitu Nyaga ◽  
Peter Mbaabu Mathiu ◽  
Cecilia Moraa Onyango ◽  
Gerald Otwabe Areba
Keyword(s):  
Body Weight ◽  
Solanum Nigrum ◽  
Qualitative Assessment ◽  
Diabetic Mice ◽  
Mice Model ◽  
Oral Gavage ◽  
The Family ◽  
Current Therapies ◽  
Phytochemical Constituents ◽  
Solanum Villosum

Background: Diabetes mellitus (DM) poses immense challenge to the health of people worldwide. Current therapies are limited by cost and adverse effects. Solanum nigrum, a complex of many species in the family Solanaceae has been recorded to be used by many communities in the management of DM. The aim of this study was to evaluate the phytochemical, antidiabetic efficacy and safety of two species, namely; Solanum villosum and S. nigrum var sarrachoides using streptozotocin-induced diabetic mice model.Methods: Qualitative assessment for phytochemical constituents was carried out. Acute toxicity was conducted based on ‘Organisation of Economic Cooperation and Development’ 2001 guidelines. Diabetes was induced by injection of streptozotocin at a dose of 200 mg/kg body weight intraperitoneal after the mice fasted for 8 hours. Aqueous extracts were administered orally using an oral gavage at doses of 150, and 300 mg/kg body weight for each plant daily and monitored weekly for 28 days.Results: Both plants contain vital phytochemicals. Flavonoids, alkaloids, tannins, saponins, phenols, and glycosides were present in both plants. However, phytosterols and coumarins were absent in S. villosum. Additionally, both plants did not show toxicity. Both plants showed efficacy with S. nigrum var sarrachoides being more potent at both doses.Conclusions: The study validates the use of these plants by herbalists and recommends further studies on them with the aim of elucidating the active compounds that can be used as novel therapies for diabetes. Additionally, the study recommends the evaluation of other species in this complex for antidiabetic properties.

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