scholarly journals A Quantitative Study on Free Cholesterol, Cholesterol Ester and Total Cholesterol Content of Human Gingiva in Different Degrees of Chronic Marginal Periodontitis

1976 ◽  
Vol 18 (2) ◽  
pp. 165-171
Author(s):  
Koichi ITO
Keyword(s):  
Total Cholesterol ◽  
Quantitative Study ◽  
Cholesterol Ester ◽  
Free Cholesterol ◽  
Cholesterol Content ◽  
Human Gingiva ◽  
Marginal Periodontitis

Effect of Pluronic L81, a hydrophobic surfactant, on intestinal mucosal cholesterol homeostasis

1991 ◽  
Vol 261 (2) ◽  
pp. G256-G262 ◽  
Author(s):  
C. Pool ◽  
D. F. Nutting ◽  
W. J. Simmonds ◽  
P. Tso
Keyword(s):  
Total Cholesterol ◽  
Intestinal Mucosa ◽  
Free Cholesterol ◽  
Sodium Taurocholate ◽  
Cholesterol Content ◽  
Cholesterol Homeostasis ◽  
Triglyceride Rich Lipoprotein ◽  
Specific Activities ◽  
Chylomicron Formation

Addition of triglyceride and phospholipid to sodium taurocholate when chylomicron output was blocked by L81 did not increase lymphatic total cholesterol output or mucosal unesterified (UC) content more than with sodium taurocholate alone, but mucosal esterified cholesterol (CE) was increased slightly. In these animals with defective chylomicron formation, excess cholesterol accumulated in the intestinal mucosa mainly as CE. The mucosal cholesterol content of animals with normal chylomicron transport expanded during cholesterol and triglyceride absorption, and the expansion led to increased lymphatic secretion of CE. These animals accumulated significantly less CE in their mucosa than did rats treated with L81, but had about the same amount of mucosal UC. However, the overall uptake of cholesterol from the lumen, as determined by either radioactivity or mass of cholesterol in mucosa and lymph, was significantly less in the L81 rats. Also, more radioactive cholesterol remained in the lumen of the L81-treated rats. Finally, the data on specific activities of free and esterified cholesterol showed that the mucosal cholesterol derived from the lumen does not mix evenly with the free cholesterol pool in the enterocytes and is preferentially esterified for export in lymph as triglyceride-rich lipoprotein.

Lipid composition of the subcellular elements, and mitochondrial respiration of the heart and liver in hypercholesterolemic rabbits

1968 ◽  
Vol 46 (6) ◽  
pp. 823-828 ◽  
Author(s):  
C. C. Huang ◽  
K. Kako
Keyword(s):  
Total Cholesterol ◽  
Lipid Composition ◽  
Cholesterol Ester ◽  
Respiratory Control ◽  
Cholesterol Content ◽  
Liver Mitochondria ◽  
High Cholesterol Diet ◽  
Normal Ranges ◽  
Cell Fractions ◽  
Altered Lipid

Rabbits were fed a diet containing 3% cholesterol for 2–6 months. Total cholesterol, cholesterol ester, phospholipids, and triglycerides were increased in the plasma. Total cholesterol and cholesterol ester were also increased in the heart mitochondria and supernatant, and liver mitochondria, microsomes, and supernatant, but triglycerides and phospholipids in all cell fractions studied remained within the normal ranges. The respiratory control ratio, P/O ratio, and oxygen consumption of the cardiac and hepatic mitochondria were not affected by the increase in cholesterol content. Possible mechanisms responsible for the altered lipid composition in the cells of rabbits on a high-cholesterol diet, as well as that for the unchanged mitochondrial respiratory function are discussed.

Aspects of hepatic cholesterol metabolism in normal and dystrophic chicken embryos

1983 ◽  
Vol 61 (6) ◽  
pp. 378-386 ◽  
Author(s):  
D. E. Kuhn ◽  
D. M. Logan
Keyword(s):  
Total Cholesterol ◽  
Free Cholesterol ◽  
Cholesterol Metabolism ◽  
Cholesterol Content ◽  
Normal Embryo ◽  
Total Protein Content ◽  
Cholesterol Esters ◽  
Hepatic Cholesterol ◽  
Chicken Embryos ◽  
Dystrophic Chicken

Several aspects of hepatic cholesterol metabolism have been studied in normal and dystrophic chicken embryos (12 days in ovo). Dystrophic embryo livers weigh the same and have the same total protein content as their normal counterparts. However, the total cholesterol content of dystrophic embryo livers is significantly decreased compared with the content of normal embryo livers. This decrease in total cholesterol is due mainly to a large decrease in the amount of cholesterol esters, although the free cholesterol content is also decreased. The proportions of free cholesterol and cholesterol esters are 43 and 57%, respectively, in normal embryo livers, but this proportion is essentially reversed in dystrophic embryo livers (i.e., 56 and 44%). The decreased total cholesterol content of dystrophic embryo livers is not apparently due to a decrease in the rate of free cholesterol de novo biosynthesis or to a decrease in the rate of free cholesterol esterification. However, the decrease in cholesterol ester content may be due to an increase in the rate of its hydrolysis, as a result of increased levels of sterol ester hydrolase in dystrophic livers. These data are consistent with the proposal that membranes in dystrophic tissues are altered as a result of altered cholesterol metabolism and utilization.

