scholarly journals Increased Skeletal Muscle GLUT4 Expression in Obese Mice After Voluntary Wheel Running Exercise Is Posttranscriptional

Diabetes ◽  
2016 ◽  
Vol 65 (10) ◽  
pp. 2911-2919 ◽  
Author(s):  
Jami M. Gurley ◽  
Beth A. Griesel ◽  
Ann Louise Olson
Keyword(s):  
Skeletal Muscle ◽  
Wheel Running ◽  
Obese Mice ◽  
Voluntary Wheel Running ◽  
Running Exercise ◽  
Glut4 Expression ◽  
Voluntary Wheel

scholarly journals PO-034 Effect of Voluntary Exercise on Cartilage Morphology of Knee Osteoarthritis in Obese Mice Induced by High-fat Diet

2018 ◽  
Vol 1 (3) ◽  
Author(s):  
Wenhao Zong ◽  
Yang Liu ◽  
Lulu Dai ◽  
Guijun Dong
Keyword(s):  
Articular Cartilage ◽  
Knee Osteoarthritis ◽  
High Fat Diet ◽  
Wheel Running ◽  
Cartilage Thickness ◽  
Obese Mice ◽  
High Fat ◽  
Voluntary Wheel Running ◽  
Running Exercise ◽  
Voluntary Wheel

Objective To examine the effect of voluntary wheel-running exercise on cartilage morphology of knee osteoarthritis(KOA) in obese mice induced by high-fat diet,and explore the protective role of 4 weeks voluntary wheel-running exercise on KOA,finally providing effective experimental evidence for clinical treatment of knee osteoarthritis. Methods C57BL/6J mice were randomly assigned to the C-Sed group,C-Ex group,HF-Sed group and HF-Ex group.The control groups were fed a control diet(13.5% kcal from fat),and the high-fat groups were fed a high-fat diet(60% kcal from fat).After feeding 8 weeks different diets,the exercise groups were starting running.In order to examine the effect of voluntary wheel-running exercise on cartilage morphology of KOA,the joint of knee were harvested to be fixed,decalcified and embedded in paraffin,and the four-micrometer-thick sections were stained with both HE and toluidine blue . Results After feeding twelve weeks different diets,the body mass of the high-fat diet group mice has a significant increase,which demonstrates that high-fat diet could successfully induce the mice obese.From the results of HE and toluidine blue,in comparison to the C-Sed group,the surface of the knee articular cartilage in the HF-Sed group was not intact and smooth,and the thickness of articular cartilage has a significant decrease（p<0.001）;contrary to the HF-Sed group,the surface of the knee articular cartilage in HF-Ex group was slightly smooth,and there was significant increase in cartilage thickness. Conclusions Four weeks voluntary wheel-running exercise can increase cartilage thickness ,decrease the Mankin’s score and delay the degeneration of knee cartilage in obese mice.To conclude,the short-term wheel-running exercise protects against obesity-induced KOA.

scholarly journals Metabolic Adaptations of Skeletal Muscle to Voluntary Wheel Running Exercise in Hypertensive Heart Failure Rats

2013 ◽  
pp. 361-369 ◽  
Author(s):  
R. L. SCHULTZ ◽  
E. L. KULLMAN ◽  
R. P. WATERS ◽  
H. HUANG ◽  
J. P. KIRWAN ◽  
...  
Keyword(s):  
Heart Failure ◽  
Skeletal Muscle ◽  
Citrate Synthase ◽  
Wheel Running ◽  
Heart Function ◽  
Oxidative Capacity ◽  
Voluntary Wheel Running ◽  
Spontaneously Hypertensive ◽  
Running Exercise ◽  
Voluntary Wheel

