scholarly journals Chromosomal Composition of Aneuploid Clones with Different DNA Contents in Head and Neck Squamous Cell Carcinomas as Determined by Combined Flow Cytometry and FluorescenceIn SituHybridization

2000 ◽  
Vol 20 (4) ◽  
pp. 197-203 ◽  
Author(s):  
Joerg Hemmer ◽  
Carmen Hauser
Keyword(s):  
Flow Cytometry ◽  
Head And Neck ◽  
Squamous Cell ◽  
Squamous Cell Carcinomas ◽  
Dna Probes ◽  
Chromosome 17 ◽  
Cell Populations ◽  
Dna Flow Cytometry ◽  
Wide Range ◽  
Dna Contents

Studies with DNA flow cytometry (FCM) have shown that DNA contents of aneuploid tumour clones vary in a wide range. The aim of this study was to analyse whether homologous chromosomal changes exist despite the individual differences that may be of general relevance for the development of gross aneuploidy in squamous cell carcinomas of the head and neck. Fluorescencein situhybridization (FISH) with 13 centromere‐specific DNA probes was applied to 3 diploid and 11 aneuploid tumours with DNA indices ranging between 0.8 and 2.2. Disomic and monosomic cell populations were prevalent findings in DNA‐diploid tumours. Polysomies were common in aneuploid tumours. Different degrees of aneusomy for identical chromosomes were recurrent features in aneuploid tumours. FISH signal heterogeneity was identified for all chromosomes. The mean number of aneusomic cell populations identified for DNA‐aneuploid tumours ranged between 1.6 for chromosome 17 and 3.1 for chromosome 3. Inconsistencies between FISH and FCM data may indicate that centromere‐specific DNA probes identify gains and losses of marker DNA due to complex karyotypic rearrangements rather than absolute changes in chromosome numbers. Overall, there was no evidence of the critical involvement of particular chromosomes in the development of different DNA contents.

Treatment of primary mucosal head and neck squamous cell carcinoma using photodynamic therapy: results after 25 treated cases

2003 ◽  
Vol 117 (9) ◽  
pp. 713-717 ◽  
Author(s):  
M. G. Dilkes ◽  
E. Benjamin ◽  
S. Ovaisi ◽  
A. S. Banerjee
Keyword(s):  
Squamous Cell Carcinoma ◽  
Photodynamic Therapy ◽  
Head And Neck ◽  
Squamous Cell ◽  
Complete Response ◽  
Primary Treatment ◽  
Squamous Cell Carcinomas ◽  
Radical Radiotherapy ◽  
Wide Range

The use of photodynamic therapy for the treatment of malignant and non-malignant conditions is increasing. This paper demonstrates the efficacy of a second-generation photosensitizer, Foscan®, in the primary treatment of a wide range of mucosal head and neck squamous cell carcinomas. Tumours ranged in stage from T1 to T3. A complete response to primary treatment was seen in 19/21 patients (90 per cent). In laryngeal cancer recurrent after radical radiotherapy, one out of four patients treated obtained a complete response (25 per cent). Six patients (24 per cent) required surgery after photodynamic therapy, for local recurrence or dysplasia. Mean follow up was for 27.3 months (standard deviation 20.6 months).

Part I. DNA Flow Cytometry as a Prognostic Indicator in Head and Neck Cancer

1987 ◽  
Vol 96 (4) ◽  
pp. 307-318 ◽  
Author(s):  
Manning M. Goldsmith ◽  
David H. Cresson ◽  
Larry A. Arnold ◽  
Duncan S. Postma ◽  
Frederic B. Askin ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Head And Neck Cancer ◽  
Head And Neck ◽  
Prognostic Indicator ◽  
Squamous Cell Carcinomas ◽  
Dna Flow Cytometry ◽  
Dna Amount ◽  
Favorable Prognosis ◽  
Dna Amounts

The prognostic significance of deoxyribonucleic acid (DNA) flow cytometry has been investigated for many solid tumors, but few data have been accumulated for squamous cell carcinomas of the head and neck. To our knowledge, we report the largest number of patients (69) with head and neck primary carcinomas to be studied by DNA flow cytometry. In the first part of this study, we reviewed 109 consecutive patients with laryngeal or hypopharyngeal primary carcinomas which were treated at North Carolina Memorial Hospital during the period of 1981 to 1984. The final analysis comprised 139 DNA histograms (mean coefficient of variation: 8.02) on paraffin-embedded specimens from 48 patients. Of the 48 patients with primary carcinomas, 24 had glottic, 18 had supraglottic, and 6 had carcinomas from the piriform sinus. Patients had follow-up for a minimum of 12 months, with a mean follow-up period of 23 months. Twenty-three of the 48 primary carcinomas (48%) were clearly aneuploid, and the remaining 52% were tetraploid (22%) or diploid (30%). We have concluded that patients with clearly aneuploid primary carcinomas had significantly better prognoses than those with diploid tumors ( p = 0.008). High DNA amounts (greater than 40% of cells beyond the diploid peak, DNA G1GO) also correlated with a favorable prognosis when compared with low DNA amounts ( p <0.01), and this remained significant when the clinical outcome was adjusted for staging of the primary site (T), nodal status, and stage of disease. Ploidy was the most significant prognostic variable for the laryngeal group of patients. In the second part of the study, twenty-one patients with oral cavity squamous cell carcinomas were studied in a similar fashion as the group with laryngeal carcinomas. In this group, a low DNA amount, with 40% as the cutoff point, was associated with a favorable prognosis ( p = 0.024), and this remained significant while controlling for I, nodal status, and stage of disease. Numbers were too small to permit evaluation of the impact of ploidy in this group, but there was a slight trend toward aneuploidy and tetraploidy, correlating with a poor treatment outcome ( p = 0.228). DNA amount was the most significant prognostic variable for the group of patients with oral cavity carcinomas. We conclude that DNA flow cytometry may be a powerful prognostic indicator in malignant conditions of the head and neck. The implications of these data for the management of head and neck cancer are discussed.

