Histopathological study on the healing of experimentally produced bone defects following decalcified allogeneic bone matrix powder grafts in dogs.

1987 ◽  
Vol 29 (3) ◽  
pp. 749-787
Author(s):  
Sakae SEKIYA
Keyword(s):  
Bone Matrix ◽  
Bone Defects ◽  
Histopathological Study ◽  
Allogeneic Bone ◽  
Matrix Powder

scholarly journals Evaluation of in vitro biocompatibility of scaffolds for the repair of bone defects

2021 ◽  
Vol 9 (2) ◽  
Author(s):  
N. Bezdieniezhnykh ◽  
◽  
Ye. Holiuk ◽  
S. Gerasymenko ◽  
K. Saulenko ◽  
...  
Keyword(s):  
Proliferative Activity ◽  
Platelet Rich Plasma ◽  
Human Fibroblasts ◽  
Bone Matrix ◽  
Bone Defects ◽  
Allogeneic Bone ◽  
Local Bone ◽  
Heat Treated ◽  
Bank Technology

The use of bone scaffolds in traumatology and orthopedics is an extremely important issue. The growing number of cases of significant bone defects, in particular after revision arthroplasty, combat trauma and due to the introduction of new methods of reconstructive surgery of bones and joints, requires more detailed studies of the using different osteoplastic materials. Materials and methods. As scaffolds used 4 types of materials that are most often used in the clinic for the correction of bone defects - ceramic hydroxylapatite, beta-tricalcium phosphate, allogeneic bone matrix treated with gamma irradiation, allogeneic bone matrix scaffold. The effect of matrices on the viability of normal human fibroblasts (M19 cell line) in cell culture in vitro was studied. The viability of cells after their co-cultivation with scaffolds was determined by colorimetric method by staining with crystal violet. To obtain an osteoinductive effect used platelet-rich plasma (PRP), standardized by the method of Araki with some modifications. The proliferative activity of fibroblasts was assessed by the level of expression of the proliferation marker Ki-67 by immunocytochemical analysis. Results. It was found that the least pronounced antiproliferative effect is shown by allogeneic bone matrix treated with gamma irradiation. Data on the complex effect of co-cultivation of fibroblasts with scaffolds in the presence of PRP on cell viability and proliferative activity were obtained. It was found that PRP improves the survival of fibroblasts by 15-30 % and increases their proliferative activity by 35-75 %. Delipidization of scaffold from allogeneic bone matrix, heat-treated by local bone bank technology, increased its biocompatibility with human fibroblasts. Conclusions. According to the results of a comparative analysis of the impact of different scaffolds on the viability of normal human fibroblasts, it was found that scaffolds from allogeneic bone matrix have the least pronounced antiproliferative effect. Platelet-rich plasma has been shown to improve fibroblast survival and increase their proliferative activity. Treatment with 70 % ethyl alcohol scaffold from allogeneic bone matrix, heat-treated by local bone bank technology, increased its biocompatibility with human fibroblasts.

ALLOGRAFT BONE APPLICATION IN THE RAT CALVARIAL BONE DEFECT MODEL: A HISTOPATHOLOGICAL STUDY

2021 ◽  
pp. 22-24
Author(s):  
Büşra Deveci ◽  
Ahmet Dağ ◽  
Firat Asir ◽  
Ebru Gökalp Özkorkmaz ◽  
Engin Deveci
Keyword(s):  
Bone Defect ◽  
Bone Matrix ◽  
Scar Tissue ◽  
Bone Defects ◽  
Defect Group ◽  
Male Rats ◽  
Histopathological Study ◽  
Defect Model ◽  
Calvarial Bone ◽  
Calvarial Bone Defect

Trauma, neoplasms, infections, and congenital anomalies may be the reason for the calvarial bone defects. For eliminating bone defects in the cranial region to stimulate bone regeneration different graft types have been tried. In our study, we aimed to investigate the effects of allograft application in the rat calvarial bone defect model. For this purpose, 14 Wistar male rats were determined; defect (n=7) and defect + graft (n=7) groups. . The frontal bone was opened and a circular full thickness bone defect (5 mm) was created in the midline. Allograft material was placed in the defect area. All animals were sacriced after 28 days and the calvarial bones were followed up for routine histologic preparations. Sections were stained with H-E and scoring for histopathological parameters (inammation, brosis, osteoclast number, osteoblast number, osteocyte number, matrix formation, new bone trabecular diameter). In our study, inammation, brosis and osteoclast numbers decreased in the defect + graft group compared to the defect group, and osteocyte, osteoblast, matrix formation and bone trabecular diameter has increased signicantly. Histopathological evaluation revealed scar tissue, increased mononuclear cell inltration, and necrosis in the defect group. In the defect + graft group, an increase in collagen ber, a decrease in inammatory cells, an increase in osteoblast cells and bone matrix were observed. As a result, allograft application has been found to support new bone formation in the calvarial defect model by creating an osteoinductive and osteoconductive effect.

