Lipid composition of the subcellular elements, and mitochondrial respiration of the heart and liver in hypercholesterolemic rabbits

1968 ◽  
Vol 46 (6) ◽  
pp. 823-828 ◽  
Author(s):  
C. C. Huang ◽  
K. Kako
Keyword(s):  
Total Cholesterol ◽  
Lipid Composition ◽  
Cholesterol Ester ◽  
Respiratory Control ◽  
Cholesterol Content ◽  
Liver Mitochondria ◽  
High Cholesterol Diet ◽  
Normal Ranges ◽  
Cell Fractions ◽  
Altered Lipid

Rabbits were fed a diet containing 3% cholesterol for 2–6 months. Total cholesterol, cholesterol ester, phospholipids, and triglycerides were increased in the plasma. Total cholesterol and cholesterol ester were also increased in the heart mitochondria and supernatant, and liver mitochondria, microsomes, and supernatant, but triglycerides and phospholipids in all cell fractions studied remained within the normal ranges. The respiratory control ratio, P/O ratio, and oxygen consumption of the cardiac and hepatic mitochondria were not affected by the increase in cholesterol content. Possible mechanisms responsible for the altered lipid composition in the cells of rabbits on a high-cholesterol diet, as well as that for the unchanged mitochondrial respiratory function are discussed.

Inhibition of Platelet Aggregation by Ethanol in Vitro Shows Specificity for Aggregating Agent Used and Is Influenced by Platelet Lipid Composition

1982 ◽  
Vol 48 (01) ◽  
pp. 049-053 ◽  
Author(s):  
C G Fenn ◽  
J M Littleton
Keyword(s):  
Platelet Aggregation ◽  
Lipid Composition ◽  
Saturated Fatty Acids ◽  
Calcium Ionophore ◽  
Cholesterol Content ◽  
Membrane Lipid ◽  
Calcium Ionophore A23187 ◽  
Ionophore A23187 ◽  
Increased Sensitivity

SummaryEthanol at physiologically tolerable concentrations inhibited platelet aggregation in vitro in a relatively specific way, which may be influenced by platelet membrane lipid composition. Aggregation to collagen, calcium ionophore A23187 and thrombin (low doses) were often markedly inhibited by ethanol, adrenaline and ADP responses were little affected, and aggregation to exogenous arachidonic acid was actually potentiated by ethanol. Aggregation to collagen, thrombin and A23187 was inhibited more by ethanol in platelets enriched with saturated fatty acids than in those enriched with unsaturated fats. Platelets enriched with cholesterol showed increased sensitivity to ADP, arachidonate and adrenaline but this increase in cholesterol content did not appear to influence the inhibition by ethanol of platelet responses. The results suggest that ethanol may inhibit aggregation by an effect on membrane fluidity and/or calcium mobilization resulting in decreased activity of a membrane-bound phospholipase.

Cholesterol in Serum and Lipoprotein Fractions

1956 ◽  
Vol 2 (3) ◽  
pp. 145-159 ◽  
Author(s):  
Joseph T Anderson ◽  
Ancel Keys
Keyword(s):  
Human Serum ◽  
Total Cholesterol ◽  
Filter Paper ◽  
Room Temperature ◽  
Paper Electrophoresis ◽  
Cholesterol Content ◽  
Intraindividual Variability ◽  
Detailed Data ◽  
The Stability ◽  
Source Of Error

Abstract 1. Methods are described for the separation, by paper electrophoresis and by cold ethanol, of α- and β-lipoproteins in 0.1 ml. of serum, with subsequent analysis of cholesterol in the separated portions. 2. It is shown that both methods of separation yield separated fractions containing substantially the same amounts of cholesterol. 3. Detailed data are given on the errors of measurement for total cholesterol and for cholesterol in the separated lipoprotein fractions. 4. Studies are reported on the stability of cholesterol in stored serum and on paper electrophoresis strips. It is shown that simple drying on filter paper causes no change in cholesterol content and yields a product that is stable for many weeks at ordinary room temperature. 5. The sources of variability in human serum cholesterol values are examined and it is shown that spontaneous intraindividual variability is a much greater source of error than the errors of measurement with these methods.