The Spontaneously Hypertensive Heart Failure (SHHF) rat mimics the human progression of hypertension from hypertrophy to heart failure. However, it is unknown whether SHHF animals can exercise at sufficient levels to observe beneficial biochemical adaptations in skeletal muscle. Thirty-seven female SHHF and Wistar-Furth (WF) rats were randomized to sedentary (SHHFsed and WFsed) and exercise groups (SHHFex and WFex). The exercise groups had access to running wheels from 6-22 months of age. Hindlimb muscles were obtained for metabolic measures that included mitochondrial enzyme function and expression, and glycogen utilization. The SHHFex rats ran a greater distance and duration as compared to the WFex rats (P<0.05), but the WFex rats ran at a faster speed (P<0.05). Skeletal muscle citrate synthase and β-hydroxyacyl-CoA dehydrogenase enzyme activity was not altered in the SHHFex group, but was increased (P<0.05) in the WFex animals. Citrate synthase protein and gene expression were unchanged in SHHFex animals, but were increased in WFex rats (P<0.05). In the WFex animals muscle glycogen was significantly depleted after exercise (P<0.05), but not in the SHHFex group. We conclude that despite robust amounts of aerobic activity, voluntary wheel running exercise was not sufficiently intense to improve the oxidative capacity of skeletal muscle in adult SHHF animals, indicating an inability to compensate for declining heart function by improving peripheral oxidative adaptations in the skeletal muscle.

scholarly journals Voluntary Wheel Running Selectively Augments Insulin-Stimulated Vasodilation in Arterioles from White Skeletal Muscle of Insulin-Resistant Rats

2012 ◽  
Vol 19 (8) ◽  
pp. 729-738 ◽  
Author(s):  
Catherine R. Mikus ◽  
Bruno T. Roseguini ◽  
Grace M. Uptergrove ◽  
E. Matthew Morris ◽  
Randy Scott Rector ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Wheel Running ◽  
Voluntary Wheel Running ◽  
Insulin Resistant ◽  
Voluntary Wheel

scholarly journals Five months of voluntary wheel running downregulates skeletal muscle LINE-1 gene expression in rats

2019 ◽  
Vol 317 (6) ◽  
pp. C1313-C1323 ◽  
Author(s):  
Matthew A. Romero ◽  
Petey W. Mumford ◽  
Paul A. Roberson ◽  
Shelby C. Osburn ◽  
Hailey A. Parry ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Dna Methylation ◽  
Exercise Training ◽  
Mrna Expression ◽  
Wheel Running ◽  
Mobile Dna ◽  
Voluntary Wheel Running ◽  
L6 Myotubes ◽  
Voluntary Wheel

Transposable elements (TEs) are mobile DNA and constitute approximately half of the human genome. LINE-1 (L1) is the only active autonomous TE in the mammalian genome and has been implicated in a number of diseases as well as aging. We have previously reported that skeletal muscle L1 expression is lower following acute and chronic exercise training in humans. Herein, we used a rodent model of voluntary wheel running to determine whether long-term exercise training affects markers of skeletal muscle L1 regulation. Selectively bred high-running female Wistar rats ( n = 11 per group) were either given access to a running wheel (EX) or not (SED) at 5 wk of age, and these conditions were maintained until 27 wk of age. Thereafter, mixed gastrocnemius tissue was harvested and analyzed for L1 mRNA expression and DNA content along with other L1 regulation markers. We observed significantly ( P < 0.05) lower L1 mRNA expression, higher L1 DNA methylation, and less L1 DNA in accessible chromatin regions in EX versus SED rats. We followed these experiments with 3-h in vitro drug treatments in L6 myotubes to mimic transient exercise-specific signaling events. The AMP-activated protein kinase (AMPK) agonist 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR; 4 mM) significantly decreased L1 mRNA expression in L6 myotubes. However, this effect was not facilitated through increased L1 DNA methylation. Collectively, these data suggest that long-term voluntary wheel running downregulates skeletal muscle L1 mRNA, and this may occur through chromatin modifications. Enhanced AMPK signaling with repetitive exercise bouts may also decrease L1 mRNA expression, although the mechanism of action remains unknown.

Effects of voluntary wheel running and amino acid supplementation on skeletal muscle of mice

2004 ◽  
Vol 93 (5-6) ◽  
pp. 655-664 ◽  
Author(s):  
Maria Antonietta Pellegrino ◽  
Lorenza Brocca ◽  
Francesco Saverio Dioguardi ◽  
Roberto Bottinelli ◽  
Giuseppe D’Antona
Keyword(s):  
Skeletal Muscle ◽  
Amino Acid ◽  
Wheel Running ◽  
Amino Acid Supplementation ◽  
Voluntary Wheel Running ◽  
Acid Supplementation ◽  
Voluntary Wheel
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