scholarly journals Flow Cytometry for DNA Contents of Somatic Cells and Spermatozoa in the Progeny of Natural Tetraploid Loach

1996 ◽  
Vol 62 (6) ◽  
pp. 870-877 ◽  
Author(s):  
Quanqi Zhang ◽  
Katsutoshi Arai
Keyword(s):  
Flow Cytometry ◽  
Somatic Cells ◽  
Dna Contents

scholarly journals Chromosomal Composition of Aneuploid Clones with Different DNA Contents in Head and Neck Squamous Cell Carcinomas as Determined by Combined Flow Cytometry and FluorescenceIn SituHybridization

2000 ◽  
Vol 20 (4) ◽  
pp. 197-203 ◽  
Author(s):  
Joerg Hemmer ◽  
Carmen Hauser
Keyword(s):  
Flow Cytometry ◽  
Head And Neck ◽  
Squamous Cell ◽  
Squamous Cell Carcinomas ◽  
Dna Probes ◽  
Chromosome 17 ◽  
Cell Populations ◽  
Dna Flow Cytometry ◽  
Wide Range ◽  
Dna Contents

Studies with DNA flow cytometry (FCM) have shown that DNA contents of aneuploid tumour clones vary in a wide range. The aim of this study was to analyse whether homologous chromosomal changes exist despite the individual differences that may be of general relevance for the development of gross aneuploidy in squamous cell carcinomas of the head and neck. Fluorescencein situhybridization (FISH) with 13 centromere‐specific DNA probes was applied to 3 diploid and 11 aneuploid tumours with DNA indices ranging between 0.8 and 2.2. Disomic and monosomic cell populations were prevalent findings in DNA‐diploid tumours. Polysomies were common in aneuploid tumours. Different degrees of aneusomy for identical chromosomes were recurrent features in aneuploid tumours. FISH signal heterogeneity was identified for all chromosomes. The mean number of aneusomic cell populations identified for DNA‐aneuploid tumours ranged between 1.6 for chromosome 17 and 3.1 for chromosome 3. Inconsistencies between FISH and FCM data may indicate that centromere‐specific DNA probes identify gains and losses of marker DNA due to complex karyotypic rearrangements rather than absolute changes in chromosome numbers. Overall, there was no evidence of the critical involvement of particular chromosomes in the development of different DNA contents.

scholarly journals Genome Sizes in Hepatica Mill: (Ranunculaceae) Show a Loss of DNA, Not a Gain, in Polyploids

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
B. J. M. Zonneveld
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Dna Content ◽  
Close Relationships ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Dna Contents ◽  
Asiatic Species ◽  
Nuclear Dna Contents ◽  
Allotetraploid Species

Genome size (C-value) was applied anew to investigate the relationships within the genus Hepatica (Ranunculaceae). More than 50 samples representing all species (except H. falconeri), from wild and cultivated material, were investigated. Species of Hepatica turn out to be diploid (), tetraploid ( ), and a possible pentaploid. The somatic nuclear DNA contents (2C-value), as measured by flow cytometry with propidium iodide, were shown to range from 33 to 80 pg. The Asiatic and American species, often considered subspecies of H. nobilis, could be clearly distinguished from European H. nobilis. DNA content confirmed the close relationships in the Asiatic species, and these are here considered as subspecies of H. asiatica. Parents for the allotetraploid species could be suggested based on their nuclear DNA content. Contrary to the increase in genome size suggested earlier for Hepatica, a significant (6%–14%) loss of nuclear DNA in the natural allopolyploids was found.

scholarly journals Flow cytometry measurement of the DNA contents of G0/G1 diploid cells from three different teleost fish species

Cytometry ◽  
2002 ◽  
Vol 48 (1) ◽  
pp. 20-25 ◽  
Author(s):  
Juana Ciudad ◽  
Elena Cid ◽  
Almudena Velasco ◽  
Juan M. Lara ◽  
José Aijón ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Fish Species ◽  
Teleost Fish ◽  
Dna Contents ◽  
Diploid Cells

scholarly journals Flow Cytometry and Flow Sorting of Metaphase Chromosomes from the Dasyurid Marsupial Dasyurus viverrinus

1985 ◽  
Vol 38 (4) ◽  
pp. 377 ◽  
Author(s):  
Brandon Wainwright ◽  
Rory Hope
Keyword(s):  
Flow Cytometry ◽  
Chromosomal Dna ◽  
Metaphase Chromosomes ◽  
Homogeneous Groups ◽  
Flow Sorting ◽  
Dna Contents ◽  
Conventional Methods

Metaphase chromosomes (2n = 14) from D. viverrinus were analysed by flow cytometry and flow sorted into six homogeneous groups. Relative chromosomal DNA contents and distribution frequencies of the groups corresponded closely with values for the karyotype obtained by conventional methods.

COMPARISON OF PLANT DNA CONTENTS DETERMINED BY FEULGEN MICROSPECTROPHOTOMETRY AND LASER FLOW CYTOMETRY

1991 ◽  
Vol 78 (2) ◽  
pp. 183-188 ◽  
Author(s):  
Martin J. Michaelson ◽  
H. James Price ◽  
John R. Ellison ◽  
J. Spencer Johnston
Keyword(s):  
Flow Cytometry ◽  
Plant Dna ◽  
Dna Contents

scholarly journals An approach on the in vitro maintenance of sugarcane with views for conservation and monitoring of plant nuclear DNA contents via flow cytometry

2014 ◽  
Vol 51 (2) ◽  
pp. 220-230 ◽  
Author(s):  
Gabriela Ferreira Nogueira ◽  
Leila Aparecida Salles Pio ◽  
Moacir Pasqual ◽  
Adriane Amaral ◽  
Jonny Everson Scherwinski-Pereira
Keyword(s):  
Flow Cytometry ◽  
Nuclear Dna ◽  
Dna Contents ◽  
Nuclear Dna Contents ◽  
In Vitro Maintenance
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