Total Cholesterol Content of Erythrocyte Membranes and Coronary Atherosclerosis: An Intravascular Ultrasound Pilot Study

Angiology ◽  
2009 ◽  
Vol 60 (6) ◽  
pp. 676-682 ◽  
Author(s):  
George D. Giannoglou ◽  
Konstantinos C. Koskinas ◽  
Dimitrios N. Tziakas ◽  
Antonios G. Ziakas ◽  
Antonios P. Antoniadis ◽  
...  
Keyword(s):  
Pilot Study ◽  
Total Cholesterol ◽  
Intravascular Ultrasound ◽  
Coronary Atherosclerosis ◽  
Cholesterol Content ◽  
Erythrocyte Membranes

Abstract 277: Microrna-33 Promotes Cardiac Fibrosis by Maintaining Cellular Lipid Homeostasis

2016 ◽  
Vol 119 (suppl_1) ◽  
Author(s):  
Masataka Nishiga ◽  
Takahiro Horie ◽  
Yasuhide Kuwabara ◽  
Osamu Baba ◽  
Tetsushi Nakao ◽  
...  
Keyword(s):  
Cardiac Fibrosis ◽  
Cardiac Fibroblasts ◽  
Pressure Overload ◽  
Cholesterol Content ◽  
Atp Binding Cassette Transporter ◽  
Primary Fibroblasts ◽  
Proliferation In Vitro ◽  
Altered Lipid

Background: A highly conserved microRNA, miR-33 is considered as a potential therapeutic target for atherosclerosis, because recent reports, including ours, indicated miR-33 has atherogenic effects by reducing HDL-C. However, the functions of miR-33 in heart failure remain to be elucidated. Methods and results: To clarify the functions of miR-33 involved in cardiac hypertrophy and fibrosis in vivo, we investigated the responses to pressure overload by transverse aortic constriction (TAC) in miR-33 deficient (KO) mice. When subjected to TAC, miR-33 expression level was significantly up-regulated in wild-type (WT) left ventricles, whereas miR-33 KO hearts displayed no less hypertrophic responses than WT hearts. However, interestingly, histological and gene expression analyses showed ameliorated cardiac fibrosis in miR-33 KO hearts compared to WT hearts. Furthermore, we generated cardiac fibroblast specific miR-33 deficient mice, which also showed ameliorated cardiac fibrosis when they were subjected to TAC. We also found that cardiac fibroblasts were mainly responsible for miR-33 expression in the heart, because its expression was about 4-folds higher in isolated primary cardiac fibroblasts than cardiomyocytes. Deficiency of miR-33 impaired cell proliferation in primary fibroblasts, which was considered due to altered lipid raft cholesterol content by up-regulated ATP-binding cassette transporter A1/G1. Conclusion: Deficiency of miR-33 impaired fibroblast proliferation in vitro, and ameliorated cardiac fibrosis induced by pressure overload in vivo.

Dietary Taurine Decreases Hepatic Secretion of Cholesterol Ester in Rats Fed a High-Cholesterol Diet

Pharmacology ◽  
2000 ◽  
Vol 60 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Kyosuke Yamamoto ◽  
Ayako Yoshitama ◽  
Masanobu Sakono ◽  
Tetsuo Nasu ◽  
Shigeru Murakami ◽  
...  
Keyword(s):  
Cholesterol Ester ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol

scholarly journals Some Evidences on Effect of Intake Aguamiel (Agave sap)

2016 ◽  
Vol 5 (1) ◽  
pp. 49
Author(s):  
A. Carrillo-López ◽  
H. Silos-Espino ◽  
S. Flores-Benitez ◽  
E. A. Espinoza-Sánchez ◽  
J. R. Ornelas-Tavares ◽  
...  
Keyword(s):  
Blood Cell ◽  
Total Cholesterol ◽  
Cell Count ◽  
Adult Males ◽  
Blood Cell Count ◽  
Folk Belief ◽  
Young Males ◽  
Cholesterol Levels ◽  
Normal Ranges ◽  
Related Proteins

Honeywater or aguamiel (Agave sap) has been consumed by Mexican population since pre-columbian times. Although, it has been claimed by folk belief that aguamiel possesses some medicinal properties, scientific studies on its effect on human health have not been well documented. The behavior of blood components in nine volunteers (two young males, three adult females and four adult males) after aguamiel consumption (250 mL every three days during a period of 35 days) was analyzed. It was found that, serum red blood cell count, serum white blood cell count, platelet count, minerals (Zn, Mg and Fe) and iron-related proteins (ferritin and transferrin) levels were not negatively affected because of all of these blood indicators ranged within normal reference values. However, this study showed that aguamiel presented a specific functional effect since hypercholesterolemic adult males showed normal levels of serum total cholesterol after aguamiel consumption, whereas total cholesterol levels were kept in normal ranges after aguamiel consumption for normocholesterolemic subjects. Furthermore, aguamiel consumption did not cause hyperglycemia in any of the tested groups.

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