scholarly journals Stromal Cell Derived Factor-1 (SDF-1)/CXCL12 Attenuates Diabetes in Mice and Promotes Pancreatic  -Cell Survival by Activation of the Prosurvival Kinase Akt

Diabetes ◽  
2007 ◽  
Vol 56 (12) ◽  
pp. 2946-2957 ◽  
Author(s):  
T. Yano ◽  
Z. Liu ◽  
J. Donovan ◽  
M. K. Thomas ◽  
J. F. Habener
Keyword(s):  
Cell Survival ◽  
Stromal Cell ◽  
Pancreatic Cell ◽  
Diabetes In Mice ◽  
Stromal Cell Derived Factor ◽  
Prosurvival Kinase

scholarly journals A Naturally Occurring Splice Variant of CXCL12/Stromal Cell-Derived Factor 1 Is a Potent Human Immunodeficiency Virus Type 1 Inhibitor with Weak Chemotaxis and Cell Survival Activities

2007 ◽  
Vol 81 (15) ◽  
pp. 8140-8148 ◽  
Author(s):  
Jeffrey D. Altenburg ◽  
Hal E. Broxmeyer ◽  
Qingwen Jin ◽  
Scott Cooper ◽  
Sunanda Basu ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Cell Survival ◽  
Stromal Cell ◽  
Splice Variants ◽  
Biological Properties ◽  
Affinity Column ◽  
Immunodeficiency Virus ◽  
Antiviral Activities ◽  
Stromal Cell Derived Factor

ABSTRACT CXCL12/stromal cell-derived factor 1 is a member of the CXC family of chemokines that plays an important role in hematopoiesis and signals through CXCR4 and CXCR7. Two splice variants of human CXCL12 (CXCL12α and CXCL12β) induce chemotaxis of CXCR4+ cells and inhibit X4 infection. Recent studies described four other novel splice variants of human CXCL12; however, their antiviral activities were not investigated. We constructed and expressed all of the CXCL12 splice variants in Escherichia coli. Recombinant proteins were purified through a His affinity column, and their biological properties were analyzed. All six CXCL12 variants induced chemotaxis of CXCR4+ and CXCR7+ cell lines. Enhancement of survival and replating capacity of human hematopoietic progenitor cells were observed with CXCL12α, CXCL12β, and CXCL12ε but not with the other variants. CXCL12γ showed the greatest antiviral activity in X4 inhibition assays and the weakest chemotaxis activity through CXCR4. The order of potency in X4 inhibition assays was as follows: CXCL12γ > CXCL12β > CXCL12α > CXCL12θ > CXCL12ε > CXCL12δ. The order of anti-human immunodeficiency virus (HIV) activity was associated with the number of BBXB motifs present in each variant; the most potent inhibitor was CXCL12γ, with five BBXB domains. The results suggest that the different C termini of CXCL12 variants may contain important molecular determinants for the observed differences in antiviral effects and other biological functions. These studies implicate CXCL12γ as a potent HIV-1 entry inhibitor with significantly reduced chemotaxis activity and small or absent effects on progenitor cell survival or replating capacity, providing important insight into the structure-function relationships of CXCL12.

scholarly journals Stromal cell-derived factor-1 (SDF-1) signalling regulates human placental trophoblast cell survival

2004 ◽  
Vol 10 (12) ◽  
pp. 901-909 ◽  
Author(s):  
Mambarath A. Jaleel ◽  
Amy C. Tsai ◽  
Sumita Sarkar ◽  
Paula V. Freedman ◽  
Lewis P. Rubin
Keyword(s):  
Cell Survival ◽  
Stromal Cell ◽  
Trophoblast Cell ◽  
Stromal Cell Derived Factor

scholarly journals Stromal Cell-Derived Factor-1β Mediates Cell Survival through Enhancing Autophagy in Bone Marrow-Derived Mesenchymal Stem Cells

PLoS ONE ◽  
2013 ◽  
Vol 8 (3) ◽  
pp. e58207 ◽  
Author(s):  
Samuel Herberg ◽  
Xingming Shi ◽  
Maribeth H. Johnson ◽  
Mark W. Hamrick ◽  
Carlos M. Isales ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cell Survival ◽  
Stromal Cell ◽  
Stromal Cell Derived Factor
Sign in / Sign up

Export Citation Format

Share Document