Cholesterol in Serum and Lipoprotein Fractions

1956 ◽  
Vol 2 (3) ◽  
pp. 145-159 ◽  
Author(s):  
Joseph T Anderson ◽  
Ancel Keys
Keyword(s):  
Human Serum ◽  
Total Cholesterol ◽  
Filter Paper ◽  
Room Temperature ◽  
Paper Electrophoresis ◽  
Cholesterol Content ◽  
Intraindividual Variability ◽  
Detailed Data ◽  
The Stability ◽  
Source Of Error

Abstract 1. Methods are described for the separation, by paper electrophoresis and by cold ethanol, of α- and β-lipoproteins in 0.1 ml. of serum, with subsequent analysis of cholesterol in the separated portions. 2. It is shown that both methods of separation yield separated fractions containing substantially the same amounts of cholesterol. 3. Detailed data are given on the errors of measurement for total cholesterol and for cholesterol in the separated lipoprotein fractions. 4. Studies are reported on the stability of cholesterol in stored serum and on paper electrophoresis strips. It is shown that simple drying on filter paper causes no change in cholesterol content and yields a product that is stable for many weeks at ordinary room temperature. 5. The sources of variability in human serum cholesterol values are examined and it is shown that spontaneous intraindividual variability is a much greater source of error than the errors of measurement with these methods.

Total Cholesterol Content of Erythrocyte Membranes and Coronary Atherosclerosis: An Intravascular Ultrasound Pilot Study

Angiology ◽  
2009 ◽  
Vol 60 (6) ◽  
pp. 676-682 ◽  
Author(s):  
George D. Giannoglou ◽  
Konstantinos C. Koskinas ◽  
Dimitrios N. Tziakas ◽  
Antonios G. Ziakas ◽  
Antonios P. Antoniadis ◽  
...  
Keyword(s):  
Pilot Study ◽  
Total Cholesterol ◽  
Intravascular Ultrasound ◽  
Coronary Atherosclerosis ◽  
Cholesterol Content ◽  
Erythrocyte Membranes

Studies on the rapid cholesterol-depleting and steroidogenic actions of luteinizing hormone in the rat ovary: effects of aminoglutethimide phosphate

1970 ◽  
Vol 48 (8) ◽  
pp. 881-884 ◽  
Author(s):  
Harold R. Behrman ◽  
David T. Armstrong ◽  
Roy O. Greep
Keyword(s):  
Luteinizing Hormone ◽  
Cholesterol Ester ◽  
Free Cholesterol ◽  
Tissue Concentration ◽  
Control Level ◽  
Side Chain ◽  
Tissue Levels ◽  
Chain Cleavage ◽  
Luteal Tissue ◽  
Rat Ovary

Superovulated, immature rats were administered luteinizing hormone (LH) and/or aminoglutethimide phosphate (AGP), an inhibitor of cholesterol side-chain cleavage, to ascertain whether the steroidogenic action could be separated from the cholesterol-depleting action of LH. The injection of AGP [Formula: see text] and [Formula: see text] before sacrifice significantly reduced tissue levels of progesterone and 20α-dihydroprogesterone to 25–30% of normal, and increased tissue levels of free cholesterol in the absence and presence of LH given [Formula: see text] or 4 h before sacrifice. Cholesterol ester concentration was increased twofold after [Formula: see text] AGP, whereas LH injection [Formula: see text] after AGP and 4 h before sacrifice reduced the tissue concentration to the control level but not as low as that observed when only LH was given 4 h before sacrifice (50% of control level). The cholesterol ester depletion induced by LH, even though steroidogenesis was inhibited, indicates that these may represent separate events in the action of LH on luteal tissue.

scholarly journals Evaluation of the effect of a modified proprietary poultry feed on cholesterol excretion in laying birds

2019 ◽  
Vol 30 (2) ◽  
pp. 113-116
Author(s):  
Chris Oche Ikese ◽  
Simon Terver Ubwa ◽  
Sunday Ogakwu Adoga ◽  
Stephen Inegedu Audu ◽  
Michael Akor
Keyword(s):  
Total Cholesterol ◽  
Rice Bran ◽  
Colorimetric Method ◽  
Cholesterol Content ◽  
Poultry Feed ◽  
Control Group ◽  
Feeding Programs ◽  
Before And After ◽  
Cholesterol Excretion ◽  
The Mean