Nuclear DNA content using computerized image cytometry of squamous cell carcinomas of the head and neck

1997 ◽  
Vol 111 (1) ◽  
pp. 43-47 ◽  
Author(s):  
S. K. Sarker ◽  
K. Ghufoor ◽  
K. S. Patel ◽  
N. S. Tolley ◽  
D. V. Coleman
Keyword(s):  
Flow Cytometry ◽  
Head And Neck ◽  
Squamous Cell ◽  
Nuclear Dna ◽  
Clinical Stage ◽  
Nuclear Dna Content ◽  
Image Cytometry ◽  
Squamous Cell Carcinomas ◽  
Histological Differentiation ◽  
Ploidy Status

AbstractPloidy status using flow cytometry of head and neck cancers may be of prognostic value. We describe the use of image cytometry in ploidy measurement of squamous cell carcinomas of the head and neck (SCCHN). This technique allows only tumour cells to be measured, thereby rejecting debris, artefact and benign cells. Tissue sections were cut from tumours and then Feulgen stained. A total of 60 patients were included in this study, 23 females and 37 males. The data reveals a relationship between ploidy status and the histological differentiation. However, the ploidy status and histological differentiation do not appear to correlate to the clinical stage of the disease. This method of measuring ploidy may be more accurate than flow cytometry and may have a prognostic role in head and neck cancer patients. A study comparing both methods may demonstrate this and we aim to evaluate this in the future.

In vitro proliferation of primary human head and neck squamous cell carcinomas evaluated by flow cytometry

1987 ◽  
Vol 36 (1) ◽  
pp. 71-81 ◽  
Author(s):  
J.Th. Bijman ◽  
D.J.Th. Wagener ◽  
J.M.C. Wessels ◽  
D. Elprana ◽  
P. van den Broek
Keyword(s):  
Flow Cytometry ◽  
Head And Neck ◽  
Squamous Cell ◽  
Human Head ◽  
Squamous Cell Carcinomas ◽  
In Vitro Proliferation

scholarly journals Distinctive chromosomal instability patterns in oral verrucous and squamous cell carcinomas detected by high-resolution DNA flow cytometry

Cancer ◽  
2011 ◽  
Vol 117 (22) ◽  
pp. 5052-5057 ◽  
Author(s):  
Monica Pentenero ◽  
Alessandra Donadini ◽  
Emanuela Di Nallo ◽  
Massimo Maffei ◽  
Roberto Marino ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
High Resolution ◽  
Squamous Cell ◽  
Chromosomal Instability ◽  
Squamous Cell Carcinomas ◽  
Dna Flow Cytometry

Relationship between histologic features, DNA flow cytometry, and clinical behavior of squamous cell carcinomas of the larynx

Cancer ◽  
1991 ◽  
Vol 67 (1) ◽  
pp. 141-149 ◽  
Author(s):  
Savino Ruá ◽  
Alberto Comino ◽  
Adriana Fruttero ◽  
Giovanni Cera ◽  
Carlo Semeria ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Squamous Cell ◽  
Squamous Cell Carcinomas ◽  
Dna Flow Cytometry ◽  
Clinical Behavior

Smoking correlates with the presence of p53 mutations in squamous cell carcinomas of the head and neck (SCCHN)

1998 ◽  
Vol 23 (3) ◽  
pp. 268-269
Author(s):  
Liloglou ◽  
Scholes ◽  
Spandidos ◽  
Jones ◽  
Vaughan ◽  
...  
Keyword(s):  
Head And Neck ◽  
Squamous Cell ◽  
Squamous Cell Carcinomas ◽  
P53 Mutations

Identification of the Genomic Structure of Tumor Suppressor ING1 and Mutation Analysis in Head and Neck Squamous Cell Carcinomas

2005 ◽  
Vol 14 (2) ◽  
pp. 320-321
Author(s):  
Mehmet Gunduz ◽  
Esra Gunduz ◽  
Byung-Moo Min ◽  
Gene Lee ◽  
Ji-Jun Lim ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Head And Neck ◽  
Mutation Analysis ◽  
Squamous Cell ◽  
Genomic Structure ◽  
Squamous Cell Carcinomas
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