scholarly journals Distinction of Gram-positive and -negative bacteria using a colorimetric microbial viability assay based on the reduction of water-soluble tetrazolium salts with a selection medium

2011 ◽  
Vol 57 (6) ◽  
pp. 331-339 ◽  
Author(s):  
Tadayuki Tsukatani ◽  
Hikaru Suenaga ◽  
Tomoko Higuchi ◽  
Masanobu Shiga ◽  
Katsuya Noguchi ◽  
...  
Keyword(s):  
Water Soluble ◽  
Gram Positive ◽  
Tetrazolium Salts ◽  
Viability Assay ◽  
Microbial Viability ◽  
Selection Medium

Determination of water-soluble vitamins using a colorimetric microbial viability assay based on the reduction of water-soluble tetrazolium salts

2011 ◽  
Vol 127 (2) ◽  
pp. 711-715 ◽  
Author(s):  
Tadayuki Tsukatani ◽  
Hikaru Suenaga ◽  
Munetaka Ishiyama ◽  
Takatoshi Ezoe ◽  
Kiyoshi Matsumoto
Keyword(s):  
Water Soluble ◽  
Tetrazolium Salts ◽  
Viability Assay ◽  
Microbial Viability

scholarly journals Novel Type of Water-Soluble Photosensitizer from Trichoderma reesei for Photodynamic Inactivation of Gram-Positive Bacteria

Langmuir ◽  
2020 ◽  
Vol 36 (44) ◽  
pp. 13227-13235
Author(s):  
Zihuayuan Yang ◽  
Ying Qiao ◽  
Junying Li ◽  
Fu-Gen Wu ◽  
Fengming Lin
Keyword(s):  
Trichoderma Reesei ◽  
Water Soluble ◽  
Photodynamic Inactivation ◽  
Gram Positive ◽  
Gram Positive Bacteria

Colorimetric cell proliferation assay for microorganisms in microtiter plate using water-soluble tetrazolium salts

2008 ◽  
Vol 75 (1) ◽  
pp. 109-116 ◽  
Author(s):  
Tadayuki Tsukatani ◽  
Hikaru Suenaga ◽  
Tomoko Higuchi ◽  
Tetsuyuki Akao ◽  
Munetaka Ishiyama ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Microtiter Plate ◽  
Water Soluble ◽  
Proliferation Assay ◽  
Cell Proliferation Assay ◽  
Tetrazolium Salts

scholarly journals Benzothiazole-Containing Tetrazolium Salts That Produce Water-Soluble Formazan Dyes Absorbing at a Long Wavelength upon NADH Reduction.

1996 ◽  
Vol 12 (3) ◽  
pp. 515-519 ◽  
Author(s):  
Munetaka ISHIYAMA ◽  
Yoko MIYAZONO ◽  
Masanobu SHIGA ◽  
Kazumi SASAMOTO ◽  
Yosuke OHKURA ◽  
...  
Keyword(s):  
Water Soluble ◽  
Produce Water ◽  
Long Wavelength ◽  
Tetrazolium Salts

scholarly journals A Novel Water-Soluble Photosensitizer for Photodynamic Inactivation of Gram-Positive Bacteria

2020 ◽  
Author(s):  
Zihuayuan Yang ◽  
Ying Qiao ◽  
Junying Li ◽  
Fu-Gen Wu ◽  
Fengming Lin
Keyword(s):  
Photodynamic Therapy ◽  
Water Solubility ◽  
Uv Light ◽  
Water Soluble ◽  
Light Irradiation ◽  
Microbial Fermentation ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Uv Light Irradiation

AbstractAntimicrobial photodynamic therapy (APDT) is a promising alternative to traditional antibiotics for bacterial infections, which inactivates a broad spectrum of bacteria. However, it has some disadvantages including poor water solubility and easy aggregation of hydrophobic photosensitizers (PS), and poor tissue penetration and cytotoxicity when using UV as the light source, leading to undesired photodynamic therapy efficacy. Herein, we develop a novel water-soluble natural PS (sorbicillinoids) obtained by microbial fermentation using recombinant filamentous fungus Trichoderma reesei (T. reesei). Sorbicillinoids could effectively generate singlet oxygen (1O2) under ultraviolet (UV) light irradiation, and ultimately display photoinactivation activity on Gram-positive bacteria, but not Gram-negative ones. Staphylococcus aureus (S. aureus) treated with sorbicillinoids and UV light displayed high levels of intracellular reactive oxygen species (ROS), notable DNA photocleavage, and compromised cell semi-permeability without overt cell membrane disruption. Moreover, the dark toxicity, phototoxicity or hemolysis activity of sorbicillinoids is negligible, showing its excellent biocompatibility. Therefore, sorbicillinoids, a type of secondary metabolite from fungus, has a promising future as a new PS for APDT using nontoxic dose of UV light irradiation.ImportanceIt is of great value to develop novel PSs for APDT to enhance its efficacy for the reason that many traditional PSs have disadvantages like low water solubility and poor biocompatibility. In this study, we develop a novel water-soluble natural PS - sorbicillinoids obtained by microbial fermentation using T. reesei. Sorbicillinoids could effectively generate singlet oxygen under UV light irradiation, and ultimately display photoinactivation activity on Gram-positive bacteria, but not Gram-negative ones. More importantly, UV light can generally only be used to inactivate bacteria on the surface due to its weak penetration. However, it can penetrate deep into the solution and inactivate bacteria in the presence of sorbicillinoids. Therefore, sorbicillinoids, a type of secondary metabolite from fungus, has a promising future as a new PS for APDT using nontoxic dose of UV light irradiation.

Sign in / Sign up

Export Citation Format

Share Document