Abstract The effect of modified proprietary poultry feed on cholesterol excretion in laying birds was evaluated. The study sort to lower the cholesterol content of eggs at the point of their physiological formation via a modification of the proprietary feed fed to laying poultry birds. This was with a view to lower the net cholesterol content in their eggs, thereby lowering the cholesterol levels available in such eggs for intake upon consumption. A 20% rice bran modification of the proprietary feed was made. Animal subjects were divided into experimental and control groups and the mean total cholesterol in their faecal droppings where determined before and after varying their feeding programs. Whereas the experimental group was fed with the modified feed, the control group was maintained on the unmodified feed. Enzymatic colorimetric method was used for the determination of mean total cholesterol in the dried faecal droppings of each group of birds under investigation. The results showed that the mean total cholesterol excretion before and after the modified feed regimen were 5.97±0.16 mg/g and 9.99±0.47 mg/g respectively and were found to be significantly different when compared using a t-test at p > 0.05 and 49 degree of freedom The results also showed that the modified proprietary poultry feed increased total cholesterol excretion in the faecal droppings of laying birds fed with the modified feed by 67.3%, and this was found to be statistically significant at p > 0.05. Hence, a 20% rice-bran modification of the proprietary feed yields a modified feed with a proven potency in elevating total cholesterol excretion while producing no drastic deviation from the proximate composition of the unmodified proprietary feed and is thus likely to have no adverse effect on the productivity of laying birds.

scholarly journals Effect of mono and polyunsaturated fatty acids on muscle cholesterol level in fattening pigs

2005 ◽  
Vol 21 (1-2) ◽  
pp. 49-54 ◽  
Author(s):  
M. Pieszka ◽  
T. Barowicz ◽  
W. Migdalt ◽  
Branislav Zivkovic
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Total Cholesterol ◽  
Cholesterol Level ◽  
Linseed Oil ◽  
Cholesterol Content ◽  
Control Group ◽  
Longissimus Muscle ◽  
Fresh Tissue ◽  
Fattening Pigs

The effect of supplementing fattening pigs with oils differing in MUFA (monounusaturated fatty acids) and PUFA (polyunsaturated fatty acids) level addition for fatteners diets on the cholesterol content of m. longissimus was investigated. In a feeding trial with 48 crossbred pigs [? ($ White Polish Large x S Polish Landrace) x <$ Pietrain] randomly assigned to four treatments groups of 12 animals each (6 gilts and 6 barrows), dietary supplements of 3% maize, sunflower or linseed oil in the experimental groups and of 3% lard in the control group were applied. Total cholesterol in the longissimus muscle of linseed oil supplemented pigs was found to decrease significantly (PO.01) compared to the lard-fed pigs (60.75 vs. 71.37 mg/100 g of fresh tissue). Similar tendencies for reduced cholesterol level in longissimus muscle of sunflower and maize oil supplemented pigs were also noted. The production of pork with reduced total cholesterol seems to be important in terms of consumer health and culinary value.

THE INTERRELATION OF LIPIDS IN THE BLOOD PLASMA OF WHITE LEGHORN COCKERELS

1940 ◽  
Vol 18d (2) ◽  
pp. 49-52 ◽  
Author(s):  
Eldon M. Boyd ◽  
Eleanor L. Clarke
Keyword(s):  
Fatty Acids ◽  
Blood Plasma ◽  
Total Cholesterol ◽  
Total Lipid ◽  
Free Cholesterol ◽  
White Leghorn ◽  
Plasma Total Lipid ◽  
Total Fatty Acids

The blood plasma of 22 healthy, young, white leghorn cockerels was analysed by oxidative micromethods and found to contain the following mean lipid values, in milligrams per 100 ml. of plasma: total lipid 520, neutral fat 225, total fatty acids 361, total cholesterol 100, ester cholesterol 66, free cholesterol 34, phospholipid 155. In general, the amounts of the various lipids were proportionately related to the total lipid.

Lipid changes in the perfusates of isolated rabbit hearts

1960 ◽  
Vol 198 (5) ◽  
pp. 1115-1118 ◽  
Author(s):  
Harvey I. Miller ◽  
Paul S. Roheim ◽  
John J. Spitzer
Keyword(s):  
Fatty Acids ◽  
Oleic Acid ◽  
Total Cholesterol ◽  
Free Cholesterol ◽  
Perfusion Medium ◽  
Esterified Fatty Acids ◽  
Before And After ◽  
Lipid Components

Rabbit hearts were perfused with different media using the Langendorff technique. Concentrations of the different lipid components were determined before and after perfusion. In experiments using hypercholesterolemic serums to perfuse normal hearts the concentrations of unesterified fatty acids (UFA) in the perfusates were above control levels, and esterified fatty acids were decreased. Total cholesterol did not change, free cholesterol increased, thus esterified cholesterol decreased. In about 50% of the experiments in which oleic acid-loaded albumin was used to perfuse normal hearts, an uptake of UFA occurred. Triglycerides were apparently not removed by rabbit hearts even when hypertriglycerolemic serum was the perfusion medium.

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