Effect of mesoporous silica and its combination with hydroxyapatite on the regeneration of rabbit’s bone defects: A pilot study

2021 ◽  
pp. 1-14
Author(s):  
Mario Pérez Sayans ◽  
Berta Rivas Mundiña ◽  
Cintia M. Chamorro Petronacci ◽  
Abel García García ◽  
Francisco José Gómez García ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Surgical Techniques ◽  
Biological Response ◽  
Bone Defects ◽  
Powdered Material ◽  
Histopathological Study ◽  
Radiographic Images ◽  
Osteogenic Response ◽  
Rabbit Tibia ◽  
Hybrid Scaffolds

BACKGROUND: Bone volume augmentation is a routine technique used in oral implantology and periodontology. Advances in the surgical techniques and the biomaterials field have allowed a greater accessibility to these treatments. Nevertheless, dehiscence and fenestrations incidence during dental implant procedures are still common in patients with bone loss. OBJECTIVE: The main objective is to evaluate in a pilot experimental study the biological response to mesoporous silica (MS) hybrid scaffolds and its regenerative capacity in different formulations. METHODS: Two defects per rabbit tibia were performed (one for control and other for test) and the biomaterials tested in this study have been used to fill the bone defects, prepared in two different formulations (3D hybrid scaffolds or powdered material, in 100% pure MS form, or 50% MS with 50% hydroxyapatite (HA). Euthanasia was performed 4 months after surgery for bone histopathological study and radiographic images were acquired by computerized microtomography. RESULTS: Results showed that radiographically and histopathologically pure MS formulations lead to a lower biological response, e.g when formulated with HA, the osteogenic response in terms of osteoconduction was greater. CONCLUSIONS: We observed tolerance and lack of toxicity of the MS and HA, without registering any type of local or systemic allergic reaction.

Accelerated repair of cortical bone defects using a synthetic extracellular matrix to deliver human demineralized bone matrix

2006 ◽  
Vol 24 (7) ◽  
pp. 1454-1462 ◽  
Author(s):  
Yanchun Liu ◽  
Shama Ahmad ◽  
Xiao Zheng Shu ◽  
R. Kent Sanders ◽  
Sally Anne Kopesec ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cortical Bone ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Bone Defects ◽  
Demineralized Bone

Enhancement of Bone Healing by Local Administration of Carbon Nanotubes Functionalized with Sodium Hyaluronate in Rat Tibiae

2017 ◽  
Vol 204 (3-4) ◽  
pp. 137-149 ◽  
Author(s):  
Vanessa B. Andrade ◽  
Marcos A. Sá ◽  
Renato M. Mendes ◽  
Paulo A. Martins-Júnior ◽  
Gerluza A.B. Silva ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Bone Repair ◽  
Bone Matrix ◽  
Sodium Hyaluronate ◽  
Repair Process ◽  
Bone Defects ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein ◽  
Bone Trabeculae ◽  
Endothelial Growth Factor

It has been reported that carbon nanotubes (CNTs) serve as nucleation sites for the deposition of bone matrix and cell proliferation. Here, we evaluated the effects of multi-walled CNTs (MWCNTs) on bone repair of rat tibiae. Furthermore, because sodium hyaluronate (HY) accelerates bone restoration, we associated CNTs with HY (HY-MWCNTs) in an attempt to boost bone repair. The bone defect was created by a 1.6-mm-diameter drill. After 7 and 14 days, tibiae were processed for histological and morphometric analyses. Immunohistochemistry was used to evaluate the expression of vascular endothelial growth factor (VEGF) in bone defects. Expression of osteocalcin (OCN), bone morphogenetic protein-2 (BMP-2), and collagen I (Col I) was assessed by real-time PCR. Histomorphometric analysis showed a similar increase in the percentage of bone trabeculae in tibia bone defects treated with HY and HY-MWCNTs, and both groups presented more organized and thicker bone trabeculae than nontreated defects. Tibiae treated with MWCNTs or HY- MWCNTs showed a higher expression of VEGF. Treatment with MWCNTs or HY-MWCNTs increased the expression of molecules involved in the bone repair process, such as OCN and BMP-2. Also, HY- and MWCNT-treated tibiae had an increased expression of Col I. Thus, it is tempting to conclude that CNTs associated or not with other materials such as HY emerged as a promising biomaterial for bone tissue engineering.

Autolysed antigen-extracted allogeneic bone for repair of diaphyseal bone defects in rabbits

1990 ◽  
Vol 31 (3) ◽  
pp. 251 ◽  
Author(s):  
Chang Dong Han ◽  
Ho Jung Kang ◽  
Eung Shick Kang ◽  
Kyoo Ho Shin ◽  
Nam Hyun Kim
Keyword(s):  
Bone Defects ◽  
Allogeneic Bone ◽  
Diaphyseal Bone
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